KRTAP27-1 (NM_001077711) Human Tagged ORF Clone

CAT#: RC212779

KRTAP27 (Myc-DDK-tagged)-Human keratin associated protein 27-1 (KRTAP27-1)

Product Images

Circular map for RC212779

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  Western blot validation of overexpression lysate (Cat# LY421490) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212779 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KRTAP27-1 protein (Cat# TP312779). The protein was produced from HEK293T cells transfected with KRTAP27-1 cDNA clone (Cat# RC212779) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KRTAP27-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212779 representing NM_001077711
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCATAGCCACTGCCATTCACTCAGGAGCTTCCACAATGCCCCACCACTCTCTGCCATCACACATG
  GCACTAATCCTATAACCTTTGAAGACAGATTGTGTTTGCCCAGCAGCTTCCATAGCAGAACCTGTTTCCT
  GGACAACTTTCAAGAAACCTGCAATGAAACCACCAGCTGCCAAATGACCAATTGTGAACAGGACTTATTC
  ACAGACGATAGCTGTGTGCAAAGTAACTGCTTCCCCGGAGTTGTCCAAACTACTTACTCCAATTCCAGGC
  CCTGCGAAAGGACAGCGTGCCAATCAGAAAGTTCTTCAGCAGGGCTGGCTTGTGTTTCTCAGCCTTGCCA
  ATCAGAAAGCACTCAGCAGATGGGTTTTGTAGCCCAGAGCTGCCAACCTGCAAGCCTCAAGGGAAACAGT
  TGCCCACCCAAGACTTCTAAGTCTAAAAATTTCGAAACTCTGGAACGTGCATCTAGCCAATGTCAGTGTC
  AGTCTCAGAACCCTGAATCCAGTTCCTGTAGACCTCTGGTCAATGTTGCACCTGAGCCACAACTCCTGGA
  ATCTTCTCCAGGAGTTGAACCAACTTGCTGTGTTACTGGTGGTTCTCAATTGCCTAGTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212779 representing NM_001077711
  Red=Cloning site Green=Tags(s)
  
  MPHSHCHSLRSFHNAPPLSAITHGTNPITFEDRLCLPSSFHSRTCFLDNFQETCNETTSCQMTNCEQDLF
  TDDSCVQSNCFPGVVQTTYSNSRPCERTACQSESSSAGLACVSQPCQSESTQQMGFVAQSCQPASLKGNS
  CPPKTSKSKNFETLERASSQCQCQSQNPESSSCRPLVNVAPEPQLLESSPGVEPTCCVTGGSQLPSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077711
• ORF Size: 621 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077711.1, NP_001071179.1
• RefSeq Size: 682 bp
• RefSeq ORF: 624 bp
• Locus ID: 643812
• UniProt ID: Q3LI81
• Cytogenetics: 21q22.11
• MW: 22.2 kDa
• Gene Summary: In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a rigid and resistant hair shaft through their extensive disulfide bond cross-linking with abundant cysteine residues of hair keratins. The matrix proteins include the high-sulfur and high-glycine-tyrosine keratins.[UniProtKB/Swiss-Prot Function]