RIM1 (RIMS1) (NM_014989) Human Tagged ORF Clone

CAT#: RC213013

RIMS1 (Myc-DDK-tagged)-Human regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant 1

Product Images

Circular map for RC213013

• 

  Western blot validation of overexpression lysate (Cat# LY414883) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213013 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RIM1
• Synonyms: CORD7; RAB3IP2; RIM; RIM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213013 representing NM_014989
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGTCCTCGGCCGTGGGGCCCCGCGGTCCTCGCCCACCCACGGTGCCTCCCCCCATGCAAGAGCTGCCCG
  ACCTGAGCCACCTGACCGAAGAGGAGAGGAACATTATCATGGCAGTGATGGACCGGCAGAAGGAAGAGGA
  GGAAAAAGAAGAAGCCATGCTCAAGTGTGTTGTCAGGGACATGGCGAAGCCTGCTGCCTGCAAAACACCA
  AGAAATGCTGAAAACCAGCCCCACCAACCTTCACCGAGATTGCATCAACAGTTTGAAAGCTATAAGGAAC
  AAGTGAGAAAAATAGGGGAAGAAGCGCGGCGTTACCAGGGCGAGCACAAAGACGATGCTCCGACTTGTGG
  AATCTGTCATAAAACAAAGTTTGCTGATGGGTGCGGTCATCTCTGCTCCTATTGTCGCACTAAGTTCTGT
  GCGCGCTGCGGAGGCCGCGTGTCTCTACGGTCAAACAACGAGGACAAAGTGGTTATGTGGGTATGCAATT
  TATGTCGAAAGCAACAAGAAATCTTAACCAAATCTGGGGCATGGTTCTTTGGAAGTGGCCCTCAGCAGAC
  AAGTCAGGATGGAACCCTGAGTGATACAGCTACAGGTGCTGGCTCTGAGGTACCAAGAGAAAAGAAAGCA
  CGACTCCAAGAGCGATCGCGGTCTCAGACACCCCTAAGCACAGCAGCTGCCTCCTCCCAGGATGCTGCTC
  CTCCCAGCGCACCACCAGACAGGAGCAAAGGGGCTGAGCCCTCGCAGCAAGCCTTGGGGCCTGAACAGAA
  GCAGGCTTCATCCAGGTCTAGAAGTGAACCTCCTAGAGAGAGAAAGAAGACCCCAGGGCTTTCCGAGCAG
  AATGGCAAAGGAGCCCTGAAGAGCGAGCGGAAACGCGTGCCAAAGACCTCAGCGCAGCCCGTGGAGGGGG
  CCGTCGAAGAACGGGAGCGCAAAGAAAGGCGGGAAAGCCGAAGGCTTGAGAAAGGGCGATCACAGGATTA
  CCCAGACACGCCGGAAAAACGGGATGAGGGCAAAGCGGCGGATGAGGAAAAGCAAAGAAAAGAGGAGGAT
  TATCAGACCAGGTACCGCAGCGACCCGAACCTAGCTCGGTACCCGGTGAAACCGCCGCCTGAGGAGCAGC
  AGATGCGCATGCACGCCCGGGTGTCCCGCGCCAGGCACGAGCGGCGCCACAGCGACGTGGCGCTCCCGCG
  CACCGAGGCGGGCGCGGCGCTGCCGGAGGGCAAGGCCGGCAAACGCGCGCCGGCGGCAGCCAGGGCCTCG
  CCGCCGGACTCGCCGCGGGCTTACTCGGCTGAGAGAACTGCGGAGACCAGGGCGCCGGGCGCCAAGCAGC
  TAACGAACCACAGCCCGCCGGCGCCCAGACATGGGCCGGTTCCCGCAGAAGCCCCGGAGCTCAAAGCCCA
  GGAGCCCCTCAGGAAGCAGAGCCGCCTGGACCCCAGCTCGGCGGTCCTCATGCGGAAGGCCAAGCGCGAG
  AAGGTGGAGACCATGCTGCGGAACGACTCTTTGAGCTCAGACCAGTCCGAGTCGGTGCGGCCGTCCCCGC
  CCAAGCCGCACCGGTCCAAGAGAGGCGGCAAGAAGCGGCAGATGTCGGTGAGCAGCTCTGAGGAGGAGGG
  CGTGTCGACGCCCGAGTACACCAGCTGCGAGGACGTGGAGCTGGAGAGCGAGAGCGTCAGCGAGAAAGGT
  GATTTGGATTATTACTGGTTGGATCCTGCCACGTGGCACAGCCGGGAGACATCACCTATTAGTTCGCATC
  CTGTAACGTGGCAACCATCTAAAGAGGGGGACCGATTAATTGGACGTGTTATTCTTAACAAGAGAACAAC
  CATGCCCAAAGACTCAGGTGCATTGCTGGGTCTGAAAGTTGTTGGAGGAAAAATGACTGACTTAGGACGA
  CTTGGTGCTTTCATCACCAAAGTAAAGAAGGGTAGCCTAGCAGATGTAGTTGGACACCTAAGAGCAGGGG
  ATGAAGTTCTAGAATGGAATGGTAAACCCCTGCCGGGAGCTACAAATGAAGAAGTTTACAACATTATTTT
  AGAATCAAAATCAGAACCTCAAGTTGAAATTATTGTTTCAAGGCCTATTGGTGACATTCCCCGGATTCCT
  GAGAGCTCCCACCCTCCACTGGAGTCCAGTTCAAGTTCCTTTGAATCTCAGAAGATGGAAAGGCCTTCCA
  TTTCTGTTATTTCTCCAACAAGTCCTGGAGCTCTAAAAGATGCCCCACAAGTCTTACCAGGGCAACTTTC
  TGTGAAGTTGTGGTATGATAAAGTGGGACACCAGCTGATTGTAAATGTTCTGCAAGCAACAGATCTACCT
  GCTAGAGTAGATGGACGTCCTCGAAATCCCTATGTAAAAATGTATTTTCTTCCAGATAGAAGTGATAAAA
  GTAAAAGGAGGACCAAAACAGTAAAGAAAATACTAGAACCAAAATGGAATCAAACTTTTGTCTATTCACA
  TGTACATCGTAGAGATTTTAGAGAACGAATGTTAGAAATAACTGTGTGGGACCAACCAAGAGTGCAAGAA
  GAAGAAAGTGAATTTCTTGGAGAGATCCTCATAGAATTGGAGACAGCGCTTTTAGATGATGAACCGCATT
  GGTATAAACTTCAGACACATGATGAGTCTTCACTACCTCTGCCTCAGCCATCACCTTTCATGCCAAGGCG
  ACATATTCATGGAGAAAGCTCTAGCAAAAAGCTACAAAGATCTCAGCGAATCAGTGATAGTGACATCTCA
  GATTATGAGGTTGATGATGGTATTGGCGTAGTTCCTCCAGTAGGCTATAGGTCTAGTGCTAGAGAAAGTA
  AATCTACAACATTAACTGTGCCAGAACAGCAAAGAACAACTCATCACCGCTCACGTTCAGTATCTCCTCA
  TCGCGGCAATGATCAGGGAAAGCCGCGTTCACGTTTACCAAATGTGCCATTACAGAGGAGTTTAGATGAA
  ATTCATCCAACAAGAAGGTCACGTTCTCCAACCAGACACCATGATGCCTCCCGAAGTCCAGTTGATCATA
  GAACCAGAGATGTGGATAGTCAGTATTTATCAGAACAAGACAGTGAGCTTCTTATGCTGCCCAGAGCAAA
  ACGAGGACGAAGTGCAGAATGCCTACATACTACCAGACATCTTGTTAGGCACTATAAAACATTACCTCCC
  AAGATGCCTTTATTACAGAGCAGTTCTCACTGGAATATTTACAGCTCAATTCTGCCTGCACATACTAAGA
  CCAAATCAGTGACTAGACAGGACATTTCCCTTCATCATGAATGCTTTAACTCAACAGTATTGAGATTTAC
  TGATGAAATACTGGTTAGTGAACTGCAGCCCTTTCTTGACAGGGCTAGGAGTGCTAGTACCAACTGCTTG
  AGACCAGATACTAGTTTGCATTCACCAGAACGAGAAAGGGGTAGATGGTCCCCCTCCCTAGATAGGAGAC
  GACCTCCTAGTCCCAGGATTCAAATCCAGCATGCGTCTCCGGAGAATGACAGGCACTCCAGAAAGTCTGA
  AAGATCTAGCATCCAAAAACAGACTAGGAAAGGCACTGCCTCTGATGCAGAAAGGGTTCTCCCAACATGT
  CTTTCTAGAAGGGGACACGCAGCCCCAAGAGCAACTGATCAGCCAGTCATTAGGGGAAAACATCCTGCTC
  GCTCAAGGTCGAGTGAGCACTCTAGTATCAGAACACTGTGTTCTATGCACCACCTTGTCCCTGGAGGGTC
  GGCGCCACCTTCTCCGCTTCTGACAAGAATGCACCGACAGAGAAGTCCAACACAATCTCCTCCAGCAGAC
  ACATCGTTCAGCAGTCGCAGGGGAAGACAGCTCCCACAAGTGCCAGTGAGAAGCGGCAGTATAGAACAAG
  CAAGCTTAGTAGTGGAGGAGCGAACAAGACAGATGAAAATGAAAGTGCATCGATTTAAGCAGACAACAGG
  GTCTGGTTCTAGTCAAGAACTTGATCGCGAGCAATATTCCAAGTATAACATACATAAAGATCAGTACAGA
  AGCTGTGATAACGTCTCTGCCAAATCATCAGATAGTGATGTCAGTGATGTTTCCGCCATTTCCCGAACCA
  GCAGTGCCTCACGCCTCAGCAGCACAAGCTTTATGTCAGAGCAATCTGAGCGCCCCAGGGGTAGAATCAG
  TTCATTTACCCCCAAAATGCAAGGCAGACGGATGGGGACTTCAGGAAGATCCATCATGAAGAGCACCAGT
  GTCAGTGGAGAGATGTACACACTGGAGCATAATGACGGCAGCCAGTCAGACACAGCTGTGGGTACAGTTG
  GAGCAGGTGGAAAGAAACGGAGATCCAGCCTTAGTGCCAAAGTGGTTGCCATAGTGTCTCGAAGGAGTAG
  AAGCACATCCCAGCTTAGTCAAACAGAGTCGGGCCACAAAAAGTTAAAAAGTACCATCCAGAGAAGCACA
  GAAACAGGCATGGCAGCTGAAATGAGAAAGATGGTAAGGCAGCCGAGCCGAGAGTCTACTGATGGCAGCA
  TCAACAGTTACAGCTCTGAGGGCAATTTAATATTTCCTGGAGTGCGACTGGGAGCTGACAGTCAATTCAG
  TGATTTTCTTGATGGATTGGGACCAGCCCAGCTTGTTGGCCGCCAAACCCTTGCCACCCCTGCAATGGGT
  GATATACAAATAGGAATGGAGGACAAAAAGGGCCAATTAGAAGTGGAAGTCATTAGAGCACGAAGCCTCA
  CACAAAAGCCTGGTTCCAAATCTACACCTGCTCCATATGTCAAAGTATATCTTTTGGAAAATGGGGCCTG
  TATAGCCAAGAAGAAGACAAGAATTGCACGAAAAACCCTTGATCCTTTGTATCAGCAGTCTCTGGTTTTT
  GATGAAAGTCCACAGGGTAAAGTTCTTCAGGTGATTGTCTGGGGAGACTATGGCAGAATGGACCACAAAT
  GCTTTATGGGTGTGGCTCAGATCTTGTTGGAAGAACTCGACCTGTCCAGCATGGTGATCGGATGGTACAA
  ATTGTTCCCACCGTCCTCACTGGTGGATCCCACACTCACTCCCCTCACCCGGCGGGCTTCCCAGTCATCT
  CTGGAAAGTTCAACTGGGCCTCCCTGTATTCGATCA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213013 representing NM_014989
  Red=Cloning site Green=Tags(s)
  
  MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTP
  RNAENQPHQPSPRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFC
  ARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQTSQDGTLSDTATGAGSEVPREKKA
  RLQERSRSQTPLSTAAASSQDAAPPSAPPDRSKGAEPSQQALGPEQKQASSRSRSEPPRERKKTPGLSEQ
  NGKGALKSERKRVPKTSAQPVEGAVEERERKERRESRRLEKGRSQDYPDTPEKRDEGKAADEEKQRKEED
  YQTRYRSDPNLARYPVKPPPEEQQMRMHARVSRARHERRHSDVALPRTEAGAALPEGKAGKRAPAAARAS
  PPDSPRAYSAERTAETRAPGAKQLTNHSPPAPRHGPVPAEAPELKAQEPLRKQSRLDPSSAVLMRKAKRE
  KVETMLRNDSLSSDQSESVRPSPPKPHRSKRGGKKRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKG
  DLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKDSGALLGLKVVGGKMTDLGR
  LGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIP
  ESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLP
  ARVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKILEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQE
  EESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDIS
  DYEVDDGIGVVPPVGYRSSARESKSTTLTVPEQQRTTHHRSRSVSPHRGNDQGKPRSRLPNVPLQRSLDE
  IHPTRRSRSPTRHHDASRSPVDHRTRDVDSQYLSEQDSELLMLPRAKRGRSAECLHTTRHLVRHYKTLPP
  KMPLLQSSSHWNIYSSILPAHTKTKSVTRQDISLHHECFNSTVLRFTDEILVSELQPFLDRARSASTNCL
  RPDTSLHSPERERGRWSPSLDRRRPPSPRIQIQHASPENDRHSRKSERSSIQKQTRKGTASDAERVLPTC
  LSRRGHAAPRATDQPVIRGKHPARSRSSEHSSIRTLCSMHHLVPGGSAPPSPLLTRMHRQRSPTQSPPAD
  TSFSSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDREQYSKYNIHKDQYR
  SCDNVSAKSSDSDVSDVSAISRTSSASRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRSIMKSTS
  VSGEMYTLEHNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRST
  ETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRLGADSQFSDFLDGLGPAQLVGRQTLATPAMG
  DIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVF
  DESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLTPLTRRASQSS
  LESSTGPPCIRS
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014989
• ORF Size: 5076 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014989.5, NP_055804.2
• RefSeq Size: 5079 bp
• RefSeq ORF: 5079 bp
• Locus ID: 22999
• UniProt ID: Q86UR5
• Cytogenetics: 6q13
• Domains: C2, PDZ, RPH3A_effector
• MW: 188.9 kDa
• Gene Summary: The protein encoded by this gene is a RAS gene superfamily member that regulates synaptic vesicle exocytosis. This gene also plays a role in the regulation of voltage-gated calcium channels during neurotransmitter and insulin release. Mutations have suggested a role cognition and have been identified as the cause of cone-rod dystrophy type 7. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Mar 2012]