TAF2 (NM_003184) Human Tagged ORF Clone

CAT#: RC213159

TAF2 (Myc-DDK-tagged)-Human TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa (TAF2)

Product Images

Circular map for RC213159

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TAF2 (Cat# RC213159, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TAF2(Cat# TA504588). Positive lysates LY418826 (100ug) and LC418826 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY418826) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213159 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAF2
• Synonyms: CIF150; MRT40; TAF2B; TAFII150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213159 representing NM_003184
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCTGACTGGTGTAGAGCCCGCCAGAATGAACAGGAAGAAAGGAGACAAGGGCTTTGAAAGCCCAA
  GGCCATATAAATTAACCCATCAGGTCGTCTGCATCAACAACATAAATTTCCAGAGAAAATCTGTTGTGGG
  ATTTGTGGAACTGACTATATTTCCCACAGTTGCAAACTTGAATAGAATCAAGTTGAACAGCAAACAGTGT
  AGAATATACCGAGTAAGGATCAATGATTTAGAGGCTGCTTTTATTTATAATGACCCAACCTTGGAAGTTT
  GTCACAGTGAATCAAAACAGAGAAACCTCAATTATTTTTCCAATGCTTATGCAGCTGCAGTTAGTGCTGT
  GGACCCTGATGCAGGAAATGGAGAACTTTGCATTAAGGTTCCATCAGAGCTATGGAAACACGTTGATGAG
  TTAAAGGTCCTGAAGATACACATCAATTTTTCTTTGGATCAGCCCAAAGGAGGTCTTCATTTTGTGGTAC
  CCAGTGTAGAGGGAAGTATGGCAGAGAGAGGTGCTCATGTTTTCTCTTGTGGGTATCAAAATTCTACAAG
  ATTTTGGTTCCCTTGTGTTGATTCATACTCTGAATTGTGTACATGGAAATTAGAATTTACAGTAGATGCT
  GCAATGGTTGCTGTTTCTAATGGCGATTTGGTGGAGACAGTGTATACTCATGATATGAGGAAGAAAACTT
  TCCATTATATGCTTACCATTCCTACAGCAGCGTCAAATATCTCCTTGGCCATTGGACCATTTGAAATACT
  GGTAGATCCATACATGCATGAGGTTACTCATTTTTGTTTGCCCCAACTTCTTCCATTGCTGAAACATACC
  ACATCATACCTTCATGAAGTCTTTGAATTTTATGAAGAAATTCTTACATGTCGTTACCCATACTCCTGTT
  TTAAGACTGTCTTCATTGATGAGGCTTATGTTGAAGTGGCTGCTTATGCTTCCATGAGCATTTTTAGCAC
  AAATCTTTTACACAGTGCCATGATTATAGATGAGACACCTTTGACTAGAAGGTGTTTAGCCCAATCCTTG
  GCCCAGCAGTTTTTTGGTTGTTTCATATCTAGAATGTCTTGGTCTGATGAATGGGTGCTGAAGGGAATTT
  CAGGCTATATCTATGGACTTTGGATGAAAAAAACTTTTGGTGTTAATGAGTACCGCCATTGGATTAAAGA
  GGAGCTAGACAAAATAGTGGCATATGAACTAAAAACTGGTGGGGTTTTACTACATCCCATATTTGGTGGA
  GGAAAAGAGAAGGATAATCCGGCTTCCCATCTACACTTTTCAATAAAGCATCCACATACACTGTCCTGGG
  AATACTACACTATGTTTCAGTGTAAAGCCCACCTTGTGATGAGATTGATTGAAAATAGGATCAGTATGGA
  ATTTATGCTACAAGTTTTCAATAAACTGCTAAGTCTGGCTAGTACTGCTTCATCTCAGAAGTTCCAGTCA
  CATATGTGGAGTCAGATGTTGGTTTCCACATCTGGGTTTTTGAAATCCATTTCAAATGTCTCTGGCAAAG
  ATATTCAGCCGTTAATAAAGCAGTGGGTAGATCAGAGTGGAGTGGTAAAATTTTATGGAAGTTTTGCATT
  TAATAGAAAACGAAATGTCTTGGAACTGGAAATAAAACAGGACTATACATCTCCTGGAACTCAGAAATAC
  GTGGGACCACTTAAAGTGACAGTGCAGGAGTTAGATGGATCCTTCAATCATACACTGCAAATTGAAGAAA
  ACAGCCTTAAACATGATATACCCTGCCATTCCAAAAGTAGAAGGAATAAAAAGAAAAAAATCCCACTGAT
  GAATGGAGAAGAAGTTGATATGGATCTTTCTGCAATGGATGCTGATTCCCCTTTGCTGTGGATAAGGATA
  GACCCAGATATGTCAGTATTGAGGAAGGTAGAATTTGAGCAAGCTGATTTTATGTGGCAGTATCAGCTCC
  GCTATGAGAGAGATGTTGTTGCACAGCAGGAATCCATTTTGGCTTTGGAAAAATTCCCTACTCCAGCATC
  TCGGCTTGCACTCACTGATATATTAGAACAAGAGCAGTGTTTCTACAGAGTAAGAATGTCAGCTTGCTTC
  TGTCTTGCAAAGATTGCAAATTCAATGGTGAGCACATGGACAGGACCACCAGCCATGAAGTCACTCTTCA
  CTAGGATGTTTTGTTGTAAAAGTTGTCCAAACATTGTGAAAACAAACAACTTTATGAGCTTTCAAAGCTA
  TTTTCTACAGAAGACTATGCCAGTTGCAATGGCTTTATTAAGAGATGTTCATAATCTTTGTCCTAAAGAA
  GTCTTAACGTTTATTTTAGACTTAATCAAGTACAATGACAACAGGAAAAATAAGTTTTCAGATAACTATT
  ATCGTGCAGAAATGATTGATGCCCTGGCCAACTCTGTTACACCTGCAGTCAGTGTGAATAATGAAGTTAG
  AACTTTGGATAACTTAAATCCTGATGTGCGACTCATTCTTGAAGAAATCACCAGATTTTTGAATATGGAA
  AAACTTCTTCCGAGTTACAGGCATACCATCACTGTCAGTTGTTTGAGAGCCATACGGGTACTTCAGAAGA
  ACGGACATGTGCCAAGTGATCCAGCTCTTTTTAAATCTTATGCTGAATATGGCCACTTTGTGGACATTAG
  GATAGCAGCTTTGGAAGCAGTTGTTGATTATACTAAAGTGGACAGAAGTTATGAAGAACTGCAATGGCTA
  CTTAATATGATTCAGAATGACCCTGTACCCTATGTAAGGCATAAGATTCTCAACATGTTGACTAAGAACC
  CACCATTTACTAAGAACATGGAGTCTCCCTTATGCAATGAAGCCCTGGTAGATCAACTTTGGAAACTTAT
  GAATTCTGGTACTTCACATGACTGGAGGTTACGGTGTGGTGCTGTGGACTTGTACTTCACACTTTTTGGC
  CTCAGTAGACCTTCCTGTTTACCCTTGCCAGAGCTTGGGTTGGTTCTTAATCTAAAGGAGAAAAAAGCTG
  TCTTGAATCCTACCATAATTCCAGAGTCAGTAGCAGGCAACCAAGAAGCTGCAAATAATCCAAGCAGTCA
  CCCACAGCTAGTTGGATTTCAGAACCCTTTTTCCAGTTCTCAAGATGAGGAGGAGATTGATATGGATACT
  GTTCATGATAGCCAGGCCTTCATTTCCCATCATTTAAACATGCTTGAAAGGCCGTCAACTCCAGGGCTCT
  CGAAATATCGGCCAGCTAGCTCCCGATCTGCTTTAATACCCCAGCACTCAGCAGGCTGTGACAGCACACC
  CACCACAAAACCCCAGTGGAGTTTGGAACTTGCACGGAAGGGAACAGGTAAAGAACAAGCACCTTTGGAG
  ATGAGTATGCATCCAGCGGCAAGCGCTCCACTCTCAGTCTTTACTAAGGAATCTACAGCCTCCAAACACA
  GTGACCACCATCACCACCATCACCATGAGCACAAGAAAAAGAAGAAGAAGCATAAACATAAGCACAAACA
  CAAGCATAAGCATGACAGTAAAGAAAAGGACAAGGAGCCTTTCACTTTCTCCAGCCCTGCCAGTGGCAGG
  TCTATTCGTTCTCCTTCCCTTTCAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213159 representing NM_003184
  Red=Cloning site Green=Tags(s)
  
  MPLTGVEPARMNRKKGDKGFESPRPYKLTHQVVCINNINFQRKSVVGFVELTIFPTVANLNRIKLNSKQC
  RIYRVRINDLEAAFIYNDPTLEVCHSESKQRNLNYFSNAYAAAVSAVDPDAGNGELCIKVPSELWKHVDE
  LKVLKIHINFSLDQPKGGLHFVVPSVEGSMAERGAHVFSCGYQNSTRFWFPCVDSYSELCTWKLEFTVDA
  AMVAVSNGDLVETVYTHDMRKKTFHYMLTIPTAASNISLAIGPFEILVDPYMHEVTHFCLPQLLPLLKHT
  TSYLHEVFEFYEEILTCRYPYSCFKTVFIDEAYVEVAAYASMSIFSTNLLHSAMIIDETPLTRRCLAQSL
  AQQFFGCFISRMSWSDEWVLKGISGYIYGLWMKKTFGVNEYRHWIKEELDKIVAYELKTGGVLLHPIFGG
  GKEKDNPASHLHFSIKHPHTLSWEYYTMFQCKAHLVMRLIENRISMEFMLQVFNKLLSLASTASSQKFQS
  HMWSQMLVSTSGFLKSISNVSGKDIQPLIKQWVDQSGVVKFYGSFAFNRKRNVLELEIKQDYTSPGTQKY
  VGPLKVTVQELDGSFNHTLQIEENSLKHDIPCHSKSRRNKKKKIPLMNGEEVDMDLSAMDADSPLLWIRI
  DPDMSVLRKVEFEQADFMWQYQLRYERDVVAQQESILALEKFPTPASRLALTDILEQEQCFYRVRMSACF
  CLAKIANSMVSTWTGPPAMKSLFTRMFCCKSCPNIVKTNNFMSFQSYFLQKTMPVAMALLRDVHNLCPKE
  VLTFILDLIKYNDNRKNKFSDNYYRAEMIDALANSVTPAVSVNNEVRTLDNLNPDVRLILEEITRFLNME
  KLLPSYRHTITVSCLRAIRVLQKNGHVPSDPALFKSYAEYGHFVDIRIAALEAVVDYTKVDRSYEELQWL
  LNMIQNDPVPYVRHKILNMLTKNPPFTKNMESPLCNEALVDQLWKLMNSGTSHDWRLRCGAVDLYFTLFG
  LSRPSCLPLPELGLVLNLKEKKAVLNPTIIPESVAGNQEAANNPSSHPQLVGFQNPFSSSQDEEEIDMDT
  VHDSQAFISHHLNMLERPSTPGLSKYRPASSRSALIPQHSAGCDSTPTTKPQWSLELARKGTGKEQAPLE
  MSMHPAASAPLSVFTKESTASKHSDHHHHHHHEHKKKKKKHKHKHKHKHKHDSKEKDKEPFTFSSPASGR
  SIRSPSLSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003184
• ORF Size: 3597 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003184.2, NP_003175.1
• RefSeq Size: 5019 bp
• RefSeq ORF: 3600 bp
• Locus ID: 6873
• UniProt ID: Q6P1X5
• Cytogenetics: 8q24.12
• Domains: Peptidase_M1
• Protein Families: Protease, Transcription Factors
• Protein Pathways: Basal transcription factors
• MW: 136.8 kDa
• Gene Summary: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators. [provided by RefSeq, Jul 2008]