DPS1 (PDSS1) (NM_014317) Human Tagged ORF Clone

CAT#: RC212263

PDSS1 (Myc-DDK-tagged)-Human prenyl (decaprenyl) diphosphate synthase, subunit 1 (PDSS1)

Product Images

Circular map for RC212263

• 

  Western blot validation of overexpression lysate (Cat# LY415361) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212263 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DPS1
• Synonyms: COQ1; COQ1A; COQ10D2; DPS; hDPS1; SPS; TPRT; TPT; TPT 1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212263 representing NM_014317
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGCGCTGGTGGCGGTGGCGGCGCGGCTGCTCCTGGAAGCCGGCGGCGCGGAGCCCCGGGCCCG
  GCTCCCCCGGCCGTGCGGGACCGTTGGGGCCGAGCGCCGCTGCCGAAGTCCGCGCGCAGGTTCATAGGCG
  GAAGGGACTTGACTTGTCTCAGATACCCTATATTAATCTTGTGAAGCATTTAACATCTGCCTGTCCAAAT
  GTATGTCGTATATCACGGTTTCATCACACAACCCCAGACAGTAAAACACACAGTGGTGAAAAATACACCG
  ATCCTTTCAAACTCGGTTGGAGAGACTTGAAAGGTCTGTATGAGGACATTAGAAAGGAACTGCTTATATC
  AACATCAGAACTTAAGGAAATGTCTGAGTACTACTTTGATGGGAAAGGGAAAGCCTTTCGACCAATTATT
  GTGGCGCTAATGGCCCGAGCATGCAATATTCATCATAACAACTCCCGACATGTGCAAGCCAGCCAGCGCG
  CCATAGCCTTAATTGCAGAAATGATCCACACTGCTAGTCTGGTTCACGATGACGTTATTGACGATGCAAG
  TTCTCGAAGAGGAAAACACACAGTTAATAAGATCTGGGGTGAAAAGAAGGCTGTTCTTGCTGGAGATTTA
  ATTCTTTCTGCAGCATCTATAGCTCTGGCACGAATTGGAAATACAACTGTTATATCTATTTTAACCCAAG
  TTATTGAAGATTTGGTGCGTGGTGAATTTCTTCAGCTCGGGTCAAAAGAAAATGAGAATGAAAGATTTGC
  ACACTACCTTGAGAAGACATTCAAGAAGACCGCCAGCCTGATAGCCAACAGTTGTAAAGCAGTCTCTGTT
  CTAGGATGTCCCGACCCAGTGGTGCATGAGATCGCCTATCAGTACGGAAAAAATGTAGGAATAGCTTTTC
  AGCTAATAGATGATGTATTGGACTTCACCTCGTGTTCTGACCAGATGGGCAAACCAACATCAGCTGATCT
  GAAGCTCGGGTTAGCCACTGGTCCTGTCCTGTTTGCCTGTCAGCAGTTCCCAGAAATGAATGCTATGATC
  ATGCGACGGTTCAGTTTGCCTGGAGATGTAGACAGAGCTCGACAGTATGTACTACAGAGTGATGGTGTGC
  AACAAACAACCTACCTCGCCCAGCAGTACTGCCATGAAGCAATAAGAGAGATCAGTAAACTTCGACCATC
  CCCAGAAAGAGATGCCCTCATTCAGCTTTCAGAAATTGTACTCACAAGAGATAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212263 representing NM_014317
  Red=Cloning site Green=Tags(s)
  
  MASRWWRWRRGCSWKPAARSPGPGSPGRAGPLGPSAAAEVRAQVHRRKGLDLSQIPYINLVKHLTSACPN
  VCRISRFHHTTPDSKTHSGEKYTDPFKLGWRDLKGLYEDIRKELLISTSELKEMSEYYFDGKGKAFRPII
  VALMARACNIHHNNSRHVQASQRAIALIAEMIHTASLVHDDVIDDASSRRGKHTVNKIWGEKKAVLAGDL
  ILSAASIALARIGNTTVISILTQVIEDLVRGEFLQLGSKENENERFAHYLEKTFKKTASLIANSCKAVSV
  LGCPDPVVHEIAYQYGKNVGIAFQLIDDVLDFTSCSDQMGKPTSADLKLGLATGPVLFACQQFPEMNAMI
  MRRFSLPGDVDRARQYVLQSDGVQQTTYLAQQYCHEAIREISKLRPSPERDALIQLSEIVLTRDK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014317
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014317.5
• RefSeq Size: 1679 bp
• RefSeq ORF: 1248 bp
• Locus ID: 23590
• UniProt ID: Q5T2R2
• Cytogenetics: 10p12.1
• Protein Pathways: Terpenoid backbone biosynthesis
• MW: 46.1 kDa
• Gene Summary: The protein encoded by this gene is an enzyme that elongates the prenyl side-chain of coenzyme Q, or ubiquinone, one of the key elements in the respiratory chain. The gene product catalyzes the formation of all trans-polyprenyl pyrophosphates from isopentyl diphosphate in the assembly of polyisoprenoid side chains, the first step in coenzyme Q biosynthesis. The protein may be peripherally associated with the inner mitochondrial membrane, though no transit peptide has been definitively identified to date. Defects in this gene are a cause of coenzyme Q10 deficiency. [provided by RefSeq, Jul 2008]