PNMA8B (NM_020709) Human Tagged ORF Clone

CAT#: RC213508

PNMAL2 (Myc-DDK-tagged)-Human PNMA-like 2 (PNMAL2)

Product Images

Circular map for RC213508

• 

  Coomassie blue staining of purified PNMA8B protein (Cat# TP313508). The protein was produced from HEK293T cells transfected with PNMA8B cDNA clone (Cat# RC213508) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PNMA8B
• Synonyms: PNMAL2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213508 representing NM_020709
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGAGCCTTTTGCAGGACTGGTGCCGGAGCCTGGACGTGGACGCGCACAGGGCCCTGCTGGTCA
  CCGGCATCCCGGAGGGCCTGGAGCAGGCAGACGTCGAAGCCGTCCTGCAGCCGACCCTCCTGCCCCTGGG
  CACGTTCAGGTTGCGACACATGAAGGCTTTGATGAACGAGAAGGCCCAGGCCGCCCTGGTGGAGTTTGTG
  GAGGACGTCAATCACGCTGCCATTCCCAGGGAGATCCCAGGCAAGGATGGGGTCTGGAGGGTTCTGTGGA
  AGGACCGTGCGCAGGACACGAGGGTCCTGAGGCAGATGAGACGCCTGCTGCTGGATGACGGGCCCACGCA
  GGCCGCGGAGGCTGGGACCCCCGGGGAGGCACCCACCCCTCCCGCTTCGGAGACGCAGGCCCAGGATTCT
  GGGGAGGTAACAGGGCAGGCTGGCTCGCTTCTTGGGGCAGCCAGGAACCCAAGGAGGGGCCGTCGGGGTC
  GCAGAAACAGAACCAGACGCAACAGGTTGACCCAGAAGGGCAAGAAGAGAAGCCGAGGAGGACGGCCGTC
  TGCTCCCGCGAGGAGTGAGGCCGAGGACTCTTCCGACGAGAGCCTGGGCATCGTGATCGAGGAGATCGAC
  CAGGGCGACCTGAGCGGAGAAGAGGACCAGAGCGCGCTGTACGCCACGCTGCAGGCCGCTGCCAGGGAGC
  TGGTTAGGCAGTGGGCGCCCTGCAACTCCGAGGGGGAAGAAGACGGTCCCCGCGAGTTCTTGGCTCTGGT
  CACCGTCACCGACAAATCGAAGAAAGAAGAGGCAGAGAAGGAGCCAGCTGGGGCCGAATCCATCCGCTTG
  AACACCAAAGAAGACAAAAATGGTGTCCCCGACTTAGTGGCCCTGCTGGCTGTGAGAGACACCCCGGACG
  AGGAGCCGGTGGACAGCGACACTTCGGAGAGCGACTCGCAGGAAAGTGGGGACCAAGAAACAGAGGAGTT
  GGATAATCCTGAGTTCGTGGCCATTGTGGCCTATACCGACCCGTCGGACCCCTGGGCCCGGGAGGAGATG
  TTGAAAATCGCTTCTGTTATCGAGTCGCTGGGCTGGAGCGACGAGAAAGACAAGCGAGACCCCCTCCGAC
  AGGTCTTGTCCGTCATGTCCAAGGACACTAACGGGACCCGCGTGAAGGTGGAAGAGGCGGGCCGCGAGGT
  GGACGCCGTGGTCCTGCGCAAGGCCGGGGATGACGGGGACCTCCGGGAGTGCATTTCCACCTTGGCGCAG
  CCGGATCTCCCTCCCCAGGCGAAGAAGGCTGGGCGTGGCCTCTTCGGGGGCTGGAGCGAGCACCGTGAGG
  ACGAAGGGGGTCTTCTGGAGCTGGTGGCGCTCCTGGCTGCCCAGGACATGGCGGAGGTGATGAAGGAGGA
  AAAAGAAAACGCCTGGGAAGGCGGGAAGTACAAATACCCCAAAGGCAAACTGGGGGAGGTATTGGCGCTC
  CTGGCCGCCCGGGAGAACATGGGGTCCAACGAGGGGTCGGAGGAGGCTTCGGACGAACAGTCCGAGGAGG
  AGTCGGAGGACACCGAGAGCGAGGCGTCGGAGCCGGAGGACAGGGCATCCAGGAAGCCCCGGGCCAAGAG
  GGCGCGCACGGCCCCCAGGGGCCTGACTCCGGCCGGCGCGCCTCCCACCGCTTCCGGGGCCCGCAAAACC
  CGCGCGGGCGGCCGAGGCCGAGGCCGGGGCGTCACTCCCGAGAAGAAAGCCGGGAGCCGGGGCTCGGCCC
  AGGACGACGCCGCAGGAAGCAGGAAGAAGAAGGGGAGCGCGGGCGCCGGGGCCCATGCCAGGGCAGGCGA
  GGCCAAGGGCCAGGCGCCCACTGGATCCAAGGCCGCGCGCGGGAAGAAGGCCCGTCGGGGCCGGAGGCTG
  CCCCCTAAATGCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213508 representing NM_020709
  Red=Cloning site Green=Tags(s)
  
  MAMSLLQDWCRSLDVDAHRALLVTGIPEGLEQADVEAVLQPTLLPLGTFRLRHMKALMNEKAQAALVEFV
  EDVNHAAIPREIPGKDGVWRVLWKDRAQDTRVLRQMRRLLLDDGPTQAAEAGTPGEAPTPPASETQAQDS
  GEVTGQAGSLLGAARNPRRGRRGRRNRTRRNRLTQKGKKRSRGGRPSAPARSEAEDSSDESLGIVIEEID
  QGDLSGEEDQSALYATLQAAARELVRQWAPCNSEGEEDGPREFLALVTVTDKSKKEEAEKEPAGAESIRL
  NTKEDKNGVPDLVALLAVRDTPDEEPVDSDTSESDSQESGDQETEELDNPEFVAIVAYTDPSDPWAREEM
  LKIASVIESLGWSDEKDKRDPLRQVLSVMSKDTNGTRVKVEEAGREVDAVVLRKAGDDGDLRECISTLAQ
  PDLPPQAKKAGRGLFGGWSEHREDEGGLLELVALLAAQDMAEVMKEEKENAWEGGKYKYPKGKLGEVLAL
  LAARENMGSNEGSEEASDEQSEEESEDTESEASEPEDRASRKPRAKRARTAPRGLTPAGAPPTASGARKT
  RAGGRGRGRGVTPEKKAGSRGSAQDDAAGSRKKKGSAGAGAHARAGEAKGQAPTGSKAARGKKARRGRRL
  PPKCR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020709
• ORF Size: 1905 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020709.3
• RefSeq Size: 4739 bp
• RefSeq ORF: 1908 bp
• Locus ID: 57469
• UniProt ID: Q9ULN7
• Cytogenetics: 19q13.32
• MW: 68.4 kDa