CYP26A1 (NM_057157) Human Tagged ORF Clone

CAT#: RC212855

CYP26A1 (Myc-DDK-tagged)-Human cytochrome P450, family 26, subfamily A, polypeptide 1 (CYP26A1), transcript variant 2

Product Images

Circular map for RC212855

• 

  Western blot validation of overexpression lysate (Cat# LY409260) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212855 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CYP26A1
• Synonyms: CP26; CYP26; P450RAI; P450RAI1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212855 representing NM_057157
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGCAGGAAATACGGCTTCATCTACAAGACGCATCTGTTCGGGCGGCCCACCGTACGGGTGATGG
  GCGCGGACAATGTGCGGCGCATCTTGCTCGGAGAGCACCGGCTGGTGTCGGTCCACTGGCCAGCGTCGGT
  GCGCACCATTCTGGGATCTGGCTGCCTCTCTAACCTGCACGACTCCTCGCACAAGCAGCGCAAGAAGGTG
  ATTATGCGGGCCTTCAGCCGCGAGGCACTCGAATGCTACGTGCCGGTGATCACCGAGGAAGTGGGCAGCA
  GCCTGGAGCAGTGGCTGAGCTGCGGCGAGCGCGGCCTCCTGGTCTACCCCGAGGTGAAGCGCCTCATGTT
  CCGAATCGCCATGCGCATCCTACTGGGCTGCGAACCCCAACTGGCGGGCGACGGGGACTCCGAGCAGCAG
  CTTGTGGAGGCCTTCGAGGAAATGACCCGCAATCTCTTCTCGCTGCCCATCGACGTGCCCTTCAGCGGGC
  TGTACCGGGGCATGAAGGCGCGGAACCTCATTCACGCGCGCATCGAGCAGAACATTCGCGCCAAGATCTG
  CGGGCTGCGGGCATCCGAGGCGGGCCAGGGCTGCAAAGACGCGCTGCAGCTGTTGATCGAGCACTCGTGG
  GAGAGGGGAGAGCGGCTGGACATGCAGGCACTAAAGCAATCTTCAACCGAACTCCTCTTTGGAGGACACG
  AAACCACGGCCAGTGCAGCCACATCTCTGATCACTTACCTGGGGCTCTACCCACATGTTCTCCAGAAAGT
  GCGAGAAGAGCTGAAGAGTAAGGGTTTACTTTGCAAGAGCAATCAAGACAACAAGTTGGACATGGAAATT
  TTGGAACAACTTAAATACATCGGGTGTGTTATTAAGGAGACCCTTCGACTGAATCCCCCAGTTCCAGGAG
  GGTTTCGGGTTGCTCTGAAGACTTTTGAATTAAATGGATACCAGATTCCCAAGGGCTGGAATGTTATCTA
  CAGTATCTGTGATACTCATGATGTGGCAGAGATCTTCACCAACAAGGAAGAATTTAATCCTGACCGATTC
  ATGCTGCCTCACCCAGAGGATGCATCCAGGTTCAGCTTCATTCCATTTGGAGGAGGCCTTAGGAGCTGTG
  TAGGCAAAGAATTTGCAAAAATTCTTCTCAAAATATTTACAGTGGAGCTGGCCAGGCATTGTGACTGGCA
  GCTTCTAAATGGACCTCCTACAATGAAAACCAGTCCCACCGTGTATCCTGTGGACAATCTCCCTGCAAGA
  TTCACCCATTTCCATGGGGAAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212855 representing NM_057157
  Red=Cloning site Green=Tags(s)
  
  MKRRKYGFIYKTHLFGRPTVRVMGADNVRRILLGEHRLVSVHWPASVRTILGSGCLSNLHDSSHKQRKKV
  IMRAFSREALECYVPVITEEVGSSLEQWLSCGERGLLVYPEVKRLMFRIAMRILLGCEPQLAGDGDSEQQ
  LVEAFEEMTRNLFSLPIDVPFSGLYRGMKARNLIHARIEQNIRAKICGLRASEAGQGCKDALQLLIEHSW
  ERGERLDMQALKQSSTELLFGGHETTASAATSLITYLGLYPHVLQKVREELKSKGLLCKSNQDNKLDMEI
  LEQLKYIGCVIKETLRLNPPVPGGFRVALKTFELNGYQIPKGWNVIYSICDTHDVAEIFTNKEEFNPDRF
  MLPHPEDASRFSFIPFGGGLRSCVGKEFAKILLKIFTVELARHCDWQLLNGPPTMKTSPTVYPVDNLPAR
  FTHFHGEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_057157
• ORF Size: 1284 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_057157.2, NP_476498.1
• RefSeq Size: 2246 bp
• RefSeq ORF: 1287 bp
• Locus ID: 1592
• UniProt ID: O43174
• Cytogenetics: 10q23.33
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Retinol metabolism
• MW: 48.4 kDa
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in regulation of gene expression in both embryonic and adult tissues. Two alternatively spliced transcript variants of this gene, which encode the distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]