LIMS2 (NM_017980) Human Tagged ORF Clone

CAT#: RC213746

LIMS2 (Myc-DDK-tagged)-Human LIM and senescent cell antigen-like domains 2 (LIMS2), transcript variant 2

Product Images

Circular map for RC213746

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LIMS2 (Cat# RC213746, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIMS2 (Cat# TA811408)(1:2000). Positive lysates LY413411 (100ug) and LC413411 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY413411) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213746 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LIMS2
• Synonyms: LGMD2W; MDRCMTT; PINCH-2; PINCH2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213746 representing NM_017980
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCGGCTGGGTGCGCTGGCCGCGTCGGGGCTGTACCGGCGGCGCCAGCACCGGCAGAGCCCGC
  CGCCAGCCACGGGCAATATGTCGGACGCCTTGGCCAACGCCGTGTGCCAGCGCTGCCAGGCCCGCTTCTC
  CCCCGCCGAGCGCATTGTCAACAGCAATGGGGAGCTGTACCATGAGCACTGCTTCGTGTGTGCCCAGTGC
  TTCCGGCCCTTCCCCGAGGGGCTCTTCTATGAGTTTGAAGGCCGGAAGTACTGCGAACACGACTTCCAAA
  TGCTGTTTGCTCCGTGCTGTGGATCCTGCGGTGAGTTCATCATTGGCCGCGTCATCAAGGCCATGAACAA
  CAACTGGCACCCGGGCTGCTTCCGCTGCGAGCTGTGTGATGTGGAGCTGGCTGACCTGGGCTTTGTGAAG
  AATGCCGGCAGGCATCTCTGCCGGCCTTGCCACAACCGTGAGAAGGCCAAGGGCCTGGGCAAGTACATCT
  GCCAGCGGTGCCACCTGGTCATCGACGAGCAGCCCCTCATGTTCAGGAGCGACGCCTACCACCCTGACCA
  CTTCAACTGCACCCACTGTGGGAAGGAGCTGACAGCCGAGGCCCGCGAGCTGAAGGGTGAGCTCTACTGC
  CTGCCCTGCCATGACAAGATGGGCGTCCCCATCTGCGGGGCCTGCCGCCGGCCCATCGAGGGCCGAGTGG
  TCAACGCGCTGGGCAAGCAGTGGCACGTGGAGCACTTTGTCTGTGCCAAGTGTGAGAAGCCATTCCTGGG
  GCACCGGCACTATGAGAAGAAGGGCCTGGCCTACTGCGAGACTCACTACAACCAGCTCTTCGGGGACGTC
  TGCTACAACTGCAGCCATGTGATTGAAGGCGATGTGGTGTCGGCCCTCAACAAGGCCTGGTGTGTGAGCT
  GCTTCTCCTGCTCCACCTGCAACAGCAAGCTCACCCTGAAGAACAAGTTTGTGGAGTTCGACATGAAGCC
  CGTGTGTAAGAGGTGCTACGAGAAGTTCCCGCTGGAGCTGAAGAAGCGGCTGAAGAAGCTGTCGGAGCTG
  ACCTCCCGCAAGGCCCAGCCCAAGGCCACAGACCTCAACTCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213746 representing NM_017980
  Red=Cloning site Green=Tags(s)
  
  MAARLGALAASGLYRRRQHRQSPPPATGNMSDALANAVCQRCQARFSPAERIVNSNGELYHEHCFVCAQC
  FRPFPEGLFYEFEGRKYCEHDFQMLFAPCCGSCGEFIIGRVIKAMNNNWHPGCFRCELCDVELADLGFVK
  NAGRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRSDAYHPDHFNCTHCGKELTAEARELKGELYC
  LPCHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDV
  CYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSEL
  TSRKAQPKATDLNSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017980
• ORF Size: 1095 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017980.4, NP_060450.2
• RefSeq Size: 2130 bp
• RefSeq ORF: 1098 bp
• Locus ID: 55679
• UniProt ID: Q7Z4I7
• Cytogenetics: 2q14.3
• Domains: LIM
• MW: 41.3 kDa
• Gene Summary: This gene encodes a member of a small family of focal adhesion proteins which interacts with ILK (integrin-linked kinase), a protein which effects protein-protein interactions with the extraceullar matrix. The encoded protein has five LIM domains, each domain forming two zinc fingers, which permit interactions which regulate cell shape and migration. A pseudogene of this gene is located on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]