TRIM17 (NM_016102) Human Tagged ORF Clone

CAT#: RC213994

TRIM17 (Myc-DDK-tagged)-Human tripartite motif containing 17 (TRIM17), transcript variant 1

Product Images

Circular map for RC213994

• 

  Western blot validation of overexpression lysate (Cat# LY422560) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216352 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIM17
• Synonyms: RBCC; RNF16; terf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213994 representing NM_016102
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCTGTGGAACTCGCCAGAAAACTGCAGGAGGAAGCTACGTGCTCCATCTGTCTGGATTACTTCA
  CAGACCCTGTGATGACCACCTGTGGCCACAACTTCTGCCGAGCCTGCATCCAGCTGAGCTGGGAAAAGGC
  GAGGGGCAAGAAGGGGAGGCGGAAGCGGAAGGGCTCCTTCCCCTGCCCCGAGTGCAGAGAGATGTCCCCG
  CAGAGGAACCTGCTGCCCAACCGGCTGCTGACCAAGGTGGCCGAGATGGCGCAGCAGCATCCTGGTCTGC
  AGAAGCAAGACCTGTGCCAGGAGCACCACGAGCCCCTCAAGCTTTTCTGCCAGAAGGACCAGAGCCCCAT
  CTGTGTGGTGTGCAGGGAGTCCCGGGAGCACCGGCTGCACAGGGTGCTGCCCGCCGAGGAGGCAGTGCAG
  GGGTACAAGTTGAAGCTGGAGGAGGACATGGAGTACCTTCGGGAGCAGATCACCAGGACAGGGAATCTGC
  AGGCCAGGGAGGAGCAGAGCTTAGCCGAGTGGCAGGGCAAGGTGAAGGAGCGGAGAGAACGCATTGTGCT
  GGAGTTTGAGAAGATGAACCTCTACCTGGTGGAAGAAGAGCAGAGGCTCCTCCAGGCTCTGGAGACGGAA
  GAAGAGGAGACTGCCAGCAGGCTCCGGGAGAGCGTGGCCTGCCTGGACCGGCAGGGTCACTCTCTGGAGC
  TGCTGCTGCTGCAGCTGGAGGAGCGGAGCACACAGGGGCCCCTCCAGATGCTGCAGGACATGAAGGAACC
  CCTGAGCAGGAAGAACAACGTGAGTGTGCAGTGCCCAGAGGTTGCCCCCCCAACCAGACCCAGGACTGTG
  TGCAGAGTTCCCGGACAGATTGAAGTGCTAAGAGGCTTTCTAGAGGATGTGGTGCCTGATGCCACCTCCG
  CGTACCCCTACCTCCTCCTGTATGAGAGCCGCCAGAGGCGCTACCTCGGCTCTTCGCCGGAGGGCAGTGG
  GTTCTGCAGCAAGGACCGATTTGTGGCTTACCCCTGTGCTGTGGGCCAGACGGCCTTCTCCTCTGGGAGG
  CACTACTGGGAGGTGGGCATGAACATCACCGGGGACGCGTTGTGGGCCCTGGGTGTGTGCAGGGACAACG
  TGAGCCGGAAAGACAGGGTCCCCAAGTGCCCCGAAAACGGCTTCTGGGTGGTGCAGCTGTCCAAGGGGAC
  CAAGTACTTATCCACCTTCTCTGCCCTAACCCCGGTCATGCTGATGGAGCCTCCCAGCCACATGGGCATC
  TTCCTGGACTTCGAAGCCGGGGAAGTGTCCTTCTACAGTGTAAGCGATGGGTCCCACCTGCACACCTACT
  CCCAGGCCACCTTCCCAGGCCCCCTGCAGCCTTTCTTCTGCCTGGGGGCTCCGAAGTCTGGTCAGATGGT
  CATCTCCACAGTGACCATGTGGGTGAAAGGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213994 representing NM_016102
  Red=Cloning site Green=Tags(s)
  
  MEAVELARKLQEEATCSICLDYFTDPVMTTCGHNFCRACIQLSWEKARGKKGRRKRKGSFPCPECREMSP
  QRNLLPNRLLTKVAEMAQQHPGLQKQDLCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLPAEEAVQ
  GYKLKLEEDMEYLREQITRTGNLQAREEQSLAEWQGKVKERRERIVLEFEKMNLYLVEEEQRLLQALETE
  EEETASRLRESVACLDRQGHSLELLLLQLEERSTQGPLQMLQDMKEPLSRKNNVSVQCPEVAPPTRPRTV
  CRVPGQIEVLRGFLEDVVPDATSAYPYLLLYESRQRRYLGSSPEGSGFCSKDRFVAYPCAVGQTAFSSGR
  HYWEVGMNITGDALWALGVCRDNVSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGI
  FLDFEAGEVSFYSVSDGSHLHTYSQATFPGPLQPFFCLGAPKSGQMVISTVTMWVKG
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016102
• ORF Size: 1431 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016102.4
• RefSeq Size: 1851 bp
• RefSeq ORF: 1434 bp
• Locus ID: 51127
• UniProt ID: Q9Y577
• Cytogenetics: 1q42.13
• Protein Families: Druggable Genome
• MW: 54.2 kDa
• Gene Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein is expressed almost exclusively in the testis, but its function is unknown. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]