Cytokeratin 2 (KRT2) (NM_000423) Human Tagged ORF Clone

CAT#: RC213030

KRT2 (Myc-DDK-tagged)-Human keratin 2 (KRT2)

Product Images

Circular map for RC213030

• 

  HEK293T cells were transfected with the overexpression plasmids of 14 KRT isoforms (from left to right:KRT1, Cat# RC223146; KRT2, Cat# RC213030; KRT3, Cat# RC223011; KRT4, Cat# RC220350; KRT5, Cat# RC206870; KRT6a, Cat# RC204328; KRT7, Cat# RC201124; KRT8, Cat# RC209570;KRT9, Cat# RC218091; KRT10, Cat# RC204500;KRT12, Cat# RC224946;KRT13, Cat# RC201179;KRT14, Cat# RC214907;KRT15, Cat# RC201150) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-flag antibody (Cat# TA180144, 1:1000) or anti-KRT7 mouse monoclonal antibody (Cat# TA803333, 1:500).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cytokeratin 2
• Synonyms: CK-2e; K2e; KRT2A; KRT2E; KRTE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213030 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTGTCAGATCTCTTGCAAATCTCGAGGAAGAGGAGGAGGTGGAGGAGGATTCCGGGGCTTCAGCA
  GCGGCTCAGCTGTGGTGTCTGGTGGAAGCCGGAGATCAACTTCCAGCTTCTCCTGCTTGAGCCGCCATGG
  TGGTGGTGGCGGGGGCTTCGGTGGAGGCGGCTTTGGCAGTCGGAGTCTTGTTGGCCTTGGAGGGACCAAG
  AGCATCTCCATTAGTGTGGCTGGAGGAGGTGGTGGCTTTGGCGCCGCTGGTGGATTTGGTGGCAGAGGAG
  GTGGTTTTGGAGGCGGCAGCAGCTTTGGAGGTGGCAGCGGCTTCAGTGGTGGTGGTTTCGGTGGAGGCGG
  CTTTGGTGGAGGCCGCTTTGGAGGTTTTGGGGGCCCTGGTGGTGTTGGAGGTTTAGGGGGTCCTGGTGGC
  TTTGGGCCTGGAGGATACCCTGGTGGCATCCACGAAGTCTCTGTCAACCAGAGCCTCCTGCAGCCTCTCA
  ACGTGAAAGTTGACCCAGAGATCCAGAATGTGAAGGCCCAAGAGCGTGAGCAGATCAAAACTCTCAACAA
  CAAATTTGCCTCCTTCATTGACAAGGTGCGGTTCTTGGAGCAGCAGAACCAGGTGTTACAGACCAAATGG
  GAGCTGCTACAACAAATGAATGTTGGCACCCGCCCCATCAACCTGGAGCCCATCTTCCAGGGGTATATCG
  ACAGCCTCAAGAGATATCTGGATGGGCTCACTGCAGAAAGAACATCACAGAATTCAGAGCTGAATAACAT
  GCAGGATCTTGTGGAGGATTATAAGAAGAAGTATGAGGATGAAATCAATAAGCGCACAGCTGCTGAGAAT
  GATTTTGTGACGCTTAAAAAGGACGTGGACAATGCCTACATGATAAAGGTGGAGTTGCAGTCCAAGGTGG
  ACCTGCTGAACCAGGAAATTGAGTTTCTGAAAGTTCTCTATGATGCGGAGATATCCCAGATACATCAGAG
  TGTCACTGACACCAACGTCATCCTCTCCATGGACAACAGCCGCAACCTGGACTTGGATAGCATCATCGCC
  GAGGTCAAGGCCCAGTATGAGGAGATCGCCCAGAGGAGCAAGGAAGAAGCGGAGGCCCTGTACCACAGCA
  AGTATGAGGAGCTCCAGGTGACTGTCGGGAGACATGGAGACAGCCTGAAAGAGATCAAGATAGAGATCAG
  CGAGCTGAACCGCGTGATCCAGAGGCTGCAGGGGGAGATCGCACATGTGAAGAAGCAGTGTAAGAATGTG
  CAAGATGCCATCGCAGATGCCGAGCAGCGTGGGGAGCATGCCCTCAAGGATGCCAGGAACAAGTTGAATG
  ACCTGGAGGAGGCCCTGCAGCAGGCCAAGGAGGACTTGGCGCGGCTGCTGCGTGACTACCAGGAGCTGAT
  GAACGTGAAGCTGGCCCTAGATGTGGAGATCGCCACCTACCGCAAACTGCTGGAGGGCGAGGAGTGCAGG
  ATGTCTGGAGACCTCAGCAGCAATGTGACTGTGTCTGTGACAAGCAGCACCATTTCATCAAATGTGGCAT
  CCAAGGCTGCCTTTGGAGGTTCTGGAGGTAGAGGGTCCAGTTCCGGAGGAGGATACAGCTCTGGAAGCAG
  CAGTTATGGCTCTGGAGGCCGACAGTCTGGCTCCAGAGGCGGTAGTGGAGGAGGAGGTTCTATCTCTGGA
  GGAGGATATGGCTCTGGCGGTGGTTCTGGAGGAAGATACGGATCTGGTGGTGGCTCTAAGGGAGGGTCCA
  TCTCTGGAGGAGGATATGGCTCTGGAGGTGGAAAACACAGCTCTGGAGGTGGCTCTAGAGGAGGCTCCAG
  CTCTGGAGGAGGATATGGCTCTGGAGGTGGGGGTTCTAGCTCTGTAAAGGGTAGCTCAGGTGAAGCTTTT
  GGTTCCAGCGTGACCTTCTCTTTTAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213030 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSCQISCKSRGRGGGGGGFRGFSSGSAVVSGGSRRSTSSFSCLSRHGGGGGGFGGGGFGSRSLVGLGGTK
  SISISVAGGGGGFGAAGGFGGRGGGFGGGSSFGGGSGFSGGGFGGGGFGGGRFGGFGGPGGVGGLGGPGG
  FGPGGYPGGIHEVSVNQSLLQPLNVKVDPEIQNVKAQEREQIKTLNNKFASFIDKVRFLEQQNQVLQTKW
  ELLQQMNVGTRPINLEPIFQGYIDSLKRYLDGLTAERTSQNSELNNMQDLVEDYKKKYEDEINKRTAAEN
  DFVTLKKDVDNAYMIKVELQSKVDLLNQEIEFLKVLYDAEISQIHQSVTDTNVILSMDNSRNLDLDSIIA
  EVKAQYEEIAQRSKEEAEALYHSKYEELQVTVGRHGDSLKEIKIEISELNRVIQRLQGEIAHVKKQCKNV
  QDAIADAEQRGEHALKDARNKLNDLEEALQQAKEDLARLLRDYQELMNVKLALDVEIATYRKLLEGEECR
  MSGDLSSNVTVSVTSSTISSNVASKAAFGGSGGRGSSSGGGYSSGSSSYGSGGRQSGSRGGSGGGGSISG
  GGYGSGGGSGGRYGSGGGSKGGSISGGGYGSGGGKHSSGGGSRGGSSSGGGYGSGGGGSSSVKGSSGEAF
  GSSVTFSFR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000423
• ORF Size: 1917 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000423.3
• RefSeq Size: 2459 bp
• RefSeq ORF: 1920 bp
• Locus ID: 3849
• UniProt ID: P35908
• Cytogenetics: 12q13.13
• MW: 65.4 kDa
• Gene Summary: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]