SLCO4C1 (NM_180991) Human Tagged ORF Clone

CAT#: RC211533

SLCO4C1 (Myc-DDK-tagged)-Human solute carrier organic anion transporter family, member 4C1 (SLCO4C1)

Product Images

Circular map for RC211533

• 

  Western blot validation of overexpression lysate (Cat# LY403616) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211533 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLCO4C1
• Synonyms: OATP-H; OATP-M1; OATP4C1; OATPX; PRO2176; SLC21A20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211533 representing NM_180991
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGCGCCAAAGGTATTGAGAACTTGGCTTTTGTCCCCTCCAGCCCAGACATCCTGCGCCGCTTGT
  CTGCGTCGCCCTCCCAAATCGAAGTCTCTGCCTTGTCCTCTGACCCCCAAAGAGAGAATTCTCAGCCACA
  GGAGCTTCAGAAGCCCCAGGAGCCCCAGAAGTCACCAGAGCCATCTCTGCCTTCAGCCCCTCCCAATGTC
  TCCGAAGAGAAGCTCCGGTCACTGTCGCTGTCCGAGTTTGAGGAGGGGTCTTACGGCTGGAGGAACTTCC
  ATCCTCAATGTCTCCAGCGCTGCAACACACCTGGAGGCTTTCTGCTTCACTACTGCCTCTTGGCCGTCAC
  GCAAGGTATTGTAGTTAATGGCCTAGTAAATATTAGCATTTCCACTGTTGAGAAGCGTTATGAAATGAAG
  AGTTCCCTGACTGGCCTGATTTCATCAAGCTACGATATTTCATTCTGTTTGTTGTCTTTATTTGTATCAT
  TCTTTGGTGAAAGAGGACATAAGCCGAGATGGCTTGCATTTGCAGCCTTTATGATTGGACTGGGAGCACT
  TGTATTCTCATTGCCACAATTTTTCAGTGGAGAATATAAATTGGGGTCTCTTTTTGAAGACACTTGTGTA
  ACAACAAGGAATAGCACCAGTTGTACATCTTCAACTTCTTCACTTTCTAACTACTTGTATGTCTTCATCT
  TGGGACAACTATTGCTGGGGGCAGGAGGAACTCCTCTTTATACTCTGGGAACAGCCTTTCTTGATGATTC
  TGTGCCCACACACAAGTCTTCTCTCTATATAGGAACCGGTTATGCTATGTCAATCTTAGGCCCTGCTATT
  GGCTATGTATTGGGAGGACAACTGCTAACCATATACATTGATGTTGCTATGGGAGAAAGCACTGATGTCA
  CTGAGGATGATCCGCGATGGTTGGGAGCTTGGTGGATTGGGTTTCTTCTATCATGGATCTTTGCTTGGTC
  TTTAATAATACCTTTTTCTTGCTTTCCAAAACATTTACCAGGTACAGCAGAAATTCAAGCTGGAAAAACT
  TCCCAGGCTCATCAGAGTAATAGTAATGCAGATGTGAAATTTGGAAAAAGTATTAAAGATTTTCCAGCTG
  CTCTAAAGAATTTGATGAAGAATGCTGTCTTTATGTGTTTAGTTCTATCAACTTCTTCAGAAGCCTTAAT
  TACTACTGGATTTGCTACATTTTTACCTAAATTTATAGAAAATCAATTCGGATTGACATCCAGCTTCGCA
  GCTACTCTTGGAGGGGCTGTTTTAATTCCTGGAGCTGCTCTCGGTCAAATTTTAGGTGGCTTCCTTGTTT
  CAAAATTCAGAATGACATGTAAAAACACAATGAAGTTTGCACTGTTCACATCTGGAGTTGCACTTACGCT
  GAGTTTTGTATTTATGTATGCCAAATGTGAAAATGAGCCATTTGCTGGTGTATCTGAATCATATAATGGG
  ACTGGAGAATTGGGAAACTTGATAGCCCCTTGTAATGCCAATTGTAACTGTTCGCGATCATATTATTATC
  CTGTCTGTGGAGATGGAGTCCAATATTTTTCTCCCTGCTTTGCAGGCTGTTCAAACCCAGTTGCACACAG
  GAAGCCAAAGGTATATTACAACTGTTCCTGTATTGAAAGGAAAACAGAAATAACATCCACTGCAGAAACT
  TTTGGTTTTGAAGCTAAAGCTGGAAAATGTGAAACTCATTGTGCGAAACTGCCCATATTCCTTTGCATTT
  TCTTTATTGTAATTATTTTTACCTTTATGGCCGGTACTCCTATAACTGTGTCTATCCTAAGGTGTGTTAA
  TCACAGACAACGGTCCCTAGCCTTGGGAATACAATTTATGGTCCTTCGATTATTAGGGACAATTCCTGGA
  CCAATTATATTTGGTTTCACAATAGACAGCACATGTATTCTTTGGGATATAAATGATTGTGGAATTAAAG
  GAGCTTGCTGGATTTATGATAACATCAAGATGGCCCATATGCTAGTAGCCATAAGTGTTACTTGTAAAGT
  TATCACCATGTTCTTCAATGGATTTGCAATCTTTTTGTATAAACCACCTCCATCAGCCACAGATGTGTCA
  TTTCATAAAGAGAATGCAGTTGTGACTAATGTTTTAGCAGAACAGGATCTCAACAAAATAGTAAAAGAAG
  GG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211533 representing NM_180991
  Red=Cloning site Green=Tags(s)
  
  MKSAKGIENLAFVPSSPDILRRLSASPSQIEVSALSSDPQRENSQPQELQKPQEPQKSPEPSLPSAPPNV
  SEEKLRSLSLSEFEEGSYGWRNFHPQCLQRCNTPGGFLLHYCLLAVTQGIVVNGLVNISISTVEKRYEMK
  SSLTGLISSSYDISFCLLSLFVSFFGERGHKPRWLAFAAFMIGLGALVFSLPQFFSGEYKLGSLFEDTCV
  TTRNSTSCTSSTSSLSNYLYVFILGQLLLGAGGTPLYTLGTAFLDDSVPTHKSSLYIGTGYAMSILGPAI
  GYVLGGQLLTIYIDVAMGESTDVTEDDPRWLGAWWIGFLLSWIFAWSLIIPFSCFPKHLPGTAEIQAGKT
  SQAHQSNSNADVKFGKSIKDFPAALKNLMKNAVFMCLVLSTSSEALITTGFATFLPKFIENQFGLTSSFA
  ATLGGAVLIPGAALGQILGGFLVSKFRMTCKNTMKFALFTSGVALTLSFVFMYAKCENEPFAGVSESYNG
  TGELGNLIAPCNANCNCSRSYYYPVCGDGVQYFSPCFAGCSNPVAHRKPKVYYNCSCIERKTEITSTAET
  FGFEAKAGKCETHCAKLPIFLCIFFIVIIFTFMAGTPITVSILRCVNHRQRSLALGIQFMVLRLLGTIPG
  PIIFGFTIDSTCILWDINDCGIKGACWIYDNIKMAHMLVAISVTCKVITMFFNGFAIFLYKPPPSATDVS
  FHKENAVVTNVLAEQDLNKIVKEG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_180991
• ORF Size: 2172 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_180991.5
• RefSeq Size: 5334 bp
• RefSeq ORF: 2175 bp
• Locus ID: 353189
• UniProt ID: Q6ZQN7
• Cytogenetics: 5q21.1
• Protein Families: Transmembrane
• MW: 78.8 kDa
• Gene Summary: SLCO4C1 belongs to the organic anion transporter (OATP) family. OATPs are involved in the membrane transport of bile acids, conjugated steroids, thyroid hormone, eicosanoids, peptides, and numerous drugs in many tissues (Mikkaichi et al., 2004 [PubMed 14993604]).[supplied by OMIM, Mar 2008]