SYT16 (NM_031914) Human Tagged ORF Clone

CAT#: RC212871

SYT16 (Myc-DDK-tagged)-Human synaptotagmin XVI (SYT16)

Product Images

Circular map for RC212871

• 

  Western blot validation of overexpression lysate (Cat# LY410443) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212871 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SYT16
• Synonyms: CHR14SYT; Strep14; SYT14L; syt14r; yt14r
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212871 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTTGGCCATGGCGTCTCAGGATGCTCAGAACTTCTTCCAGCCTTTCTCTTCCTGGATATCTCGGG
  TTTATGAAGCTCTCCAGCAAGCAGGAGATATGTTATCTGCTTCGCTGGTTAACATAAGCAAACAAGACTC
  TAAATTGAGTGACAAACTAGATCAGGACTTAGATAATATTCAGATTCAGGAAACGTACTTTGAAGATGAA
  GAACAAGACAATGATTGGAGTCAAGAGGATGCAAATTCCTTGTTTCTTGAAGTGGATCATTTCTCATGTT
  GTAATAGTGATTTGCAGGACTCTGCCCAAAATTCAAGCCCAAGCCTTAGCCAACATGCAAAGGACTCATG
  TTCCACAATGTCCCAGTGGCCCAATTGGGCCAGTGATGACCGCAAGTTACCACATGTGCTTTCTTCTATT
  GCGGAGGAAGAGCATCACCTTGAAAAGCAAAGAAGTGGCCTTCAACATGGCTTTGACAGCCAGCTCCCTG
  GTACTTTAGAAACTGTTAATGGAAAAAAGCAAGTCAACAGCTTTGGGGATGACGAAGAGCTGTCCACATC
  TTCTGACAGTGACGAGGAGGTGATCAAACAATTTGAGATTTCCGTGTCCCGGTCCCAGAGTTTCCGTTCA
  GTGACATCTGAGAAAGGAAAGCAGACAGGATTGGAGCAGAAACCAAAATTCAGCCGTTCGTTGTTGACAC
  ACGGAGAAGATGGCACAGAAGTATCTGCCTGCGAAGATTTGGATGGAGCCAGCCAACGGCGTTATTCTGA
  GAATCTCTCCTACGGTGAAGATGACCACATCCCTGCTCACTCACAGTCCCCATGTGAAAGAGGGGATGCC
  AAACACCACGGCACATCTCACCAAGAGTCCAGTGTGGTCCAAAGCCTCAGGCGCCAATCCACAGAGGGCA
  GCTTGGAGATGGAGACAGCTTTTAATAGCCGGGGATTTGAAGATTCCTATGCCACTGACAGCTCCTCCAT
  GTGGAGTCCAGAGGAACAGGACAGGACCAATTTGCAGGTGCCATCCGGGGTCTCAGAGCCCATCTCAAAG
  TGTGGTGACCTAGATGTCATCTTTGAATATAGAGCCGCCAGCCAGAAGCTCACAGTGACCATTGTGAGGG
  CACAGGGCCTCCCAGATAAGGACCGAAGTGGTGTCAACTCCTGGCAAGTTCATGTAGTGCTGCTGCCTGG
  TAAGAAACACAGGGGCAGGACGAACATACAGAGAGGGCCCAACCCCGTCTTCAGGGAGAAGGTCACCTTT
  GCCAAGCTGGAGCCCAGAGATGTGGCTGCCTGTGCTGTCCGCTTCCGCCTGTACGCTGCCCGGAAGATGA
  CCCGAGAGAGAATGATGGGAGAGAAACTATTCTATCTCAGCCACCTGCACCCAGAAGGGGAAATGAAAGT
  GACTCTGGTTCTGGAGCCAAGAAGTAATATAAGCAGTGGAGGGTCTCCGCTCAGCCCATCTGCGGTTTCT
  CACAGTGATAGTACTTCATCCACGCAGTCGCTGTCTCATGGAGGGGCGCCAGAGCTGTTGGTGGGGCTCT
  CGTACAATGCCACAACGGGGCGATTATCTGTGGAAATGATCAAAGGCAGCCATTTCCGAAACCTCGCTGT
  TAACCGAGCACCTGATACATATGGAAAACTCTTTCTCCTCAATTCTGTGGGTCAAGAGATGTCCCGTTGC
  AAGACGTCCATTCGGCGTGGTCAGCCCAATCCTGTCTATAAGGAGACCTTTGTTTTCCAGGTGGCCCTCT
  TTCAGCTGTCTGATGTCACGTTGATGATTTCCGTTTATAACAGGCGTACTATGAAGCGTAAAGAGATGAT
  TGGCTGGATTGCCCTGGGCCAGAACAGCAGTGGAGAGGAGGAACAAGATCACTGGGAGGAGATGAAGGAA
  ACCAAAGGCCAGCAGATCTGCAGATGGCACACTTTGCTGGAATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212871 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVLAMASQDAQNFFQPFSSWISRVYEALQQAGDMLSASLVNISKQDSKLSDKLDQDLDNIQIQETYFEDE
  EQDNDWSQEDANSLFLEVDHFSCCNSDLQDSAQNSSPSLSQHAKDSCSTMSQWPNWASDDRKLPHVLSSI
  AEEEHHLEKQRSGLQHGFDSQLPGTLETVNGKKQVNSFGDDEELSTSSDSDEEVIKQFEISVSRSQSFRS
  VTSEKGKQTGLEQKPKFSRSLLTHGEDGTEVSACEDLDGASQRRYSENLSYGEDDHIPAHSQSPCERGDA
  KHHGTSHQESSVVQSLRRQSTEGSLEMETAFNSRGFEDSYATDSSSMWSPEEQDRTNLQVPSGVSEPISK
  CGDLDVIFEYRAASQKLTVTIVRAQGLPDKDRSGVNSWQVHVVLLPGKKHRGRTNIQRGPNPVFREKVTF
  AKLEPRDVAACAVRFRLYAARKMTRERMMGEKLFYLSHLHPEGEMKVTLVLEPRSNISSGGSPLSPSAVS
  HSDSTSSTQSLSHGGAPELLVGLSYNATTGRLSVEMIKGSHFRNLAVNRAPDTYGKLFLLNSVGQEMSRC
  KTSIRRGQPNPVYKETFVFQVALFQLSDVTLMISVYNRRTMKRKEMIGWIALGQNSSGEEEQDHWEEMKE
  TKGQQICRWHTLLES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031914
• ORF Size: 1935 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031914.2, NP_114120.2
• RefSeq Size: 3137 bp
• RefSeq ORF: 1938 bp
• Locus ID: 83851
• UniProt ID: Q17RD7
• Cytogenetics: 14q23.2
• MW: 72.2 kDa
• Gene Summary: May be involved in the trafficking and exocytosis of secretory vesicles in non-neuronal tissues. Is Ca(2+)-independent.[UniProtKB/Swiss-Prot Function]