NOP2 (NM_001033714) Human Tagged ORF Clone

CAT#: RC213776

NOP2 (Myc-DDK-tagged)-Human NOP2 nucleolar protein homolog (yeast) (NOP2), transcript variant 2

Product Images

Circular map for RC213776

• 

  Western blot validation of overexpression lysate (Cat# LY422416) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213776 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NOP2
• Synonyms: NOL1; NOP120; NSUN1; p120
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213776 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGCAAGTTGGACCCTACGAAGGAGAAGCGGGGGCCAGGCCGAAAGGCCCGGAAGCAGAAGGGTG
  CCGAGACAGAACTCGTCAGATTCTTGCCTGCAGTAAGTGACGAAAATTCCAAGAGGCTGTCTAGTCGTGC
  TCGAAAGAGGGCAGCCAAGAGGAGATTGGGCTCTGTTGAAGCCCCTAAGACAAATAAGTCTCCTGAGGCC
  AAACCATTGCCTGGAAAGCTACCAAAAGGAGCTGTCCAGACAGCTGGTAAGAAGGGACCCCAGTCCCTAT
  TTAATGCTCCTCGAGGCAAGAAGCGCCCAGCACCTGGCAGTGATGAGGAAGAGGAGGAGGAAGACTCTGA
  AGAAGATGGTATGGTGAACCACGGGGACCTCTGGGGCTCCGAGGACGATGCTGATACGGTAGATGACTAT
  GGAGCTGACTCCAACTCTGAGGATGAGGAGGAAGGTGAAGCGTTGCTGCCCATTGAAAGAGCTGCTCGGA
  AGCAGAAGGCCCGGGAAGCTGCTGCTGGGATCCAGTGGAGTGAAGAGGAGACCGAGGACGAGGAGGAAGA
  GAAAGAAGTGACCCCTGAGTCAGGCCCCCCAAAGGTGGAAGAGGCAGATGGGGGCCTGCAGATCAATGTG
  GATGAGGAACCATTTGTGCTGCCCCCTGCTGGGGAGATGGAGCAGGATGCCCAGGCTCCAGACCTGCAAC
  GAGTTCACAAGCGGATCCAGGATATTGTGGGAATTCTGCGTGATTTTGGGGCTCAGCGGGAGGAAGGGCG
  GTCTCGTTCTGAATACCTGAACCGGCTCAAGAAGGATCTGGCCATTTACTACTCCTATGGAGACTTCCTG
  CTTGGCAAGCTCATGGACCTCTTCCCTCTGTCTGAGCTGGTGGAGTTCTTAGAAGCTAATGAGGTGCCTC
  GGCCCGTCACCCTCCGGACCAATACCTTGAAAACCCGACGCCGAGACCTTGCACAGGCTCTAATCAATCG
  TGGGGTTAACCTGGATCCCCTGGGCAAGTGGTCAAAGACTGGACTAGTGGTGTATGATTCTTCTGTGCCC
  ATTGGTGCTACCCCCGAGTACCTGGCTGGGCACTACATGCTGCAGGGAGCCTCCAGCATGTTGCCCGTCA
  TGGCCTTGGCACCCCAGGAACATGAGCGGATCCTGGACATGTGTTGTGCCCCTGGAGGAAAGACCAGCTA
  CATGGCCCAGCTGATGAAGAACACGGGTGTGATCCTTGCCAATGACGCCAATGCTGAGCGGCTCAAGAGT
  GTTGTGGGCAACTTGCATCGGCTGGGAGTCACCAACACCATTATCAGCCACTATGATGGGCGCCAGTTCC
  CCAAGGTGGTGGGGGGCTTTGACCGAGTACTGCTGGATGCTCCCTGCAGTGGCACTGGGGTCATCTCCAA
  GGATCCAGCCGTGAAGACTAACAAGGATGAGAAGGACATCCTGCGCTGTGCTCACCTCCAGAAGGAGTTG
  CTCCTGAGTGCTATTGACTCTGTCAATGCGACCTCCAAGACAGGAGGCTACCTGGTTTACTGCACCTGTT
  CTATCACAGTAGAAGAGAATGAGTGGGTGGTAGACTATGCTCTGAAAAAGAGGAATGTGCGACTGGTGCC
  CACGGGCCTAGACTTTGGCCAGGAAGGTTTTACCCGCTTTCGAGAAAGGCGCTTCCACCCCAGTCTGCGT
  TCTACCCGACGCTTCTACCCTCATACCCACAATATGGATGGGTTCTTCATTGCCAAGTTCAAGAAATTTT
  CCAATTCTATCCCTCAGTCCCAGACAGGAAATTCTGAAACAGCCACACCTACAAATGTAGACTTGCCTCA
  GGTCATCCCCAAGTCTGAGAACAGCAGCCAGCCAGCCAAGAAAGCCAAGGGGGCTGCAAAGACAAAGCAG
  CAGCTGCAGAAACAGCAACATCCCAAGAAGGCCTCCTTCCAGAAGCTGAATGGCATCTCCAAAGGGGCAG
  ACTCAGAATTGTCCACTGTACCTTCTGTCACAAAGACCCAAGCTTCCTCCAGCTTCCAGGATAGCAGTCA
  GCCAGCTGGAAAAGCCGAAGGGATCAGGGAGCCAAAGGTGACTGGGAAGCTAAAGCAACGATCACCTAAA
  TTACAGTCCTCCAAGAAAGTTGCTTTCCTCAGGCAGAATGCCCCTCCCAAGGGCACAGACACACAAACAC
  CGGCTGTGTTATCCCCATCCAAGACTCAGGCCACCCTGAAACCTAAGGACCATCATCAGCCCCTTGGAAG
  GGCCAAGGGGGTTGAGAAGCAGCAGTTGCCAGAGCAGCCTTTTGAGAAAGCTGCCTTCCAGAAACAGAAT
  GATACCCCCAAGGGGCCTCAGCCTCCCACTGTGTCTCCCATCCGTTCCAGCCGCCCCCCACCAGCAAAGA
  GGAAGAAATCTCAGTCCAGGGGCAACAGCCAGCTGCTGCTATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213776 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGRKLDPTKEKRGPGRKARKQKGAETELVRFLPAVSDENSKRLSSRARKRAAKRRLGSVEAPKTNKSPEA
  KPLPGKLPKGAVQTAGKKGPQSLFNAPRGKKRPAPGSDEEEEEEDSEEDGMVNHGDLWGSEDDADTVDDY
  GADSNSEDEEEGEALLPIERAARKQKAREAAAGIQWSEEETEDEEEEKEVTPESGPPKVEEADGGLQINV
  DEEPFVLPPAGEMEQDAQAPDLQRVHKRIQDIVGILRDFGAQREEGRSRSEYLNRLKKDLAIYYSYGDFL
  LGKLMDLFPLSELVEFLEANEVPRPVTLRTNTLKTRRRDLAQALINRGVNLDPLGKWSKTGLVVYDSSVP
  IGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDMCCAPGGKTSYMAQLMKNTGVILANDANAERLKS
  VVGNLHRLGVTNTIISHYDGRQFPKVVGGFDRVLLDAPCSGTGVISKDPAVKTNKDEKDILRCAHLQKEL
  LLSAIDSVNATSKTGGYLVYCTCSITVEENEWVVDYALKKRNVRLVPTGLDFGQEGFTRFRERRFHPSLR
  STRRFYPHTHNMDGFFIAKFKKFSNSIPQSQTGNSETATPTNVDLPQVIPKSENSSQPAKKAKGAAKTKQ
  QLQKQQHPKKASFQKLNGISKGADSELSTVPSVTKTQASSSFQDSSQPAGKAEGIREPKVTGKLKQRSPK
  LQSSKKVAFLRQNAPPKGTDTQTPAVLSPSKTQATLKPKDHHQPLGRAKGVEKQQLPEQPFEKAAFQKQN
  DTPKGPQPPTVSPIRSSRPPPAKRKKSQSRGNSQLLLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033714
• ORF Size: 2424 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033714.3
• RefSeq Size: 2772 bp
• RefSeq ORF: 2427 bp
• Locus ID: 4839
• UniProt ID: P46087
• Cytogenetics: 12p13.31
• MW: 89 kDa
• Gene Summary: Involved in ribosomal large subunit assembly (PubMed:24120868). S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 4447 in 28S rRNA (Probable). May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation (Probable).[UniProtKB/Swiss-Prot Function]