RANBP9 (NM_005493) Human Tagged ORF Clone

CAT#: RC212902

RANBP9 (Myc-DDK-tagged)-Human RAN binding protein 9 (RANBP9)

Product Images

Circular map for RC212902

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  Western blot validation of overexpression lysate (Cat# LY401682) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212902 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RANBP9 protein (Cat# TP312902). The protein was produced from HEK293T cells transfected with RANBP9 cDNA clone (Cat# RC212902) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RANBP9
• Synonyms: BPM-L; BPM90; RanBP7; RANBPM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212902 representing NM_005493
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGGCAGCCGCCGCCGCCGCCGCCGCAGCAGCAGCAACAGCAGCAGCAGCTGTCGCCGCCACCGC
  CGGCGGCCTTGGCCCCAGTCTCCGGAGTCGTCCTGCCGGCGCCCCCGGCCGTCAGCGCCGGCTCTTCTCC
  GGCCGGCTCGCCCGGCGGCGGTGCGGGCGGCGAAGGCTTAGGGGCCGCGGCGGCCGCCCTGCTCCTCCAC
  CCTCCGCCGCCGCCGCCCCCGGCCACCGCGGCCCCGCCGCCCCCGCCGCCGCCCCCGCCTCCCCCTGCCT
  CAGCGGCTGCCCCCGCCAGCGGGCCGCCCGCTCCCCCGGGCCTTGCAGCGGGCCCCGGCCCGGCTGGAGG
  AGCCCCGACCCCAGCTCTGGTGGCGGGCAGCAGCGCCGCGGCCCCCTTCCCTCACGGGGACTCGGCCCTG
  AACGAGCAGGAGAAGGAGTTGCAGCGGCGGCTGAAGCGTCTCTACCCGGCCGTGGACGAACAAGAGACGC
  CGCTGCCTCGGTCCTGGAGCCCGAAGGACAAGTTCAGCTACATCGGCCTCTCTCAGAACAACCTGCGGGT
  GCACTACAAAGGTCATGGCAAAACCCCAAAAGATGCCGCGTCAGTTCGAGCCACGCATCCAATACCAGCA
  GCCTGTGGGATTTATTATTTTGAAGTAAAAATTGTCAGTAAGGGAAGAGATGGTTACATGGGAATTGGTC
  TTTCTGCTCAAGGTGTGAACATGAATAGACTACCAGGTTGGGATAAGCATTCATATGGTTACCATGGGGA
  TGATGGACATTCGTTTTGTTCTTCTGGAACTGGACAACCTTATGGACCAACTTTCACTACTGGTGATGTC
  ATTGGCTGTTGTGTTAATCTTATCAACAATACCTGCTTTTACACCAAGAATGGACATAGTTTAGGTATTG
  CTTTCACTGACCTACCGCCAAATTTGTATCCTACTGTGGGGCTTCAAACACCAGGAGAAGTGGTCGATGC
  CAATTTTGGGCAACATCCTTTCGTGTTTGATATAGAAGACTATATGCGGGAGTGGAGAACCAAAATCCAG
  GCACAGATAGATCGATTTCCTATCGGAGATCGAGAAGGAGAATGGCAGACCATGATACAAAAAATGGTTT
  CATCTTATTTAGTCCACCATGGGTACTGTGCCACAGCAGAGGCCTTTGCCAGATCTACAGACCAGACCGT
  TCTAGAAGAATTAGCTTCCATTAAGAATAGACAAAGAATTCAGAAATTGGTATTAGCAGGAAGAATGGGA
  GAAGCCATTGAAACAACACAACAGTTATACCCAAGTTTACTTGAAAGAAATCCTAATCTCCTTTTCACAT
  TAAAAGTGCGTCAGTTTATAGAAATGGTGAATGGTACAGATAGTGAAGTACGATGTTTGGGAGGCCGAAG
  TCCAAAGTCTCAAGACAGTTATCCTGTTAGTCCTCGACCTTTTAGTAGTCCAAGTATGAGCCCCAGCCAT
  GGAATGAATATCCACAATTTAGCATCAGGCAAAGGAAGCACCGCACATTTTTCAGGTTTTGAAAGTTGTA
  GTAATGGTGTAATATCAAATAAAGCACATCAATCATATTGCCATAGTAATAAACACCAGTCATCCAACTT
  GAATGTACCAGAACTAAACAGTATAAATATGTCAAGATCACAGCAAGTTAATAACTTCACCAGTAATGAT
  GTAGACATGGAAACAGATCACTACTCCAATGGAGTTGGAGAAACTTCATCCAATGGTTTCCTAAATGGTA
  GCTCTAAACATGACCACGAAATGGAAGATTGTGACACCGAAATGGAAGTTGATTCAAGTCAGTTGAGACG
  CCAGTTGTGTGGAGGAAGTCAGGCCGCCATAGAAAGAATGATCCACTTTGGACGAGAGCTGCAAGCAATG
  AGTGAACAGCTAAGGAGAGACTGTGGCAAGAACACTGCAAACAAAAAAATGTTGAAGGATGCATTCAGTC
  TACTAGCATATTCAGATCCCTGGAACAGCCCAGTTGGAAATCAGCTTGACCCGATTCAGAGAGAACCTGT
  GTGCTCAGCTCTTAACAGTGCAATATTAGAAACCCACAATCTGCCAAAGCAACCTCCACTTGCCCTAGCA
  ATGGGACAGGCCACACAATGTCTAGGACTGATGGCTCGATCAGGAATTGGATCCTGCGCATTTGCCACAG
  TGGAAGACTACCTACAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212902 representing NM_005493
  Red=Cloning site Green=Tags(s)
  
  MSGQPPPPPPQQQQQQQQLSPPPPAALAPVSGVVLPAPPAVSAGSSPAGSPGGGAGGEGLGAAAAALLLH
  PPPPPPPATAAPPPPPPPPPPPASAAAPASGPPAPPGLAAGPGPAGGAPTPALVAGSSAAAPFPHGDSAL
  NEQEKELQRRLKRLYPAVDEQETPLPRSWSPKDKFSYIGLSQNNLRVHYKGHGKTPKDAASVRATHPIPA
  ACGIYYFEVKIVSKGRDGYMGIGLSAQGVNMNRLPGWDKHSYGYHGDDGHSFCSSGTGQPYGPTFTTGDV
  IGCCVNLINNTCFYTKNGHSLGIAFTDLPPNLYPTVGLQTPGEVVDANFGQHPFVFDIEDYMREWRTKIQ
  AQIDRFPIGDREGEWQTMIQKMVSSYLVHHGYCATAEAFARSTDQTVLEELASIKNRQRIQKLVLAGRMG
  EAIETTQQLYPSLLERNPNLLFTLKVRQFIEMVNGTDSEVRCLGGRSPKSQDSYPVSPRPFSSPSMSPSH
  GMNIHNLASGKGSTAHFSGFESCSNGVISNKAHQSYCHSNKHQSSNLNVPELNSINMSRSQQVNNFTSND
  VDMETDHYSNGVGETSSNGFLNGSSKHDHEMEDCDTEMEVDSSQLRRQLCGGSQAAIERMIHFGRELQAM
  SEQLRRDCGKNTANKKMLKDAFSLLAYSDPWNSPVGNQLDPIQREPVCSALNSAILETHNLPKQPPLALA
  MGQATQCLGLMARSGIGSCAFATVEDYLH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005493
• ORF Size: 2187 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005493.3
• RefSeq Size: 3132 bp
• RefSeq ORF: 2190 bp
• Locus ID: 10048
• UniProt ID: Q96S59
• Cytogenetics: 6p23
• Domains: SPRY, LisH, CTLH
• Protein Families: Druggable Genome
• MW: 77.7 kDa
• Gene Summary: This gene encodes a protein that binds RAN, a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The protein encoded by this gene has also been shown to interact with several other proteins, including met proto-oncogene, homeodomain interacting protein kinase 2, androgen receptor, and cyclin-dependent kinase 11. [provided by RefSeq, Jul 2008]