Cadherin 9 (CDH9) (NM_016279) Human Tagged ORF Clone

CAT#: RC211785

CDH9 (Myc-DDK-tagged)-Human cadherin 9, type 2 (T1-cadherin) (CDH9)

Product Images

Circular map for RC211785

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cadherin 9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211785 representing NM_016279
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGACTTACCATTATATACCATTATTCATCTGGACCTATATGTTCCATACAGTTGACACCATCCTAT
  TACAAGAAAAACCTAACAGTTATTTATCAAGCAAAAAGATAGCGGGTCTGACAAAAGATGACGGTAAAAT
  GCTACGTCGCACCAAGCGTGGCTGGATGTGGAATCAGTTCTTCTTATTGGAAGAGTACACAGGTACTGAC
  ACACAATATGTAGGCAAGCTTCACACTGACCAAGATAAAGGAGATGGAAATTTAAAATACATACTAACAG
  GAGATGGGGCTGGCAGTCTATTTGTTATAGATGAAAATACAGGAGACATTCATGCTGCAAAGAAACTAGA
  CAGAGAAGAAAAATCTCTGTACATTCTTCGTGCCAAGGCTATAGACAGAAAAACTGGGCGGCAGGTGGAA
  CCGGAATCGGAATTTATCATTAAAATACATGATATCAATGACAATGAGCCAAAATTTACAAAAGACTTAT
  ACACTGCCAGTGTTCCTGAAATGTCTGGAGTCGGTACATCTGTTATACAAGTAACTGCAACAGATGCAGA
  TGACGCCAACTATGGAAATAGTGCCAAAGTGGTCTATAGCATATTGCAAGGACAGCCATATTTTTCAGTG
  GACCCAGAATCAGGCATAATAAAAACTGCATTACCAGACATGAGCAGAGAAAATAGAGAGCAGTACCAGG
  TTGTTATACAGGCCAAAGACATGGGTGGCCAGATGGGAGGCCTTTCTGGAACCACCACAGTGAACATCAC
  GCTGACAGATGTCAACAACAACCCTCCTCGATTTCCCCAGAGTACGTATCAATTTAATTCTCCTGAGTCT
  GTACCTCTTGGAACTCATCTTGGAAGGATAAAAGCCAATGACCCTGACGTGGGGGAAAATGCAGAAATGG
  AGTATAGCATTGCTGAAGGAGATGGTGCAGACATGTTCGATGTCATCACTGACAAGGATACACAGGAAGG
  GATTATAACTGTCAAACAGAATTTAGATTTTGAAAATCAAATGCTCTATACTTTAAGAGTGGATGCAAGT
  AACACTCACCCTGATCCACGATTCTTACACCTGGGACCTTTCAAAGATACAGCTGTGGTCAAAATATCTG
  TGGAAGATATAGATGAGCCTCCTGTGTTCACTAAAGTCTCTTACTTGATAGAAGTAGATGAAGATGTAAA
  GGAGGGCAGTATCATTGGACAGGTTACAGCATACGATCCAGATGCCAGGAACAATTTAATAAAGTACTCT
  GTTGATCGGCATACTGATATGGACCGTATTTTTGGTATTCACTCAGAAAATGGTTCTATTTTCACTTTGA
  AAGCCCTTGACCGGGAATCATCTCCTTGGCATAACATCACTGTTACAGCCACAGAAATAAATAACCCAAA
  ACAAAGTAGCCACATCCCTGTCTTCATCAGAATTCTAGATATAAATGACCATGCTCCGGAATTTGCCATG
  TATTATGAAACATTTGTTTGTGAAAATGCAAAACCTGGGCAGTTGATTCAGACTGTCAGTGTCATGGATA
  AGGATGACCCTCCCCGAGGTCACAAATTCTTTTTTGAACCAGTGCCAGAATTTACTCTCAATCCGAATTT
  CACCATTGTAGATAATAAAGATAATACAGCAGGAATCATGACTCGGAAAGATGGCTACAGTCGCAACAAA
  ATGAGCACCTACTTATTGCCGATTTTAATCTTTGACAACGATTATCCAATTCAAAGCAGCACTGGTACAC
  TCACTATCCGTGTGTGTGCCTGCGATAATCAAGGAAACATGCAATCCTGCACCGCAGAAGCCCTGATCCT
  TTCAGCCGGCCTGAGCACGGGAGCTCTCGTTGCGATTCTACTCTGTGTCCTCATACTGCTTATTTTAGTC
  GTGTTGTTTGCTGCATTGAAGAGGCAAAGAAAAAAGGAACCTCTGATAATTTCAAAAGACGATGTCCGGG
  ACAACATTGTGACCTACAACGATGAAGGCGGCGGGGAAGAAGATACCCAAGCTTTTGACATTGGCACATT
  AAGGAATCCAGAGGCAAGAGAAGACAGTAAACTTAGACGGGATGTAATGCCTGAAACTATTTTTCAGATA
  AGGAGGACTGTGCCTCTGTGGGAAAATATTGATGTACAAGATTTTATCCATCGAAGATTAAAAGAAAACG
  ACGCAGACCCAAGTGCACCTCCATATGATTCGCTGGCAACGTATGCCTATGAAGGGAATGATTCCATAGC
  AGATTCGCTCAGTTCTTTGGAATCTCTCACAGCTGATTGTAACCAAGATTATGATTACCTCAGTGACTGG
  GGGCCTCGTTTCAAAAAACTTGCCGATATGTATGGGGGTGATGATAGTGACCGAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211785 representing NM_016279
  Red=Cloning site Green=Tags(s)
  
  MRTYHYIPLFIWTYMFHTVDTILLQEKPNSYLSSKKIAGLTKDDGKMLRRTKRGWMWNQFFLLEEYTGTD
  TQYVGKLHTDQDKGDGNLKYILTGDGAGSLFVIDENTGDIHAAKKLDREEKSLYILRAKAIDRKTGRQVE
  PESEFIIKIHDINDNEPKFTKDLYTASVPEMSGVGTSVIQVTATDADDANYGNSAKVVYSILQGQPYFSV
  DPESGIIKTALPDMSRENREQYQVVIQAKDMGGQMGGLSGTTTVNITLTDVNNNPPRFPQSTYQFNSPES
  VPLGTHLGRIKANDPDVGENAEMEYSIAEGDGADMFDVITDKDTQEGIITVKQNLDFENQMLYTLRVDAS
  NTHPDPRFLHLGPFKDTAVVKISVEDIDEPPVFTKVSYLIEVDEDVKEGSIIGQVTAYDPDARNNLIKYS
  VDRHTDMDRIFGIHSENGSIFTLKALDRESSPWHNITVTATEINNPKQSSHIPVFIRILDINDHAPEFAM
  YYETFVCENAKPGQLIQTVSVMDKDDPPRGHKFFFEPVPEFTLNPNFTIVDNKDNTAGIMTRKDGYSRNK
  MSTYLLPILIFDNDYPIQSSTGTLTIRVCACDNQGNMQSCTAEALILSAGLSTGALVAILLCVLILLILV
  VLFAALKRQRKKEPLIISKDDVRDNIVTYNDEGGGEEDTQAFDIGTLRNPEAREDSKLRRDVMPETIFQI
  RRTVPLWENIDVQDFIHRRLKENDADPSAPPYDSLATYAYEGNDSIADSLSSLESLTADCNQDYDYLSDW
  GPRFKKLADMYGGDDSDRD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016279
• ORF Size: 2367 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016279.4
• RefSeq Size: 3075 bp
• RefSeq ORF: 2370 bp
• Locus ID: 1007
• UniProt ID: Q9ULB4
• Cytogenetics: 5p14.1
• Domains: Cadherin_C_term, CA
• Protein Families: Transmembrane
• MW: 89.1 kDa
• Gene Summary: This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. [provided by RefSeq, Jul 2008]