Angiopoietin 4 (ANGPT4) (NM_015985) Human Tagged ORF Clone

CAT#: RC211289

ANGPT4 (Myc-DDK-tagged)-Human angiopoietin 4 (ANGPT4)

Product Images

Circular map for RC211289

• 

  Western blot validation of overexpression lysate (Cat# LY414259) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211289 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Angiopoietin 4
• Synonyms: ANG3; ANG4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211289 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTCCCAGCTAGCCATGCTGCAGGGCAGCCTCCTCCTTGTGGTTGCCACCATGTCTGTGGCTCAAC
  AGACAAGGCAGGAGGCGGATAGGGGCTGCGAGACACTTGTAGTCCAGCACGGCCACTGTAGCTACACCTT
  CTTGCTGCCCAAGTCTGAGCCCTGCCCTCCGGGGCCTGAGGTCTCCAGGGACTCCAACACCCTCCAGAGA
  GAATCACTGGCCAACCCACTGCACCTGGGGAAGTTGCCCACCCAGCAGGTGAAACAGCTGGAGCAGGCAC
  TGCAGAACAACACGCAGTGGCTGAAGAAGCTAGAGAGGGCCATCAAGACGATCTTGAGGTCGAAGCTGGA
  GCAGGTCCAGCAGCAAATGGCCCAGAATCAGACGGCCCCCATGCTAGAGCTGGGCACCAGCCTCCTGAAC
  CAGACCACTGCCCAGATCCGCAAGCTGACCGACATGGAGGCTCAGCTCCTGAACCAGACATCAAGAATGG
  ATGCCCAGATGCCAGAGACCTTTCTGTCCACCAACAAGCTGGAGAACCAGCTGCTGCTACAGAGGCAGAA
  GCTCCAGCAGCTTCAGGGCCAAAACAGCGCGCTCGAGAAGCGGTTGCAGGCCCTGGAGACCAAGCAGCAG
  GAGGAGCTGGCCAGCATCCTCAGCAAGAAGGCGAAGCTGCTGAACACGCTGAGCCGCCAGAGCGCCGCCC
  TCACCAACATCGAGCGCGGCCTGCGCGGTGTCAGGCACAACTCCAGCCTCCTGCAGGACCAGCAGCACAG
  CCTGCGCCAGCTGCTGGTGTTGTTGCGGCACCTGGTGCAAGAAAGGGCTAACGCCTCGGCCCCGGCCTTC
  ATAATGGCAGGTGAGCAGGTGTTCCAGGACTGTGCAGAGATCCAGCGCTCTGGGGCCAGTGCCAGTGGTG
  TCTACACCATCCAGGTGTCCAATGCAACGAAGCCCAGGAAGGTGTTCTGTGACCTGCAGAGCAGTGGAGG
  CAGGTGGACCCTCATCCAGCGCCGTGAGAATGGCACCGTGAATTTTCAGCGGAACTGGAAGGATTACAAA
  CAGGGCTTCGGAGACCCAGCTGGGGAGCACTGGCTGGGCAATGAAGTGGTGCACCAGCTCACCAGAAGGG
  CAGCCTACTCTCTGCGTGTGGAGCTGCAAGACTGGGAAGGCCACGAGGCCTATGCCCAGTACGAACATTT
  CCACCTGGGCAGTGAGAACCAGCTATACAGGCTTTCTGTGGTCGGGTACAGCGGCTCAGCAGGGCGCCAG
  AGCAGCCTGGTCCTGCAGAACACCAGCTTTAGCACCCTTGACTCAGACAACGACCACTGTCTCTGCAAGT
  GTGCCCAAGTGATGTCTGGAGGGTGGTGGTTTGACGCCTGTGGCCTGTCAAACCTCAACGGCGTCTACTA
  CCACGCTCCCGACAACAAGTACAAGATGGACGGCATCCGCTGGCACTACTTCAAGGGCCCCAGCTACTCA
  CTGCGTGCCTCTCGCATGATGATACGGCCTTTGGACATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211289 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLSQLAMLQGSLLLVVATMSVAQQTRQEADRGCETLVVQHGHCSYTFLLPKSEPCPPGPEVSRDSNTLQR
  ESLANPLHLGKLPTQQVKQLEQALQNNTQWLKKLERAIKTILRSKLEQVQQQMAQNQTAPMLELGTSLLN
  QTTAQIRKLTDMEAQLLNQTSRMDAQMPETFLSTNKLENQLLLQRQKLQQLQGQNSALEKRLQALETKQQ
  EELASILSKKAKLLNTLSRQSAALTNIERGLRGVRHNSSLLQDQQHSLRQLLVLLRHLVQERANASAPAF
  IMAGEQVFQDCAEIQRSGASASGVYTIQVSNATKPRKVFCDLQSSGGRWTLIQRRENGTVNFQRNWKDYK
  QGFGDPAGEHWLGNEVVHQLTRRAAYSLRVELQDWEGHEAYAQYEHFHLGSENQLYRLSVVGYSGSAGRQ
  SSLVLQNTSFSTLDSDNDHCLCKCAQVMSGGWWFDACGLSNLNGVYYHAPDNKYKMDGIRWHYFKGPSYS
  LRASRMMIRPLDI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015985
• ORF Size: 1509 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015985.4
• RefSeq Size: 1955 bp
• RefSeq ORF: 1512 bp
• Locus ID: 51378
• UniProt ID: Q9Y264
• Cytogenetics: 20p13
• Protein Families: Druggable Genome, Secreted Protein
• MW: 56.8 kDa
• Gene Summary: Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The mechanism by which they contribute to angiogenesis is thought to involve regulation of endothelial cell interactions with supporting perivascular cells. The protein encoded by this gene functions as an agonist and is an angiopoietin. [provided by RefSeq, Jul 2008]