HCN2 (NM_001194) Human Tagged ORF Clone

CAT#: RC214036

HCN2 (Myc-DDK-tagged)-Human hyperpolarization activated cyclic nucleotide-gated potassium channel 2 (HCN2)

Product Images

Circular map for RC214036

• 

  Western blot validation of overexpression lysate (Cat# LY420072) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214036 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HCN2
• Synonyms: BCNG-2; BCNG2; HAC-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214036 representing NM_001194
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCGCGCGGGGGCGGCGGGCGGCCCGGGGAGAGCCCGGGCGCGACCCCCGCGCCGGGGCCGCCGC
  CGCCGCCGCCGCCCGCGCCCCCCCAACAGCAGCCGCCGCCGCCGCCGCCGCCCGCGCCCCCCCCGGGCCC
  CGGGCCCGCGCCCCCCCAGCACCCGCCCCGGGCCGAGGCGTTGCCCCCGGAGGCGGCGGATGAGGGCGGC
  CCGCGGGGCCGGCTCCGCAGCCGCGACAGCTCGTGCGGCCGCCCCGGCACCCCGGGCGCGGCGAGCACGG
  CCAAGGGCAGCCCGAACGGCGAGTGCGGGCGCGGCGAGCCGCAGTGCAGCCCCGCGGGGCCCGAGGGCCC
  GGCGCGGGGGCCCAAGGTGTCGTTCTCGTGCCGCGGGGCGGCCTCGGGGCCCGCGCCGGGGCCGGGGCCG
  GCGGAGGAGGCGGGCAGCGAGGAGGCGGGCCCGGCGGGGGAGCCGCGCGGCAGCCAGGCCAGCTTCATGC
  AGCGCCAGTTCGGCGCGCTCCTGCAGCCGGGCGTCAACAAGTTCTCGCTGCGGATGTTCGGCAGCCAGAA
  GGCCGTGGAGCGCGAGCAGGAGCGCGTCAAGTCGGCGGGGGCCTGGATCATCCACCCGTACAGCGACTTC
  AGGTTCTACTGGGACTTCACCATGCTGCTGTTCATGGTGGGAAACCTCATCATCATCCCAGTGGGCATCA
  CCTTCTTCAAGGATGAGACCACTGCCCCGTGGATCGTGTTCAACGTGGTCTCGGACACCTTCTTCCTCAT
  GGACCTGGTGTTGAACTTCCGCACCGGCATTGTGATCGAGGACAACACGGAGATCATCCTGGACCCCGAG
  AAGATCAAGAAGAAGTATCTGCGCACGTGGTTCGTGGTGGACTTCGTGTCCTCCATCCCCGTGGACTACA
  TCTTCCTTATTGTGGAGAAGGGCATTGACTCCGAGGTCTACAAGACGGCACGTGCCCTGCGCATCGTGCG
  CTTCACCAAGATCCTCAGCCTCCTGCGGCTGCTGCGCCTCTCACGCCTGATCCGCTACATCCATCAGTGG
  GAGGAGATCTTCCACATGACCTATGACCTGGCCAGCGCGGTGATGAGGATCTGCAATCTCATCAGCATGA
  TGCTGCTGCTCTGCCACTGGGACGGCTGCCTGCAGTTCCTGGTGCCTATGCTGCAGGACTTCCCGCGCAA
  CTGCTGGGTGTCCATCAATGGCATGGTGAACCACTCGTGGAGTGAACTGTACTCCTTCGCACTCTTCAAG
  GCCATGAGCCACATGCTGTGCATCGGGTACGGCCGGCAGGCGCCCGAGAGCATGACGGACATCTGGCTGA
  CCATGCTCAGCATGATTGTGGGTGCCACCTGCTACGCCATGTTCATCGGCCACGCCACTGCCCTCATCCA
  GTCGCTGGACTCCTCGCGGCGCCAGTACCAGGAGAAGTACAAGCAGGTGGAGCAGTACATGTCCTTCCAC
  AAGCTGCCAGCTGACTTCCGCCAGAAGATCCACGACTACTATGAGCACCGTTACCAGGGCAAGATGTTTG
  ACGAGGACAGCATCCTGGGCGAGCTCAACGGGCCCCTGCGGGAGGAGATCGTCAACTTCAACTGCCGGAA
  GCTGGTGGCCTCCATGCCGCTGTTCGCCAACGCCGACCCCAACTTCGTCACGGCCATGCTGACCAAGCTC
  AAGTTCGAGGTCTTCCAGCCGGGTGACTACATCATCCGCGAAGGCACCATCGGGAAGAAGATGTACTTCA
  TCCAGCACGGCGTGGTCAGCGTGCTCACTAAGGGCAACAAGGAGATGAAGCTGTCCGATGGCTCCTACTT
  CGGGGAGATCTGCCTGCTCACCCGGGGCCGCCGCACGGCGAGCGTGCGGGCCGACACCTACTGCCGCCTC
  TATTCGCTGAGCGTGGACAACTTCAACGAGGTGCTGGAGGAGTACCCCATGATGCGGCGCGCCTTCGAGA
  CGGTGGCCATCGACCGCCTGGACCGCATCGGCAAGAAGAATTCCATCCTCCTGCACAAGGTGCAGCATGA
  CCTCAACTCGGGCGTATTCAACAACCAGGAGAACGCCATCATCCAGGAGATCGTCAAGTACGACCGCGAG
  ATGGTGCAGCAGGCCGAGCTGGGTCAGCGCGTGGGCCTCTTCCCGCCGCCGCCGCCGCCGCCGCAGGTCA
  CCTCGGCCATCGCCACGCTGCAGCAGGCGGCGGCCATGAGCTTCTGCCCGCAGGTGGCGCGGCCGCTCGT
  GGGGCCGCTGGCGCTCGGCTCGCCGCGCCTCGTGCGCCGCCCGCCCCCGGGGCCCGCACCTGCCGCCGCC
  TCACCCGGGCCCCCGCCCCCCGCCAGCCCCCCGGGCGCGCCCGCCAGCCCCCGGGCACCGCGGACCTCGC
  CCTACGGCGGCCTGCCCGCCGCCCCCCTTGCTGGGCCCGCCCTGCCCGCGCGCCGCCTGAGCCGCGCGTC
  GCGCCCACTGTCCGCCTCGCAGCCCTCGCTGCCTCACGGCGCCCCCGGCCCCGCGGCCTCCACACGCCCG
  GCCAGCAGCTCCACACCGCGCTTGGGGCCCACGCCCGCTGCCCGGGCCGCCGCGCCCAGCCCGGACCGCA
  GGGACTCGGCCTCACCCGGCGCCGCCGGCGGCCTGGACCCCCAGGACTCCGCGCGCTCGCGCCTCTCGTC
  CAACTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214036 representing NM_001194
  Red=Cloning site Green=Tags(s)
  
  MDARGGGGRPGESPGATPAPGPPPPPPPAPPQQQPPPPPPPAPPPGPGPAPPQHPPRAEALPPEAADEGG
  PRGRLRSRDSSCGRPGTPGAASTAKGSPNGECGRGEPQCSPAGPEGPARGPKVSFSCRGAASGPAPGPGP
  AEEAGSEEAGPAGEPRGSQASFMQRQFGALLQPGVNKFSLRMFGSQKAVEREQERVKSAGAWIIHPYSDF
  RFYWDFTMLLFMVGNLIIIPVGITFFKDETTAPWIVFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPE
  KIKKKYLRTWFVVDFVSSIPVDYIFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQW
  EEIFHMTYDLASAVMRICNLISMMLLLCHWDGCLQFLVPMLQDFPRNCWVSINGMVNHSWSELYSFALFK
  AMSHMLCIGYGRQAPESMTDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFH
  KLPADFRQKIHDYYEHRYQGKMFDEDSILGELNGPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKL
  KFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRL
  YSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLHKVQHDLNSGVFNNQENAIIQEIVKYDRE
  MVQQAELGQRVGLFPPPPPPPQVTSAIATLQQAAAMSFCPQVARPLVGPLALGSPRLVRRPPPGPAPAAA
  SPGPPPPASPPGAPASPRAPRTSPYGGLPAAPLAGPALPARRLSRASRPLSASQPSLPHGAPGPAASTRP
  ASSSTPRLGPTPAARAAAPSPDRRDSASPGAAGGLDPQDSARSRLSSNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001194
• ORF Size: 2667 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001194.4
• RefSeq Size: 3459 bp
• RefSeq ORF: 2670 bp
• Locus ID: 610
• UniProt ID: Q9UL51
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, Ion Channels: Cyclic nucleotide gated, Transmembrane
• MW: 96.8 kDa
• Gene Summary: The protein encoded by this gene is a hyperpolarization-activated cation channel involved in the generation of native pacemaker activity in the heart and in the brain. The encoded protein is activated by cAMP and can produce a fast, large current. Defects in this gene were noted as a possible cause of some forms of epilepsy. [provided by RefSeq, Jan 2017]