NLRP9 (NM_176820) Human Tagged ORF Clone

CAT#: RC213515

NLRP9 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 9 (NLRP9)

Product Images

Circular map for RC213515

• 

  Western blot validation of overexpression lysate (Cat# LY406108) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213515 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NLRP9
• Synonyms: CLR19.1; NALP9; NOD6; PAN12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213515 representing NM_176820
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAATCTTTTTTTTCGGATTTTGGCTTGTTGTGGTATCTGAAGGAGCTCAGAAAGGAAGAGTTTT
  GGAAATTTAAGGAGCTCCTCAAACAACCTTTGGAGAAATTTGAACTCAAGCCAATCCCCTGGGCTGAGCT
  GAAGAAGGCCTCCAAAGAAGATGTAGCAAAGCTGCTGGACAAACATTACCCAGGAAAGCAGGCATGGGAG
  GTAACACTGAACCTGTTTCTACAGATCAATAGGAAAGATCTCTGGACAAAGGCTCAGGAAGAGATGAGAA
  ATAAGCTAAACCCATACAGAAAGCATATGAAGGAAACATTTCAACTCATATGGGAGAAGGAAACCTGTCT
  TCACGTCCCTGAGCATTTCTACAAAGAAACCATGAAAAATGAGTATAAAGAATTGAATGACGCATATACT
  GCTGCGGCTAGACGACACACTGTGGTCCTGGAAGGTCCTGATGGAATTGGAAAAACAACCCTTTTAAGAA
  AAGTGATGTTGGACTGGGCAGAGGGAAACTTATGGAAGGACAGGTTCACATTTGTGTTTTTCCTCAATGT
  CTGTGAAATGAACGGTATCGCAGAGACCAGCTTACTGGAGCTCCTCTCTAGGGACTGGCCGGAGTCTTCA
  GAGAAGATCGAAGACATTTTTTCCCAGCCAGAGAGAATTCTGTTCATCATGGATGGCTTTGAGCAACTGA
  AGTTTAACTTACAACTTAAGGCTGACTTGAGCGATGATTGGAGGCAGCGGCAGCCAATGCCAATTATCCT
  GAGCAGTTTGTTGCAAAAAAAGATGCTTCCAGAATCCTCTCTCCTTATTGCATTAGGAAAACTGGCTATG
  CAAAAACACTATTTTATGTTGCGGCATCCAAAACTCATAAAGCTCTTAGGATTCAGTGAATCTGAAAAGA
  AGTCGTATTTCTCCTACTTCTTTGGTGAGAAGAGCAAAGCCCTGAAAGTCTTCAATTTTGTGAGAGATAA
  TGGGCCGCTGTTTATCTTGTGCCATAATCCCTTTACGTGCTGGTTGGTCTGTACTTGTGTGAAACAGAGG
  CTAGAGAGGGGAGAAGACCTTGAAATAAACTCCCAAAACACCACCTATTTATATGCATCCTTTTTAACAA
  CTGTATTCAAAGCAGGAAGTCAGAGTTTTCCACCTAAGGTGAACAGAGCCCGACTAAAAAGCCTGTGTGC
  TTTGGCTGCAGAGGGAATTTGGACATATACATTTGTATTTTCCCATGGGGATCTCCGGAGGAATGGGTTA
  TCTGAGTCTGAGGGCGTGATGTGGGTGGGTATGAGACTCCTCCAAAGGAGAGGGGACTGTTTTGCCTTCA
  TGCATCTGTGTATCCAAGAGTTTTGTGCCGCCATGTTTTATTTGCTCAAACGACCCAAAGACGATCCTAA
  CCCGGCCATTGGAAGCATAACCCAGCTTGTAAGAGCAAGTGTGGTTCAGCCTCAAACCCTCTTGACCCAG
  GTGGGGATATTCATGTTTGGAATTTCAACAGAAGAAATCGTCAGCATGCTGGAGACCTCCTTTGGTTTTC
  CACTGTCAAAAGACCTAAAGCAGGAAATAACCCAATGCCTTGAAAGTTTAAGTCAATGTGAAGCTGATAG
  GGAAGCCATAGCTTTCCAGGAACTATTCATTGGTTTGTTTGAAACTCAGGAAAAAGAATTTGTAACCAAA
  GTGATGAATTTCTTTGAAGAAGTTTTCATTTATATTGGTAACATAGAACATTTGGTAATAGCTTCATTCT
  GCCTGAAGCATTGTCAACATTTAACGACACTTCGCATGTGTGTGGAGAATATCTTTCCAGATGACTCAGG
  ATGCATCTCAGATTACAATGAGAAGCTCGTCTACTGGCGGGAGCTTTGCTCAATGTTCATTACCAACAAG
  AACTTCCAGATTTTAGACATGGAAAATACCAGCCTTGATGATCCCTCCCTGGCGATTCTTTGCAAAGCGC
  TGGCTCAGCCTGTTTGTAAACTCCGAAAACTCATATTTACTTCTGTGTACTTTGGACATGATTCAGAATT
  ATTTAAGGCAGTTCTTCACAACCCTCATCTGAAACTTCTGAGCCTGTACGGCACTAGCCTCTCCCAGTCT
  GACATCAGACACCTGTGTGAGACGCTGAAACATCCAATGTGCAAGATAGAAGAGCTGATACTGGGAAAGT
  GTGACATCTCCAGTGAAGTTTGTGAAGACATCGCCTCCGTCCTGGCCTGCAACAGCAAGCTGAAACACCT
  CTCCTTGGTAGAAAATCCCTTGAGGGACGAAGGAATGACGTTGCTGTGTGAAGCCCTGAAGCACTCACAC
  TGTGCCCTGGAGAGGCTGATGTTGATGTACTGCTGTCTCACCTCTGTCTCCTGTGACTCCATTTCCGAAG
  TCCTCTTGTGCAGTAAGTCCCTGTCCCTCCTCGATCTGGGCTCAAATGCCCTGGAAGATAATGGAGTGGC
  ATCTCTGTGTGCAGCGCTGAAGCACCCAGGCTGCAGCATACGGGAGCTGTGGTTGATGGGCTGTTTCCTT
  ACTTCCGATTCCTGTAAGGACATTGCTGCTGTTCTTATTTGCAATGGGAAACTGAAGACCCTGAAACTTG
  GGCATAATGAAATAGGAGACACTGGTGTCAGACAGTTATGTGCAGCTTTGCAGCATCCTCACTGTAAATT
  AGAGTGTCTCGGGCTGCAAACGTGTCCGATCACCCGTGCCTGCTGCGACGACATCGCCGCAGCACTCATC
  GCCTGCAAAACACTGAGGAGCCTGAACCTCGACTGGATTGCCTTGGATGCTGATGCAGTGGTGGTGCTGT
  GTGAGGCATTGAGCCACCCGGACTGTGCCCTGCAGATGCTGGGGCTGCACAAATCTGGCTTTGATGAAGA
  AACTCAGAAGATCCTGATGTCTGTGGAAGAAAAAATTCCCCATCTGACCATTTCACATGGACCTTGGATT
  GACGAGGAATACAAGATCAGGGGTGTGCTCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213515 representing NM_176820
  Red=Cloning site Green=Tags(s)
  
  MAESFFSDFGLLWYLKELRKEEFWKFKELLKQPLEKFELKPIPWAELKKASKEDVAKLLDKHYPGKQAWE
  VTLNLFLQINRKDLWTKAQEEMRNKLNPYRKHMKETFQLIWEKETCLHVPEHFYKETMKNEYKELNDAYT
  AAARRHTVVLEGPDGIGKTTLLRKVMLDWAEGNLWKDRFTFVFFLNVCEMNGIAETSLLELLSRDWPESS
  EKIEDIFSQPERILFIMDGFEQLKFNLQLKADLSDDWRQRQPMPIILSSLLQKKMLPESSLLIALGKLAM
  QKHYFMLRHPKLIKLLGFSESEKKSYFSYFFGEKSKALKVFNFVRDNGPLFILCHNPFTCWLVCTCVKQR
  LERGEDLEINSQNTTYLYASFLTTVFKAGSQSFPPKVNRARLKSLCALAAEGIWTYTFVFSHGDLRRNGL
  SESEGVMWVGMRLLQRRGDCFAFMHLCIQEFCAAMFYLLKRPKDDPNPAIGSITQLVRASVVQPQTLLTQ
  VGIFMFGISTEEIVSMLETSFGFPLSKDLKQEITQCLESLSQCEADREAIAFQELFIGLFETQEKEFVTK
  VMNFFEEVFIYIGNIEHLVIASFCLKHCQHLTTLRMCVENIFPDDSGCISDYNEKLVYWRELCSMFITNK
  NFQILDMENTSLDDPSLAILCKALAQPVCKLRKLIFTSVYFGHDSELFKAVLHNPHLKLLSLYGTSLSQS
  DIRHLCETLKHPMCKIEELILGKCDISSEVCEDIASVLACNSKLKHLSLVENPLRDEGMTLLCEALKHSH
  CALERLMLMYCCLTSVSCDSISEVLLCSKSLSLLDLGSNALEDNGVASLCAALKHPGCSIRELWLMGCFL
  TSDSCKDIAAVLICNGKLKTLKLGHNEIGDTGVRQLCAALQHPHCKLECLGLQTCPITRACCDDIAAALI
  ACKTLRSLNLDWIALDADAVVVLCEALSHPDCALQMLGLHKSGFDEETQKILMSVEEKIPHLTISHGPWI
  DEEYKIRGVLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176820
• ORF Size: 2973 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176820.2, NP_789790.2
• RefSeq Size: 3484 bp
• RefSeq ORF: 2976 bp
• Locus ID: 338321
• UniProt ID: Q7RTR0
• Cytogenetics: 19q13.42
• MW: 113.1 kDa
• Gene Summary: The protein encoded by this gene belongs to the NALP protein family. Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). This protein may play a regulatory role in the innate immune system as similar family members belong to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5. [provided by RefSeq, Jul 2008]