SLC39A7 (NM_001077516) Human Tagged ORF Clone

CAT#: RC213722

SLC39A7 (Myc-DDK-tagged)-Human solute carrier family 39 (zinc transporter), member 7 (SLC39A7), transcript variant 2

Product Images

Circular map for RC213722

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  Western blot validation of overexpression lysate (Cat# LY421455) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213722 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SLC39A7 protein (Cat# TP313722). The protein was produced from HEK293T cells transfected with SLC39A7 cDNA clone (Cat# RC213722) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC39A7
• Synonyms: D6S115E; D6S2244E; H2-KE4; HKE4; KE4; RING5; ZIP7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213722 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGAGGCCTGGGGGCCCCCCACTGGGTGGCCGTGGGACTGCTGACCTGGGCGACCTTGGGGCTTC
  TGGTGGCTGGACTCGGGGGTCATGACGACCTGCACGACGATCTGCAAGAGGACTTCCATGGCCACAGCCA
  CAGGCACTCACATGAAGATTTCCACCATGGCCACAGCCATGCCCATGGCCATGGCCACACTCACGAGAGC
  ATCTGGCATGGACATACCCACGATCACGACCATGGACATTCACATGAGGATTTACACCATGGCCATAGCC
  ATGGCTACTCCCATGAGAGCCTCTACCACAGAGGACATGGACATGACCATGAGCATAGCCATGGAGGCTA
  TGGGGAGTCTGGGGCTCCAGGCATCAAGCAGGACCTGGATGCTGTCACTCTCTGGGCTTATGCACTGGGG
  GCCACAGTGCTGATCTCAGCAGCTCCATTTTTTGTCCTCTTCCTTATCCCCGTGGAGTCGAACTCTCCCC
  GGCATCGCTCTCTACTTCAGATCTTGCTCAGTTTTGCTTCCGGTGGGCTCCTGGGAGATGCTTTCCTGCA
  CCTCATTCCTCATGCTCTTGAACCTCATTCTCACCACACTCTGGAGCAACCCGGACATGGACACTCCCAC
  AGTGGCCAGGGCCCCATTCTGTCTGTGGGACTGTGGGTTCTCAGTGGAATTGTTGCCTTTCTTGTCGTGG
  AGAAATTTGTGAGACATGTGAAAGGAGGACATGGTCACAGTCATGGACATGGACACGCTCACAGTCATAC
  ACGTGGAAGTCATGGACATGGAAGACAAGAGCGTTCTACCAAGGAGAAGCAGAGCTCAGAGGAAGAAGAA
  AAGGAAACAAGAGGGGTTCAGAAGAGGCGAGGAGGGAGCACAGTACCCAAAGATGGGCCAGTGAGACCTC
  AGAACGCTGAAGAAGAAAAAAGAGGCTTAGACCTGCGTGTGTCGGGGTACCTGAATCTGGCTGCTGACTT
  GGCACACAACTTCACTGATGGTCTGGCCATTGGGGCTTCCTTTCGAGGGGGCCGGGGACTAGGGATCCTG
  ACCACAATGACTGTCCTGCTACATGAAGTGCCCCACGAGGTCGGAGACTTTGCCATCTTGGTCCAGTCTG
  GCTGCAGCAAAAAGCAGGCGATGCGTCTGCAACTACTGACAGCAGTAGGGGCACTGGCAGGCACAGCCTG
  TGCCCTTCTCACTGAAGGAGGAGCAGTGGGCAGTGAAATTGCAGGTGGTGCAGGTCCTGGCTGGGTCCTG
  CCATTTACTGCAGGTGGCTTTATCTACGTAGCAACAGTGTCTGTGTTGCCCGAGCTGCTGAGGGAGGCAT
  CACCATTGCAATCACTTCTGGAGGTGCTGGGGCTGCTGGGGGGAGTTATCATGATGGTGCTGATTGCCCA
  CCTTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213722 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MARGLGAPHWVAVGLLTWATLGLLVAGLGGHDDLHDDLQEDFHGHSHRHSHEDFHHGHSHAHGHGHTHES
  IWHGHTHDHDHGHSHEDLHHGHSHGYSHESLYHRGHGHDHEHSHGGYGESGAPGIKQDLDAVTLWAYALG
  ATVLISAAPFFVLFLIPVESNSPRHRSLLQILLSFASGGLLGDAFLHLIPHALEPHSHHTLEQPGHGHSH
  SGQGPILSVGLWVLSGIVAFLVVEKFVRHVKGGHGHSHGHGHAHSHTRGSHGHGRQERSTKEKQSSEEEE
  KETRGVQKRRGGSTVPKDGPVRPQNAEEEKRGLDLRVSGYLNLAADLAHNFTDGLAIGASFRGGRGLGIL
  TTMTVLLHEVPHEVGDFAILVQSGCSKKQAMRLQLLTAVGALAGTACALLTEGGAVGSEIAGGAGPGWVL
  PFTAGGFIYVATVSVLPELLREASPLQSLLEVLGLLGGVIMMVLIAHLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077516
• ORF Size: 1407 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077516.2
• RefSeq Size: 2172 bp
• RefSeq ORF: 1410 bp
• Locus ID: 7922
• UniProt ID: Q92504
• Cytogenetics: 6p21.32
• Protein Families: Transmembrane
• MW: 50.1 kDa
• Gene Summary: The protein encoded by this gene transports zinc from the Golgi and endoplasmic reticulum to the cytoplasm. This transport may be important for activation of tyrosine kinases, some of which could be involved in cancer progression. Therefore, modulation of the encoded protein could be useful as a therapeutic agent against cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]