ATP2B4 (NM_001684) Human Tagged ORF Clone

CAT#: RC213835

ATP2B4 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 4 (ATP2B4), transcript variant 2

Product Images

Circular map for RC213835

• 

  Western blot validation of overexpression lysate (Cat# LY419808) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213835 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ATP2B4 protein (Cat# TP313835). The protein was produced from HEK293T cells transfected with ATP2B4 cDNA clone (Cat# RC213835) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATP2B4
• Synonyms: ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213835 representing NM_001684
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGAACCCATCAGACCGTGTCTTGCCTGCCAACTCGATGGCCGAGAGCCGTGAAGGGGACTTTGGCT
  GCACAGTAATGGAACTGAGGAAGCTCATGGAGCTGCGTTCGAGGGATGCACTGACCCAGATTAATGTCCA
  CTATGGAGGTGTACAGAATCTCTGCAGTAGACTGAAAACCTCCCCTGTGGAAGGTCTGTCTGGGAACCCT
  GCAGATCTGGAGAAACGTAGGCAGGTGTTTGGACACAACGTGATCCCCCCCAAAAAGCCCAAGACTTTCT
  TAGAATTAGTGTGGGAAGCTCTTCAAGATGTCACGCTCATCATCCTGGAGATTGCAGCCATCATCTCCCT
  GGTCCTGTCCTTTTATCGCCCTGCTGGTGAAGAAAATGAACTGTGTGGTCAAGTCGCAACTACCCCAGAA
  GATGAAAATGAGGCACAAGCTGGCTGGATTGAGGGGGCAGCCATCCTTTTCTCAGTGATCATCGTGGTGT
  TAGTGACTGCCTTTAATGATTGGAGCAAAGAGAAGCAATTCCGGGGGCTGCAGTGCCGCATTGAACAGGA
  GCAAAAGTTCTCCATCATCCGAAACGGTCAACTCATCCAGCTCCCTGTGGCTGAGATTGTGGTTGGTGAT
  ATTGCCCAAGTCAAATACGGTGATCTGCTGCCTGCAGATGGAATCCTGATCCAAGGAAATGATCTGAAGA
  TTGATGAGAGCTCTCTGACAGGGGAATCTGACCATGTCAAGAAGTCCCTGGACAAAGACCCCATGTTGCT
  CTCAGGGACCCATGTCATGGAAGGTTCTGGCCGGATGGTGGTGACAGCTGTTGGTGTCAACTCTCAGACT
  GGAATCATCCTTACTCTCTTGGGGGTCAATGAGGATGACGAAGGGGAGAAAAAGAAGAAAGGTAAAAAAC
  AAGGAGTCCCTGAAAATCGCAACAAAGCAAAGACCCAAGACGGAGTGGCCCTGGAAATCCAGCCACTCAA
  CAGCCAGGAGGGAATCGACAATGAGGAAAAGGACAAGAAGGCAGTCAAGGTGCCTAAAAAGGAGAAGTCA
  GTGCTGCAGGGCAAGCTGACTCGCCTGGCTGTTCAGATTGGGAAAGCCGGTCTGCTCATGTCTGCTCTCA
  CGGTTTTCATCCTGATTCTATACTTTGTGATTGACAACTTTGTGATAAATCGCAGACCATGGCTCCCTGA
  GTGTACTCCCATCTACATCCAGTACTTTGTCAAGTTCTTCATCATCGGCATCACTGTACTGGTGGTGGCT
  GTGCCAGAGGGGCTGCCTCTGGCTGTCACCATCTCACTGGCCTACTCTGTGAAGAAAATGATGAAAGACA
  ATAACCTAGTACGGCACTTGGATGCTTGTGAGACCATGGGCAACGCCACCGCCATCTGCTCTGATAAGAC
  AGGCACGTTGACCATGAACCGCATGACTGTGGTACAAGCTTATATTGGGGGCATCCATTACCGTCAAATC
  CCAAGCCCTGATGTCTTCCTGCCCAAAGTCCTGGACCTCATTGTCAATGGCATTTCTATCAACAGTGCTT
  ATACCTCCAAGATTCTGCCTCCAGAGAAGGAGGGAGGCCTGCCTCGGCAGGTGGGCAACAAGACCGAGTG
  TGCTCTGCTAGGCTTTGTCACAGATCTGAAGCAGGATTATCAGGCTGTGCGTAATGAAGTGCCCGAGGAG
  AAGCTCTACAAGGTGTACACCTTTAACTCAGTGCGCAAGTCAATGAGCACCGTCATCAGGAATCCCAACG
  GTGGCTTCCGTATGTACAGCAAGGGCGCCTCTGAGATCATCTTGCGCAAGTGTAATCGAATCCTGGACCG
  GAAAGGGGAAGCAGTGCCATTCAAGAATAAAGACAGAGATGATATGGTACGCACTGTCATCGAGCCCATG
  GCCTGTGATGGACTCCGGACTATCTGCATAGCTTACCGGGACTTCGATGACACAGAGCCCTCTTGGGACA
  ATGAGAATGAGATCCTCACCGAACTGACCTGTATCGCGGTGGTGGGCATTGAGGACCCTGTGCGCCCAGA
  GGTGCCAGATGCTATTGCCAAATGCAAACAAGCTGGCATTACTGTCAGAATGGTGACAGGTGACAACATC
  AACACAGCCCGGGCCATTGCCACCAAATGTGGCATTCTGACACCTGGGGATGACTTCCTGTGCTTAGAAG
  GCAAAGAATTCAACCGGCTCATCCGCAACGAGAAAGGCGAGGTAGAGCAAGAAAAGCTGGACAAGATCTG
  GCCTAAGCTTCGGGTCCTGGCGCGATCTTCTCCCACTGACAAGCACACCCTGGTGAAAGGCATAATTGAC
  AGCACTGTTGGGGAACACCGGCAGGTCGTGGCTGTCACTGGTGATGGCACAAATGACGGGCCTGCTCTGA
  AGAAAGCGGATGTTGGTTTTGCCATGGGCATCGCAGGCACAGATGTAGCAAAGGAGGCTTCAGACATCAT
  CCTAACAGATGACAACTTCACCAGCATTGTGAAGGCAGTGATGTGGGGACGAAATGTCTATGACAGCATC
  TCCAAGTTCCTGCAGTTCCAGCTCACTGTCAATGTGGTGGCCGTGATTGTAGCCTTCACTGGAGCCTGTA
  TCACTCAGGATTCCCCATTGAAAGCTGTGCAGATGTTGTGGGTTAATCTGATCATGGACACTTTTGCTTC
  ATTGGCCCTGGCCACAGAGCCCCCTACGGAATCTCTGTTGAAGCGGCGCCCCTATGGCCGAAATAAGCCT
  CTGATCTCACGCACTATGATGAAGAACATCTTGGGCCATGCATTCTATCAGCTCATTGTCATCTTTATCC
  TTGTCTTTGCGGGTGAGAAATTCTTTGATATTGATAGTGGGAGGAAGGCACCTCTACATTCACCACCCAG
  CCAGCACTATACCATTGTTTTTAACACCTTCGTGCTGATGCAGCTCTTCAATGAAATCAACTCCCGAAAG
  ATCCATGGAGAGAAGAACGTCTTTTCAGGCATCTACCGCAACATTATCTTCTGCTCTGTAGTCTTGGGCA
  CATTCATCTGCCAGATTTTCATCGTGGAATTTGGGGGTAAACCCTTCAGTTGTACAAGCCTCAGCCTGTC
  TCAGTGGCTGTGGTGTCTCTTCATTGGGATTGGAGAACTTCTGTGGGGCCAGTTCATCTCCGCAATACCT
  ACCCGATCCCTGAAGTTCCTGAAGGAGGCTGGGCATGGCACCACCAAAGAGGAGATCACCAAGGATGCCG
  AGGGACTGGATGAGATTGACCATGCTGAGATGGAGCTGCGCCGAGGCCAGATCCTCTGGTTCCGGGGCCT
  GAACCGTATCCAGACTCAGATCAAAGTGGTCAAAGCGTTCCATAGTTCCCTCCACGAAAGCATTCAGAAA
  CCCTACAACCAAAAGTCCATCCACAGCTTCATGACCCACCCTGAATTCGCCATAGAGGAGGAGTTGCCAC
  GAACACCACTCCTGGATGAGGAAGAGGAGGAAAATCCTGACAAGGCTTCTAAGTTTGGGACTAGGGTGCT
  CCTGTTGGATGGTGAGGTCACTCCATATGCCAATACAAACAACAATGCGGTGGATTGCAACCAAGTGCAG
  CTCCCCCAGTCGGACAGCTCTCTACAGAGCCTAGAGACATCAGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213835 representing NM_001684
  Red=Cloning site Green=Tags(s)
  
  MTNPSDRVLPANSMAESREGDFGCTVMELRKLMELRSRDALTQINVHYGGVQNLCSRLKTSPVEGLSGNP
  ADLEKRRQVFGHNVIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPAGEENELCGQVATTPE
  DENEAQAGWIEGAAILFSVIIVVLVTAFNDWSKEKQFRGLQCRIEQEQKFSIIRNGQLIQLPVAEIVVGD
  IAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPMLLSGTHVMEGSGRMVVTAVGVNSQT
  GIILTLLGVNEDDEGEKKKKGKKQGVPENRNKAKTQDGVALEIQPLNSQEGIDNEEKDKKAVKVPKKEKS
  VLQGKLTRLAVQIGKAGLLMSALTVFILILYFVIDNFVINRRPWLPECTPIYIQYFVKFFIIGITVLVVA
  VPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGIHYRQI
  PSPDVFLPKVLDLIVNGISINSAYTSKILPPEKEGGLPRQVGNKTECALLGFVTDLKQDYQAVRNEVPEE
  KLYKVYTFNSVRKSMSTVIRNPNGGFRMYSKGASEIILRKCNRILDRKGEAVPFKNKDRDDMVRTVIEPM
  ACDGLRTICIAYRDFDDTEPSWDNENEILTELTCIAVVGIEDPVRPEVPDAIAKCKQAGITVRMVTGDNI
  NTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKIWPKLRVLARSSPTDKHTLVKGIID
  STVGEHRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSI
  SKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLKRRPYGRNKP
  LISRTMMKNILGHAFYQLIVIFILVFAGEKFFDIDSGRKAPLHSPPSQHYTIVFNTFVLMQLFNEINSRK
  IHGEKNVFSGIYRNIIFCSVVLGTFICQIFIVEFGGKPFSCTSLSLSQWLWCLFIGIGELLWGQFISAIP
  TRSLKFLKEAGHGTTKEEITKDAEGLDEIDHAEMELRRGQILWFRGLNRIQTQIKVVKAFHSSLHESIQK
  PYNQKSIHSFMTHPEFAIEEELPRTPLLDEEEEENPDKASKFGTRVLLLDGEVTPYANTNNNAVDCNQVQ
  LPQSDSSLQSLETSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001684
• ORF Size: 3615 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001684.5
• RefSeq Size: 8733 bp
• RefSeq ORF: 3618 bp
• Locus ID: 493
• UniProt ID: P23634
• Cytogenetics: 1q32.1
• Domains: E1-E2_ATPase, Cation_ATPase_N, Hydrolase, Cation_ATPase_C
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway
• MW: 133.8 kDa
• Gene Summary: The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]