Prominin 2 (PROM2) (NM_144707) Human Tagged ORF Clone

CAT#: RC211605

PROM2 (Myc-DDK-tagged)-Human prominin 2 (PROM2), transcript variant 3

Product Images

Circular map for RC211605

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PROM2 (Cat# RC211605, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROM2(Cat# TA500349). Positive lysates LY403405 (100ug) and LC403405 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY432053) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229026 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PROM2 protein (Cat# TP311605). The protein was produced from HEK293T cells transfected with PROM2 cDNA clone (Cat# RC211605) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prominin 2
• Synonyms: PROML2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211605 representing NM_144707
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCACACACTGGCTCTGCTGGCTCCCCTGCTGGGCCTGGGCCTGGGGCTGGCCCTGAGTCAGCTGG
  CTGCAGGGGCCACAGACTGCAAGTTCCTTGGCCCGGCAGAGCACCTGACATTCACCCCAGCAGCCAGGGC
  CCGGTGGCTGGCCCCTCGAGTTCGTGCGCCAGGACTCCTGGACTCCCTCTATGGCACCGTGCGCCGCTTC
  CTCTCGGTGGTGCAGCTCAATCCTTTCCCTTCAGAGTTGGTAAAGGCCCTACTGAATGAGCTGGCCTCCG
  TGAAGGTGAATGAGGTGGTGCGGTACGAGGCGGGCTACGTGGTATGCGCTGTGATCGCGGGCCTCTACCT
  GCTGCTGGTGCCCACTGCCGGGCTTTGCTTCTGCTGCTGCCGCTGCCACCGGCGCTGCGGGGGACGAGTG
  AAGACAGAGCACAAGGCGCTGGCCTGTGAGCGCGCGGCCCTCATGGTCTTCCTGCTGCTGACCACCCTCT
  TGCTGCTGATTGGTGTGGTCTGTGCCTTTGTCACCAACCAGCGCACGCATGAACAGATGGGCCCCAGCAT
  CGAGGCCATGCCTGAGACCCTGCTCAGCCTCTGGGGCCTGGTCTCTGATGTCCCCCAAGAGCTGCAGGCC
  GTGGCACAGCAATTCTCCCTGCCCCAGGAGCAAGTCTCAGAGGAGCTGGATGGTGTTGGTGTGAGCATTG
  GGAGCGCGATCCACACTCAGCTCAGGAGCTCCGTGTACCCCTTGCTGGCGGCCGTGGGCAGTTTGGGCCA
  GGTCCTGCAGGTCTCCGTGCACCACCTGCAAACCTTGAATGCTACAGTGGTAGAGCTGCAGGCCGGGCAG
  CAGGACCTGGAGCCAGCCATCCGGGAACACCGGGACCGCCTCCTTGAGCTGCTGCAGGAGGCCAGGTGCC
  AGGGAGATTGTGCAGGGGCCCTGAGCTGGGCCCGCACCCTGGAGCTGGGTGCTGACTTCAGCCAGGTGCC
  CTCTGTGGACCATGTCCTGCACCAGCTAAAAGGTGTCCCCGAGGCCAACTTCTCCAGCATGGTCCAGGAG
  GAGAACAGCACCTTCAACGCCCTTCCAGCCCTGGCTGCCATGCAGACATCCAGCGTGGTGCAAGAGCTGA
  AGAAGGCAGTGGCCCAGCAGCCGGAAGGGGTGAGGACACTGGCTGAAGGGTTCCCGGGCTTGGAGGCAGC
  TTCCCGCTGGGCCCAGGCACTGCAGGAGGTGGAGGAGAGCAGCCGCCCCTACCTGCAGGAGGTGCAGAGA
  TACGAGACCTACAGGTGGATCGTGGGCTGCGTGCTGTGCTCCGTGGTCCTATTCGTGGTGCTCTGCAACC
  TGCTGGGCCTCAATCTGGGCATCTGGGGCCTGTCTGCCAGGGACGACCCCAGCCACCCAGAAGCCAAGGG
  CGAGGCTGGAGCCCGCTTCCTCATGGCAGGTGTGGGCCTCAGCTTCCTCTTTGCTGCACCCCTCATCCTC
  CTGGTGTTCGCCACCTTCCTGGTGGGTGGCAACGTGCAGACGCTGGTGTGCCGGAGCTGGGAGAACGGCG
  AGCTCTTTGAGTTTGCAGACACCCCAGGGAACCTGCCCCCGTCCATGAACCTGTCGCAACTTCTTGGCCT
  GAGGAAGAACATCAGCATCCACCAAGCCTATCAGCAGTGCAAGGAAGGGGCAGCGCTCTGGACAGTCCTG
  CAGCTCAACGACTCCTACGACCTGGAGGAGCACCTGGATATCAACCAGTATACCAACAAGCTACGGCAGG
  AGTTGCAGAGCCTGAAAGTAGACACACAGAGCCTGGACCTGCTGAGCTCAGCCGCCCGCCGGGACCTGGA
  GGCCCTGCAGAGCAGTGGGCTTCAGCGCATCCACTACCCCGACTTCCTCGTTCAGATCCAGAGGCCCGTG
  GTGAAGACCAGCATGGAGCAGCTGGCCCAGGAGCTGCAAGGACTGGCCCAGGCCCAAGACAATTCTGTGC
  TGGGGCAGCGGCTGCAGGAGGAGGCCCAAGGACTCAGAAACCTTCACCAGGAGAAGGTCGTCCCCCAGCA
  GAGCCTTGTGGCAAAGCTCAACCTCAGCGTCAGGGCCCTGGAGTCCTCTGCCCCGAATCTCCAGCTGGAG
  ACCTCAGATGTCCTAGCCAATGTCACCTACCTGAAAGGAGAGCTGCCTGCCTGGGCAGCCAGGATCCTGA
  GGAATGTGAGTGAGTGTTTCCTGGCCCGGGAGATGGGCTACTTCTCCCAGTACGTGGCCTGGGTGAGAGA
  GGAGGTGACTCAGCGCATTGCCACCTGCCAGCCCCTCTCCGGAGCCCTGGACAACAGCCGTGTGATCCTG
  TGTGACATGATGGCTGACCCCTGGAATGCCTTCTGGTTCTGCCTGGCATGGTGCACCTTCTTCCTGATCC
  CCAGCATCATCTTTGCCGTCAAGACCTCCAAATACTTCCGTCCTATCCGGAAACGCCTCAGCTCCACCAG
  CTCTGAGGAGACTCAGCTCTTCCACATCCCCCGGGTTACCTCCCTGAAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211605 representing NM_144707
  Red=Cloning site Green=Tags(s)
  
  MKHTLALLAPLLGLGLGLALSQLAAGATDCKFLGPAEHLTFTPAARARWLAPRVRAPGLLDSLYGTVRRF
  LSVVQLNPFPSELVKALLNELASVKVNEVVRYEAGYVVCAVIAGLYLLLVPTAGLCFCCCRCHRRCGGRV
  KTEHKALACERAALMVFLLLTTLLLLIGVVCAFVTNQRTHEQMGPSIEAMPETLLSLWGLVSDVPQELQA
  VAQQFSLPQEQVSEELDGVGVSIGSAIHTQLRSSVYPLLAAVGSLGQVLQVSVHHLQTLNATVVELQAGQ
  QDLEPAIREHRDRLLELLQEARCQGDCAGALSWARTLELGADFSQVPSVDHVLHQLKGVPEANFSSMVQE
  ENSTFNALPALAAMQTSSVVQELKKAVAQQPEGVRTLAEGFPGLEAASRWAQALQEVEESSRPYLQEVQR
  YETYRWIVGCVLCSVVLFVVLCNLLGLNLGIWGLSARDDPSHPEAKGEAGARFLMAGVGLSFLFAAPLIL
  LVFATFLVGGNVQTLVCRSWENGELFEFADTPGNLPPSMNLSQLLGLRKNISIHQAYQQCKEGAALWTVL
  QLNDSYDLEEHLDINQYTNKLRQELQSLKVDTQSLDLLSSAARRDLEALQSSGLQRIHYPDFLVQIQRPV
  VKTSMEQLAQELQGLAQAQDNSVLGQRLQEEAQGLRNLHQEKVVPQQSLVAKLNLSVRALESSAPNLQLE
  TSDVLANVTYLKGELPAWAARILRNVSECFLAREMGYFSQYVAWVREEVTQRIATCQPLSGALDNSRVIL
  CDMMADPWNAFWFCLAWCTFFLIPSIIFAVKTSKYFRPIRKRLSSTSSEETQLFHIPRVTSLKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144707
• ORF Size: 2502 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144707.1, NP_653308.1
• RefSeq Size: 3822 bp
• RefSeq ORF: 2505 bp
• Locus ID: 150696
• UniProt ID: Q8N271
• Cytogenetics: 2q11.1
• Protein Families: Transmembrane
• MW: 91.7 kDa
• Gene Summary: This gene encodes a member of the prominin family of pentaspan membrane glycoproteins. The encoded protein localizes to basal epithelial cells and may be involved in the organization of plasma membrane microdomains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]