CSGLCAT (CHPF2) (NM_019015) Human Tagged ORF Clone

CAT#: RC214117

CHPF2 (Myc-DDK-tagged)-Human chondroitin polymerizing factor 2 (CHPF2)

Product Images

Circular map for RC214117

• 

  Western blot validation of overexpression lysate (Cat# LY412809) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214117 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CSGLCAT
• Synonyms: chPF-2; ChSy-3; CSGLCA-T; CSGlcAT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214117 representing NM_019015
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGACTGAGCTCCCTGTTGGCTCTGCTGCGGCCAGCGCTTCCCCTCATCTTAGGGCTGTCTCTGGGGT
  GCAGCCTGAGCCTCCTGCGGGTTTCCTGGATCCAGGGGGAGGGAGAAGATCCCTGTGTCGAGGCTGTAGG
  GGAGCGAGGAGGGCCACAGAATCCAGATTCCAGAGCTCGGCTAGACCAAAGTGATGAAGACTTCAAACCC
  CGGATTGTCCCCTACTACAGGGACCCCAACAAGCCCTACAAGAAGGTGCTCAGGACTCGGTACATCCAGA
  CAGAGCTGGGCTCCCGTGAGCGGTTGCTGGTGGCTGTCCTGACCTCCCGAGCTACACTGTCCACTTTGGC
  CGTGGCTGTGAACCGTACGGTGGCCCATCACTTCCCTCGGTTACTCTACTTCACTGGGCAGCGGGGGGCC
  CGGGCTCCAGCAGGGATGCAGGTGGTGTCTCATGGGGATGAGCGGCCCGCCTGGCTCATGTCAGAGACCC
  TGCGCCACCTTCACACACACTTTGGGGCCGACTACGACTGGTTCTTCATCATGCAGGATGACACATATGT
  GCAGGCCCCCCGCCTGGCAGCCCTTGCTGGCCACCTCAGCATCAACCAAGACCTGTACTTAGGCCGGGCA
  GAGGAGTTCATTGGCGCAGGCGAGCAGGCCCGGTACTGTCATGGGGGCTTTGGCTACCTGTTGTCACGGA
  GTCTCCTGCTTCGTCTGCGGCCACATCTGGATGGCTGCCGAGGAGACATTCTCAGTGCCCGTCCTGACGA
  GTGGCTTGGACGCTGCCTCATTGACTCTCTGGGCGTCGGCTGTGTCTCACAGCACCAGGGGCAGCAGTAT
  CGCTCATTTGAACTGGCCAAAAATAGGGACCCTGAGAAGGAAGGGAGCTCGGCTTTCCTGAGTGCCTTCG
  CCGTGCACCCTGTCTCCGAAGGTACCCTCATGTACCGGCTCCACAAACGCTTCAGCGCTCTGGAGTTGGA
  GCGGGCTTACAGTGAAATAGAACAACTGCAGGCTCAGATCCGGAACCTGACCGTGCTGACCCCCGAAGGG
  GAGGCAGGGCTGAGCTGGCCCGTTGGGCTCCCTGCTCCTTTCACACCACACTCTCGCTTTGAGGTGCTGG
  GCTGGGACTACTTCACAGAGCAGCACACCTTCTCCTGTGCAGATGGGGCTCCCAAGTGCCCACTACAGGG
  GGCTAGCAGGGCGGACGTGGGTGATGCGTTGGAGACTGCCCTGGAGCAGCTCAATCGGCGCTATCAGCCC
  CGCCTGCGCTTCCAGAAGCAGCGACTGCTCAACGGCTATCGGCGCTTCGACCCAGCACGGGGCATGGAGT
  ACACCCTGGACCTGCTGTTGGAATGTGTGACACAGCGTGGGCACCGGCGGGCCCTGGCTCGCAGGGTCAG
  CCTGCTGCGGCCACTGAGCCGGGTGGAAATCCTACCTATGCCCTATGTCACTGAGGCCACCCGAGTGCAG
  CTGGTGCTGCCACTCCTGGTGGCTGAAGCTGCTGCAGCCCCGGCTTTCCTCGAGGCCTTTGCAGCCAATG
  TCCTGGAGCCACGAGAACATGCATTGCTCACCCTGTTGCTGGTCTACGGGCCACGAGAAGGTGGCCGTGG
  AGCTCCAGACCCATTTCTTGGGGTGAAGGCTGCAGCAGCGGAGTTAGAGCGACGGTACCCTGGGACGAGG
  CTGGCCTGGCTCGCTGTGCGAGCAGAGGCCCCTTCCCAGGTGCGACTCATGGACGTGGTCTCGAAGAAGC
  ACCCTGTGGACACTCTCTTCTTCCTTACCACCGTGTGGACAAGGCCTGGGCCCGAAGTCCTCAACCGCTG
  TCGCATGAATGCCATCTCTGGCTGGCAGGCCTTCTTTCCAGTCCATTTCCAGGAGTTCAATCCTGCCCTG
  TCACCACAGAGATCACCCCCAGGGCCCCCGGGGGCTGGCCCTGACCCCCCCTCCCCTCCTGGTGCTGACC
  CCTCCCGGGGGGCTCCTATAGGGGGGAGATTTGACCGGCAGGCTTCTGCGGAGGGCTGCTTCTACAACGC
  TGACTACCTGGCGGCCCGAGCCCGGCTGGCAGGTGAACTGGCAGGCCAGGAAGAGGAGGAAGCCCTGGAG
  GGGCTGGAGGTGATGGATGTTTTCCTCCGGTTCTCAGGGCTCCACCTCTTTCGGGCCGTAGAGCCAGGGC
  TGGTGCAGAAGTTCTCCCTGCGAGACTGCAGCCCACGGCTCAGTGAAGAACTCTACCACCGCTGCCGCCT
  CAGCAACCTGGAGGGGCTAGGGGGCCGTGCCCAGCTGGCTATGGCTCTCTTTGAGCAGGAGCAGGCCAAT
  AGCACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214117 representing NM_019015
  Red=Cloning site Green=Tags(s)
  
  MRLSSLLALLRPALPLILGLSLGCSLSLLRVSWIQGEGEDPCVEAVGERGGPQNPDSRARLDQSDEDFKP
  RIVPYYRDPNKPYKKVLRTRYIQTELGSRERLLVAVLTSRATLSTLAVAVNRTVAHHFPRLLYFTGQRGA
  RAPAGMQVVSHGDERPAWLMSETLRHLHTHFGADYDWFFIMQDDTYVQAPRLAALAGHLSINQDLYLGRA
  EEFIGAGEQARYCHGGFGYLLSRSLLLRLRPHLDGCRGDILSARPDEWLGRCLIDSLGVGCVSQHQGQQY
  RSFELAKNRDPEKEGSSAFLSAFAVHPVSEGTLMYRLHKRFSALELERAYSEIEQLQAQIRNLTVLTPEG
  EAGLSWPVGLPAPFTPHSRFEVLGWDYFTEQHTFSCADGAPKCPLQGASRADVGDALETALEQLNRRYQP
  RLRFQKQRLLNGYRRFDPARGMEYTLDLLLECVTQRGHRRALARRVSLLRPLSRVEILPMPYVTEATRVQ
  LVLPLLVAEAAAAPAFLEAFAANVLEPREHALLTLLLVYGPREGGRGAPDPFLGVKAAAAELERRYPGTR
  LAWLAVRAEAPSQVRLMDVVSKKHPVDTLFFLTTVWTRPGPEVLNRCRMNAISGWQAFFPVHFQEFNPAL
  SPQRSPPGPPGAGPDPPSPPGADPSRGAPIGGRFDRQASAEGCFYNADYLAARARLAGELAGQEEEEALE
  GLEVMDVFLRFSGLHLFRAVEPGLVQKFSLRDCSPRLSEELYHRCRLSNLEGLGGRAQLAMALFEQEQAN
  ST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019015
• ORF Size: 2316 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019015.2, NP_061888.1
• RefSeq Size: 3970 bp
• RefSeq ORF: 2319 bp
• Locus ID: 54480
• UniProt ID: Q9P2E5
• Cytogenetics: 7q36.1
• Protein Pathways: Chondroitin sulfate biosynthesis, Metabolic pathways
• MW: 85.8 kDa
• Gene Summary: Transfers glucuronic acid (GlcUA) from UDP-GlcUA to N-acetylgalactosamine residues on the non-reducing end of the elongating chondroitin polymer. Has no N-acetylgalactosaminyltransferase activity.[UniProtKB/Swiss-Prot Function]