SLC12A9 (NM_020246) Human Tagged ORF Clone

CAT#: RC213861

SLC12A9 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 9 (SLC12A9)

Product Images

Circular map for RC213861

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC12A9
• Synonyms: CCC6; CIP1; hCCC6; WO3.3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213861 representing NM_020246
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCGAGAGCTCACCTCTGCTGGCCTACCGGCTCCTGGGGGAGGAGGGGGTTGCCCTCCCTGCCA
  ATGGGGCCGGGGGTCCTGGAGGGGCGTCTGCCCGGAAGCTGTCCACCTTCCTGGGTGTGGTGGTGCCCAC
  TGTCCTGTCCATGTTCAGCATAGTTGTTTTTCTGAGGATTGGGTTCGTGGTGGGTCATGCTGGGCTACTG
  CAGGCCCTGGCCATGCTGCTGGTTGCCTACTTCATCCTGGCACTCACCGTCCTCTCTGTCTGTGCCATCG
  CCACCAATGGAGCCGTGCAGGGGGGCGGAGCCTACTTCATGATCAGCCGCACACTGGGGCCCGAGGTCGG
  GGGCAGCATTGGGCTCATGTTCTACCTGGCTAACGTCTGTGGCTGTGCCGTCTCCCTCCTGGGGCTGGTG
  GAGTCTGTGCTTGATGTCTTCGGGGCCGATGCCACAGGGCCCAGTGGGCTCCGGGTCCTGCCCCAGGGCT
  ACGGCTGGAACCTGCTGTATGGCTCCCTGCTGCTGGGCCTTGTGGGTGGGGTCTGCACCCTGGGAGCCGG
  CCTCTATGCCCGGGCCTCATTCCTCACATTCCTGCTGGTCTCTGGCTCCCTGGCCTCTGTGCTCATCAGT
  TTTGTGGCTGTGGGGCCGAGGGACATCCGCTTGACTCCTAGGCCTGGCCCCAATGGCTCCTCCCTGCCGC
  CCCGGTTTGGCCACTTCACCGGCTTCAACAGCAGTACCCTGAAGGACAACTTGGGCGCTGGCTATGCTGA
  GGACTACACCACGGGAGCCGTGATGAATTTTGCCAGCGTCTTTGCTGTCCTCTTTAACGGCTGTACAGGC
  ATCATGGCTGGGGCCAACATGTCAGGGGAGCTGAAGGACCCCAGCCGGGCGATCCCTCTGGGCACGATCG
  TCGCCGTCGCCTACACCTTCTTCGTCTATGTCCTGCTTTTCTTTCTCTCCAGCTTCACTTGTGACAGGAC
  CCTGCTGCAGGAAGACTATGGGTTCTTCCGCGCCATCAGCCTGTGGCCCCCACTGGTGTTGATCGGAATC
  TATGCCACAGCGCTCTCAGCGTCCATGAGCTCGCTCATTGGTGCCTCCCGCATCCTCCATGCCCTGGCCC
  GGGATGACCTCTTTGGCGTGATCTTGGCACCGGCCAAGGTTGTGTCCCGAGGGGGAAACCCCTGGGCAGC
  TGTACTTTATTCTTGGGGCCTGGTGCAGCTGGTGCTCCTGGCTGGGAAGCTGAACACACTGGCTGCTGTG
  GTCACTGTCTTCTACCTGGTGGCCTATGCTGCCGTGGACCTGTCCTGCCTGAGCCTGGAGTGGGCCTCGG
  CCCCCAACTTCCGCCCCACCTTCAGCCTGTTCTCCTGGCACACCTGCCTGCTGGGGGTGGCCTCCTGCCT
  GCTCATGATGTTCCTCATCAGTCCTGGCGCGGCTGGTGGCTCCCTGCTCCTCATGGGTCTGCTGGCTGCC
  CTGCTCACCGCGCGAGGAGGCCCCAGTAGCTGGGGCTATGTCAGCCAGGCCTTGCTTTTCCACCAGGTGC
  GTAAGTATCTGCTTCGGCTGGACGTCCGGAAGGATCACGTGAAGTTCTGGCGGCCCCAGCTGCTGCTCCT
  GGTGGGGAACCCCCGGGGCGCCCTGCCTCTGCTGCGGTTGGCCAACCAGCTTAAGAAGGGGGGGCTGTAT
  GTGCTGGGCCACGTCACCCTGGGAGACCTCGACTCCCTGCCCTCGGACCCTGTACAGCCGCAGTATGGGG
  CATGGCTCAGCCTGGTGGACCGTGCCCAGGTGAAGGCTTTTGTGGATCTAACCCTCTCACCCTCCGTGCG
  CCAGGGGGCTCAGCATCTGCTGCGAATCTCCGGCCTCGGTGGCATGAAGCCCAACACGTTGGTCCTAGGT
  TTCTACGATGACGCTCCACCGCAGGACCATTTCCTGACGGACCCGGCTTTCTCTGAGCCTGCAGACAGCA
  CCAGGGAGGGCAGTTCCCCAGCTCTGAGCACCCTGTTCCCTCCTCCCCGGGCTCCTGGGAGCCCCCGGGC
  CCTCAATCCCCAGGACTATGTGGCCACGGTGGCCGACGCCCTCAAGATGAACAAGAATGTGGTGCTGGCC
  CGGGCCAGCGGGGCCTTGCCCCCTGAGCGGCTGAGCCGGGGGTCTGGGGGCACCTCTCAGCTGCACCATG
  TGGACGTGTGGCCCCTCAACCTGCTGCGGCCCCGGGGTGGGCCCGGCTATGTGGATGTCTGCGGCCTCTT
  CCTGCTGCAGATGGCAACCATCTTGGGCATGGTGCCCGCTTGGCATAGCGCCCGGCTCCGGATCTTCCTG
  TGCCTGGGGCCTCGGGAGGCGCCTGGGGCGGCCGAGGGGCGGCTGCGGGCACTGCTGAGCCAACTGAGGA
  TCCGGGCTGAGGTGCAGGAGGTGGTGTGGGGCGAGGGGGCCGGGGCTGGGGAACCCGAGGCGGAGGAGGA
  AGGGGACTTTGTGAACAGTGGGCGGGGAGACGCAGAGGCAGAGGCCCTGGCACGCAGCGCCAACGCCCTG
  GTTCGGGCCCAGCAGGGGCGCGGCACAGGAGGAGGGCCGGGTGGGCCGGAGGGTGGGGATGCTGAGGGCC
  CCATCACAGCCCTCACCTTCCTGTACTTGCCTCGGCCGCCAGCCGATCCCGCCCGATACCCCCGCTACCT
  GGCGCTACTGGAGACTCTAACCCGAGACCTGGGCCCCACGCTGCTGGTTCATGGGGTCACTCCAGTCACC
  TGCACTGATCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213861 representing NM_020246
  Red=Cloning site Green=Tags(s)
  
  MASESSPLLAYRLLGEEGVALPANGAGGPGGASARKLSTFLGVVVPTVLSMFSIVVFLRIGFVVGHAGLL
  QALAMLLVAYFILALTVLSVCAIATNGAVQGGGAYFMISRTLGPEVGGSIGLMFYLANVCGCAVSLLGLV
  ESVLDVFGADATGPSGLRVLPQGYGWNLLYGSLLLGLVGGVCTLGAGLYARASFLTFLLVSGSLASVLIS
  FVAVGPRDIRLTPRPGPNGSSLPPRFGHFTGFNSSTLKDNLGAGYAEDYTTGAVMNFASVFAVLFNGCTG
  IMAGANMSGELKDPSRAIPLGTIVAVAYTFFVYVLLFFLSSFTCDRTLLQEDYGFFRAISLWPPLVLIGI
  YATALSASMSSLIGASRILHALARDDLFGVILAPAKVVSRGGNPWAAVLYSWGLVQLVLLAGKLNTLAAV
  VTVFYLVAYAAVDLSCLSLEWASAPNFRPTFSLFSWHTCLLGVASCLLMMFLISPGAAGGSLLLMGLLAA
  LLTARGGPSSWGYVSQALLFHQVRKYLLRLDVRKDHVKFWRPQLLLLVGNPRGALPLLRLANQLKKGGLY
  VLGHVTLGDLDSLPSDPVQPQYGAWLSLVDRAQVKAFVDLTLSPSVRQGAQHLLRISGLGGMKPNTLVLG
  FYDDAPPQDHFLTDPAFSEPADSTREGSSPALSTLFPPPRAPGSPRALNPQDYVATVADALKMNKNVVLA
  RASGALPPERLSRGSGGTSQLHHVDVWPLNLLRPRGGPGYVDVCGLFLLQMATILGMVPAWHSARLRIFL
  CLGPREAPGAAEGRLRALLSQLRIRAEVQEVVWGEGAGAGEPEAEEEGDFVNSGRGDAEAEALARSANAL
  VRAQQGRGTGGGPGGPEGGDAEGPITALTFLYLPRPPADPARYPRYLALLETLTRDLGPTLLVHGVTPVT
  CTDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020246
• ORF Size: 2742 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020246.4
• RefSeq Size: 3322 bp
• RefSeq ORF: 2745 bp
• Locus ID: 56996
• UniProt ID: Q9BXP2
• Cytogenetics: 7q22.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 96.6 kDa
• Gene Summary: May be an inhibitor of SLC12A1. Seems to correspond to a subunit of a multimeric transport system and thus, additional subunits may be required for its function.[UniProtKB/Swiss-Prot Function]