PHF16 (JADE3) (NM_001077445) Human Tagged ORF Clone

CAT#: RC213779

PHF16 (Myc-DDK-tagged)-Human PHD finger protein 16 (PHF16), transcript variant 2

Product Images

Circular map for RC213779

• 

  Western blot validation of overexpression lysate (Cat# LY415071) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219274 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PHF16
• Synonyms: JADE-3; PHF16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213779 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACGCCATAGGCCTGTCAGCAGCAGTGACAGTTCAGACGAAAGTCCTTCCACTTCCTTTACTTCTG
  GCTCAATGTATAGGATCAAGTCAAAAATTCCAAATGAACACAAGAAACCTGCTGAGGTATTCCGGAAGGA
  CCTCATCAGTGCCATGAAACTTCCAGATTCTCACCACATTAATCCTGATAGCTATTACCTCTTTGCTGAT
  ACATGGAAGGAAGAATGGGAAAAGGGAGTCCAGGTACCAGCCAGTCCAGACACCGTTCCACAGCCTTCTC
  TCAGGATTATAGCTGAGAAGGTAAAGGACGTTCTGTTTATCCGACCCCGGAAGTATATTCACTGCTCCAG
  CCCAGACACCACAGAGCCTGGCTACATCAACATCATGGAGTTGGCAGCATCTGTTTGCCGCTATGACCTA
  GATGACATGGACATCTTCTGGCTTCAGGAACTCAATGAAGACCTTGCAGAAATGGGTTGTGGGCCAGTTG
  ATGAGAATCTTATGGAAAAGACAGTAGAAGTCCTGGAACGCCATTGCCATGAAAATATGAACCATGCTAT
  TGAGACAGAGGAAGGGCTAGGCATAGAGTATGATGAAGATGTGATCTGTGATGTGTGCCGGTCTCCAGAC
  AGTGAAGAAGGGAATGATATGGTGTTCTGTGATAAGTGTAACGTCTGTGTGCATCAGGCCTGCTATGGCA
  TCCTCAAGGTCCCAGAAGGCAGCTGGCTGTGTCGCTCCTGTGTCCTGGGCATTTATCCGCAATGTGTGTT
  ATGTCCAAAGAAAGGTGGAGCCCTGAAGACCACCAAGACAGGGACTAAATGGGCTCATGTCAGCTGTGCC
  CTGTGGATCCCAGAGGTCAGCATTGCTTGTCCTGAGAGGATGGAACCGATCACGAAGATCTCCCACATCC
  CACCCAGTCGGTGGGCCTTAGTCTGCAACTTGTGCAAGTTGAAGACGGGGGCTTGTATTCAGTGCTCTAT
  AAAAAGCTGCATCACTGCCTTCCACGTCACCTGTGCCTTTGAGCACGGCCTAGAGATGAAGACCATCCTA
  GATGAGGGAGACGAAGTGAAGTTCAAGTCATATTGCCTCAAGCATAGCCAAAACAGGCAGAAACTTGGAG
  AAGCTGAGTACCCCCACCACAGGGCTAAAGAGCAGAGCCAGGCCAAAAGTGAGAAAACCAGCCTGCGGGC
  ACAGAAGCTTCGGGAGCTGGAGGAGGAGTTCTATTCCTTGGTACGAGTGGAAGATGTGGCCGCAGAGCTG
  GGTATGCCCACGCTAGCTGTGGACTTTATCTATAACTACTGGAAACTGAAGCGGAAAAGTAACTTCAATA
  AGCCATTATTTCCTCCAAAGGAGGATGAAGAAAATGGGCTGGTGCAGCCAAAAGAGGAAAGCATTCACAC
  TCGAATGAGAATGTTTATGCATCTACGCCAGGACCTGGAGAGGGTCCGAAATCTGTGCTATATGATAAGC
  AGACGAGAGAAGCTGAAGCTGTCACACAACAAAATACAGGAACAGATCTTCGGTTTGCAAGTCCAGCTTC
  TTAACCAAGAAATTGATGCAGGGCTTCCTTTGACAAATGCACTTGAAAACTCACTGTTTTACCCACCACC
  AAGAATTACCTTGAAGTTAAAAATGCCCAAATCAACCCCAGAAGACCACAGAAACAGCTCCACAGAAACC
  GATCAGCAGCCCCACTCTCCTGACAGCAGCTCATCTGTTCACAGTATAAGGAACATGCAGGTGCCTCAGG
  AGTCACTAGAAATGAGAACAAAATCGTATCCGAGATACCCACTAGAGAGCAAGAATAACCGTTTGCTGGC
  CAGTCTCAGCCATTCTAGGAGTGAAGCAAAGGAGTCCAGTCCTGCTTGGAGAACCCCGTCCTCGGAGTGC
  TATCATGGGCAGTCACTGGGAAAGCCTCTGGTCCTTCAGGCTGCCCTCCATGGACAGTCTTCCATTGGGA
  ATGGGAAAAGTCAGCCTAACTCCAAGTTTGCCAAATCCAATGGCCTGGAGGGCAGCTGGTCTGGGAATGT
  CACCCAAAAAGACAGCTCGAGTGAGATGTTCTGTGACCAGGAGCCTGTGTTCAGCCCCCACTTGGTCAGT
  CAGGGCAGCTTTAGAAAATCCACTGTAGAACACTTTAGTAGGTCCTTTAAAGAGACCACCAATAGGTGGG
  TGAAGAACACAGAGGACCTCCAGTGCTATGTGAAGCCAACCAAGAATATGAGCCCCAAGGAGCAGTTCTG
  GGGTAGACAGGTTCTCAGGCGGTCTGCAGGGAGAGCTCCATATCAGGAAAATGATGGCTATTGCCCAGAT
  TTGGAGCTGAGTGATTCAGAGGCAGAAAGTGATGGGAATAAAGAAAAAGTCAGGGTAAGGAAAGATAGCT
  CAGACAGGGAAAATCCTCCCCATGACTCTAGACGGGATTGCCATGGTAAAAGCAAGACACATCCCCTTTC
  CCACAGTTCAATGCAAAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213779 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKRHRPVSSSDSSDESPSTSFTSGSMYRIKSKIPNEHKKPAEVFRKDLISAMKLPDSHHINPDSYYLFAD
  TWKEEWEKGVQVPASPDTVPQPSLRIIAEKVKDVLFIRPRKYIHCSSPDTTEPGYINIMELAASVCRYDL
  DDMDIFWLQELNEDLAEMGCGPVDENLMEKTVEVLERHCHENMNHAIETEEGLGIEYDEDVICDVCRSPD
  SEEGNDMVFCDKCNVCVHQACYGILKVPEGSWLCRSCVLGIYPQCVLCPKKGGALKTTKTGTKWAHVSCA
  LWIPEVSIACPERMEPITKISHIPPSRWALVCNLCKLKTGACIQCSIKSCITAFHVTCAFEHGLEMKTIL
  DEGDEVKFKSYCLKHSQNRQKLGEAEYPHHRAKEQSQAKSEKTSLRAQKLRELEEEFYSLVRVEDVAAEL
  GMPTLAVDFIYNYWKLKRKSNFNKPLFPPKEDEENGLVQPKEESIHTRMRMFMHLRQDLERVRNLCYMIS
  RREKLKLSHNKIQEQIFGLQVQLLNQEIDAGLPLTNALENSLFYPPPRITLKLKMPKSTPEDHRNSSTET
  DQQPHSPDSSSSVHSIRNMQVPQESLEMRTKSYPRYPLESKNNRLLASLSHSRSEAKESSPAWRTPSSEC
  YHGQSLGKPLVLQAALHGQSSIGNGKSQPNSKFAKSNGLEGSWSGNVTQKDSSSEMFCDQEPVFSPHLVS
  QGSFRKSTVEHFSRSFKETTNRWVKNTEDLQCYVKPTKNMSPKEQFWGRQVLRRSAGRAPYQENDGYCPD
  LELSDSEAESDGNKEKVRVRKDSSDRENPPHDSRRDCHGKSKTHPLSHSSMQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077445
• ORF Size: 2469 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077445.2, NP_001070913.1
• RefSeq Size: 4713 bp
• RefSeq ORF: 2472 bp
• Locus ID: 9767
• UniProt ID: Q92613
• Cytogenetics: Xp11.3
• Protein Families: Druggable Genome, Transcription Factors
• MW: 93.8 kDa
• Gene Summary: This gene encodes a member of a family of large proteins containing PHD (plant homeo domain)-type zinc fingers. The encoded protein may be associated in a nuclear complex that functions in histone H4 acetylation. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Aug 2013]