RSL1D1 (NM_015659) Human Tagged ORF Clone

CAT#: RC212331

RSL1D1 (Myc-DDK-tagged)-Human ribosomal L1 domain containing 1 (RSL1D1)

Product Images

Circular map for RC212331

• 

  Western blot validation of overexpression lysate (Cat# LY414420) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212331 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RSL1D1
• Synonyms: CSIG; L12; PBK1; UTP30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212331 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGATTCGGCCTCGGCCTCGCTGTCTTCTGCAGCCGCTACTGGAACCTCCACCTCGACTCCAGCGG
  CCCCGACAGCACGGAAGCAGCTGGATAAAGAACAGGTTAGAAAGGCAGTGGACGCTCTCTTGACGCATTG
  CAAGTCCAGGAAAAACAATTATGGGTTGCTTTTGAATGAGAATGAAAGTTTATTTTTAATGGTGGTATTA
  TGGAAAATTCCAAGTAAAGAACTGAGGGTCAGATTGACCTTGCCTCATAGTATTCGATCAGATTCAGAAG
  ATATCTGTTTATTTACGAAGGATGAACCCAATTCAACTCCTGAAAAGACAGAACAGTTTTATAGAAAGCT
  TTTAAACAAGCATGGAATTAAAACCGTTTCTCAGATTATCTCCCTCCAAACTCTAAAGAAGGAATATAAA
  TCCTATGAAGCCAAGCTCCGCCTTCTGAGCAGTTTTGATTTCTTCCTTACTGATGCCAGAATTAGGCGGC
  TCTTACCCTCACTCATTGGGAGACATTTCTATCAAAGAAAGAAAGTTCCAGTATCTGTAAACCTTCTGTC
  CAAGAATTTATCAAGAGAGATCAATGACTGTATAGGTGGAACAGTCTTAAACATTTCTAAAAGTGGTTCT
  TGCAGTGCTATACGTATTGGTCACGTTGGAATGCAAATTGAGCACATCATTGAAAACATTGTTGCTGTCA
  CCAAAGGACTTTCAGAAAAATTGCCAGAGAAGTGGGAGAGCGTGAAACTCCTGTTTGTGAAAACTGAGAA
  ATCGGCTGCACTTCCCATCTTTTCCTCGTTTGTCAGCAATTGGGATGAAGCCACCAAAAGATCTTTGCTT
  AATAAGAAGAAAAAAGAGGCAAGGAGAAAACGAAGAGAAAGAAATTTTGAAAAACAAAAGGAGAGGAAGA
  AGAAGAGGCAGCAGGCTAGGAAGACTGCATCAGTTCTTAGTAAAGATGATGTGGCACCTGAAAGTGGTGA
  TACTACAGTGAAGAAACCTGAATCAAAGAAGGAACAGACCCCAGAGCATGGGAAGAAAAAACGTGGCAGA
  GGAAAAGCCCAAGTTAAAGCAACAAATGAATCCGAAGACGAAATCCCACAGCTGGTACCAATAGGAAAGA
  AGACTCCAGCTAATGAAAAAGTAGAGATTCAAAAACATGCCACAGGAAAGAAGTCTCCAGCAAAGAGTCC
  TAATCCCAGCACACCTCGTGGGAAGAAAAGAAAGGCTTTGCCAGCATCTGAGACCCCAAAAGCTGCAGAG
  TCTGAGACCCCAGGGAAAAGCCCAGAGAAGAAGCCAAAAATCAAAGAAGAGGCAGTGAAGGAAAAAAGTC
  CTTCGCTGGGGAAAAAAGATGCGAGACAGACTCCAAAAAAGCCAGAGGCCAAGTTTTTCACCACTCCTAG
  TAAATCTGTGAGAAAAGCTTCCCACACCCCCAAAAAATGGCCCAAAAAACCCAAAGTACCCCAGTCGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212331 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEDSASASLSSAAATGTSTSTPAAPTARKQLDKEQVRKAVDALLTHCKSRKNNYGLLLNENESLFLMVVL
  WKIPSKELRVRLTLPHSIRSDSEDICLFTKDEPNSTPEKTEQFYRKLLNKHGIKTVSQIISLQTLKKEYK
  SYEAKLRLLSSFDFFLTDARIRRLLPSLIGRHFYQRKKVPVSVNLLSKNLSREINDCIGGTVLNISKSGS
  CSAIRIGHVGMQIEHIIENIVAVTKGLSEKLPEKWESVKLLFVKTEKSAALPIFSSFVSNWDEATKRSLL
  NKKKKEARRKRRERNFEKQKERKKKRQQARKTASVLSKDDVAPESGDTTVKKPESKKEQTPEHGKKKRGR
  GKAQVKATNESEDEIPQLVPIGKKTPANEKVEIQKHATGKKSPAKSPNPSTPRGKKRKALPASETPKAAE
  SETPGKSPEKKPKIKEEAVKEKSPSLGKKDARQTPKKPEAKFFTTPSKSVRKASHTPKKWPKKPKVPQST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015659
• ORF Size: 1470 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015659.3
• RefSeq Size: 5137 bp
• RefSeq ORF: 1473 bp
• Locus ID: 26156
• UniProt ID: O76021
• Cytogenetics: 16p13.13
• MW: 55 kDa
• Gene Summary: Regulates cellular senescence through inhibition of PTEN translation. Acts as a pro-apoptotic regulator in response to DNA damage.[UniProtKB/Swiss-Prot Function]