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CAIN (CABIN1) (NM_012295) Human Tagged ORF Clone
CAT#: RC211750
- TrueORF®
CABIN1 (Myc-DDK-tagged)-Human calcineurin binding protein 1 (CABIN1), transcript variant 2
ORF Plasmid: tGFP
"NM_012295" in other vectors (2)
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Frequently bought together (5)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | CAIN |
| Synonyms | CAIN; KB-318B8.7; PPP3IN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211750 representing NM_012295
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCGAATTGCAGCCTTAAATGCCAGCTCCACCATTGAGGATGATCATGAAGGAAGCTTTAAAAGTC ACAAAACCCAGACAAAGGAGGCTCAGGAAGCAGAGGCTTTTGCATTGTACCACAAGGCCCTTGATCTGCA GAAACATGACCGGTTTGAGGAGTCTGCCAAAGCCTACCATGAGCTCTTGGAGGCGAGCCTGCTGCGGGAG GCAGTTTCATCCGGTGATGAGAAAGAGGGGTTGAAACACCCTGGGCTGATACTGAAATATTCCACTTATA AGAACTTGGCCCAGCTGGCAGCCCAGCGGGAGGATCTGGAGACAGCCATGGAGTTCTACTTAGAGGCAGT GATGCTGGACTCCACAGATGTCAACCTCTGGTATAAGATTGGACATGTGGCCCTGAGGCTCATCCGGATC CCCCTGGCTCGCCATGCTTTTGAGGAAGGGCTGCGGTGCAATCCTGACCACTGGCCCTGTTTGGATAACC TAATCACTGTCCTGTACACCCTCAGTGATTACACAACATGTCTGTACTTCATCTGCAAAGCTTTGGAGAA GGATTGCCGGTACAGCAAAGGGCTGGTCCTCAAGGAGAAGATTTTTGAGGAGCAGCCTTGTCTCCGGAAG GACTCTCTCAGAATGTTCCTCAAATGTGACATGTCGATTCACGATGTTTCGGTGAGTGCAGCTGAGACAC AGGCGATTGTAGATGAGGCCTTGGGGCTGCGAAAAAAGAGGCAAGCGCTGATTGTGCGGGAGAAGGAGCC GGACCTGAAACTTGTGCAGCCCATTCCTTTCTTCACCTGGAAGTGCCTCGGAGAGAGCTTGCTGGCCATG TACAATCATCTCACCACCTGTGAGCCCCCACGTCCCAGCCTTGGCAAAAGGATTGATTTGTCGGACTACC AGGACCCCAGCCAGCCTCTTGAGTCCTCCATGGTGGTGACGCCAGTTAACGTGATCCAGCCAAGCACTGT CAGCACCAACCCAGCTGTGGCTGTCGCCGAGCCTGTGGTCTCCTACACCTCTGTGGCTACAACCAGCTTC CCACTGCACAGTCCTGGTCTGTTGGAGACAGGCGCTCCTGTGGGTGATATTTCTGGGGGAGATAAATCCA AGAAAGGGGTAAAACGGAAGAAGATTTCAGAAGAGAGTGGAGAAACAGCAAAGCGGCGGTCTGCCCGTGT CCGAAACACCAAGTGCAAAAAAGAAGAGAAAGTAGACTTCCAGGAGCTTCTGATGAAGTTCTTGCCGTCC AGGTTAAGAAAGCTGGACCCTGAGGAGGAAGATGATTCCTTTAATAACTATGAAGTCCAGTCAGAAGCCA AACTGGAAAGCTTCCCAAGCATTGGGCCTCAAAGGCTGTCATTTGACTCAGCCACATTCATGGAATCTGA AAAGCAGGACGTGCATGAGTTCCTGCTGGAGAACCTAACCAACGGGGGCATCCTGGAGCTGATGATGCGC TACCTGAAAGCCATGGGCCACAAGTTCTTGGTAAGGTGGCCTCCAGGCTTGGCGGAGGTCGTGCTCAGCG TCTACCACAGCTGGAGGAGGCACAGCACCAGCCTGCCCAACCCGCTGCTGAGGGACTGCAGCAACAAGCA CATCAAGGACATGATGCTGATGTCTCTCTCCTGCATGGAACTCCAGCTGGACCAGTGGCTGCTGACCAAA GGCAGAAGCTCTGCAGTGTCTCCTCGGAACTGTCCTGCTGGTATGGTGAATGGCAGATTTGGACCTGACT TCCCAGGGACCCACTGCCTGGGTGACCTCCTACAGCTGTCATTTGCCTCGTCCCAGCGCGACCTGTTCGA GGATGGTTGGCTGGAGTTTGTGGTCCGTGTTTACTGGCTGAAGGCTCGCTTCCTGGCGCTGCAGGGAGAC ATGGAGCAGGCCCTGGAGAACTATGACATCTGCACAGAAATGCTCCAGAGTTCCACCGCCATCCAGGTGG AGGCAGGGGCTGAACGAAGAGACATTGTCATCCGGCTGCCCAACCTCCATAATGACTCTGTGGTTTCCCT GGAGGAGATTGATAAGAACCTGAAGTCGCTGGAGCGGTGCCAGTCCCTGGAGGAGATTCAGCGGCTGTAT GAAGCAGGCGACTACAAGGCTGTTGTGCATCTGCTCCGCCCCACTTTGTGCACCAGTGGGTTTGACCGGG CCAAACACCTGGAGTTTATGACTTCCATTCCTGAGAGGCCAGCCCAGCTGCTTCTTCTGCAGGACTCCTT GCTCCGGCTGAAGGACTATCGGCAGTGTTTTGAGTGTTCCGATGTGGCTCTGAACGAGGCTGTCCAGCAG ATGGTGAACTCAGGTGAGGCTGCCGCCAAGGAGGAGTGGGTGGCCACAGTGACCCAACTGCTGATGGGCA TCGAGCAGGCCCTCTCTGCGGACAGCAGTGGTAGCATCCTGAAGGTATCATCCTCCACCACTGGCCTTGT GCGGCTCACCAACAACCTCATCCAGGTCATTGACTGCAGCATGGCTGTGCAGGAGGAGGCCAAGGAGCCC CACGTCTCTTCAGTGCTACCCTGGATCATTCTACACCGGATCATCTGGCAGGAGGAAGACACCTTCCATT CTCTGTGCCACCAGCAGCAGCTCCAAAACCCAGCGGAGGAAGGGATGTCAGAGACGCCCATGCTCCCATC CTCCCTCATGCTGCTGAACACAGCCCACGAGTATTTGGGCAGAAGGTCCTGGTGCTGCAATTCAGATGGG GCTCTGCTGCGATTCTATGTGCGAGTACTCCAGAAGGAACTGGCTGCATCCACCTCTGAAGACACGCACC CTTACAAGGAGGAGCTGGAGACAGCCTTGGAGCAGTGCTTCTACTGCCTGTACAGCTTCCCCAGCAAGAA GAGTAAGGCCAGGTACCTGGAGGAACACTCGGCCCAGCAGGTGGATCTTATATGGGAGGATGCACTGTTC ATGTTTGAGTATTTTAAGCCCAAGACCCTTCCTGAATTTGACAGCTATAAGACCAGCACCGTGTCTGCTG ACTTGGCCAACCTACTGAAGAGAATTGCCACCATTGTGCCTCGCACAGAGAGGCCAGCCCTTAGCCTGGA CAAAGTCTCTGCCTACATTGAGGGAACTTCAACTGAGGTACCCTGCCTCCCAGAGGGGGCTGACCCCTCC CCTCCAGTGGTGAACGAGCTTTACTACCTCCTGGCTGATTATCATTTCAAAAACAAGGAGCAGTCCAAGG CCATCAAGTTCTACATGCATGACATCTGCATCTGCCCCAATAGGTTTGATTCCTGGGCAGGCATGGCTCT GGCCCGGGCCAGCCGCATTCAGGACAAGCTGAACTCCAATGAGCTGAAGAGTGATGGGCCCATTTGGAAG CATGCCACGCCCGTCTTGAACTGCTTCCGTCGGGCCCTGGAGATTGACAGCTCCAACTTGTCCCTATGGA TTGAGTATGGCACCATGTCCTATGCCTTGCACTCATTCGCCTCACGTCAATTGAAGCAGTGGAGAGGCGA GCTGCCCCCTGAGCTCGTGCAGCAGATGGAGGGCCGGCGCGACAGCATGCTAGAGACAGCCAAGCACTGT TTCACATCAGCAGCCCGCTGCGAGGGTGATGGTGACGAGGAGGAGTGGCTCATCCACTACATGCTGGGCA AGGTGGCTGAGAAGCAGCAGCAGCCACCCACCGTTTACTTGCTGCACTACAGGCAGGCTGGCCACTACCT GCACGAGGAGGCTGCCCGCTACCCCAAGAAGATCCACTACCACAACCCACCTGAGCTGGCCATGGAGGCC CTGGAGGTGTACTTTCGGCTCCATGCTTCCATCCTGAAGCTCCTGGGGAAGCCCGATTCTGGGGTTGGTG CAGAGGTCCTGGTCAACTTTATGAAGGAGGCTGCAGAAGGACCCTTTGCCAGGGGCGAGGAGAAGAACAC ACCCAAAGCTTCAGAAAAGGAGAAGGCCTGCCTGGTGGACGAGGACTCCCACTCTTCAGCTGGGACACTG CCGGGCCCCGGAGCCTCCCTCCCCTCCTCCTCTGGCCCAGGTCTGACATCCCCACCTTACACAGCCACTC CGATTGACCACGATTACGTCAAATGTAAAAAACCCCACCAGCAGGCAACGCCGGACGACCGAAGCCAGGA CAGCACAGCCGTAGCACTCTCAGACTCTAGCTCAACGCAGGACTTCTTTAATGAGCCCACCAGCTTACTG GAAGGCTCCAGGAAATCCTACACAGAGAAGAGGCTGCCCATTCTCAGTTCCCAAGCAGGAGCGACGGGTA AAGATCTTCAGGGGGCCACAGAAGAAAGAGGAAAAAACGAGGAGTCATTGGAGAGTACAGAAGGCTTCCG GGCTGCAGAGCAAGGTGTCCAGAAGCCTGCTGCAGAAACCCCAGCCTCTGCTTGCATCCCTGGCAAGCCC TCAGCATCCACACCCACCCTGTGGGATGGGAAGAAGAGAGGGGACCTCCCAGGGGAGCCAGTGGCCTTCC CCCAGGGGCTGCCGGCTGGTGCTGAGGAGCAGCGGCAGTTTCTCACAGAGCAGTGCATCGCCTCCTTCCG CCTGTGCCTGAGCCGCTTCCCCCAGCACTATAAGAGTCTCTACCGTCTGGCCTTCCTCTACACCTACAGC AAGACCCACCGGAACCTCCAGTGGGCCCGCGACGTGTTGCTAGGCAGCAGTATCCCGTGGCAACAACTGC AGCACATGCCGGCACAGGGGCTCTTCTGCGAGAGGAACAAGACCAATTTCTTCAACGGCATCTGGCGGAT CCCCGTGGACGAGATTGACCGGCCGGGCAGCTTTGCCTGGCACATGAACCGCTCCATCGTGCTGCTGCTC AAGGTGCTGGCCCAGCTGCGGGACCACAGCACCCTGCTGAAGGTGTCCTCCATGCTTCAGCGGACCCCAG ACCAGGGCAAGAAGTATCTGCGAGATGCTGACCGCCAGGTCCTGGCGCAGCGGGCCTTCATCCTCACTGT GAAGGTGCTCGAAGACACGCTGAGCGAGCTCGCAGAGGGGTCAGAACGCCCAGGGCCCAAGGTCTGTGGC CTCCCCGGAGCCAGGATGACCACCGATGTCTCACACAAGGCCAGTCCTGAGGATGGCCAGGAGGGCCTCC CCCAGCCGAAGAAGCCCCCTCTGGCTGATGGCTCAGGGCCAGGGCCCGAGCCAGGAGGCAAAGTGGGCCT CCTCAACCACCGGCCTGTGGCCATGGATGCAGGAGACAGTGCAGACCAAAGCGGGGAGCGGAAGGATAAA GAGAGCCCACGGGCAGGGCCCACTGAGCCCATGGACACGAGTGAGGCCACTGTTTGCCACTCAGACTTGG AGCGGACACCACCCCTGCTGCCAGGTCGCCCCGCAAGGGACCGGGGCCCCGAGAGCCGGCCCACTGAGCT GTCCCTGGAGGAGCTGAGCATCAGTGCCCGGCAGCAGCCCACCCCGCTCACCCCAGCCCAGCCAGCCCCC GCCCCCGCCCCCGCCACCACCACAGGGACCAGGGCAGGGGGCCACCCGGAGGAGCCGCTCTCCCGGCTCA GCCGCAAGAGGAAGCTCCTGGAGGACACAGAGTCAGGCAAGACACTTCTGTTGGATGCCTACCGTGTGTG GCAGCAGGGCCAGAAGGGTGTGGCCTATGACCTGGGCCGTGTGGAGAGGATCATGTCGGAGACCTACATG CTCATCAAGCAGGTGGATGAGGAGGCTGCGCTGGAGCAGGCTGTGAAGTTCTGCCAGGTCCATCTTGGGG CTGCCGCCCAGAGACAGGCCTCGGGGGACACCCCCACCACTCCAAAGCACCCCAAAGACAGCCGAGAGAA CTTCTTTCCTGTGACAGTGGTGCCCACAGCCCCTGACCCTGTGCCAGCTGACTCTGTCCAGCGGCCCAGT GATGCTCACACCAAGCCTCGCCCTGCACTAGCTGCCGCCACAACTATTATCACCTGCCCTCCGTCAGCAT CAGCTTCCACCCTGGACCAGTCCAAGGACCCTGGGCCTCCCCGGCCACACAGGCCTGAAGCTACCCCCAG CATGGCCTCTCTGGGCCCAGAGGGAGAAGAGCTGGCGAGAGTGGCAGAGGGCACCAGCTTCCCGCCTCAG GAGCCACGGCACAGTCCGCAGGTGAAGATGGCCCCCACAAGTTCCCCGGCAGAGCCACACTGCTGGCCGG CAGAGGCTGCCCTGGGCACAGGCGCTGAGCCCACCTGCAGCCAGGAGGGGAAACTGAGGCCTGAGCCGAG AAGGGATGGGGAGGCTCAGGAGGCTGCGAGTGAGACTCAGCCCCTGAGCTCTCCCCCAACAGCTGCCAGC TCCAAGGCCCCCAGCAGTGGGAGTGCCCAGCCACCAGAGGGTCACCCAGGCAAGCCTGAGCCCAGCCGGG CTAAGTCCCGCCCCCTGCCCAACATGCCAAAGCTGGTCATCCCCTCCGCCGCCACCAAGTTCCCCCCTGA GATCACCGTCACGCCACCCACCCCAACCCTGCTCTCCCCCAAAGGCAGCATCTCGGAGGAGACCAAGCAG AAGCTGAAGTCAGCCATCCTTTCTGCCCAGTCTGCTGCCAACGTGAGGAAGGAGAGCCTATGCCAGCCAG CCCTGGAGGTCCTGGAGACATCCAGCCAGGAGTCCTCGCTGGAGAGCGAGACAGACGAGGACGACGACTA CATGGACATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211750 representing NM_012295
Red=Cloning site Green=Tags(s) MIRIAALNASSTIEDDHEGSFKSHKTQTKEAQEAEAFALYHKALDLQKHDRFEESAKAYHELLEASLLRE AVSSGDEKEGLKHPGLILKYSTYKNLAQLAAQREDLETAMEFYLEAVMLDSTDVNLWYKIGHVALRLIRI PLARHAFEEGLRCNPDHWPCLDNLITVLYTLSDYTTCLYFICKALEKDCRYSKGLVLKEKIFEEQPCLRK DSLRMFLKCDMSIHDVSVSAAETQAIVDEALGLRKKRQALIVREKEPDLKLVQPIPFFTWKCLGESLLAM YNHLTTCEPPRPSLGKRIDLSDYQDPSQPLESSMVVTPVNVIQPSTVSTNPAVAVAEPVVSYTSVATTSF PLHSPGLLETGAPVGDISGGDKSKKGVKRKKISEESGETAKRRSARVRNTKCKKEEKVDFQELLMKFLPS RLRKLDPEEEDDSFNNYEVQSEAKLESFPSIGPQRLSFDSATFMESEKQDVHEFLLENLTNGGILELMMR YLKAMGHKFLVRWPPGLAEVVLSVYHSWRRHSTSLPNPLLRDCSNKHIKDMMLMSLSCMELQLDQWLLTK GRSSAVSPRNCPAGMVNGRFGPDFPGTHCLGDLLQLSFASSQRDLFEDGWLEFVVRVYWLKARFLALQGD MEQALENYDICTEMLQSSTAIQVEAGAERRDIVIRLPNLHNDSVVSLEEIDKNLKSLERCQSLEEIQRLY EAGDYKAVVHLLRPTLCTSGFDRAKHLEFMTSIPERPAQLLLLQDSLLRLKDYRQCFECSDVALNEAVQQ MVNSGEAAAKEEWVATVTQLLMGIEQALSADSSGSILKVSSSTTGLVRLTNNLIQVIDCSMAVQEEAKEP HVSSVLPWIILHRIIWQEEDTFHSLCHQQQLQNPAEEGMSETPMLPSSLMLLNTAHEYLGRRSWCCNSDG ALLRFYVRVLQKELAASTSEDTHPYKEELETALEQCFYCLYSFPSKKSKARYLEEHSAQQVDLIWEDALF MFEYFKPKTLPEFDSYKTSTVSADLANLLKRIATIVPRTERPALSLDKVSAYIEGTSTEVPCLPEGADPS PPVVNELYYLLADYHFKNKEQSKAIKFYMHDICICPNRFDSWAGMALARASRIQDKLNSNELKSDGPIWK HATPVLNCFRRALEIDSSNLSLWIEYGTMSYALHSFASRQLKQWRGELPPELVQQMEGRRDSMLETAKHC FTSAARCEGDGDEEEWLIHYMLGKVAEKQQQPPTVYLLHYRQAGHYLHEEAARYPKKIHYHNPPELAMEA LEVYFRLHASILKLLGKPDSGVGAEVLVNFMKEAAEGPFARGEEKNTPKASEKEKACLVDEDSHSSAGTL PGPGASLPSSSGPGLTSPPYTATPIDHDYVKCKKPHQQATPDDRSQDSTAVALSDSSSTQDFFNEPTSLL EGSRKSYTEKRLPILSSQAGATGKDLQGATEERGKNEESLESTEGFRAAEQGVQKPAAETPASACIPGKP SASTPTLWDGKKRGDLPGEPVAFPQGLPAGAEEQRQFLTEQCIASFRLCLSRFPQHYKSLYRLAFLYTYS KTHRNLQWARDVLLGSSIPWQQLQHMPAQGLFCERNKTNFFNGIWRIPVDEIDRPGSFAWHMNRSIVLLL KVLAQLRDHSTLLKVSSMLQRTPDQGKKYLRDADRQVLAQRAFILTVKVLEDTLSELAEGSERPGPKVCG LPGARMTTDVSHKASPEDGQEGLPQPKKPPLADGSGPGPEPGGKVGLLNHRPVAMDAGDSADQSGERKDK ESPRAGPTEPMDTSEATVCHSDLERTPPLLPGRPARDRGPESRPTELSLEELSISARQQPTPLTPAQPAP APAPATTTGTRAGGHPEEPLSRLSRKRKLLEDTESGKTLLLDAYRVWQQGQKGVAYDLGRVERIMSETYM LIKQVDEEAALEQAVKFCQVHLGAAAQRQASGDTPTTPKHPKDSRENFFPVTVVPTAPDPVPADSVQRPS DAHTKPRPALAAATTIITCPPSASASTLDQSKDPGPPRPHRPEATPSMASLGPEGEELARVAEGTSFPPQ EPRHSPQVKMAPTSSPAEPHCWPAEAALGTGAEPTCSQEGKLRPEPRRDGEAQEAASETQPLSSPPTAAS SKAPSSGSAQPPEGHPGKPEPSRAKSRPLPNMPKLVIPSAATKFPPEITVTPPTPTLLSPKGSISEETKQ KLKSAILSAQSAANVRKESLCQPALEVLETSSQESSLESETDEDDDYMDI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012295 |
| ORF Size | 6660 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012295.2, NP_036427.1 |
| RefSeq Size | 7222 bp |
| RefSeq ORF | 6663 bp |
| Locus ID | 23523 |
| UniProt ID | Q9Y6J0 |
| Cytogenetics | 22q11.23 |
| Domains | TPR |
| Protein Families | Druggable Genome |
| MW | 246.2 kDa |
| Gene Summary | Calcineurin plays an important role in the T-cell receptor-mediated signal transduction pathway. The protein encoded by this gene binds specifically to the activated form of calcineurin and inhibits calcineurin-mediated signal transduction. The encoded protein is found in the nucleus and contains a leucine zipper domain as well as several PEST motifs, sequences which confer targeted degradation to those proteins which contain them. Alternative splicing results in multiple transcript variants encoding two different isoforms. [provided by RefSeq, Jan 2011] |
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