HEATR6 (NM_022070) Human Tagged ORF Clone

CAT#: RC211523

HEATR6 (Myc-DDK-tagged)-Human HEAT repeat containing 6 (HEATR6)

Product Images

Circular map for RC211523

• 

  Western blot validation of overexpression lysate (Cat# LY411793) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211523 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified HEATR6 protein (Cat# TP311523). The protein was produced from HEK293T cells transfected with HEATR6 cDNA clone (Cat# RC211523) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HEATR6
• Synonyms: ABC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211523 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTGTGCAAGTTGTCGGTTCGTGGCCTTCCGTGCAGCCGCGGGAGGCACCGCGGGAAGCAATCC
  CTGAGCGAGGCAATGGGTTTCGCCTCTTGTCTGCCAGGCTCTGCGCCCTGCGCCCGGATGACAGCAGCTC
  CGCCCGCACCGAGATCCACCTGCTCTTCGATCAGCTCATCTCCGAGAACTACAGCGAGGGCAGTGGCGTG
  GCCCCGGAGGACGTTAGTGCTCTTCTTGTCCAGGCTTGCCGACTGGTACCTCTTAATCAGAATCATCTTG
  TCAGCAAAGTGAGCCAGCTTATCCACCATTTACTTAACAGATTACAGGTAATTGTTGATGAACAGCACTT
  GGATTTCCTGTTGGCATATACTATTTCGGCTATTCATCAGTGTAGTTCCTGGACACACAGGGAAATTCTT
  CAAGCCCTGGCAGCTCTGGTGTACTGCAATGGCTCCAAATGTCAAAAGTACCTCCCAGAGCTGCTAGGCA
  ACACCGGACTCTTAATGAAGTTGAGTGACTTGGCTCAGTCTGATCCTGAAGTCAGGAGAGCTGCAGTACA
  TTGTATGGCAAACTTATGTCTCAGTGTGCCAGGACAGCCGTATTTGGAGGAGCCCTACCAAAATGTCTGT
  TTCCAAGCTTTTCTGACTATTTTACAGTCTCCAAAATCATCTGATATGGATGATATCACATTTTGCATGT
  TATTGCAAAATGCATTAAAAGGTATACAGTCACTTCTAAATGGTGGGAGAATGAAACTAACACAGACTGA
  TGAACTTGGAGCACTTTTAGCTGTGCTAAAGAAATTCATGTTTCACGGACTCCCTGGACTAAACATAGAG
  ATGCCCACGGTGTTATACCCAACTCCGCTTCCTCAGTATGATGGGCGAACACCTATCAAACCACAGCAAT
  CAGAATCCAGTGCTTCTCGACCAACTTTGAATAAAAAGAAAAAATCCAAAGTAAAACCAAAGAAAATCCA
  GCAAGGAGAGGAGGAGGAAAAGGAATCCAGTGGTGAAATAGAGGCAGCCCCAGTCACTGGCACAGGCAGA
  GTGAACCTGCATGAAGGGAACACTTGGTGTCCCTCCTCCCTGGGTGTCCAGAGTTTGCCTTTAGATGGAA
  GTGGAGCTGCAGAAAAAGATGGAGTCTCCTCATCCTTCAGTTCTTCCAGTTGGAAAAGGGTCAGCAGTAG
  TGAGTCAGACTTTTCTGATGCTGAAGGAGGCATGCAGAGTAAAATGAGGTCTTACCAAGCTAAAGTTCGC
  CAAGGAGCCTTAGTTTGTTTTCTTTCTACTATAAAATCGATAGAAAAAAAAGTTCTTTATGGCTACTGGT
  CAGCTTTTATTCCTGATACGCCTGAACTTGGCAGCCCACAGTCAGTGTCCTTGATGACTCTTACATTGAA
  AGACCCTTCTCCAAAGACACGTGCCTGTGCTCTGCAAGTTTTATCTGCCATCTTGGAAGGCTCAAAGCAG
  TTTCTTTCTGTTGCTGAAGATACCAGTGACCACAGAAGGGCTTTTACCCCCTTCTCCGTAATGATCGCTT
  GCAGCATTAGAGAGTTGCACAGATGTCTTTTGTTAGCTTTGGTGGCGGAGTCATCCTCACAGACCGTTAC
  TCAGATAATTAAGTGCCTTGCAAATTTAGTATCAAATGCACCTTATGATCGTCTAAAACTCAGCCTGCTG
  ACCAAAGTCTGGAACCAGATAAAGCCTTATATTCGCCACAAAGATGTTAATGTTCGTGTGTCAAGTCTCA
  CACTCTTGGGAGCTATAGTGTCCACCCACGCACCTTTACCTGAAGTCCAACTACTTCTGCAACAGCCATG
  TTCTTCTGGACTCGGTAATAGCAATTCAGCAACCCCTCACCTCAGCCCTCCTGATTGGTGGAAGAAAGCC
  CCTGCAGGACCCTCTCTGGAAGAAACGTCAGTTAGCTCACCTAAGGGGTCTTCAGAGCCCTGCTGGCTCA
  TTCGACTCTGCATTTCCATTGTCGTACTGCCCAAGGAGGATTCCTGTTCAGGTAGCGATGCTGGCTCTGC
  AGCAGGAAGCACCTACGAACCATCCCCCATGCGACTGGAGGCCTTACAGGTATTGACTCTTCTGGCAAGG
  GGCTACTTTTCAATGACTCAAGCCTACTTGATGGAGCTTGGAGAGGTGATTTGCAAGTGCATGGGGGAAG
  CAGATCCATCCATTCAGCTTCATGGAGCAAAGCTTCTGGAAGAACTGGGCACAGGCTTAATACAGCAGTA
  TAAACCAGACTCCACTGCAGCACCTGATCAGAGAGCACCAGTCTTCTTGGTGGTGATGTTCTGGACTATG
  ATGCTGAACGGTCCTTTACCCAGAGCCCTGCAGAATTCAGAACACCCAACTCTCCAGGCGAGCGCCTGTG
  ATGCCCTGTCTTCCATCTTGCCAGAGGCCTTCAGCAATCTGCCGAATGACAGGCAGATGCTGTGCATCAC
  AGTGCTGCTCGGGCTGAATGACAGCAAGAATCGCTTAGTGAAAGCTGCAACTTCACGGGCCCTGGGAGTC
  TATGTGCTTTTTCCCTGTCTCAGACAGGATGTCATATTTGTTGCAGACGCAGCAAATGCAATATTGATGT
  CACTTGAAGACAAGTCTCTGAATGTTCGAGCCAAAGCAGCCTGGTCCCTGGGCAACCTGACAGACACTCT
  GATTGTCAACATGGAAACACCAGACCCAAGTTTCCAGGAAGAGTTCTCTGGTCTCCTGCTCTTGAAAATG
  TTACGATCAGCTATAGAAGCATCCAAGGATAAAGACAAGGTAAAAAGCAATGCAGTCCGGGCCCTTGGAA
  ATTTGCTTCATTTTCTGCAACCCTCTCATATAGAAAAACCCACATTTGCAGAAATCATTGAGGAGTCTAT
  CCAGGCCCTAATTTCTACTGTTCTAACAGAAGCTGCCATGAAAGTCCGATGGAATGCTTGTTATGCAATG
  GGAAATGTATTTAAAAATCCTGCCCTTCCTTTAGGGACAGCCCCATGGACCTCCCAGGCCTACAATGCCC
  TGACATCGGTCGTGACATCATGCAAGAACTTCAAAGTGCGCATCAGATCTGCAGCTGCCCTTTCCGTCCC
  GGGGAAGAGAGAGCAGTACGGGTCTGTTGACCAGTATGCTCGGATCTGGAATGCATTGGTCACCGCTTTA
  CAGAAGAGTGAAGACACCATAGACTTTTTGGAATTCAAGTACTGTGTCAGCCTACGGACCCAAATCTGCC
  AGGCACTGATTCACCTCTTGAGCTTGGCCAGTGCCTCGGACCTCCCTTGTATGAAAGAAACCCTTGAACT
  GAGTGGGAATATGGTCCAGTCCTATATTCTACAGTTTTTAAAATCAGGAGCAGAGGGAGATGACACTGGA
  GCACCCCACAGCCCACAGGAAAGAGACCAGATGGTCAGAATGGCCCTTAAACACATGGGCAGCATCCAGG
  CACCAACTGGAGACACAGCCAGAAGGGCCATCATGGGCTTTTTAGAAGAGATCCTGGCCGTTTGTTTTGA
  CTCATCTGGATCACAAGGGGCACTCCCAGGGTTAACAAATCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211523 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAVQVVGSWPSVQPREAPREAIPERGNGFRLLSARLCALRPDDSSSARTEIHLLFDQLISENYSEGSGV
  APEDVSALLVQACRLVPLNQNHLVSKVSQLIHHLLNRLQVIVDEQHLDFLLAYTISAIHQCSSWTHREIL
  QALAALVYCNGSKCQKYLPELLGNTGLLMKLSDLAQSDPEVRRAAVHCMANLCLSVPGQPYLEEPYQNVC
  FQAFLTILQSPKSSDMDDITFCMLLQNALKGIQSLLNGGRMKLTQTDELGALLAVLKKFMFHGLPGLNIE
  MPTVLYPTPLPQYDGRTPIKPQQSESSASRPTLNKKKKSKVKPKKIQQGEEEEKESSGEIEAAPVTGTGR
  VNLHEGNTWCPSSLGVQSLPLDGSGAAEKDGVSSSFSSSSWKRVSSSESDFSDAEGGMQSKMRSYQAKVR
  QGALVCFLSTIKSIEKKVLYGYWSAFIPDTPELGSPQSVSLMTLTLKDPSPKTRACALQVLSAILEGSKQ
  FLSVAEDTSDHRRAFTPFSVMIACSIRELHRCLLLALVAESSSQTVTQIIKCLANLVSNAPYDRLKLSLL
  TKVWNQIKPYIRHKDVNVRVSSLTLLGAIVSTHAPLPEVQLLLQQPCSSGLGNSNSATPHLSPPDWWKKA
  PAGPSLEETSVSSPKGSSEPCWLIRLCISIVVLPKEDSCSGSDAGSAAGSTYEPSPMRLEALQVLTLLAR
  GYFSMTQAYLMELGEVICKCMGEADPSIQLHGAKLLEELGTGLIQQYKPDSTAAPDQRAPVFLVVMFWTM
  MLNGPLPRALQNSEHPTLQASACDALSSILPEAFSNLPNDRQMLCITVLLGLNDSKNRLVKAATSRALGV
  YVLFPCLRQDVIFVADAANAILMSLEDKSLNVRAKAAWSLGNLTDTLIVNMETPDPSFQEEFSGLLLLKM
  LRSAIEASKDKDKVKSNAVRALGNLLHFLQPSHIEKPTFAEIIEESIQALISTVLTEAAMKVRWNACYAM
  GNVFKNPALPLGTAPWTSQAYNALTSVVTSCKNFKVRIRSAAALSVPGKREQYGSVDQYARIWNALVTAL
  QKSEDTIDFLEFKYCVSLRTQICQALIHLLSLASASDLPCMKETLELSGNMVQSYILQFLKSGAEGDDTG
  APHSPQERDQMVRMALKHMGSIQAPTGDTARRAIMGFLEEILAVCFDSSGSQGALPGLTNQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022070
• ORF Size: 3543 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022070.5
• RefSeq Size: 3935 bp
• RefSeq ORF: 3546 bp
• Locus ID: 63897
• UniProt ID: Q6AI08
• Cytogenetics: 17q23.1
• MW: 128.8 kDa
• Gene Summary: Amplification-dependent oncogene.[UniProtKB/Swiss-Prot Function]