Filensin (BFSP1) (NM_001195) Human Tagged ORF Clone

CAT#: RC213412

BFSP1 (Myc-DDK-tagged)-Human beaded filament structural protein 1, filensin (BFSP1), transcript variant 1

Product Images

Circular map for RC213412

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BFSP1 (Cat# RC213412, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFSP1(Cat# TA804071). Positive lysates LY420073 (100ug) and LC420073 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY420073) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213412 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified BFSP1 protein (Cat# TP313412). The protein was produced from HEK293T cells transfected with BFSP1 cDNA clone (Cat# RC213412) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Filensin
• Synonyms: CP94; CP115; CTRCT33; LIFL-H
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213412 representing NM_001195
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCGGCGCAGCTACGTCTTCCAGACCCGCAAGGAGCAGTACGAGCACGCCGACGAGGCTTCGCGCG
  CCGCCGAGCCCGAGCGCCCGGCCGACGAGGGCTGGGCTGGGGCAACGAGCCTGGCGGCGCTGCAGGGGCT
  CGGCGAGCGCGTGGCCGCCCACGTCCAGCGGGCCCGCGCCCTCGAGCAGCGCCATGCCGGGCTCCGGAGG
  CAGCTGGATGCCTTCCAGCGCCTGGGCGAGCTGGCCGGGCCCGAGGACGCCCTCGCCCGCCAAGTCGAGA
  GCAACCGCCAGCGCGTCCGGGACCTGGAGGCCGAGCGCGCCCGGCTGGAGCGCCAGGGCACCGAGGCGCA
  GCGCGCGCTCGACGAGTTCCGAAGCAAGTATGAAAATGAGTGCGAATGTCAACTCCTGCTAAAAGAAATG
  CTTGAACGGCTTAACAAGGAAGCTGATGAAGCCTTGCTGCATAACCTACGCCTTCAGCTGGAAGCCCAAT
  TTCTGCAAGATGATATCAGTGCGGCAAAGGACAGGCACAAGAAGAATCTTCTGGAAGTTCAGACCTATAT
  CAGCATCCTGCAGCAGATCATCCACACCACTCCTCCAGCATCCATTGTGACGAGTGGGATGAGGGAGGAG
  AAGCTCCTGACGGAGCGGGAGGTGGCCGCCCTGCGGAGTCAGCTGGAGGAGGGCCGGGAGGTGCTCTCCC
  ACCTGCAGGCGCAGAGAGTGGAGCTGCAGGCACAGACAACAACTCTGGAACAAGCTATTAAAAGTGCCCA
  TGAGTGTTATGACGATGAGATTCAGCTTTATAACGAGCAGATTGAGACACTGCGCAAGGAGATTGAGGAG
  ACAGAGCGGGTCCTGGAGAAGTCTTCTTACGACTGCCGGCAGCTGGCGGTCGCCCAGCAAACCCTGAAGA
  ATGAGCTGGACCGGTATCATCGTATCATCGAGATTGAAGGCAACAGGCTGACCTCTGCCTTCATTGAAAC
  TCCCATTCCCCTGTTCACCCAGAGCCATGGAGTCTCTCTCAGCACTGGATCCGGTGGGAAAGATCTTACC
  AGAGCTCTGCAGGATATAACAGCAGCAAAACCAAGACAAAAAGCCCTCCCCAAGAATGTTCCAAGGAGAA
  AAGAGATTATAACAAAAGACAAAACCAACGGAGCTCTGGAAGATGCACCATTAAAAGGTTTGGAAGACAC
  AAAGCTGGTACAGGTGGTACTTAAAGAGGAAAGTGAATCTAAGTTTGAATCAGAAAGTAAAGAAGTAAGT
  CCCCTGACACAAGAAGGGGCTCCAGAGGATGTGCCAGATGGAGGGCAGATAAGCAAAGGCTTTGGGAAAC
  TATACAGGAAGGTCAAGGAGAAAGTGAGAAGCCCCAAAGAGCCTGAGACCCCCACTGAGCTCTACACCAA
  AGAGCGGCACGTGCTGGTCACAGGGGATGCCAATTACGTGGACCCTAGATTCTATGTCTCCTCCATCACA
  GCTAAAGGTGGGGTGGCTGTTTCTGTTGCGGAAGACTCTGTGCTTTATGACGGCCAGGTGGAGCCCTCTC
  CTGAGTCACCCAAGCCCCCTTTAGAGAATGGGCAGGTGGGTCTGCAGGAGAAAGAAGATGGACAACCAAT
  TGACCAGCAGCCTATAGACAAGGAGATTGAGCCAGATGGTGCAGAGCTGGAAGGCCCTGAAGAGAAACGT
  GAGGGTGAGGAGCGGGACGAAGAGTCCAGGAGACCCTGTGCCATGGTCACACCCGGTGCAGAGGAACCAT
  CTATACCTGAGCCTCCAAAGCCTGCGGCTGATCAGGATGGAGCTGAGGTGCTTGGGACTAGGAGCAGAAG
  CCTGCCAGAAAAAGGCCCTCCCAAGGCTTTGGCCTATAAGACAGTGGAAGTGGTGGAATCTATCGAGAAG
  ATTTCCACGGAGAGCATTCAGACATATGAAGAAACCGCTGTGATCGTGGAGACCATGATTGGAAAGACAA
  AGTCAGACAAGAAGAAATCAGGAGAGAAGAGCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213412 representing NM_001195
  Red=Cloning site Green=Tags(s)
  
  MYRRSYVFQTRKEQYEHADEASRAAEPERPADEGWAGATSLAALQGLGERVAAHVQRARALEQRHAGLRR
  QLDAFQRLGELAGPEDALARQVESNRQRVRDLEAERARLERQGTEAQRALDEFRSKYENECECQLLLKEM
  LERLNKEADEALLHNLRLQLEAQFLQDDISAAKDRHKKNLLEVQTYISILQQIIHTTPPASIVTSGMREE
  KLLTEREVAALRSQLEEGREVLSHLQAQRVELQAQTTTLEQAIKSAHECYDDEIQLYNEQIETLRKEIEE
  TERVLEKSSYDCRQLAVAQQTLKNELDRYHRIIEIEGNRLTSAFIETPIPLFTQSHGVSLSTGSGGKDLT
  RALQDITAAKPRQKALPKNVPRRKEIITKDKTNGALEDAPLKGLEDTKLVQVVLKEESESKFESESKEVS
  PLTQEGAPEDVPDGGQISKGFGKLYRKVKEKVRSPKEPETPTELYTKERHVLVTGDANYVDPRFYVSSIT
  AKGGVAVSVAEDSVLYDGQVEPSPESPKPPLENGQVGLQEKEDGQPIDQQPIDKEIEPDGAELEGPEEKR
  EGEERDEESRRPCAMVTPGAEEPSIPEPPKPAADQDGAEVLGTRSRSLPEKGPPKALAYKTVEVVESIEK
  ISTESIQTYEETAVIVETMIGKTKSDKKKSGEKSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195
• ORF Size: 1995 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195.5
• RefSeq Size: 2176 bp
• RefSeq ORF: 1998 bp
• Locus ID: 631
• UniProt ID: Q12934
• Cytogenetics: 20p12.1
• Domains: filament
• MW: 74.4 kDa
• Gene Summary: This gene encodes a lens-specific intermediate filament-like protein named filensin. The encoded protein is expressed in lens fiber cells after differentiation has begun. This protein functions as a component of the beaded filament which is a cytoskeletal structure found in lens fiber cells. Mutations in this gene are the cause of autosomal recessive cortical juvenile-onset cataract. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]