PCDH8 (NM_032949) Human Tagged ORF Clone

CAT#: RC212755

PCDH8 (Myc-DDK-tagged)-Human protocadherin 8 (PCDH8), transcript variant 2

Product Images

Circular map for RC212755

• 

  Western blot validation of overexpression lysate (Cat# LY409835) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212755 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCDH8
• Synonyms: ARCADLIN; PAPC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212755 representing NM_032949
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCCTGTGAGGCGTTGGGGCAGCCCCTGCCTTTTCCCCTTGCAGCTCTTCAGCCTCTGCTGGGTGC
  TCTCAGTGGCCCAGAGCAAAACAGTCCGATACAGCACCTTCGAGGAGGATGCCCCCGGCACGGTCATCGG
  GACCCTGGCCGAGGACCTGCATATGAAAGTATCGGGTGACACAAGCTTCCGCCTGATGAAGCAATTCAAC
  AGCTCTCTGCTCCGGGTGCGCGAAGGCGACGGGCAGCTGACCGTCGGGGACGCCGGCCTGGACCGCGAGC
  GGCTGTGTGGCCAGGCCCCGCAGTGCGTGCTGGCCTTCGATGTGGTCAGCTTCTCGCAGGAGCAGTTCCG
  GCTGGTGCACGTGGAGGTAGAGGTGAGGGACGTCAACGACCACGCGCCGCGCTTCCCCAGGGCCCAGATC
  CCGGTAGAGGTGTCCGAGGGTGCGGCAGTGGGCACGCGCATCCCCTTGGAGGTGCCGGTGGACGAGGACG
  TGGGCGCCAACGGGCTGCAGACCGTGCGCCTGGCCGAGCCGCACAGCCCCTTTCGCGTGGAGCTGCAGAC
  GCGAGCGGACGGCGCTCAGTGCGCAGACCTGGTGCTGCTGCAGGAGCTGGACCGCGAGAGCCAGGCCGCC
  TACAGCCTGGAGCTGGTGGCCCAGGACGGCGGCCGCCCGCCGCGCTCCGCCACGGCTGCCCTCAGCGTGC
  GCGTCCTGGATGCGAATGACCACAGCCCGGCCTTCCCGCAGGGCGCCGTGGCCGAAGTGGAGCTGGCGGA
  AGACGCGCCCGTGGGCTCCCTGCTTCTCGACCTGGACGCAGCCGACCCCGACGAGGGACCTAACGGCGAC
  GTGGTGTTCGCATTTGGCGCCCGCACCCCGCCGGAGGCGCGCCGCCTCTTTCGGCTTGACCCGCGATCAG
  GCCGCCTCACCCTGGCCGGGCCCGTGGACTACGAGCGTCAGGACACCTACGAGCTGGACGTGCGGGCGCA
  GGACCGCGGACCCGGGCCCCGCGCTGCCACCTGCAAGGTCATCGTGCGCATCCGAGACGTCAATGACAAC
  GCACCCGACATCGCCATCACCCCGCTGGCCGCCCCAGGCGCGCCGGCAACCTCACCCTTCGCCGCTGCCG
  CCGCCGCCGCTGCACTCGGGGGAGCGGACGCTAGCTCGCCGGCGGGAGCCGGGACGCCGGAGGCTGGTGC
  CACTTCGCTGGTGCCGGAGGGGGCGGCGCGCGAGAGCCTGGTGGCCCTGGTCAGCACCTCGGACAGGGAC
  TCGGGCGCCAACGGGCAAGTGCGCTGCGCCCTCTATGGGCACGAGCACTTCCGGCTGCAGCCGGCCTACG
  CGGGCAGCTACCTGGTGGTGACCGCGGCGTCGCTGGACCGCGAACGCATCGCCGAGTACAACTTGACGCT
  GGTGGCCGAGGATCGCGGCGCGCCCCCGCTGCGCACAGTGCGGCCCTACACGGTGCGTGTGGGCGACGAG
  AACGACAACGCGCCGCTCTTCACGCGGCCGGTCTATGAGGTGTCGGTGCGCGAGAACAACCCGCCAGGCG
  CCTACCTGGCCACGGTGGCCGCCCGCGACCGGGACCTGGGCCGCAACGGCCAGGTCACCTACCGGCTGCT
  GGAGGCCGAGGTGGGCCGCGCCGGGGGCGCCGTGTCCACTTATGTCTCGGTGGACCCAGCTACCGGAGCC
  ATCTACGCGCTGCGCAGCTTCGACTATGAGACGCTGCGCCAACTCGACGTTCGCATCCAAGCTAGCGACG
  GCGGCTCCCCTCAGCTTTCCAGCAGCGCCCTAGTGCAAGTGCGCGTGCTGGACCAGAACGACCATGCGCC
  AGTCCTGGTGCACCCGGCGCCAGCCAATGGCTCCCTAGAAGTGGCGGTGCCTGGGCGCACCGCAAAGGAC
  ACGGTTGTGGCCCGTGTGCAGGCCCGGGATGCAGACGAGGGAGCCAACGGGGAGCTGGCGTTCGAGCTGC
  AGCAGCAGGAGCCGCGCGAAGCCTTCGCCATCGGCCGCCGCACGGGGGAGATACTGCTCACCGGCGACCT
  CTCGCAGGAGCCACCCGGTCGCGTGTTCAGGGCGCTCCTGGTCATATCCGACGGCGGCCGTCCCCCGCTC
  ACCACCACCGCAACTGTCAGCTTCGTGGTAACAGCAGGGGGCGGGCGTGGGCCGGCTGCGCCTGCCAGTG
  CAGGAAGCCCGGAGCGTTCCCGCCCGCCTGGCTCTCGGCTCGGGGTGTCCGGGTCGGTGCTGCAATGGGA
  CACGCCGCTGATCGTCATCATCGTGCTGGCCGGGAGCTGCACGCTGCTGCTGGCCGCCATCATCGCCATC
  GCCACCACCTGCAACCGCCGCAAGAAGGAGCCCTACGGTGCCTCCCCGGGTTTTGGAAAGGAGCCGGCGC
  CCCCTGTGGCGGTGTGGAAAGGACACTCCTTCAACACCATTTCTGGCAGAGAAGCAGAGAAGTTCAGCGG
  CAAAGACAGCGGTAAAGGGGACAGTGATTTCAACGACAGCGATTCCGACATCAGCGGGGACGCTCTGAAA
  AAGGATCTCATCAACCACATGCAGAGTGGACTGTGGGCGTGCACCGCTGAGTGTAAGATCCTGGGCCACT
  CTGACCGCTGCTGGAGCCCATCCTGCAGCGGGCCCAACGCACATCCATCGCCTCACCCACCAGCCCAGAT
  GTCAACCTTCTGTAAGAGCACGTCACTGCCTCGGGATCCTCTGCGCAGGGACAATTACTACCAGGCCCAG
  CTGCCCAAGACAGTGGGGCTGCAGAGCGTCTATGAGAAAGTACTGCACAGAGACTATGACAGGACAGTCA
  CTCTGCTCTCCCCTCCCCGTCCAGGGAGGCTCCCAGACCTGCAGGAGATTGGAGTACCCCTCTACCAGTC
  CCCTCCTGGCAGGTACCTGTCCCCGAAGAAGGGAGCCAATGAAAATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212755 representing NM_032949
  Red=Cloning site Green=Tags(s)
  
  MSPVRRWGSPCLFPLQLFSLCWVLSVAQSKTVRYSTFEEDAPGTVIGTLAEDLHMKVSGDTSFRLMKQFN
  SSLLRVREGDGQLTVGDAGLDRERLCGQAPQCVLAFDVVSFSQEQFRLVHVEVEVRDVNDHAPRFPRAQI
  PVEVSEGAAVGTRIPLEVPVDEDVGANGLQTVRLAEPHSPFRVELQTRADGAQCADLVLLQELDRESQAA
  YSLELVAQDGGRPPRSATAALSVRVLDANDHSPAFPQGAVAEVELAEDAPVGSLLLDLDAADPDEGPNGD
  VVFAFGARTPPEARRLFRLDPRSGRLTLAGPVDYERQDTYELDVRAQDRGPGPRAATCKVIVRIRDVNDN
  APDIAITPLAAPGAPATSPFAAAAAAAALGGADASSPAGAGTPEAGATSLVPEGAARESLVALVSTSDRD
  SGANGQVRCALYGHEHFRLQPAYAGSYLVVTAASLDRERIAEYNLTLVAEDRGAPPLRTVRPYTVRVGDE
  NDNAPLFTRPVYEVSVRENNPPGAYLATVAARDRDLGRNGQVTYRLLEAEVGRAGGAVSTYVSVDPATGA
  IYALRSFDYETLRQLDVRIQASDGGSPQLSSSALVQVRVLDQNDHAPVLVHPAPANGSLEVAVPGRTAKD
  TVVARVQARDADEGANGELAFELQQQEPREAFAIGRRTGEILLTGDLSQEPPGRVFRALLVISDGGRPPL
  TTTATVSFVVTAGGGRGPAAPASAGSPERSRPPGSRLGVSGSVLQWDTPLIVIIVLAGSCTLLLAAIIAI
  ATTCNRRKKEPYGASPGFGKEPAPPVAVWKGHSFNTISGREAEKFSGKDSGKGDSDFNDSDSDISGDALK
  KDLINHMQSGLWACTAECKILGHSDRCWSPSCSGPNAHPSPHPPAQMSTFCKSTSLPRDPLRRDNYYQAQ
  LPKTVGLQSVYEKVLHRDYDRTVTLLSPPRPGRLPDLQEIGVPLYQSPPGRYLSPKKGANENV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032949
• ORF Size: 2919 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032949.3
• RefSeq Size: 3785 bp
• RefSeq ORF: 2922 bp
• Locus ID: 5100
• UniProt ID: O95206
• Cytogenetics: 13q14.3
• Domains: CA
• Protein Families: Transmembrane
• MW: 100.5 kDa
• Gene Summary: This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The gene encodes an integral membrane protein that is thought to function in cell adhesion in a CNS-specific manner. Unlike classical cadherins, which are generally encoded by 15-17 exons, this gene includes only 3 exons. Notable is the large first exon encoding the extracellular region, including 6 cadherin domains and a transmembrane region. Alternative splicing yields isoforms with unique cytoplasmic tails. [provided by RefSeq, Jul 2008]