QRICH2 (NM_032134) Human Tagged ORF Clone

CAT#: RC212861

QRICH2 (Myc-DDK-tagged)-Human glutamine rich 2 (QRICH2). Note: ORF is codon optimized

Product Images

Circular map for RC212861

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: QRICH2
• Synonyms: SPGF35
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212861 ORF sequence, codon optimized.
  Due to the complexity of NM_032134, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGACGCCGCCGAAGAGCTGTCATTCGCACGAGTACTGCTGCAGAGGGTGGACGAGCTGGAGAAAC
  TGTTCAAAGATAGAGAACAGTTCCTTGAATTGGTCAGCCGCAAGCTGAGCCTGGTCCCCGGTGCCGAGGA
  AGTTACCATGGTCACTTGGGAGGAACTCGAACAGGCAATCACCGACGGCTGGCGAGCCAGCCAGGCGGGC
  TCAGAAACTCTTATGGGTTTCAGCAAGCATGGAGGCTTTACAAGCCTTACCTCTCCCGAGGGAACTCTGT
  CCGGAGACTCAACAAAGCAGCCAAGTATTGAGCAAGCCCTCGACTCCGCTAGCGGGCTGGGGCCAGATCG
  CACTGCGAGTGGAAGCGGAGGGACTGCACACCCTTCTGACGGGGTCAGCTCCAGAGAGCAAAGTAAGGTC
  CCGAGCGGAACAGGTCGACAGCAGCAGCCTCGAGCTAGGGACGAGGCGGGGGTCCCCAGGCTGCATCAAA
  GTAGTACATTCCAATTCAAGTCAGATTCCGACCGCCACAGGTCAAGGGAAAAGCTCACTAGCACACAACC
  ACGCAGAAACGCCAGACCGGGGCCAGTTCAGCAAGACCTGCCTCTCGCAAGGGACCAGCCCTCTTCAGTC
  CCCGCCAGCCAGTCCCAGGTGCACCTCAGGCCCGATAGGCGCGGGCTCGAGCCCACAGGTATGAATCAAC
  CCGGGTTGGTACCCGCTTCAACCTATCCGCACGGTGTCGTGCCACTGTCTATGGGGCAACTGGGCGTACC
  GCCGCCCGAGATGGACGACCGGGAACTTATTCCCTTCGTGGTCGACGAACAGCGGATGCTCCCCCCCAGC
  GTCCCTGGTAGGGACCAACAGGGCCTGGAACTGCCCAGTACGGACCAGCACGGCCTCGTGAGTGTATCCG
  CATATCAACATGGAATGACATTCCCCGGTACCGACCAACGGTCTATGGAACCCCTCGGGATGGACCAGCG
  GGGGTGTGTCATTTCAGGTATGGGGCAGCAGGGGCTTGTGCCACCCGGTATCGACCAGCAGGGACTTACT
  TTGCCCGTCGTCGATCAGCATGGTCTGGTACTGCCCTTCACAGATCAGCATGGGCTGGTGAGTCCTGGGT
  TGATGCCAATCAGCGCTGACCAACAGGGCTTCGTGCAGCCTAGTCTTGAGGCAACAGGGTTCATTCAGCC
  TGGTACCGAACAACATGACCTGATCCAGTCTGGGAGATTTCAAAGAGCCCTGGTTCAGAGAGGAGCTTAC
  CAGCCTGGACTCGTTCAACCAGGCGCCGACCAGCGCGGGTTGGTGAGACCAGGAATGGACCAATCCGGCC
  TCGCCCAGCCGGGTGCCGACCAACGAGGCCTCGTCTGGCCCGGAATGGACCAGTCCGGGCTGGCCCAGCC
  TGGGAGGGATCAACATGGCCTCATACAACCTGGTACCGGGCAACACGACTTGGTGCAGAGCGGCACGGGC
  CAGGGCGTGCTTGTCCAACCTGGGGTGGACCAGCCTGGCATGGTCCAGCCCGGCAGATTCCAGCGCGCTC
  TGGTGCAGCCAGGCGCTTATCAACCTGGGCTGGTGCAGCCAGGGGCCGACCAGATAGATGTGGTCCAGCC
  TGGAGCTGACCAGCACGGACTGGTCCAGTCAGGGGCAGACCAGTCTGACCTCGCACAGCCTGGCGCAGTA
  CAACACGGCCTGGTTCAACCCGGCGTCGACCAGCGAGGATTGGCTCAACCCCGGGCCGACCATCAGAGGG
  GACTGGTTCCCCCCGGTGCCGACCAAAGAGGACTGGTGCAGCCTGGCGCAGACCAGCATGGGTTGGTCCA
  GCCTGGGGTAGACCAGCACGGGCTGGCCCAGCCTGGGGAAGTGCAGAGGAGCTTGGTTCAGCCGGGAATC
  GTACAGAGGGGCCTCGTCCAGCCAGGAGCCGTGCAGCGAGGCCTCGTGCAGCCAGGCGCCGTGCAACGCG
  GGCTGGTGCAGCCTGGAGTAGACCAGAGAGGACTCGTCCAGCCCGGTGCCGTTCAGCGGGGTCTCGTTCA
  GCCGGGCGCAGTGCAACATGGACTGGTGCAGCCCGGTGCTGACCAGAGGGGCCTTGTTCAACCGGGCGTG
  GACCAGAGAGGGTTGGTGCAACCAGGCGTAGACCAAAGAGGCCTGGTGCAGCCAGGCATGGACCAGCGAG
  GCCTGATACAACCCGGCGCAGACCAGCCCGGCCTGGTACAGCCTGGGGCCGGGCAGCTGGGAATGGTCCA
  GCCCGGCATCGGGCAGCAGGGCATGGTGCAACCCCAGGCTGACCCGCATGGTCTCGTGCAGCCCGGCGCA
  TATCCCCTCGGACTGGTTCAGCCAGGAGCGTACCTGCACGATCTGAGTCAGTCAGGGACATACCCTCGGG
  GCCTGGTGCAGCCAGGCATGGATCAGTACGGGCTTAGACAGCCTGGAGCCTATCAGCCTGGCTTGATCGC
  GCCCGGGACTAAGCTGCGGGGGTCAAGCACCTTTCAAGCCGATAGCACGGGATTTATCTCAGTGCGGCCC
  TACCAGCACGGTATGGTTCCACCCGGGCGGGAGCAGTACGGCCAGGTGAGTCCGCTGCTCGCCTCTCAAG
  GTCTGGCCTCCCCTGGAATAGACCGCCGATCACTGGTCCCTCCTGAAACCTACCAGCAGGGACTCATGCA
  TCCCGGGACAGACCAACATTCTCCAATACCCCTGTCTACTGGATTGGGCAGTACCCATCCTGACCAGCAG
  CACGTGGCTTCCCCAGGACCTGGGGAGCACGACCAGGTATATCCTGATGCGGCGCAACATGGCCATGCAT
  TCTCCCTGTTCGACAGTCATGACTCAATGTATCCTGGTTATAGAGGTCCAGGGTACTTGTCAGCAGACCA
  GCACGGCCAGGAGGGCCTGGATCCTAACCGGACGAGAGCTAGTGATCGACACGGCATACCCGCCCAGAAA
  GCCCCAGGGCAGGATGTGACATTGTTTCGCTCTCCAGACTCTGTGGACCGGGTTCTGAGCGAGGGAAGTG
  AAGTATCCAGCGAAGTTCTTAGCGAGCGACGCAACTCCCTTAGGCGGATGTCCTCTTCATTTCCTACCGC
  CGTAGAGACATTCCACCTCATGGGCGAGCTCTCCAGCCTGTATGTTGGCCTCAAGGAATCTATGAAGGAC
  CTGGATGAGGAGCAGGCAGGGCAGACCGACCTTGAGAAAATTCAGTTCCTTCTGGCCCAGATGGTCAAGC
  GCACCATCCCTCCAGAGCTGCAAGAGCAGCTGAAAACGGTTAAAACGCTGGCGAAGGAGGTTTGGCAGGA
  AAAAGCAAAGGTGGAGAGATTGCAGCGAATCCTGGAGGGAGAGGGAAACCAGGAGGCCGGGAAAGAACTT
  AAGGCTGGCGAATTGCGCCTGCAGTTGGGAGTCCTGCGGGTGACAGTCGCCGACATCGAGAAGGAGCTGG
  CGGAGCTGCGGGAATCTCAGGACCGCGGTAAGGCAGCCATGGAAAACAGTGTGAGTGAGGCCTCTCTGTA
  CCTGCAAGACCAGCTTGACAAGCTTCGGATGATCATCGAATCAATGCTGACAAGTAGCTCAACCCTGTTG
  TCCATGTCCATGGCACCGCATAAGGCACACACACTGGCTCCGGGGCAGATCGATCCGGAGGCCACTTGTC
  CAGCCTGCTCACTGGACGTGAGTCACCAGGTAAGTACTCTCGTGCGGAGATACGAACAGCTGCAGGATAT
  GGTTAACTCCCTGGCCGTGTCAAGACCATCCAAAAAAGCAAAATTGCAGCGACAGGACGAAGAACTGCTG
  GGGCGAGTACAAAGTGCAATTCTCCAGGTCCAGGGTGACTGCGAAAAGTTGAATATTACAACTAGTAACC
  TGATAGAGGACCACCGGCAGAAGCAGAAGGATATTGCGATGCTCTACCAGGGACTGGAAAAACTGGAGAA
  GGAGAAGGCCAACCGGGAACACCTGGAGATGGAAATCGATGTCAAGGCAGACAAATCAGCACTTGCTACC
  AAGGTATCACGGGTGCAATTCGATGCCACCACCGAGCAGTTGAACCATATGATGCAAGAGCTGGTAGCCA
  AAATGTCTGGTCAGGAACAAGATTGGCAAAAGATGCTGGACAGACTGCTGACGGAGATGGACAACAAGCT
  CGATCGGCTGGAACTCGATCCCGTCAAGCAGCTCCTCGAGGACCGGTGGAAATCTTTGCGCCAGCAACTG
  CGGGAGCGCCCCCCACTTTACCAGGCGGATGAAGCTGCGGCAATGAGGCGGCAGCTTCTGGCCCACTTTC
  ACTGCCTGTCCTGCGACAGACCACTGGAAACCCCCGTGACGGGACATGCAATCCCAGTGACGCCTGCTGG
  GCCAGGTCTGCCCGGGCACCATTCCATTAGGCCTTATACGGTATTCGAGCTCGAACAGGTCCGGCAGCAT
  AGCCGCAATCTCAAACTGGGTTCCGCTTTCCCACGGGGTGACCTGGCCCAGATGGAGCAGTCAGTAGGAC
  GGCTGCGCAGCATGCATAGCAAAATGCTTATGAACATTGAAAAAGTTCAGATCCACTTTGGGGGCAGCAC
  TAAGGCTTCTAGCCAGATTATCCGAGAGCTCCTTCACGCCCAATGCCTTGGGTCACCCTGCTATAAGCGA
  GTGACCGACATGGCTGATTACACCTACAGTACTGTGCCCAGGCGGTGCGGGGGCTCTCACACCCTTACTT
  ATCCATATCACAGGAGTCGCCCTCAGCACCTCCCAAGAGGACTGTACCCTACTGAGGAGATCCAGATCGC
  TATGAAACACGATGAAGTGGACATCCTCGGCCTGGACGGTCATATCTATAAGGGCAGAATGGACACCAGG
  CTGCCTGGCATACTGAGAAAGGATAGTTCCGGTACTAGTAAGCGCAAAAGCCAGCAACCGCGCCCACATG
  TGCATAGACCTCCTAGTCTGTCCTCCAATGGGCAGCTGCCCAGCCGGCCTCAGTCTGCGCAGATATCCGC
  CGGAAATACTAGTGAGCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212861 representing NM_032134
  Red=Cloning site Green=Tags(s)
  
  MKDAAEELSFARVLLQRVDELEKLFKDREQFLELVSRKLSLVPGAEEVTMVTWEELEQAITDGWRASQAG
  SETLMGFSKHGGFTSLTSPEGTLSGDSTKQPSIEQALDSASGLGPDRTASGSGGTAHPSDGVSSREQSKV
  PSGTGRQQQPRARDEAGVPRLHQSSTFQFKSDSDRHRSREKLTSTQPRRNARPGPVQQDLPLARDQPSSV
  PASQSQVHLRPDRRGLEPTGMNQPGLVPASTYPHGVVPLSMGQLGVPPPEMDDRELIPFVVDEQRMLPPS
  VPGRDQQGLELPSTDQHGLVSVSAYQHGMTFPGTDQRSMEPLGMDQRGCVISGMGQQGLVPPGIDQQGLT
  LPVVDQHGLVLPFTDQHGLVSPGLMPISADQQGFVQPSLEATGFIQPGTEQHDLIQSGRFQRALVQRGAY
  QPGLVQPGADQRGLVRPGMDQSGLAQPGADQRGLVWPGMDQSGLAQPGRDQHGLIQPGTGQHDLVQSGTG
  QGVLVQPGVDQPGMVQPGRFQRALVQPGAYQPGLVQPGADQIDVVQPGADQHGLVQSGADQSDLAQPGAV
  QHGLVQPGVDQRGLAQPRADHQRGLVPPGADQRGLVQPGADQHGLVQPGVDQHGLAQPGEVQRSLVQPGI
  VQRGLVQPGAVQRGLVQPGAVQRGLVQPGVDQRGLVQPGAVQRGLVQPGAVQHGLVQPGADQRGLVQPGV
  DQRGLVQPGVDQRGLVQPGMDQRGLIQPGADQPGLVQPGAGQLGMVQPGIGQQGMVQPQADPHGLVQPGA
  YPLGLVQPGAYLHDLSQSGTYPRGLVQPGMDQYGLRQPGAYQPGLIAPGTKLRGSSTFQADSTGFISVRP
  YQHGMVPPGREQYGQVSPLLASQGLASPGIDRRSLVPPETYQQGLMHPGTDQHSPIPLSTGLGSTHPDQQ
  HVASPGPGEHDQVYPDAAQHGHAFSLFDSHDSMYPGYRGPGYLSADQHGQEGLDPNRTRASDRHGIPAQK
  APGQDVTLFRSPDSVDRVLSEGSEVSSEVLSERRNSLRRMSSSFPTAVETFHLMGELSSLYVGLKESMKD
  LDEEQAGQTDLEKIQFLLAQMVKRTIPPELQEQLKTVKTLAKEVWQEKAKVERLQRILEGEGNQEAGKEL
  KAGELRLQLGVLRVTVADIEKELAELRESQDRGKAAMENSVSEASLYLQDQLDKLRMIIESMLTSSSTLL
  SMSMAPHKAHTLAPGQIDPEATCPACSLDVSHQVSTLVRRYEQLQDMVNSLAVSRPSKKAKLQRQDEELL
  GRVQSAILQVQGDCEKLNITTSNLIEDHRQKQKDIAMLYQGLEKLEKEKANREHLEMEIDVKADKSALAT
  KVSRVQFDATTEQLNHMMQELVAKMSGQEQDWQKMLDRLLTEMDNKLDRLELDPVKQLLEDRWKSLRQQL
  RERPPLYQADEAAAMRRQLLAHFHCLSCDRPLETPVTGHAIPVTPAGPGLPGHHSIRPYTVFELEQVRQH
  SRNLKLGSAFPRGDLAQMEQSVGRLRSMHSKMLMNIEKVQIHFGGSTKASSQIIRELLHAQCLGSPCYKR
  VTDMADYTYSTVPRRCGGSHTLTYPYHRSRPQHLPRGLYPTEEIQIAMKHDEVDILGLDGHIYKGRMDTR
  LPGILRKDSSGTSKRKSQQPRPHVHRPPSLSSNGQLPSRPQSAQISAGNTSER
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032134
• ORF Size: 4989 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032134.1, NM_032134.2, NP_115510.1
• RefSeq Size: 5411 bp
• RefSeq ORF: 4992 bp
• Locus ID: 84074
• UniProt ID: Q9H0J4
• Cytogenetics: 17q25.1
• MW: 180.8 kDa
• Gene Summary: Has an essential role in the formation of sperm flagella and flagellar structure maintainance. It acts as a suppressor of ubiquitination and degradation of proteins involved in flagellar development and motility.[UniProtKB/Swiss-Prot Function]