ANKRD56 (SOWAHB) (NM_001029870) Human Tagged ORF Clone

CAT#: RC212470

SOWAHB (Myc-DDK-tagged)-Human ankyrin repeat domain 56 (ANKRD56)

Product Images

Circular map for RC212470

• 

  Western blot validation of overexpression lysate (Cat# LY422254) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212470 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANKRD56
• Synonyms: ANKRD56
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212470 representing NM_001029870
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGAGAGCTGAGCCAGGAGGCACTACTGGACTTTCTGTGCCAGGCTGGGGGCCGCGTGACCAACG
  CTGCCTTGCTGAGCCACTTCAAGAGCTTTCTCCGAGACCCCGACGCGTCCCCCAGCCAGCACCAGCACCG
  CCGCGAGCTCTTCAAGGGCTTCGTCAACTCGGTCGCCGCAGTGCGCCAGGACCCCGACGGCACCAAGTAC
  GTGGTGCTCAAGAGGAGATACAGGGACCTTTTGGGGGAGGAGGGGCTGCAGCGACCCCGCGAGCCGCCCG
  CGGCCGCCCCCAGTGCAGGGGGAGCTGCGCCCTGCTCCCCGCGAGGCGCGCGCCGGGGGGAGCCGCCCCA
  GCAGCAGCCCAGGCGGCGGCGGCGCGAGAAGGAGCCGGAGGAGGAGCCAGCAGGTGCAGCAGCCAGAGCC
  GCCGACGCAGCTTGCAATGGACTCCCGGGCAGCGACTCCCGTAGGGCGCCCGGGAAGGGCGGCGGATCGA
  AGGGCAGTCCCGGACAGAGGCCGCCGGTGCCCGCAGCTGCAGCGGCAGGGGCCCAGGCGAGAGCGAGCTG
  CGCGGCGGCGAAGACGCAGGGCCGCTGCTGCTGGGAATGCCTCCAGAACAACCTGGCTGTACTGCCGGGA
  GAGCTCGGCGCACTCCCGCACTCGGCCACCGCGGAGGAGAAGCCGGCACGGGCTCTGCCTGCCCAGGATG
  ACCGCGGGGCTTCCAGGGAGCGGGAAGAAGGCGCGCTAGCTGAGCCGGCGCCTGTGCCTGCAGTGGCTCA
  CTCGCCTCCCGCCACCGTCGAGGCTGCGACAAGCAGGGCTTCCCCGCCTGCTCTCCTGCCCGGCCCCGCT
  CCCCGCGGAGACCGGCCGGAGCTGCTGACCCCCAGCTCCCTGCATTATTCGACCCTGCAGCAGCAGCAGC
  AGCGCACTCGAGAGTGGGTGGCCAGGCACCCGCAGGTGCCCGAGGCCCGTGATCAGGGCCCTATCCGCGC
  CTGGTCGGTGCTGCCAGACAACTTCCTCCAGCTGCCCTTGGAACCCGGCTCCACGGAGCCTAATTCAGAG
  CCGCCAGACCCCTGTCTTTCCTCGCACTCTCTCTTTCCTGTTGTTCCGGATGAGTCCTGGGAATCCTGGG
  CGGGGAACCCTTCATTGACTGTCTTTCGCAGCATTCGTTGTCAGCTGTCCCTCCAAGATCTGGATGACTT
  TGTGGACCAGGAGAGTGATGGCAGTGAGGAGAGCAGCAGTGGGCCCAAAGACTCCCCGGGGGCTTCTGAA
  GAGGGGCTGCAGGTTGTCTTGGGAACCCCAGATAGGGGGAAGCTCAGGAATCCAGCTGGGGGCCTTTCTG
  TATCTCGGAAGGAGGGCAGCCCCAGCCGGAGCCCTCAGGGTCTCAGAAACAGAGGGGATGGTCACATCTC
  TCAGCAGGTCCCTGCAGGGGCTAATGGCCTTGCAGGCCACCCCCTGAAGCCTTTGCCTTGGCCAGTTCCT
  AAGTTAAGGAGGTCCCTCAGGAGGAGCTCTCTGGCAGGGAGAGCCAAATTGTCCTCCTCTGATGAGGAGT
  ACCTCGATGAGGGCTTGCTGAAAAGAAGTCGGCGCCCACCTCGATCCAGGAAGCCCTCCAAGGCAGGAAC
  GGCACCCAGCCCAAGGGTTGATGCAGGTTTATCACTAAAACTTGCAGAGGTTAAGGCTGTTGTGGCCGAG
  CGGGGTTGGCGACACAGCCTGTGGGTCCCCAGTGGGGAGGGGTCTGCAGCCTTGGCCCCCCACAGAACTT
  CTGAGCACAAATCATCCCTGGTTCCACTAGATGCCAGGGAGCATGAGTGGATTGTGAAGCTTGCCAGTGG
  CTCCTGGATTCAGGTGTGGACTTTGTTCTGGGAGGACCCTCAACTGGCCTTGCACAAAGACTTTTTGACT
  GGGTACACTGCGTTGCACTGGATAGCCAAACATGGTGACCTCAGGGCCCTTCAGGACTTGGTGTCTGGAG
  CAAAGAAGGCAGGGATTGTCCTTGATGTAAACGTGAGGTCCAGTTGTGGATATACCCCGCTGCACCTTGC
  AGCCATTCACGGCCACCAGGGGGTCATCAAATTGCTAGTGCAAAGGTTGGCTTCTCGGGTAAATGTCAGG
  GACAGCAGTGGGAAGAAGCCATGGCAGTATCTAACCAGTAATACCTCTGGGGAAATATGGCAGCTGTTGG
  GAGCTCCTCGGGGCAAGCCCATTTTCCCTGTCTATCCCTTAGTTGGAAGTTCTTCCCCTACCAGAAAGGC
  CAAGAGCAAGGAAATATCTAGAAGTGTCACCCGAAAAACTTCCTTCGCTGCACTACTCAAAAGTCAGCAC
  AACAAGTGGAAACTGGCCAACCAGTATGAGAAATTCCACAGTCCAAGGGAAAGAGAAGAGTATAGTGAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212470 representing NM_001029870
  Red=Cloning site Green=Tags(s)
  
  MARELSQEALLDFLCQAGGRVTNAALLSHFKSFLRDPDASPSQHQHRRELFKGFVNSVAAVRQDPDGTKY
  VVLKRRYRDLLGEEGLQRPREPPAAAPSAGGAAPCSPRGARRGEPPQQQPRRRRREKEPEEEPAGAAARA
  ADAACNGLPGSDSRRAPGKGGGSKGSPGQRPPVPAAAAAGAQARASCAAAKTQGRCCWECLQNNLAVLPG
  ELGALPHSATAEEKPARALPAQDDRGASREREEGALAEPAPVPAVAHSPPATVEAATSRASPPALLPGPA
  PRGDRPELLTPSSLHYSTLQQQQQRTREWVARHPQVPEARDQGPIRAWSVLPDNFLQLPLEPGSTEPNSE
  PPDPCLSSHSLFPVVPDESWESWAGNPSLTVFRSIRCQLSLQDLDDFVDQESDGSEESSSGPKDSPGASE
  EGLQVVLGTPDRGKLRNPAGGLSVSRKEGSPSRSPQGLRNRGDGHISQQVPAGANGLAGHPLKPLPWPVP
  KLRRSLRRSSLAGRAKLSSSDEEYLDEGLLKRSRRPPRSRKPSKAGTAPSPRVDAGLSLKLAEVKAVVAE
  RGWRHSLWVPSGEGSAALAPHRTSEHKSSLVPLDAREHEWIVKLASGSWIQVWTLFWEDPQLALHKDFLT
  GYTALHWIAKHGDLRALQDLVSGAKKAGIVLDVNVRSSCGYTPLHLAAIHGHQGVIKLLVQRLASRVNVR
  DSSGKKPWQYLTSNTSGEIWQLLGAPRGKPIFPVYPLVGSSSPTRKAKSKEISRSVTRKTSFAALLKSQH
  NKWKLANQYEKFHSPREREEYSD
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001029870
• ORF Size: 2379 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001029870.3
• RefSeq Size: 2921 bp
• RefSeq ORF: 2382 bp
• Locus ID: 345079
• UniProt ID: A6NEL2
• Cytogenetics: 4q21.1
• MW: 85.6 kDa