ADAMTS19 (NM_133638) Human Tagged ORF Clone

CAT#: RC213075

ADAMTS19 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 19 (ADAMTS19)

Product Images

Circular map for RC213075

• 

  Western blot validation of overexpression lysate (Cat# LY408759) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213075 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADAMTS19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213075 representing NM_133638
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCTGACTCACATCTGCTGCTGCTGCCTCCTTTACCAGCTGGGGTTCCTGTCGAATGGGATCGTTT
  CAGAGCTGCAGTTCGCCCCCGACCGCGAGGAGTGGGAAGTCGTGTTTCCTGCGCTCTGGCGCCGGGAGCC
  GGTGGACCCGGCTGGCGGCAGCGGGGGCAGCGCGGACCCGGGCTGGGTGCGCGGCGTTGGGGGCGGCGGA
  AGCGCCCGGGCGCAGGCTGCCGGCAGCTCACGCGAGGTGCGCTCTGTGGCTCCGGTGCCTTTGGAGGAGC
  CCGTGGAGGGCCGATCAGAGTCCCGGCTCCGGCCCCCGCCGCCGTCGGAGGGTGAGGAGGACGAGGAGCT
  CGAGTCGCAGGAGCTGCCGCGGGGATCCAGCGGGGCTGCCGCCTTGTCCCCGGGCGCCCCGGCCTCGTGG
  CAGCCGCCGCCTCCCCCGCAGCCGCCCCCGTCCCCGCCCCCGGCCCAGCATGCCGAGCCGGATGGCGACG
  AAGTGTTGCTGCGGATCCCGGCCTTCTCTCGGGACCTGTACCTGCTGCTCCGGAGAGACGGCCGCTTCCT
  GGCGCCGCGCTTCGCAGTGGAACAGCGGCCAAATCCCGGCCCCGGCCCCACGGGGGCAGCATCCGCCCCG
  CAACCTCCCGCGCCACCAGACGCAGGCTGCTTCTACACCGGAGCTGTGCTGCGGCACCCTGGCTCGCTGG
  CTTCTTTCAGCACCTGTGGAGGTGGCCTGATGGGATTTATACAGCTCAATGAGGACTTCATATTTATTGA
  GCCACTCAATGATACAATGGCCATAACAGGTCACCCACACCGTGTATATAGGCAGAAAAGGTCCATGGAG
  GAAAAGGTCACAGAGAAGTCAGCTCTTCACAGTCATTACTGTGGTATCATTTCAGATAAAGGAAGACCTA
  GGTCTAGAAAAATAGCAGAAAGTGGAAGAGGGAAACGATATTCATACAAATTACCTCAAGAATACAACAT
  AGAGACTGTAGTGGTTGCAGACCCAGCAATGGTTTCCTATCATGGAGCAGATGCAGCCAGGAGATTCATT
  CTAACCATCTTAAATATGGTATTTAACCTTTTCCAACACAAGAGTCTGAGTGTGCAGGTCAATCTTCGTG
  TGATAAAGCTTATTCTGCTCCATGAAACTCCACCAGAACTATATATTGGGCATCATGGAGAAAAAATGCT
  AGAGAGTTTTTGTAAGTGGCAACATGAAGAATTTGGCAAAAAGAATGATATACATTTAGAGATGTCAACA
  AACTGGGGGGAAGACATGACTTCAGTGGATGCAGCTATACTTATAACAAGGAAAGATTTCTGTGTGCACA
  AAGATGAACCATGTGATACTGTTGGTATAGCTTACTTGAGTGGAATGTGTAGTGAAAAGAGAAAATGTAT
  TATTGCTGAAGACAATGGCTTGAATCTTGCTTTTACAATTGCTCATGAAATGGGTCACAACATGGGCATT
  AACCATGACAATGACCACCCATCGTGTGCTGATGGTCTTCATATCATGTCTGGTGAATGGATTAAAGGAC
  AGAATCTTGGTGACGTTTCATGGTCTCGATGTAGCAAGGAAGATTTGGAAAGATTTCTCAGGTCAAAGGC
  CAGTAACTGCTTGCTACAAACAAATCCGCAGAGTGTCAATTCTGTGATGGTTCCCTCCAAGCTGCCAGGG
  ATGACATACACTGCTGATGAACAATGCCAGATCCTTTTTGGGCCATTGGCTTCTTTTTGTCAGGAGATGC
  AGCATGTTATTTGCACAGGATTATGGTGCAAGGTAGAAGGTGAGAAAGAATGCAGAACCAAGCTAGACCC
  ACCAATGGATGGAACTGACTGTGACCTTGGTAAGTGGTGTAAGGCTGGAGAATGTACCAGCAGGACCTCA
  GCACCTGAACATCTGGCCGGAGAGTGGAGCCTGTGGAGTCCTTGTAGCCGAACCTGCAGTGCTGGGATCA
  GCAGTCGAGAGCGCAAATGTCCTGGGCTAGATTCTGAAGCAAGGGATTGTAATGGTCCCAGAAAACAATA
  CAGAATATGTGAGAATCCACCTTGTCCTGCAGGTTTGCCTGGATTCAGAGACTGGCAATGTCAGGCTTAT
  AGTGTTAGAACTTCCTCCCCAAAGCATATACTTCAGTGGCAAGCTGTCCTGGATGAAGAAAAACCATGTG
  CCTTGTTTTGCTCTCCTGTTGGAAAAGAACAGCCTATTCTTCTATCAGAAAAAGTGATGGATGGAACTTC
  TTGTGGCTATCAGGGATTAGATATCTGTGCAAATGGCAGGTGCCAGAAAGTTGGCTGTGATGGTTTATTA
  GGGTCTCTTGCAAGAGAAGATCATTGTGGTGTATGCAATGGCAATGGAAAATCATGCAAGATCATTAAAG
  GGGATTTTAATCACACCAGAGGAGCAGGTTATGTAGAAGTGCTGGTGATACCTGCTGGAGCAAGAAGAAT
  CAAAGTTGTGGAGGAAAAGCCGGCACATAGCTATTTAGCTCTCCGAGATGCTGGCAAACAGTCTATTAAT
  AGTGACTGGAAGATTGAACACTCTGGAGCCTTCAATTTGGCTGGAACTACCGTTCATTATGTAAGACGAG
  GCCTCTGGGAGAAGATCTCTGCCAAAGGTCCTACTACAGCACCTTTACATCTTCTGGTGCTCCTGTTTCA
  GGATCAGAATTATGGTCTTCACTATGAATACACTATCCCATCAGACCCTCTTCCAGAAAACCAGAGCTCT
  AAAGCACCTGAGCCCCTCTTCATGTGGACACACACAAGCTGGGAAGATTGCGATGCCACTTGTGGAGGAG
  GAGAAAGGAAGACAACAGTGTCCTGCACAAAAATCATGAGCAAAAATATCAGCATTGTGGACAATGAGAA
  ATGCAAATACTTAACCAAGCCAGAGCCACAGATTCGAAAGTGCAATGAGCAACCATGTCAAACAAGGTGG
  ATGATGACAGAATGGACCCCTTGTTCACGAACTTGTGGAAAAGGAATGCAGAGCAGACAAGTGGCCTGTA
  CCCAACAACTGAGCAATGGAACACTGATTAGAGCCCGAGAGAGGGACTGCATTGGGCCCAAGCCCGCCTC
  TGCCCAGCGCTGTGAGGGCCAGGACTGCATGACCGTGTGGGAGGCGGGAGTGTGGTCTGAGTGTTCAGTC
  AAGTGTGGCAAAGGCATACGTCATCGGACCGTTAGATGTACCAACCCAAGAAAGAAGTGTGTCCTCTCTA
  CCAGACCCAGGGAGGCTGAAGACTGTGAGGATTATTCAAAATGCTATGTGTGGCGAATGGGTGACTGGTC
  TAAGTGCTCAATTACCTGTGGCAAAGGAATGCAGTCCCGTGTAATCCAATGCATGCATAAGATCACAGGA
  AGACATGGAAATGAATGTTTTTCCTCAGAAAAACCTGCAGCATACAGGCCATGCCATCTTCAACCCTGCA
  ATGAGAAAATTAATGTAAATACCATAACATCACCCAGACTGGCTGCTCTGACTTTCAAGTGCCTGGGAGA
  TCAGTGGCCAGTGTACTGCCGAGTGATACGTGAAAAGAACCTATGTCAGGACATGCGGTGGTATCAGCGC
  TGCTGTGAAACATGCAGGGACTTCTATGCCCAAAAGCTGCAGCAGAAGAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213075 representing NM_133638
  Red=Cloning site Green=Tags(s)
  
  MRLTHICCCCLLYQLGFLSNGIVSELQFAPDREEWEVVFPALWRREPVDPAGGSGGSADPGWVRGVGGGG
  SARAQAAGSSREVRSVAPVPLEEPVEGRSESRLRPPPPSEGEEDEELESQELPRGSSGAAALSPGAPASW
  QPPPPPQPPPSPPPAQHAEPDGDEVLLRIPAFSRDLYLLLRRDGRFLAPRFAVEQRPNPGPGPTGAASAP
  QPPAPPDAGCFYTGAVLRHPGSLASFSTCGGGLMGFIQLNEDFIFIEPLNDTMAITGHPHRVYRQKRSME
  EKVTEKSALHSHYCGIISDKGRPRSRKIAESGRGKRYSYKLPQEYNIETVVVADPAMVSYHGADAARRFI
  LTILNMVFNLFQHKSLSVQVNLRVIKLILLHETPPELYIGHHGEKMLESFCKWQHEEFGKKNDIHLEMST
  NWGEDMTSVDAAILITRKDFCVHKDEPCDTVGIAYLSGMCSEKRKCIIAEDNGLNLAFTIAHEMGHNMGI
  NHDNDHPSCADGLHIMSGEWIKGQNLGDVSWSRCSKEDLERFLRSKASNCLLQTNPQSVNSVMVPSKLPG
  MTYTADEQCQILFGPLASFCQEMQHVICTGLWCKVEGEKECRTKLDPPMDGTDCDLGKWCKAGECTSRTS
  APEHLAGEWSLWSPCSRTCSAGISSRERKCPGLDSEARDCNGPRKQYRICENPPCPAGLPGFRDWQCQAY
  SVRTSSPKHILQWQAVLDEEKPCALFCSPVGKEQPILLSEKVMDGTSCGYQGLDICANGRCQKVGCDGLL
  GSLAREDHCGVCNGNGKSCKIIKGDFNHTRGAGYVEVLVIPAGARRIKVVEEKPAHSYLALRDAGKQSIN
  SDWKIEHSGAFNLAGTTVHYVRRGLWEKISAKGPTTAPLHLLVLLFQDQNYGLHYEYTIPSDPLPENQSS
  KAPEPLFMWTHTSWEDCDATCGGGERKTTVSCTKIMSKNISIVDNEKCKYLTKPEPQIRKCNEQPCQTRW
  MMTEWTPCSRTCGKGMQSRQVACTQQLSNGTLIRARERDCIGPKPASAQRCEGQDCMTVWEAGVWSECSV
  KCGKGIRHRTVRCTNPRKKCVLSTRPREAEDCEDYSKCYVWRMGDWSKCSITCGKGMQSRVIQCMHKITG
  RHGNECFSSEKPAAYRPCHLQPCNEKINVNTITSPRLAALTFKCLGDQWPVYCRVIREKNLCQDMRWYQR
  CCETCRDFYAQKLQQKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133638
• ORF Size: 3621 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133638.3
• RefSeq Size: 5094 bp
• RefSeq ORF: 3642 bp
• Locus ID: 171019
• UniProt ID: Q8TE59
• Cytogenetics: 5q23.3
• Protein Families: Protease, Secreted Protein
• MW: 131.6 kDa
• Gene Summary: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene has high sequence similarity to the protein encoded by ADAMTS16, another family member. [provided by RefSeq, Jul 2008]