SLC9A8 (NM_015266) Human Tagged ORF Clone

CAT#: RC211351

SLC9A8 (Myc-DDK-tagged)-Human solute carrier family 9 (sodium/hydrogen exchanger), member 8 (SLC9A8)

Product Images

Circular map for RC211351

• 

  Western blot validation of overexpression lysate (Cat# LY414657) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211351 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC9A8
• Synonyms: NHE-8; NHE8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211351 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAGAAGATGGCGGAAGAGGAGAGGTTCCCCAATACAACTCATGAGGGTTTCAATGTCACCCTCC
  ACACCACCCTGGTTGTCACGACGAAACTGGTGCTCCCGACCCCTGGCAAGCCCATCCTCCCCGTGCAGAC
  AGGGGAGCAGGCCCAGCAAGAGGAGCAGTCCAGCGGCATGACCATTTTCTTCAGCCTCCTTGTCCTAGCT
  ATCTGCATCATATTGGTGCATTTACTGATCCGATACAGATTACATTTCTTGCCAGAGAGTGTTGCTGTTG
  TTTCTTTAGGTATTCTCATGGGAGCAGTTATAAAAATTATAGAGTTTAAAAAACTGGCGAATTGGAAGGA
  AGAAGAAATGTTTCGTCCAAACATGTTTTTCCTCCTCCTGCTTCCCCCTATTATCTTTGAGTCTGGATAT
  TCATTACACAAGGGTAACTTCTTTCAAAATATTGGTTCCATCACCCTGTTTGCTGTTTTTGGGACGGCAA
  TCTCCGCTTTTGTAGTAGGTGGAGGAATTTATTTTCTGGGTCAGGCTGATGTAATCTCTAAACTCAACAT
  GACAGACAGTTTTGCGTTTGGCTCCCTAATATCTGCTGTCGATCCAGTGGCCACTATTGCCATTTTCAAT
  GCACTTCATGTGGACCCCGTGCTCAACATGCTGGTCTTTGGAGAAAGTATTCTCAACGATGCAGTCTCCA
  TTGTTCTGACCAACACAGCTGAAGGTTTAACAAGAAAAAATATGTCAGATGTCAGTGGGTGGCAAACATT
  TTTACAAGCCCTTGACTACTTCCTCAAAATGTTCTTTGGCTCTGCAGCGCTCGGCACTCTCACTGGCTTA
  ATTTCTGCATTAGTGCTGAAGCATATTGACTTGAGGAAAACGCCTTCCTTGGAGTTTGGCATGATGATCA
  TTTTTGCTTATCTGCCTTATGGGCTTGCAGAAGGAATCTCACTCTCAGGCATCATGGCCATCCTGTTCTC
  AGGCATCGTGATGTCCCACTACACGCACCATAACCTCTCCCCAGTCACCCAGATCCTCATGCAGCAGACC
  CTCCGCACCGTGGCCTTCTTATGTGAAACATGTGTGTTTGCATTTCTTGGCCTGTCCATTTTTAGTTTTC
  CTCACAAGTTTGAAATTTCCTTTGTCATCTGGTGCATAGTGCTTGTACTATTTGGCAGAGCGGTAAACAT
  TTTCCCTCTTTCCTACCTCCTGAATTTCTTCCGGGATCATAAAATCACACCGAAGATGATGTTCATCATG
  TGGTTTAGTGGCCTGCGGGGAGCCATCCCCTATGCCCTGAGCCTACACCTGGACCTGGAGCCCATGGAGA
  AGCGGCAGCTCATCGGCACCACCACCATCGTCATCGTGCTCTTCACCATCCTGCTGCTGGGCGGCAGCAC
  CATGCCCCTCATTCGCCTCATGGACATCGAGGACGCCAAGGCACACCGCAGGAACAAGAAGGACGTCAAC
  CTCAGCAAGACTGAGAAGATGGGCAACACTGTGGAGTCGGAGCACCTGTCGGAGCTCACGGAGGAGGAGT
  ACGAGGCCCACTACATCAGGCGGCAGGACCTTAAGGGCTTCGTGTGGCTGGACGCCAAGTACCTGAACCC
  CTTCTTCACTCGGAGGCTGACGCAGGAGGACCTGCACCACGGGCGCATCCAGATGAAAACTCTCACCAAC
  AAGTGGTACGAGGAGGTACGCCAGGGCCCCTCCGGCTCCGAGGACGACGAGCAGGAGCTGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211351 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGEKMAEEERFPNTTHEGFNVTLHTTLVVTTKLVLPTPGKPILPVQTGEQAQQEEQSSGMTIFFSLLVLA
  ICIILVHLLIRYRLHFLPESVAVVSLGILMGAVIKIIEFKKLANWKEEEMFRPNMFFLLLLPPIIFESGY
  SLHKGNFFQNIGSITLFAVFGTAISAFVVGGGIYFLGQADVISKLNMTDSFAFGSLISAVDPVATIAIFN
  ALHVDPVLNMLVFGESILNDAVSIVLTNTAEGLTRKNMSDVSGWQTFLQALDYFLKMFFGSAALGTLTGL
  ISALVLKHIDLRKTPSLEFGMMIIFAYLPYGLAEGISLSGIMAILFSGIVMSHYTHHNLSPVTQILMQQT
  LRTVAFLCETCVFAFLGLSIFSFPHKFEISFVIWCIVLVLFGRAVNIFPLSYLLNFFRDHKITPKMMFIM
  WFSGLRGAIPYALSLHLDLEPMEKRQLIGTTTIVIVLFTILLLGGSTMPLIRLMDIEDAKAHRRNKKDVN
  LSKTEKMGNTVESEHLSELTEEEYEAHYIRRQDLKGFVWLDAKYLNPFFTRRLTQEDLHHGRIQMKTLTN
  KWYEEVRQGPSGSEDDEQELL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015266
• ORF Size: 1743 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015266.3
• RefSeq Size: 6278 bp
• RefSeq ORF: 1746 bp
• Locus ID: 23315
• UniProt ID: Q9Y2E8
• Cytogenetics: 20q13.13
• Protein Families: Druggable Genome, Transmembrane
• MW: 65.4 kDa
• Gene Summary: Sodium-hydrogen exchangers (NHEs), such as SLC9A8, are integral transmembrane proteins that exchange extracellular Na+ for intracellular H+. NHEs have multiple functions, including intracellular pH homeostasis, cell volume regulation, and electroneutral NaCl absorption in epithelia (Xu et al., 2008 [PubMed 18209477]).[supplied by OMIM, Apr 2009]