ADAMTS8 (NM_007037) Human Tagged ORF Clone

CAT#: RC212446

ADAMTS8 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 8 (ADAMTS8)

Product Images

Circular map for RC212446

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ADAMTS8 (Cat# RC212446, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADAMTS8(Cat# TA505684). Positive lysates LY402079 (100ug) and LC402079 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY402079) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212446 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ADAMTS8 protein (Cat# TP312446). The protein was produced from HEK293T cells transfected with ADAMTS8 cDNA clone (Cat# RC212446) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADAMTS8
• Synonyms: ADAM-TS8; METH2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212446 representing NM_007037
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCCCGCCCCCGCCGCCCCCCGGTGGCCTCCGCTCCTGCTGCTGCTGCTGCTGCTGCCGCTGGCCC
  GCGGCGCCCCGGCCCGGCCCGCAGCCGGGGGGCAGGCCTCGGAGCTGGTGGTGCCCACGCGGTTGCCCGG
  CAGCGCGGGCGAGCTCGCGCTCCACCTGTCCGCCTTCGGCAAGGGCTTCGTGCTGCGCCTGGCGCCCGAC
  GACAGCTTCCTGGCGCCCGACTTCAAGATCGAGCGCCTCGGGGGCTCCGGCCGGGCGACCGGGGGCGAGC
  GGGGGCTGCGCGGCTGCTTCTTCTCCGGCACCGTGAATGGGGAGCCCGAGTCGCTGGCGGCGGTCAGCCT
  GTGCCGCGGGCTGAGCGGCTCCTTCCTGCTGGACGGCGAGGAGTTCACCATCCAGCCGCAGGGCGCGGGG
  GGCTCCCTGGCTCAGCCGCACCGCCTGCAGCGCTGGGGTCCCGCCGGAGCCCGCCCCCTCCCGCGAGGAC
  CCGAGTGGGAGGTGGAGACGGGAGAGGGTCAGAGGCAGGAGAGAGGAGACCACCAGGAGGACAGCGAGGA
  GGAGAGCCAAGAAGAGGAGGCAGAAGGCGCTAGCGAGCCGCCACCGCCCCTGGGGGCCACGAGTAGGACC
  AAGCGGTTTGTGTCTGAGGCGCGCTTCGTGGAGACGCTGCTGGTGGCCGATGCGTCCATGGCTGCCTTCT
  ACGGGGCCGACCTGCAGAACCACATCCTGACGTTAATGTCTGTGGCAGCCCGAATCTACAAGCACCCCAG
  CATCAAGAATTCCATCAACCTGATGGTGGTAAAAGTGCTGATCGTAGAAGATGAAAAATGGGGCCCAGAG
  GTGTCCGACAATGGGGGGCTTACACTGCGTAACTTCTGCAACTGGCAGCGGCGTTTCAACCAGCCCAGCG
  ACCGCCACCCAGAGCACTACGACACGGCCATCCTGCTCACCAGACAGAACTTCTGTGGGCAGGAGGGGCT
  GTGTGACACCCTGGGTGTGGCAGACATCGGGACCATTTGTGACCCCAACAAAAGCTGCTCCGTGATCGAG
  GATGAGGGGCTCCAGGCGGCCCACACCCTGGCCCATGAACTAGGGCACGTCCTCAGCATGCCCCACGACG
  ACTCCAAGCCCTGCACACGGCTCTTCGGGCCCATGGGCAAGCACCACGTGATGGCACCGCTGTTCGTCCA
  CCTGAACCAGACGCTGCCCTGGTCCCCCTGCAGCGCCATGTATCTCACAGAGCTTCTGGACGGCGGGCAC
  GGAGACTGTCTCCTGGATGCCCCTGCTGCGGCCCTGCCCCTCCCCACAGGCCTCCCGGGCCGCATGGCCC
  TGTACCAGCTGGACCAGCAGTGCAGGCAGATCTTTGGGCCGGATTTCCGCCACTGCCCCAACACCTCTGC
  TCAGGACGTCTGCGCCCAGCTTTGGTGCCACACTGATGGGGCTGAGCCCCTGTGCCACACGAAGAATGGC
  AGCCTGCCCTGGGCTGACGGCACGCCGTGCGGGCCTGGGCACCTCTGCTCAGAAGGCAGCTGTCTACCTG
  AGGAGGAAGTGGAGAGGCCCAAGCCCGTGGTAGATGGAGGCTGGGCACCGTGGGGACCCTGGGGAGAATG
  TTCTCGGACCTGTGGAGGAGGAGTACAGTTTTCACACCGTGAGTGCAAGGACCCCGAGCCTCAGAATGGA
  GGAAGATACTGCCTGGGTCGGAGAGCCAAGTACCAGTCATGCCACACGGAGGAATGCCCCCCTGACGGGA
  AAAGCTTCAGGGAGCAGCAGTGTGAGAAGTATAATGCCTACAATTACACTGACATGGACGGGAATCTCCT
  GCAGTGGGTCCCCAAGTATGCTGGGGTGTCCCCCCGGGACCGCTGCAAGTTGTTCTGCCGAGCCCGGGGG
  AGGAGCGAGTTCAAAGTGTTCGAGGCCAAGGTGATTGATGGCACCCTGTGTGGGCCAGAAACACTGGCCA
  TCTGTGTCCGTGGCCAGTGTGTCAAGGCCGGCTGTGACCATGTGGTGGACTCGCCTCGGAAGCTGGACAA
  ATGCGGGGTGTGTGGGGGCAAAGGCAACTCCTGCAGGAAGGTCTCCGGGTCCCTCACCCCCACCAATTAT
  GGCTACAATGACATTGTCACCATCCCAGCTGGTGCCACTAATATTGACGTGAAGCAGCGGAGCCACCCGG
  GTGTGCAGAACGATGGGAACTACCTGGCGCTGAAGACGGCTGATGGGCAGTACCTGCTCAACGGCAACCT
  GGCCATCTCTGCCATAGAGCAGGACATCTTGGTGAAGGGGACCATCCTGAAGTACAGCGGCTCCATCGCC
  ACCCTGGAGCGCCTGCAGAGCTTCCGGCCCTTGCCAGAGCCTCTGACAGTGCAGCTCCTGACAGTCCCTG
  GCGAGGTCTTCCCCCCAAAAGTCAAATACACCTTCTTTGTTCCTAATGACGTGGACTTTAGCATGCAGAG
  CAGCAAAGAGAGAGCAACCACCAACATCATCCAGCCGCTGCTCCACGCACAGTGGGTGCTGGGGGACTGG
  TCTGAGTGCTCTAGCACCTGCGGGGCCGGCTGGCAGAGGCGAACTGTAGAGTGCAGGGACCCCTCCGGCC
  AGGCCTCTGCCACCTGCAACAAGGCTCTGAAACCCGAGGATGCCAAGCCCTGCGAAAGCCAGCTGTGCCC
  CCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212446 representing NM_007037
  Red=Cloning site Green=Tags(s)
  
  MLPAPAAPRWPPLLLLLLLLPLARGAPARPAAGGQASELVVPTRLPGSAGELALHLSAFGKGFVLRLAPD
  DSFLAPDFKIERLGGSGRATGGERGLRGCFFSGTVNGEPESLAAVSLCRGLSGSFLLDGEEFTIQPQGAG
  GSLAQPHRLQRWGPAGARPLPRGPEWEVETGEGQRQERGDHQEDSEEESQEEEAEGASEPPPPLGATSRT
  KRFVSEARFVETLLVADASMAAFYGADLQNHILTLMSVAARIYKHPSIKNSINLMVVKVLIVEDEKWGPE
  VSDNGGLTLRNFCNWQRRFNQPSDRHPEHYDTAILLTRQNFCGQEGLCDTLGVADIGTICDPNKSCSVIE
  DEGLQAAHTLAHELGHVLSMPHDDSKPCTRLFGPMGKHHVMAPLFVHLNQTLPWSPCSAMYLTELLDGGH
  GDCLLDAPAAALPLPTGLPGRMALYQLDQQCRQIFGPDFRHCPNTSAQDVCAQLWCHTDGAEPLCHTKNG
  SLPWADGTPCGPGHLCSEGSCLPEEEVERPKPVVDGGWAPWGPWGECSRTCGGGVQFSHRECKDPEPQNG
  GRYCLGRRAKYQSCHTEECPPDGKSFREQQCEKYNAYNYTDMDGNLLQWVPKYAGVSPRDRCKLFCRARG
  RSEFKVFEAKVIDGTLCGPETLAICVRGQCVKAGCDHVVDSPRKLDKCGVCGGKGNSCRKVSGSLTPTNY
  GYNDIVTIPAGATNIDVKQRSHPGVQNDGNYLALKTADGQYLLNGNLAISAIEQDILVKGTILKYSGSIA
  TLERLQSFRPLPEPLTVQLLTVPGEVFPPKVKYTFFVPNDVDFSMQSSKERATTNIIQPLLHAQWVLGDW
  SECSSTCGAGWQRRTVECRDPSGQASATCNKALKPEDAKPCESQLCPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007037
• ORF Size: 2664 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007037.6
• RefSeq Size: 4028 bp
• RefSeq ORF: 2670 bp
• Locus ID: 11095
• UniProt ID: Q9UP79
• Cytogenetics: 11q24.3
• Protein Families: Druggable Genome, Protease, Secreted Protein
• MW: 96.3 kDa
• Gene Summary: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme contains two C-terminal TS motifs, and disrupts angiogenesis in vivo. A number of disorders have been mapped in the vicinity of this gene, most notably lung neoplasms. Reduced expression of this gene has been observed in multiple human cancers and this gene has been proposed as a potential tumor suppressor. [provided by RefSeq, Feb 2016]