PCDH1 (NM_032420) Human Tagged ORF Clone

CAT#: RC213703

PCDH1 (Myc-DDK-tagged)-Human protocadherin 1 (PCDH1), transcript variant 2

Product Images

Circular map for RC213703

• 

  Western blot validation of overexpression lysate (Cat# LY410117) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213703 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PCDH1 protein (Cat# TP313703). The protein was produced from HEK293T cells transfected with PCDH1 cDNA clone (Cat# RC213703) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCDH1
• Synonyms: PC42; PCDH42
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213703 representing NM_032420
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGCGGGGCGGGCGGCCGGCGCTGCCCGGAGGCGGCCCTCCTGATTCTGGGGCCTCCCAGGATGG
  AGCACCTGAGGCACAGCCCAGGCCCTGGGGGGCAACGGCTACTGCTGCCCTCCATGCTGCTAGCACTGCT
  GCTCCTGCTGGCTCCATCCCCAGGCCACGCCACTCGGGTAGTGTACAAGGTGCCGGAGGAACAGCCACCC
  AACACCCTCATTGGGAGCCTCGCAGCCGACTATGGTTTTCCAGATGTGGGGCACCTGTACAAGCTAGAGG
  TGGGTGCCCCGTACCTTCGCGTGGATGGCAAGACAGGTGACATTTTCACCACCGAGACCTCCATCGACCG
  TGAGGGGCTCCGTGAATGCCAGAACCAGCTCCCTGGTGATCCCTGCATCCTGGAGTTTGAGGTATCTATC
  ACAGACCTCGTGCAGAATGGCAGCCCCCGGCTGCTAGAGGGCCAGATAGAAGTACAAGACATCAATGACA
  ACACACCCAACTTCGCCTCACCAGTCATCACTCTGGCCATCCCTGAGAACACCAACATCGGCTCACTCTT
  CCCCATCCCGCTGGCTTCAGACCGTGATGCTGGTCCCAACGGTGTGGCATCCTATGAGCTGCAGGCTGGG
  CCTGAGGCCCAGGAGCTATTTGGGCTGCAGGTGGCAGAGGACCAGGAGGAGAAGCAACCACAGCTCATTG
  TGATGGGCAACCTGGACCGTGAGCGCTGGGACTCCTATGACCTCACCATCAAGGTGCAGGATGGCGGCAG
  CCCCCCACGCGCCAGCAGTGCCCTGCTGCGTGTCACCGTGCTTGACACCAATGACAACGCCCCCAAGTTT
  GAGCGGCCCTCCTATGAGGCCGAACTATCTGAGAATAGCCCCATAGGCCACTCGGTCATCCAGGTGAAGG
  CCAATGACTCAGACCAAGGTGCCAATGCAGAAATCGAATACACATTCCACCAGGCGCCCGAAGTTGTGAG
  GCGTCTTCTTCGACTGGACAGGAACACTGGACTTATCACTGTTCAGGGCCCGGTGGACCGTGAGGACCTA
  AGCACCCTGCGCTTCTCAGTGCTTGCTAAGGACCGAGGCACCAACCCCAAGAGTGCCCGTGCCCAGGTGG
  TTGTGACCGTGAAGGACATGAATGACAATGCCCCCACCATTGAGATCCGGGGCATAGGGCTAGTGACTCA
  TCAAGATGGGATGGCTAACATCTCAGAGGATGTGGCAGAGGAGACAGCTGTGGCCCTGGTGCAGGTGTCT
  GACCGAGATGAGGGAGAGAATGCAGCTGTCACCTGTGTGGTGGCAGGTGATGTGCCCTTCCAGCTGCGCC
  AGGCCAGTGAGACAGGCAGTGACAGCAAGAAGAAGTATTTCCTGCAGACTACCACCCCGCTAGACTACGA
  GAAGGTCAAAGACTACACCATTGAGATTGTGGCTGTGGACTCTGGCAACCCCCCACTCTCCAGCACTAAC
  TCCCTCAAGGTGCAGGTGGTGGACGTCAATGACAACGCACCTGTCTTCACTCAGAGTGTCACTGAGGTCG
  CCTTCCCGGAAAACAACAAGCCTGGTGAAGTGATTGCTGAGATCACTGCCAGTGATGCTGACTCTGGCTC
  TAATGCTGAGCTGGTTTACTCTCTGGAGCCTGAGCCGGCTGCTAAGGGCCTCTTCACCATCTCACCCGAG
  ACTGGAGAGATCCAGGTGAAGACATCTCTGGATCGGGAACAGCGGGAGAGCTATGAGTTGAAGGTGGTGG
  CAGCTGACCGGGGCAGTCCTAGCCTCCAGGGCACAGCCACTGTCCTTGTCAATGTGCTGGACTGCAATGA
  CAATGACCCCAAATTTATGCTGAGTGGCTACAACTTCTCAGTGATGGAGAACATGCCAGCACTGAGTCCA
  GTGGGCATGGTGACTGTCATTGATGGAGACAAGGGGGAGAATGCCCAGGTGCAGCTCTCAGTGGAGCAGG
  ACAACGGTGACTTTGTTATCCAGAATGGCACAGGCACCATCCTATCCAGCCTGAGCTTTGATCGAGAGCA
  ACAAAGCACCTACACCTTCCAGCTGAAGGCAGTGGATGGTGGCGTCCCACCTCGCTCAGCTTACGTTGGT
  GTCACCATCAATGTGCTGGACGAGAATGACAACGCACCCTATATCACTGCCCCTTCTAACACCTCTCACA
  AGCTGCTGACCCCCCAGACACGTCTTGGTGAGACGGTCAGCCAGGTGGCAGCCGAGGACTTTGACTCTGG
  TGTCAATGCTGAGCTGATCTACAGCATTGCAGGTGGCAACCCTTATGGACTCTTCCAGATTGGGTCACAT
  TCAGGTGCCATCACCCTGGAGAAGGAGATTGAGCGGCGCCACCATGGGCTACACCGCCTGGTGGTGAAGG
  TCAGTGACCGCGGCAAGCCCCCACGCTATGGCACAGCCTTGGTCCATCTTTATGTCAATGAGACTCTGGC
  CAACCGCACGCTGCTGGAGACCCTCCTGGGCCACAGCCTGGACACGCCGCTGGATATTGACATTGCTGGG
  GATCCAGAATATGAGCGCTCCAAGCAGCGTGGCAACATTCTCTTTGGTGTGGTGGCTGGTGTGGTGGCCG
  TGGCCTTGCTCATCGCCCTGGCGGTTCTTGTGCGCTACTGCAGACAGCGGGAGGCCAAAAGTGGTTACCA
  GGCTGGTAAGAAGGAGACCAAGGACCTGTATGCCCCCAAGCCCAGTGGCAAGGCCTCCAAGGGAAACAAA
  AGCAAAGGCAAGAAGAGCAAGTCCCCAAAGCCCGTGAAGCCAGTGGAGGACGAGGATGAGGCCGGGCTGC
  AGAAGTCCCTCAAGTTCAACCTGATGAGCGATGCCCCTGGGGACAGTCCCCGCATCCACCTGCCCCTCAA
  CTACCCACCAGGCAGCCCTGACCTGGGCCGCCACTATCGCTCTAACTCCCCACTGCCTTCCATCCAGCTG
  CAGCCCCAGTCACCCTCAGCCTCCAAGAAGCACCAGGTGGTACAGGACCTGCCACCTGCAAACACATTCG
  TGGGCACCGGGGACACCACGTCCACGGGCTCTGAGCAGTACTCCGACTACAGCTACCGCACCAACCCCCC
  CAAATACCCCAGCAAGCAGTTACCTCACCGCCGCGTCACCTTCTCGGCCACCAGCCAGGCCCAGGAGCTG
  CAGGACCCATCCCAGCACAGTTACTATGACAGTGGCCTGGAGGAGTCTGAGACGCCGTCCAGCAAGTCAT
  CCTCAGGGCCTCGACTCGGTCCCCTGGCCCTGCCTGAGGATCACTATGAGCGCACCACCCCTGATGGCAG
  CATAGGAGAGATGGAGCACCCCGAGAATGACCTTCGCCCTTTGCCTGATGTCGCCATGACAGGCACATGT
  ACCCGGGAGTGCAGTGAGTTTGGCCACTCTGACACATGCTGGATGCCTGGCCAGTCATCTCCCAGCCGCC
  GGACCAAGAGCAGCGCCCTCAAACTCTCCACCTTCGTGCCTTACCAGGACCGAGGAGGGCAGGAGCCTGC
  GGGCGCCGGCAGCCCCAGCCCCCCGGAAGACCGGAACACCAAAACGGCCCCCGTGCGCCTCCTGCCCTCC
  TACAGTGCCTTCTCCCACAGTAGCCATGATTCCTGCAAGGACTCGGCCACCTTGGAGGAAATCCCCCTGA
  CCCAGACCTCGGACTTCCCACCCGCAGCCACACCGGCATCTGCCCAGACGGCCAAGCGCGAGATCTACCT
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213703 representing NM_032420
  Red=Cloning site Green=Tags(s)
  
  MDSGAGGRRCPEAALLILGPPRMEHLRHSPGPGGQRLLLPSMLLALLLLLAPSPGHATRVVYKVPEEQPP
  NTLIGSLAADYGFPDVGHLYKLEVGAPYLRVDGKTGDIFTTETSIDREGLRECQNQLPGDPCILEFEVSI
  TDLVQNGSPRLLEGQIEVQDINDNTPNFASPVITLAIPENTNIGSLFPIPLASDRDAGPNGVASYELQAG
  PEAQELFGLQVAEDQEEKQPQLIVMGNLDRERWDSYDLTIKVQDGGSPPRASSALLRVTVLDTNDNAPKF
  ERPSYEAELSENSPIGHSVIQVKANDSDQGANAEIEYTFHQAPEVVRRLLRLDRNTGLITVQGPVDREDL
  STLRFSVLAKDRGTNPKSARAQVVVTVKDMNDNAPTIEIRGIGLVTHQDGMANISEDVAEETAVALVQVS
  DRDEGENAAVTCVVAGDVPFQLRQASETGSDSKKKYFLQTTTPLDYEKVKDYTIEIVAVDSGNPPLSSTN
  SLKVQVVDVNDNAPVFTQSVTEVAFPENNKPGEVIAEITASDADSGSNAELVYSLEPEPAAKGLFTISPE
  TGEIQVKTSLDREQRESYELKVVAADRGSPSLQGTATVLVNVLDCNDNDPKFMLSGYNFSVMENMPALSP
  VGMVTVIDGDKGENAQVQLSVEQDNGDFVIQNGTGTILSSLSFDREQQSTYTFQLKAVDGGVPPRSAYVG
  VTINVLDENDNAPYITAPSNTSHKLLTPQTRLGETVSQVAAEDFDSGVNAELIYSIAGGNPYGLFQIGSH
  SGAITLEKEIERRHHGLHRLVVKVSDRGKPPRYGTALVHLYVNETLANRTLLETLLGHSLDTPLDIDIAG
  DPEYERSKQRGNILFGVVAGVVAVALLIALAVLVRYCRQREAKSGYQAGKKETKDLYAPKPSGKASKGNK
  SKGKKSKSPKPVKPVEDEDEAGLQKSLKFNLMSDAPGDSPRIHLPLNYPPGSPDLGRHYRSNSPLPSIQL
  QPQSPSASKKHQVVQDLPPANTFVGTGDTTSTGSEQYSDYSYRTNPPKYPSKQLPHRRVTFSATSQAQEL
  QDPSQHSYYDSGLEESETPSSKSSSGPRLGPLALPEDHYERTTPDGSIGEMEHPENDLRPLPDVAMTGTC
  TRECSEFGHSDTCWMPGQSSPSRRTKSSALKLSTFVPYQDRGGQEPAGAGSPSPPEDRNTKTAPVRLLPS
  YSAFSHSSHDSCKDSATLEEIPLTQTSDFPPAATPASAQTAKREIYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032420
• ORF Size: 3711 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032420.5
• RefSeq Size: 4779 bp
• RefSeq ORF: 3714 bp
• Locus ID: 5097
• UniProt ID: Q08174
• Cytogenetics: 5q31.3
• Domains: CA
• Protein Families: Transcription Factors, Transmembrane
• MW: 130.1 kDa
• Gene Summary: This gene belongs to the protocadherin subfamily within the cadherin superfamily. The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a possible role in neuronal development. The protein includes an extracelllular region, containing 7 cadherin-like domains, a transmembrane region and a C-terminal cytoplasmic region. Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene. [provided by RefSeq, Jul 2008]