Germany
Japan
United Kingdom
China
Myosin (MYH2) (NM_017534) Human Tagged ORF Clone
CAT#: RC213749
- TrueORF®
MYH2 (Myc-DDK-tagged)-Human myosin, heavy chain 2, skeletal muscle, adult (MYH2), transcript variant 1
ORF Plasmid: tGFP
"NM_017534" in other vectors (2)
Product Images
Frequently bought together (5)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
USD 198.00
Other products for "Myosin"
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | Myosin |
| Synonyms | IBM3; MYH2A; MYHas8; MyHC-2A; MyHC-IIa; MYHSA2; MYPOP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213749 representing NM_017534
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCAGACTCAGAATTGGCTGTTTTTGGGGAGGCTGCTCCTTTCCTCCGAAAGTCTGAAAGGGAGC GCATTGAGGCCCAGAATAGGCCCTTTGATGCCAAAACATCTGTCTTTGTGGCGGAGCCCAAAGAATCCTT TGTCAAAGGGACCATCCAGAGCAGAGAAGGAGGAAAAGTGACGGTGAAGACTGAGGGAGGAGCGACTCTG ACAGTGAAGGATGATCAGGTCTTCCCCATGAACCCTCCCAAATATGACAAGATCGAGGATATGGCCATGA TGACTCATCTGCATGAGCCTGCTGTGCTGTACAACCTCAAAGAACGTTATGCAGCCTGGATGATCTACAC CTATTCAGGTCTCTTCTGTGTCACTGTCAACCCCTACAAGTGGCTGCCTGTGTATAAGCCCGAGGTGGTG ACAGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGACAACGCCTATC AGTTCATGCTGACTGACCGAGAGAATCAGTCAATCCTGATCACTGGAGAATCTGGTGCAGGGAAGACTGT GAACACCAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTTACTGGTGAGAAGAAGAAGGAAGAAATT ACTTCTGGCAAAATACAGGGGACTCTGGAAGATCAAATCATCAGTGCCAACCCCCTACTGGAGGCCTTTG GCAACGCCAAGACCGTGAGGAATGACAACTCCTCTCGCTTTGGTAAATTCATCAGAATCCACTTTGGCAC TACTGGAAAACTGGCATCTGCTGATATTGAAACATATCTGCTAGAGAAGTCTAGAGTTGTTTTCCAGCTT AAGGCTGAGAGAAGTTATCATATTTTTTACCAGATTACATCGAATAAGAAACCAGAACTTATTGAAATGC TTCTGATTACCACGAACCCATATGATTACCCATTTGTCAGTCAAGGGGAGATCAGTGTGGCCAGCATCGA TGATCAGGAAGAACTGATGGCCACAGATAGTGCTATTGATATTTTGGGCTTTACTAATGAAGAAAAGGTC TCCATTTACAAGCTCACGGGGGCTGTGATGCATTATGGGAACCTAAAATTTAAGCAAAAGCAGCGTGAGG AGCAAGCAGAGCCAGATGGCACAGAAGTTGCTGACAAGGCGGCCTACCTCCAGAGTCTGAACTCTGCAGA TCTGCTCAAAGCTCTCTGCTACCCCAGGGTCAAGGTCGGCAATGAGTATGTCACCAAAGGCCAGACTGTA GAACAGGTGTCCAACGCAGTAGGTGCTCTGGCCAAAGCCGTCTACGAGAAGATGTTCCTGTGGATGGTTG CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATCGGGGTCTTGGACATTGCTGG TTTTGAGATTTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAG TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACGTTCA TCGACTTCGGGATGGACCTGGCTGCCTGCATCGAGCTCATCGAGAAGCCTATGGGCATCTTCTCCATCCT GGAAGAGGAGTGCATGTTCCCTAAGGCAACAGACACCTCCTTCAAGAACAAGCTGTATGACCAGCACCTG GGCAAGTCTGCCAACTTCCAGAAGCCCAAGGTGGTCAAAGGCAAGGCCGAGGCCCACTTCGCTCTGATTC ACTATGCTGGTGTTGTGGACTACAACATTACTGGCTGGCTGGAGAAGAACAAGGACCCCCTGAATGAGAC CGTGGTTGGACTGTACCAGAAGTCTGCAATGAAAACTCTAGCTCAGCTCTTCTCTGGGGCTCAAACTGCT GAAGGAGAGGGAGCTGGTGGAGGGGCCAAGAAAGGTGGTAAGAAGAAGGGCTCTTCTTTCCAGACAGTGT CTGCCCTTTTCAGAGAGAATTTGAACAAGCTGATGACCAACCTCAGGAGTACCCATCCTCACTTTGTGAG GTGTATCATCCCCAATGAGACAAAAACTCCTGGTGCCATGGAGCATGAGCTTGTCCTCCACCAGCTGAGG TGTAACGGTGTGCTGGAAGGCATCCGCATCTGTAGGAAAGGATTTCCAAGCAGAATCCTTTATGCAGACT TCAAACAGAGATACAAGGTATTAAATGCAAGTGCAATCCCTGAAGGGCAATTCATTGATAGCAAGAAGGC CTCTGAGAAGCTCCTTGCATCCATCGACATTGACCACACCCAGTATAAATTTGGGCACACCAAGGTCTTT TTCAAAGCTGGTCTTCTGGGGCTCCTAGAGGAGATGCGAGATGACAAGCTGGCCCAGCTGATTACCCGAA CCCAGGCCAGGTGCAGAGGGTTCTTGGCAAGAGTGGAGTACCAGAGGATGGTGGAGAGAAGGGAGGCCAT CTTCTGTATCCAGTACAATATCAGATCCTTCATGAATGTCAAGCACTGGCCCTGGATGAAACTCTTCTTC AAGATCAAGCCTCTGTTGAAGAGTGCAGAAACTGAGAAGGAGATGGCCACCATGAAGGAAGAATTTCAGA AAATTAAAGACGAACTTGCCAAGTCAGAGGCAAAAAGGAAGGAACTGGAAGAAAAGATGGTGACGCTGTT GAAAGAAAAAAATGACTTGCAGCTCCAAGTTCAGGCTGAAGCCGAAGGCTTGGCTGATGCAGAGGAAAGG TGTGACCAGCTAATCAAAACCAAAATCCAGCTAGAAGCCAAAATCAAAGAGGTGACTGAGAGAGCTGAGG ATGAGGAAGAGATCAATGCTGAGCTGACAGCCAAGAAGAGGAAACTGGAGGATGAATGTTCAGAACTCAA GAAAGACATTGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAACATGCCACAGAAAACAAG GTGAAAAACCTCACAGAAGAGATGGCAGGTCTGGATGAAACCATTGCTAAGCTGACCAAGGAGAAGAAGG CTCTCCAGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCTGAC CAAAGCTAAAATCAAACTTGAACAACAAGTGGATGATCTTGAAGGGTCCTTGGAGCAAGAAAAGAAACTT CGCATGGACCTAGAAAGGGCTAAGAGGAAACTTGAGGGTGACTTGAAGTTGGCCCAAGAATCCATAATGG ACATTGAAAATGAGAAACAGCAACTTGATGAAAAGCTCAAAAAGAAAGAGTTTGAAATCAGCAATCTGCA AAGCAAGATTGAAGATGAACAGGCACTTGGCATTCAATTGCAGAAGAAAATTAAAGAATTGCAAGCCCGC ATTGAGGAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCCGGGCCAAAGCAGAGAAGCAGCGCTCTG ACCTCTCCCGGGAGCTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCGGTGGGGCCACTTCAGCCCAGAT TGAGATGAACAAGAAGCGGGAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACCCTACAG CATGAAGCCACAGCGGCCACCCTGAGGAAGAAGCATGCAGATAGTGTGGCCGAGCTTGGGGAGCAGATTG ACAACCTGCAGCGAGTGAAGCAGAAGCTGGAGAAGGAGAAGAGTGAGATGAAGATGGAGATTGATGACCT TGCTAGTAATGTAGAAACGGTCTCCAAAGCCAAGGGAAACCTAGAGAAAATGTGCCGGACTCTAGAGGAC CAACTGAGTGAACTGAAATCAAAGGAAGAGGAGCAGCAGCGGCTGATCAATGACCTGACTGCGCAGAGGG GGCGCCTGCAGACTGAATCTGGTGAGTTTTCACGCCAGCTTGATGAAAAGGAAGCTCTGGTGTCTCAGTT ATCAAGAGGCAAACAAGCCTTTACTCAACAGATTGAAGAATTAAAGAGGCAACTTGAAGAGGAGATAAAA GCCAAGAACGCCCTGGCGCATGCCCTGCAGTCTTCCCGCCACGACTGTGACCTGCTGCGGGAACAGTATG AGGAGGAGCAGGAATCCAAGGCCGAGCTGCAGAGAGCACTGTCCAAGGCCAACACCGAGGTTGCCCAATG GAGGACCAAATACGAGACGGACGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTGGCC CAGCGGCTGCAGGCAGCTGAGGAACATGTAGAAGCTGTGAACGCCAAATGTGCTTCCCTCGAAAAGACGA AGCAGCGGCTGCAGAATGAGGTCGAGGACCTCATGCTTGATGTGGAGAGGACAAATGCCGCCTGTGCCGC CCTTGACAAAAAGCAAAGGAACTTCGATAAGATCCTGGCAGAATGGAAACAGAAATGTGAGGAAACGCAT GCTGAGCTTGAGGCCTCCCAGAAGGAGGCCCGTTCCCTTGGCACTGAGCTGTTCAAGATAAAGAATGCCT ATGAGGAATCTTTGGATCAGCTAGAAACCCTGAAGCGAGAGAACAAAAACTTACAGCAGGAGATTTCTGA CCTCACGGAACAGATTGCAGAAGGAGGGAAACGTATCCATGAACTGGAGAAAATAAAGAAACAAGTGGAA CAAGAAAAGTGTGAACTTCAGGCTGCTTTAGAAGAAGCAGAGGCATCTCTTGAACATGAAGAGGGAAAGA TCCTGCGCATCCAGCTTGAGTTGAACCAAGTCAAGTCTGAGGTTGATAGGAAAATTGCTGAAAAAGATGA GGAAATTGACCAGCTGAAGAGAAACCACATTAGAATCGTGGAGTCCATGCAGAGCACGCTGGATGCTGAG ATCAGGAGTAGGAATGATGCCATTAGGCTCAAGAAGAAGATGGAGGGAGACCTCAATGAAATGGAAATCC AGCTGAACCATGCCAACCGCATGGCTGCTGAGGCCCTGAGGAACTACAGGAACACCCAAGGCATCCTCAA GGATACCCAGATCCACCTGGATGATGCTCTCCGGAGCCAGGAGGACCTGAAGGAACAGCTGGCCATGGTG GAGCGCAGAGCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGCGGGCCACTCTGGAACAGACAGAGAGGA GCAGAAAAATCGCAGAACAGGAGCTCCTGGATGCCAGTGAGCGTGTTCAGCTACTGCACACCCAGAACAC CAGCCTGATCAACACCAAGAAGAAGCTGGAGACAGATATTTCCCAAATGCAAGGAGAGATGGAGGACATT CTCCAGGAAGCCCGCAATGCAGAAGAAAAGGCCAAGAAGGCCATCACTGATGCCGCCATGATGGCTGAGG AGCTGAAGAAGGAGCAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACATGGAGCAGACCGTGAA GGATCTGCAGCTCCGTCTGGATGAGGCTGAGCAGCTGGCCCTGAAGGGTGGGAAGAAGCAGATCCAGAAA CTGGAGGCCAGGGTACGGGAGCTGGAAGGAGAGGTTGAGAGTGAGCAAAAGCGTAATGCTGAGGCTGTCA AAGGTCTGCGCAAACATGAGAGGCGAGTGAAGGAACTCACTTACCAGACGGAAGAAGATAGAAAGAATAT TCTCAGGCTTCAAGATTTGGTAGATAAACTTCAGGCAAAAGTGAAATCTTATAAGAGACAAGCTGAGGAG GCTGAGGAACAATCCAACACCAATCTAGCTAAATTCCGCAAGCTCCAGCATGAGCTGGAGGAGGCCGAGG AACGGGCTGACATTGCTGAGTCCCAGGTGAACAAACTGCGGGTGAAGAGCCGGGAGGTTCACACAAAAGT CATAAGTGAAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213749 representing NM_017534
Red=Cloning site Green=Tags(s) MSSDSELAVFGEAAPFLRKSERERIEAQNRPFDAKTSVFVAEPKESFVKGTIQSREGGKVTVKTEGGATL TVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYKPEVV TAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEI TSGKIQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVVFQL KAERSYHIFYQITSNKKPELIEMLLITTNPYDYPFVSQGEISVASIDDQEELMATDSAIDILGFTNEEKV SIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLQSLNSADLLKALCYPRVKVGNEYVTKGQTV EQVSNAVGALAKAVYEKMFLWMVARINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQ FFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHL GKSANFQKPKVVKGKAEAHFALIHYAGVVDYNITGWLEKNKDPLNETVVGLYQKSAMKTLAQLFSGAQTA EGEGAGGGAKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLR CNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDIDHTQYKFGHTKVF FKAGLLGLLEEMRDDKLAQLITRTQARCRGFLARVEYQRMVERREAIFCIQYNIRSFMNVKHWPWMKLFF KIKPLLKSAETEKEMATMKEEFQKIKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQAEAEGLADAEER CDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENK VKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKL RMDLERAKRKLEGDLKLAQESIMDIENEKQQLDEKLKKKEFEISNLQSKIEDEQALGIQLQKKIKELQAR IEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQ HEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNVETVSKAKGNLEKMCRTLED QLSELKSKEEEQQRLINDLTAQRGRLQTESGEFSRQLDEKEALVSQLSRGKQAFTQQIEELKRQLEEEIK AKNALAHALQSSRHDCDLLREQYEEEQESKAELQRALSKANTEVAQWRTKYETDAIQRTEELEEAKKKLA QRLQAAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERTNAACAALDKKQRNFDKILAEWKQKCEETH AELEASQKEARSLGTELFKIKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVE QEKCELQAALEEAEASLEHEEGKILRIQLELNQVKSEVDRKIAEKDEEIDQLKRNHIRIVESMQSTLDAE IRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQGILKDTQIHLDDALRSQEDLKEQLAMV ERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQMQGEMEDI LQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQLRLDEAEQLALKGGKKQIQK LEARVRELEGEVESEQKRNAEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEE AEEQSNTNLAKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017534 |
| ORF Size | 5823 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017534.6 |
| RefSeq Size | 6062 bp |
| RefSeq ORF | 5826 bp |
| Locus ID | 4620 |
| UniProt ID | Q9UKX2 |
| Cytogenetics | 17p13.1 |
| Protein Pathways | Tight junction, Viral myocarditis |
| MW | 222.9 kDa |
| Gene Summary | Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in inclusion body myopathy-3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
{0} Product Review(s)
0 Product Review(s)
Submit review
Be the first one to submit a review
Product Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.