SFRS14 (SUGP2) (NM_001017392) Human Tagged ORF Clone

CAT#: RC211778

SUGP2 (Myc-DDK-tagged)-Human SURP and G patch domain containing 2 (SUGP2), transcript variant 1

Product Images

Circular map for RC211778

• 

  Western blot validation of overexpression lysate (Cat# LY422773) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211778 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SFRS14
• Synonyms: SFRS14; SRFS14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211778 representing NM_001017392
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCAGACGAATTACACAGGAGACTTTTGATGCTGTATTACAAGAAAAAGCCAAACGATATCACA
  TGGATGCCAGTGGTGAGGCTGTAAGCGAAACTCTTCAGTTTAAAGCTCAAGATCTCTTAAGGGCAGTCCC
  AAGATCCAGAGCAGAGATGTATGATGACGTCCACAGCGATGGCAGATACTCCCTCAGTGGATCTGTAGCT
  CACTCTAGAGATGCCGGAAGAGAAGGCCTGAGAAGTGACGTATTTCCAGGGCCTTCCTTCAGATCAAGCA
  ACCCTTCCATCAGTGATGACAGCTACTTTCGCAAAGAATGTGGCCGGGATCTGGAATTTTCTCACTCTGA
  TTCTCGGGACCAGGTCATTGGCCACCGGAAATTGGGGCATTTCCGTTCTCAGGACTGGAAATTTGCGCTC
  CGTGGTTCTTGGGAACAAGACTTTGGCCATCCAGTTTCTCAAGAGTCCTCTTGGTCACAGGAGTATAGTT
  TTGGTCCCTCTGCAGTTTTGGGGGACTTTGGATCTTCCAGGCTGATTGAGAAAGAGTGTTTGGAGAAGGA
  GAGTCGGGATTATGACGTGGACCATCCTGGGGAGGCTGACTCTGTGCTTAGGGGCGGCAGTCAAGTCCAG
  GCCAGAGGTCGAGCTCTAAACATCGTTGACCAGGAAGGTTCCCTCCTAGGAAAGGGGGAGACTCAGGGCC
  TGCTCACAGCTAAGGGGGGTGTTGGGAAACTTGTCACATTGAGAAATGTGAGCACAAAAAAAATACCCAC
  CGTGAATCGTATTACTCCCAAAACTCAGGGCACTAACCAAATCCAGAAAAACACTCCAAGTCCTGATGTG
  ACCCTGGGGACAAACCCAGGGACAGAAGATATCCAGTTCCCCATTCAGAAGATCCCTCTGGGGCTGGATC
  TGAAGAATCTTCGGCTCCCCAGAAGAAAGATGAGCTTTGACATCATAGATAAGTCTGATGTTTTTTCAAG
  ATTTGGGATAGAAATAATCAAATGGGCAGGATTCCACACCATAAAAGATGATATTAAATTTTCCCAACTT
  TTCCAGACTCTCTTTGAACTTGAAACAGAAACCTGTGCTAAAATGCTTGCCTCATTCAAATGTTCCTTAA
  AACCAGAGCACAGAGATTTTTGCTTTTTTACTATCAAATTTTTAAAGCACTCTGCTTTGAAAACACCCAG
  AGTTGATAATGAGTTTTTAAACATGCTTTTAGACAAAGGTGCTGTGAAGACCAAAAATTGCTTTTTTGAA
  ATCATAAAGCCTTTTGACAAGTACATAATGAGACTTCAAGACCGGCTTCTGAAGAGTGTCACACCTTTGC
  TTATGGCCTGCAATGCCTACGAGCTAAGTGTCAAGATGAAGACCCTCAGTAACCCCCTGGACTTGGCTCT
  TGCCCTAGAAACCACCAACTCTCTCTGCCGGAAGTCTTTGGCCCTTTTGGGACAGACATTTTCCTTGGCC
  TCTTCTTTCCGGCAGGAGAAAATCTTAGAAGCTGTCGGCCTGCAAGATATAGCTCCCTCACCTGCTGCGT
  TTCCAAACTTCGAAGACTCCACTTTGTTTGGGCGAGAGTACATAGACCACCTGAAGGCCTGGCTAGTCAG
  CAGCGGATGTCCCCTCCAGGTTAAGAAAGCCGAACCAGAGCCGATGCGAGAGGAGGAGAAAATGATTCCT
  CCTACGAAACCTGAAATTCAGGCCAAGGCTCCAAGTAGTCTGAGTGATGCTGTCCCCCAGCGAGCAGATC
  ACAGGGTAGTGGGCACCATCGACCAGCTTGTGAAACGTGTCATCGAAGGCAGCCTGTCTCCCAAAGAGAG
  AACTCTTCTCAAAGAGGACCCTGCTTACTGGTTTTTGTCTGATGAAAATAGTCTGGAGTATAAATATTAC
  AAGCTGAAGTTGGCAGAAATGCAGCGGATGAGCGAGAACTTGCGAGGAGCCGACCAGAAGCCGACCTCAG
  CAGACTGTGCAGTGAGGGCCATGCTGTACTCCCGGGCTGTCCGCAACCTCAAGAAGAAACTCCTTCCGTG
  GCAGCGGCGGGGGCTCCTCCGTGCTCAAGGGCTCCGGGGCTGGAAGGCGAGGAGAGCGACCACCGGGACC
  CAGACCCTCCTATCCTCAGGCACCAGGCTGAAACACCACGGCCGGCAGGCTCCAGGCCTCTCACAGGCAA
  AACCATCCCTGCCAGACAGAAATGATGCTGCCAAGGACTGCCCGCCAGACCCAGTTGGACCTTCTCCTCA
  GGACCCCAGCTTAGAAGCCTCAGGCCCATCCCCCAAGCCAGCAGGAGTGGACATCTCTGAAGCACCTCAG
  ACCTCTTCTCCCTGCCCATCTGCTGACATTGACATGAAGACAATGGAGACTGCAGAGAAACTGGCTAGAT
  TTGTTGCTCAGGTGGGACCAGAGATCGAACAATTCAGCATAGAAAACAGCACCGATAACCCTGACCTGTG
  GTTTCTACATGACCAAAATAGTTCTGCTTTCAAATTCTATCGAAAGAAAGTGTTTGAACTATGTCCATCA
  ATTTGTTTCACGTCATCTCCGCACAACCTTCACACTGGTGGTGGTGACACCACGGGTTCTCAGGAGAGCC
  CCGTGGACCTCATGGAAGGGGAAGCAGAGTTTGAAGACGAGCCCCCTCCGCGGGAGGCTGAGCTGGAGAG
  CCCAGAGGTGATGCCTGAGGAGGAGGACGAGGACGATGAGGATGGGGGAGAGGAGGCCCCCGCTCCTGGA
  GGGGCGGGCAAGTCTGAGGGCAGCACCCCTGCCGACGGCCTTCCCGGCGAGGCTGCCGAGGACGACCTGG
  CTGGAGCACCTGCCTTGTCACAGGCCTCCTCAGGTACCTGCTTCCCTCGGAAGAGGATCAGCAGCAAGTC
  ATTGAAGGTTGGCATGATTCCAGCTCCCAAGAGAGTGTGTCTCATCCAGGAGCCAAAAGTCCATGAACCA
  GTTCGAATTGCCTATGACAGGCCTCGGGGTCGTCCCATGTCCAAAAAGAAGAAACCCAAGGACTTGGACT
  TCGCCCAGCAGAAGCTGACCGATAAGAACCTGGGCTTCCAGATGCTGCAGAAGATGGGCTGGAAGGAGGG
  CCATGGCCTGGGCTCCCTCGGAAAGGGCATCAGGGAGCCGGTCAGCGTGGGAACCCCCTCGGAAGGGGAA
  GGGTTGGGTGCTGACGGGCAGGAGCACAAAGAAGACACATTCGATGTGTTCCGACAGAGGATGATGCAGA
  TGTACAGACACAAGCGGGCCAACAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211778 representing NM_001017392
  Red=Cloning site Green=Tags(s)
  
  MAARRITQETFDAVLQEKAKRYHMDASGEAVSETLQFKAQDLLRAVPRSRAEMYDDVHSDGRYSLSGSVA
  HSRDAGREGLRSDVFPGPSFRSSNPSISDDSYFRKECGRDLEFSHSDSRDQVIGHRKLGHFRSQDWKFAL
  RGSWEQDFGHPVSQESSWSQEYSFGPSAVLGDFGSSRLIEKECLEKESRDYDVDHPGEADSVLRGGSQVQ
  ARGRALNIVDQEGSLLGKGETQGLLTAKGGVGKLVTLRNVSTKKIPTVNRITPKTQGTNQIQKNTPSPDV
  TLGTNPGTEDIQFPIQKIPLGLDLKNLRLPRRKMSFDIIDKSDVFSRFGIEIIKWAGFHTIKDDIKFSQL
  FQTLFELETETCAKMLASFKCSLKPEHRDFCFFTIKFLKHSALKTPRVDNEFLNMLLDKGAVKTKNCFFE
  IIKPFDKYIMRLQDRLLKSVTPLLMACNAYELSVKMKTLSNPLDLALALETTNSLCRKSLALLGQTFSLA
  SSFRQEKILEAVGLQDIAPSPAAFPNFEDSTLFGREYIDHLKAWLVSSGCPLQVKKAEPEPMREEEKMIP
  PTKPEIQAKAPSSLSDAVPQRADHRVVGTIDQLVKRVIEGSLSPKERTLLKEDPAYWFLSDENSLEYKYY
  KLKLAEMQRMSENLRGADQKPTSADCAVRAMLYSRAVRNLKKKLLPWQRRGLLRAQGLRGWKARRATTGT
  QTLLSSGTRLKHHGRQAPGLSQAKPSLPDRNDAAKDCPPDPVGPSPQDPSLEASGPSPKPAGVDISEAPQ
  TSSPCPSADIDMKTMETAEKLARFVAQVGPEIEQFSIENSTDNPDLWFLHDQNSSAFKFYRKKVFELCPS
  ICFTSSPHNLHTGGGDTTGSQESPVDLMEGEAEFEDEPPPREAELESPEVMPEEEDEDDEDGGEEAPAPG
  GAGKSEGSTPADGLPGEAAEDDLAGAPALSQASSGTCFPRKRISSKSLKVGMIPAPKRVCLIQEPKVHEP
  VRIAYDRPRGRPMSKKKKPKDLDFAQQKLTDKNLGFQMLQKMGWKEGHGLGSLGKGIREPVSVGTPSEGE
  GLGADGQEHKEDTFDVFRQRMMQMYRHKRANK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001017392
• ORF Size: 3246 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017392.5
• RefSeq Size: 4717 bp
• RefSeq ORF: 3249 bp
• Locus ID: 10147
• UniProt ID: Q8IX01
• Cytogenetics: 19p13.11
• MW: 120 kDa
• Gene Summary: This gene encodes a member of the arginine/serine-rich family of splicing factors. The encoded protein functions in mRNA processing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009]