CREB5 (NM_182898) Human Tagged ORF Clone

CAT#: RC213568

CREB5 (Myc-DDK-tagged)-Human cAMP responsive element binding protein 5 (CREB5), transcript variant 1

Product Images

Circular map for RC213568

• 

  Western blot validation of overexpression lysate (Cat# LY405390) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213568 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CREB5
• Synonyms: CRE-BPA; CREB-5; CREBPA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213568 representing NM_182898
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTTATGAGGAATCCAAGATGAATTTGGAGCAGGAGAGGCCGTTTGTCTGCAGTGCCCCAGGCTGCT
  CCCAGCGCTTCCCAACAGAGGACCATCTGATGATTCATAGGCACAAACATGAAATGACTTTGAAGTTTCC
  TTCAATAAAAACAGACAATATGTTATCAGATCAAACTCCGACCCCAACGAGATTCCTGAAGAACTGCGAG
  GAGGTGGGCCTCTTCAGCGAGCTGGACTGCTCCCTGGAGCACGAGTTCAGGAAGGCTCAGGAAGAGGAGA
  GCAGCAAGCGGAATATCTCGATGCATAATGCAGTTGGTGGGGCCATGACGGGGCCCGGAACTCACCAGCT
  TAGCAGCGCTCGGCTGCCCAACCATGACACCAACGTTGTGATTCAGCAAGCCATGCCGTCGCCTCAGTCC
  AGCTCTGTCATCACTCAGGCACCTTCCACCAACCGCCAGATCGGGCCTGTCCCAGGCTCTCTATCTTCTC
  TGCTACATCTCCACAACAGACAGAGACAGCCCATGCCAGCCTCCATGCCTGGGACCCTGCCCAACCCTAC
  AATGCCAGGATCTTCCGCCGTCTTGATGCCAATGGAGCGACAAATGTCAGTGAACTCCAGCATCATGGGG
  ATGCAAGGTCCAAATCTCAGCAACCCCTGTGCTTCTCCCCAGGTCCAGCCAATGCATTCAGAAGCCAAAA
  TGAGGTTGAAGGCTGCATTGACTCACCACCCTGCTGCCATGTCAAATGGGAACATGAACACCATGGGACA
  CATGATGGAGATGATGGGCTCCCGGCAGGACCAGACGCCACACCATCACATGCACTCGCACCCGCATCAG
  CACCAGACACTGCCACCCCATCACCCTTACCCACACCAGCACCAGCACCCAGCACACCATCCTCACCCTC
  AACCCCATCACCAGCAGAACCATCCACATCACCACTCCCATTCCCACCTTCATGCACACCCAGCACATCA
  CCAGACCTCGCCACATCCGCCCCTGCACACCGGCAACCAAGCACAGGTTTCACCAGCAACACAACAGATG
  CAGCCAACCCAGACAATACAGCCACCCCAGCCCACAGGGGGGCGCCGGCGAAGGGTGGTAGACGAGGATC
  CGGACGAGAGGCGGCGGAAATTTCTGGAACGGAACCGGGCAGCTGCCACCCGCTGCAGACAGAAGAGGAA
  GGTCTGGGTGATGTCATTGGAAAAGAAAGCAGAAGAACTCACCCAGACAAACATGCAGCTTCAGAATGAA
  GTGTCTATGTTGAAAAATGAGGTGGCCCAGCTGAAACAGTTGTTGTTAACACATAAAGACTGCCCAATAA
  CAGCCATGCAGAAAGAATCACAAGGATATCTAAGTCCAGAGAGTAGCCCTCCTGCTAGTCCTGTCCCAGC
  TTGCTCCCAGCAACAAGTCATCCAGCATAATACCATCACTACTTCCTCATCGGTCAGCGAGGTGGTAGGA
  AGCTCCACCCTCAGCCAGCTCACCACTCACAGAACAGACCTGAATCCGATTCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213568 representing NM_182898
  Red=Cloning site Green=Tags(s)
  
  MIYEESKMNLEQERPFVCSAPGCSQRFPTEDHLMIHRHKHEMTLKFPSIKTDNMLSDQTPTPTRFLKNCE
  EVGLFSELDCSLEHEFRKAQEEESSKRNISMHNAVGGAMTGPGTHQLSSARLPNHDTNVVIQQAMPSPQS
  SSVITQAPSTNRQIGPVPGSLSSLLHLHNRQRQPMPASMPGTLPNPTMPGSSAVLMPMERQMSVNSSIMG
  MQGPNLSNPCASPQVQPMHSEAKMRLKAALTHHPAAMSNGNMNTMGHMMEMMGSRQDQTPHHHMHSHPHQ
  HQTLPPHHPYPHQHQHPAHHPHPQPHHQQNHPHHHSHSHLHAHPAHHQTSPHPPLHTGNQAQVSPATQQM
  QPTQTIQPPQPTGGRRRRVVDEDPDERRRKFLERNRAAATRCRQKRKVWVMSLEKKAEELTQTNMQLQNE
  VSMLKNEVAQLKQLLLTHKDCPITAMQKESQGYLSPESSPPASPVPACSQQQVIQHNTITTSSSVSEVVG
  SSTLSQLTTHRTDLNPIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182898
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182898.4
• RefSeq Size: 8532 bp
• RefSeq ORF: 1527 bp
• Locus ID: 9586
• UniProt ID: Q02930
• Cytogenetics: 7p15.1
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Prostate cancer
• MW: 56.7 kDa
• Gene Summary: The product of this gene belongs to the CRE (cAMP response element)-binding protein family. Members of this family contain zinc-finger and bZIP DNA-binding domains. The encoded protein specifically binds to CRE as a homodimer or a heterodimer with c-Jun or CRE-BP1, and functions as a CRE-dependent trans-activator. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]