ENTPD7 (NM_020354) Human Tagged ORF Clone

CAT#: RC212315

ENTPD7 (Myc-DDK-tagged)-Human ectonucleoside triphosphate diphosphohydrolase 7 (ENTPD7)

Product Images

Circular map for RC212315

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  Western blot validation of overexpression lysate (Cat# LY412525) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212315 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ENTPD7 protein (Cat# TP312315). The protein was produced from HEK293T cells transfected with ENTPD7 cDNA clone (Cat# RC212315) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ENTPD7
• Synonyms: LALP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212315 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAGGATCAGTTTTTCCTACCTCTGCCCAGCCTCCTGGTACTTCACTGTGCCCACAGTGAGTCCAT
  TTCTCCGTCAGCGGGTGGCATTCCTGGGACTCTTCTTCATATCCTGTCTCCTTTTACTTATGTTAATCAT
  AGACTTTCGACATTGGAGTGCTTCATTACCACGAGATAGGCAATACGAAAGGTATTTGGCTCGAGTAGGG
  GAGCTTGAAGCTACTGACACTGAAGACCCAAATCTGAATTATGGACTTGTTGTTGACTGTGGCAGCAGTG
  GTTCCCGGATTTTTGTTTATTTCTGGCCAAGACATAATGGGAACCCCCATGACTTGCTGGACATCAAACA
  GATGAGAGACCGCAACAGCCAACCAGTGGTTAAAAAAATCAAGCCAGGAATCTCTGCAATGGCAGACACT
  CCAGAACATGCCAGTGATTACCTTCGTCCTCTGCTGAGCTTTGCTGCTGCTCATGTGCCTGTGAAGAAGC
  ACAAGGAGACCCCTCTTTACATCCTCTGCACAGCAGGCATGAGGCTTCTCCCTGAGAGGAAGCAGTTGGC
  TATCTTGGCTGACCTAGTGAAAGATTTACCACTGGAGTTTGACTTCCTCTTTTCACAGTCTCAAGCAGAA
  GTGATCTCTGGGAAGCAGGAAGGGGTTTATGCATGGATTGGAATCAACTTTGTTTTGGGAAGATTCGACC
  ACGAGGATGAATCAGATGCTGAGGCTACCCAGGAATTGGCAGCAGGACGGAGAAGGACAGTAGGGATACT
  GGATATGGGAGGAGCCTCTCTCCAAATTGCTTATGAAGTTCCTACCTCAACCTCTGTCCTTCCTGCAAAG
  CAGGAAGAAGCTGCCAAGATCCTGCTGGCTGAGTTCAACCTGGGCTGTGATGTGCAACACACTGAACACG
  TGTACAGGGTTTATGTCACAACTTTTCTGGGTTTCGGAGGCAACTTTGCCCGGCAGCGCTACGAAGACCT
  TGTTCTGAATGAAACTCTTAACAAAAACAGATTGCTTGGTCAGAAGACAGGTCTGAGTCCCGACAATCCA
  TTTCTGGATCCCTGCCTGCCAGTGGGACTCACAGATGTGGTGGAGAGGAACAGCCAAGTCTTACATGTCC
  GAGGAAGAGGAGACTGGGTGTCTTGTGGGGCAATGCTGAGCCCCCTGCTGGCTCGCTCCAACACCAGCCA
  GGCCTCACTCAATGGCATATATCAATCGCCTATTGACTTCAACAACAGCGAGTTCTACGGCTTCTCTGAG
  TTTTTTTATTGTACAGAGGATGTGTTGCGCATTGGTGGCCGCTACCATGGGCCAACATTTGCCAAGGCTG
  CTCAGGATTACTGTGGCATGGCTTGGTCGGTACTAACTCAGAGATTCAAGAATGGCCTCTTTTCATCACA
  TGCAGATGAGCATCGACTCAAATATCAGTGTTTTAAATCGGCTTGGATGTACCAAGTCTTACATGAAGGA
  TTCCACTTTCCCTATGACTACCCAAACCTGCGGACAGCCCAGCTGGTGTATGACCGAGAGGTTCAGTGGA
  CGCTGGGAGCCATTCTATATAAAACACGATTCTTACCACTCAGGGATCTTCGGCAGGAAGGTGTCCGACA
  AGCCCATGGTAGCTGGTTCCGTCTCTCCTTTGTATACAACCACTATCTCTTCTTTGCCTGTATCCTGGTG
  GTGCTACTGGCCATCTTCCTATACCTTCTGCGGCTACGCCGAATTCACCACCGACAAACACGAGCCTCAG
  CTCCATTGGACTTGCTGTGGCTTGAAGAGGTGGTGCCCATGATGGGAGTACAGGTGGGGCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212315 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MARISFSYLCPASWYFTVPTVSPFLRQRVAFLGLFFISCLLLLMLIIDFRHWSASLPRDRQYERYLARVG
  ELEATDTEDPNLNYGLVVDCGSSGSRIFVYFWPRHNGNPHDLLDIKQMRDRNSQPVVKKIKPGISAMADT
  PEHASDYLRPLLSFAAAHVPVKKHKETPLYILCTAGMRLLPERKQLAILADLVKDLPLEFDFLFSQSQAE
  VISGKQEGVYAWIGINFVLGRFDHEDESDAEATQELAAGRRRTVGILDMGGASLQIAYEVPTSTSVLPAK
  QEEAAKILLAEFNLGCDVQHTEHVYRVYVTTFLGFGGNFARQRYEDLVLNETLNKNRLLGQKTGLSPDNP
  FLDPCLPVGLTDVVERNSQVLHVRGRGDWVSCGAMLSPLLARSNTSQASLNGIYQSPIDFNNSEFYGFSE
  FFYCTEDVLRIGGRYHGPTFAKAAQDYCGMAWSVLTQRFKNGLFSSHADEHRLKYQCFKSAWMYQVLHEG
  FHFPYDYPNLRTAQLVYDREVQWTLGAILYKTRFLPLRDLRQEGVRQAHGSWFRLSFVYNHYLFFACILV
  VLLAIFLYLLRLRRIHHRQTRASAPLDLLWLEEVVPMMGVQVGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020354
• ORF Size: 1812 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020354.5
• RefSeq Size: 8564 bp
• RefSeq ORF: 1815 bp
• Locus ID: 57089
• UniProt ID: Q9NQZ7
• Cytogenetics: 10q24.2
• Domains: GDA1_CD39
• Protein Families: Transmembrane
• MW: 69 kDa
• Gene Summary: This gene encodes a purine-converting ectoenzyme which belongs to the ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase) family. The encoded protein hydrolyzes extracellular nucleoside triphosphates (UTP, GTP, and CTP) to nucleoside monophosphates as part of a purinergic signaling pathway. It contains two transmembrane domains at the N- and C-termini and a large, hydrophobic catalytic domain located in between. This gene affects oxidative stress as well as DNA damage and is a mediator of senescence. [provided by RefSeq, Mar 2017]