VLDL Receptor (VLDLR) (NM_003383) Human Tagged ORF Clone

CAT#: RC211796

VLDLR (Myc-DDK-tagged)-Human very low density lipoprotein receptor (VLDLR), transcript variant 1

Product Images

Circular map for RC211796

• 

  Western blot validation of overexpression lysate (Cat# LY401153) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211796 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VLDL Receptor
• Synonyms: CAMRQ1; CARMQ1; CHRMQ1; VLDL-R; VLDLRCH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211796 representing NM_003383
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACGTCCGCGCTCTGGGCGCTCTGGCTGCTGCTCGCGCTGTGCTGGGCGCCCCGGGAGAGCGGCG
  CCACCGGAACCGGGAGAAAAGCCAAATGTGAACCCTCCCAATTCCAGTGCACAAATGGTCGCTGTATTAC
  GCTGTTGTGGAAATGTGATGGGGATGAAGACTGTGTTGACGGCAGTGATGAAAAGAACTGTGTAAAGAAG
  ACGTGTGCTGAATCTGACTTCGTGTGCAACAATGGCCAGTGTGTTCCCAGCCGATGGAAGTGTGATGGAG
  ATCCTGACTGCGAAGATGGTTCAGATGAAAGCCCAGAACAGTGCCATATGAGAACATGCCGCATACATGA
  AATCAGCTGTGGCGCCCATTCTACTCAGTGTATCCCAGTGTCCTGGAGATGTGATGGTGAAAATGATTGT
  GACAGTGGAGAAGATGAAGAAAACTGTGGCAATATAACATGTAGTCCCGACGAGTTCACCTGCTCCAGTG
  GCCGCTGCATCTCCAGGAACTTTGTATGCAATGGCCAGGATGACTGCAGCGATGGCAGTGATGAGCTGGA
  CTGTGCCCCGCCAACCTGTGGCGCCCATGAGTTCCAGTGCAGCACCTCCTCCTGCATCCCCATCAGCTGG
  GTATGCGACGATGATGCAGACTGCTCCGACCAATCTGATGAGTCCCTGGAGCAGTGTGGCCGTCAGCCAG
  TCATACACACCAAGTGTCCAGCCAGCGAAATCCAGTGCGGCTCTGGCGAGTGCATCCATAAGAAGTGGCG
  ATGTGATGGGGACCCTGACTGCAAGGATGGCAGTGATGAGGTCAACTGTCCCTCTCGAACTTGCCGACCT
  GACCAATTTGAATGTGAGGATGGCAGCTGCATCCATGGCAGCAGGCAGTGTAATGGTATCCGAGACTGTG
  TCGATGGTTCCGATGAAGTCAACTGCAAAAATGTCAATCAGTGCTTGGGCCCTGGAAAATTCAAGTGCAG
  AAGTGGAGAATGCATAGATATCAGCAAAGTATGTAACCAGGAGCAGGACTGCAGGGACTGGAGTGATGAG
  CCCCTGAAAGAGTGTCATATAAACGAATGCTTGGTAAATAATGGTGGATGTTCTCATATCTGCAAAGACC
  TAGTTATAGGCTACGAGTGTGACTGTGCAGCTGGGTTTGAACTGATAGATAGGAAAACCTGTGGAGATAT
  TGATGAATGCCAAAATCCAGGAATCTGCAGTCAAATTTGTATCAACTTAAAAGGCGGTTACAAGTGTGAA
  TGTAGTCGTGGCTATCAAATGGATCTTGCTACTGGCGTGTGCAAGGCAGTAGGCAAAGAGCCAAGTCTGA
  TCTTCACTAATCGAAGAGACATCAGGAAGATTGGCTTAGAGAGGAAAGAATATATCCAACTAGTTGAACA
  GCTAAGAAACACTGTGGCTCTCGATGCTGACATTGCTGCCCAGAAACTATTCTGGGCCGATCTAAGCCAA
  AAGGCTATCTTCAGTGCCTCAATTGATGACAAGGTTGGTAGACATGTTAAAATGATCGACAATGTCTATA
  ATCCTGCAGCCATTGCTGTTGATTGGGTGTACAAGACCATCTACTGGACTGATGCGGCTTCTAAGACTAT
  TTCAGTAGCTACCCTAGATGGAACCAAGAGGAAGTTCCTGTTTAACTCTGACTTGCGAGAGCCTGCCTCC
  ATAGCTGTGGACCCACTGTCTGGCTTTGTTTACTGGTCAGACTGGGGTGAACCAGCTAAAATAGAAAAAG
  CAGGAATGAATGGATTCGATAGACGTCCACTGGTGACAGCGGATATCCAGTGGCCTAACGGAATTACACT
  TGACCTTATAAAAAGTCGCCTCTATTGGCTTGATTCTAAGTTGCACATGTTATCCAGCGTGGACTTGAAT
  GGCCAAGATCGTAGGATAGTACTAAAGTCTCTGGAGTTCCTAGCTCATCCTCTTGCACTAACAATATTTG
  AGGATCGTGTCTACTGGATAGATGGGGAAAATGAAGCAGTCTATGGTGCCAATAAATTCACTGGATCAGA
  GCTAGCCACTCTAGTCAACAACCTGAATGATGCCCAAGACATCATTGTCTATCATGAACTTGTACAGCCA
  TCAGGTAAAAATTGGTGTGAAGAAGACATGGAGAATGGAGGATGTGAATACCTATGCCTGCCAGCACCAC
  AGATTAATGATCACTCTCCAAAATATACCTGTTCCTGTCCCAGTGGGTACAATGTAGAGGAAAATGGCCG
  AGACTGTCAAAGTACTGCAACTACTGTGACTTACAGTGAGACAAAAGATACGAACACAACAGAAATTTCA
  GCAACTAGTGGACTAGTTCCTGGAGGGATCAATGTGACCACAGCAGTATCAGAGGTCAGTGTTCCCCCAA
  AAGGGACTTCTGCCGCATGGGCCATTCTTCCTCTCTTGCTCTTAGTGATGGCAGCAGTAGGTGGCTACTT
  GATGTGGCGGAATTGGCAACACAAGAACATGAAAAGCATGAACTTTGACAATCCTGTGTACTTGAAAACC
  ACTGAAGAGGACCTCTCCATAGACATTGGTAGACACAGTGCTTCTGTTGGACACACGTACCCAGCAATAT
  CAGTTGTAAGCACAGATGATGATCTAGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211796 representing NM_003383
  Red=Cloning site Green=Tags(s)
  
  MGTSALWALWLLLALCWAPRESGATGTGRKAKCEPSQFQCTNGRCITLLWKCDGDEDCVDGSDEKNCVKK
  TCAESDFVCNNGQCVPSRWKCDGDPDCEDGSDESPEQCHMRTCRIHEISCGAHSTQCIPVSWRCDGENDC
  DSGEDEENCGNITCSPDEFTCSSGRCISRNFVCNGQDDCSDGSDELDCAPPTCGAHEFQCSTSSCIPISW
  VCDDDADCSDQSDESLEQCGRQPVIHTKCPASEIQCGSGECIHKKWRCDGDPDCKDGSDEVNCPSRTCRP
  DQFECEDGSCIHGSRQCNGIRDCVDGSDEVNCKNVNQCLGPGKFKCRSGECIDISKVCNQEQDCRDWSDE
  PLKECHINECLVNNGGCSHICKDLVIGYECDCAAGFELIDRKTCGDIDECQNPGICSQICINLKGGYKCE
  CSRGYQMDLATGVCKAVGKEPSLIFTNRRDIRKIGLERKEYIQLVEQLRNTVALDADIAAQKLFWADLSQ
  KAIFSASIDDKVGRHVKMIDNVYNPAAIAVDWVYKTIYWTDAASKTISVATLDGTKRKFLFNSDLREPAS
  IAVDPLSGFVYWSDWGEPAKIEKAGMNGFDRRPLVTADIQWPNGITLDLIKSRLYWLDSKLHMLSSVDLN
  GQDRRIVLKSLEFLAHPLALTIFEDRVYWIDGENEAVYGANKFTGSELATLVNNLNDAQDIIVYHELVQP
  SGKNWCEEDMENGGCEYLCLPAPQINDHSPKYTCSCPSGYNVEENGRDCQSTATTVTYSETKDTNTTEIS
  ATSGLVPGGINVTTAVSEVSVPPKGTSAAWAILPLLLLVMAAVGGYLMWRNWQHKNMKSMNFDNPVYLKT
  TEEDLSIDIGRHSASVGHTYPAISVVSTDDDLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003383
• ORF Size: 2619 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003383.5
• RefSeq Size: 3646 bp
• RefSeq ORF: 2622 bp
• Locus ID: 7436
• UniProt ID: P98155
• Cytogenetics: 9p24.2
• Domains: ldl_recept_b, EGF_CA, ldl_recept_a, EGF
• Protein Families: Druggable Genome, Transmembrane
• MW: 96.1 kDa
• Gene Summary: The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. This gene encodes a lipoprotein receptor that is a member of the LDLR family and plays important roles in VLDL-triglyceride metabolism and the reelin signaling pathway. Mutations in this gene cause VLDLR-associated cerebellar hypoplasia. Alternative splicing generates multiple transcript variants encoding distinct isoforms for this gene. [provided by RefSeq, Aug 2009]