SMUBP2 (IGHMBP2) (NM_002180) Human Tagged ORF Clone

CAT#: RC211261

IGHMBP2 (Myc-DDK-tagged)-Human immunoglobulin mu binding protein 2 (IGHMBP2)

Product Images

Circular map for RC211261

• 

  Western blot validation of overexpression lysate (Cat# LY419482) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211261 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SMUBP2
• Synonyms: CATF1; CMT2S; HCSA; HMN6; SMARD1; SMUBP2; ZFAND7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211261 representing NM_002180
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGGCAGCTGTGGAGAGCTTCGTGACCAAGCAACTGGACCTGCTGGAGCTCGAGAGAGACGCGG
  AGGTGGAGGAGCGCAGGTCCTGGCAGGAGAACATCTCTCTGAAAGAGCTCCAGAGCCGAGGCGTGTGTTT
  GCTGAAGCTGCAGGTATCCAGCCAGCGCACTGGGCTGTACGGACGGCTGCTGGTCACCTTTGAGCCCAGG
  CGATACGGGTCCGCGGCAGCTCTTCCCAGTAACAGCTTTACTTCTGGTGATATCGTGGGCCTGTACGATG
  CTGCTAATGAGGGCAGTCAGCTGGCCACTGGGATCTTGACCCGGGTCACCCAGAAGTCGGTCACGGTGGC
  CTTTGATGAGTCCCACGATTTCCAGTTGAGCTTGGACCGAGAGAATTCCTACAGACTGTTAAAACTTGCC
  AATGATGTCACTTACAGGCGACTGAAAAAAGCCCTGATTGCTCTAAAGAAGTATCATTCTGGCCCAGCCT
  CCTCACTCATAGAAGTGCTCTTTGGCAGATCTGCTCCCAGTCCTGCCAGTGAAATACACCCGCTGACATT
  CTTCAACACCTGCCTGGACACCTCCCAGAAAGAAGCGGTTTCATTTGCGCTGTCTCAGAAAGAACTTGCC
  ATCATCCATGGACCTCCTGGCACTGGGAAAACCACGACTGTGGTTGAGATCATTCTTCAAGCTGTGAAAC
  AAGGCTTAAAGGTTCTGTGCTGCGCCCCCTCCAACATCGCCGTGGACAATCTGGTGGAGCGCCTGGCTCT
  GTGTAAGCAGCGGATTCTGCGCCTGGGACACCCTGCCCGCCTCCTGGAGTCCATTCAGCAGCACTCCCTG
  GATGCGGTTTTAGCGCGGAGCGACAGTGCCCAGATTGTTGCAGATATCAGGAAGGACATCGACCAGGTCT
  TTGTGAAAAACAAAAAGACCCAGGATAAGAGAGAGAAAAGTAATTTTCGAAATGAAATTAAGCTGTTAAG
  AAAAGAACTGAAGGAGAGGGAAGAAGCAGCTATGCTCGAGAGCCTCACTTCGGCAAACGTGGTCCTTGCA
  ACAAACACAGGTGCGTCTGCCGATGGCCCCCTGAAGTTGCTGCCCGAGAGCTACTTCGACGTGGTGGTCA
  TTGACGAGTGTGCCCAGGCCCTCGAGGCGAGCTGCTGGATCCCCCTGCTGAAGGCCAGAAAGTGCATCCT
  GGCGGGCGATCACAAGCAGCTGCCCCCCACCACAGTCTCTCACAAGGCTGCGCTGGCAGGACTGTCACTC
  AGCCTGATGGAACGCCTGGCTGAGGAGTACGGCGCGAGGGTGGTGCGGACACTGACGGTGCAGTACCGCA
  TGCACCAGGCTATCATGCGCTGGGCCTCAGACACCATGTACCTTGGGCAGCTCACAGCCCACTCTTCCGT
  GGCAAGGCACCTCCTGAGGGACCTCCCAGGTGTGGCTGCCACAGAAGAGACGGGTGTGCCCCTGCTCTTG
  GTGGACACCGCCGGCTGCGGGCTGTTTGAGCTGGAGGAGGAGGACGAACAGTCGAAAGGGAACCCTGGCG
  AAGTCCGCCTCGTCAGTTTGCACATCCAGGCTCTGGTGGACGCTGGTGTTCCAGCCCGTGACATTGCTGT
  GGTCTCGCCATACAACCTCCAGGTGGACCTGCTCAGACAGAGCCTTGTGCACAGGCACCCTGAGCTTGAA
  ATCAAGTCTGTCGATGGCTTCCAAGGCCGAGAGAAGGAGGCCGTGATACTGTCCTTCGTCAGATCCAACA
  GGAAAGGTGAAGTTGGTTTTCTTGCTGAGGACCGGAGGATCAACGTGGCTGTCACCCGTGCCCGACGCCA
  CGTGGCGGTCATCTGTGACTCCCGTACTGTCAACAACCATGCATTTTTGAAGACCCTGGTGGAGTATTTC
  ACACAGCATGGGGAAGTACGCACGGCCTTTGAGTATCTTGACGATATTGTCCCAGAAAACTATTCCCATG
  AGAACTCCCAGGGTTCCAGCCACGCTGCCACCAAGCCCCAGGGACCTGCTGCGTCCACCAGGACCGGAAG
  CCAGCGGCAGGAGGGAGGCCAGGAGGCTGCAGCACCTGCCAGACAGGGCCGGAAGAAGCCGGCTGGGAAG
  TCTCTGGCCTCTGAAGCTCCATCTCAGCCCAGCCTCAACGGAGGCAGCCCAGAGGGAGTGGAGAGCCAAG
  ATGGCGTGGACCACTTCCGGGCCATGATAGTGGAGTTCATGGCCAGCAAGAAGATGCAGTTGGAGTTTCC
  TCCTTCCCTCAATTCCCACGACAGGCTGCGGGTCCACCAAATAGCCGAGGAGCACGGGCTGAGGCACGAC
  AGTTCTGGGGAAGGGAAGAGGAGGTTCATCACTGTGAGCAAGAGGGCCCCGCGACCCCGAGCAGCCCTGG
  GACCCCCAGCAGGGACCGGTGGCCCAGCCCCTCTCCAGCCAGTGCCCCCTACCCCTGCGCAGACAGAGCA
  GCCTCCCAGGGAGCAGCGTGGCCCAGACCAGCCTGATCTGAGGACGCTGCACCTGGAGAGACTGCAGAGG
  GTCAGGAGCGCGCAGGGGCAGCCCGCCAGCAAGGAGCAGCAGGCCTCAGGGCAGCAGAAACTTCCAGAAA
  AGAAAAAGAAAAAAGCCAAAGGACATCCGGCCACAGATCTGCCCACGGAGGAGGACTTTGAGGCCCTGGT
  TTCTGCCGCCGTTAAGGCTGATAACACCTGCGGCTTTGCCAAGTGCACAGCCGGCGTCACAACCCTGGGC
  CAGTTCTGCCAGCTCTGCAGCCGCCGCTACTGCCTCAGCCACCACCTGCCCGAGATCCATGGCTGCGGTG
  AGAGGGCTCGCGCCCATGCCCGGCAGAGAATCAGCCGGGAAGGGGTCCTCTATGCCGGCAGCGGGACCAA
  GAACGGATCCCTGGACCCAGCCAAGAGGGCCCAGCTGCAGAGGAGGCTGGATAAGAAGCTGAGTGAGCTC
  AGCAACCAGAGGACCAGCCGGAGGAAGGAGAGGGGGACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211261 representing NM_002180
  Red=Cloning site Green=Tags(s)
  
  MASAAVESFVTKQLDLLELERDAEVEERRSWQENISLKELQSRGVCLLKLQVSSQRTGLYGRLLVTFEPR
  RYGSAAALPSNSFTSGDIVGLYDAANEGSQLATGILTRVTQKSVTVAFDESHDFQLSLDRENSYRLLKLA
  NDVTYRRLKKALIALKKYHSGPASSLIEVLFGRSAPSPASEIHPLTFFNTCLDTSQKEAVSFALSQKELA
  IIHGPPGTGKTTTVVEIILQAVKQGLKVLCCAPSNIAVDNLVERLALCKQRILRLGHPARLLESIQQHSL
  DAVLARSDSAQIVADIRKDIDQVFVKNKKTQDKREKSNFRNEIKLLRKELKEREEAAMLESLTSANVVLA
  TNTGASADGPLKLLPESYFDVVVIDECAQALEASCWIPLLKARKCILAGDHKQLPPTTVSHKAALAGLSL
  SLMERLAEEYGARVVRTLTVQYRMHQAIMRWASDTMYLGQLTAHSSVARHLLRDLPGVAATEETGVPLLL
  VDTAGCGLFELEEEDEQSKGNPGEVRLVSLHIQALVDAGVPARDIAVVSPYNLQVDLLRQSLVHRHPELE
  IKSVDGFQGREKEAVILSFVRSNRKGEVGFLAEDRRINVAVTRARRHVAVICDSRTVNNHAFLKTLVEYF
  TQHGEVRTAFEYLDDIVPENYSHENSQGSSHAATKPQGPAASTRTGSQRQEGGQEAAAPARQGRKKPAGK
  SLASEAPSQPSLNGGSPEGVESQDGVDHFRAMIVEFMASKKMQLEFPPSLNSHDRLRVHQIAEEHGLRHD
  SSGEGKRRFITVSKRAPRPRAALGPPAGTGGPAPLQPVPPTPAQTEQPPREQRGPDQPDLRTLHLERLQR
  VRSAQGQPASKEQQASGQQKLPEKKKKKAKGHPATDLPTEEDFEALVSAAVKADNTCGFAKCTAGVTTLG
  QFCQLCSRRYCLSHHLPEIHGCGERARAHARQRISREGVLYAGSGTKNGSLDPAKRAQLQRRLDKKLSEL
  SNQRTSRRKERGT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002180
• ORF Size: 2979 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002180.3
• RefSeq Size: 3955 bp
• RefSeq ORF: 2982 bp
• Locus ID: 3508
• UniProt ID: P38935
• Cytogenetics: 11q13.3
• Domains: ZnF_AN1, R3H, DEAD, AAA
• Protein Families: Transcription Factors
• MW: 109 kDa
• Gene Summary: This gene encodes a helicase superfamily member that binds a specific DNA sequence from the immunoglobulin mu chain switch region. Mutations in this gene lead to spinal muscle atrophy with respiratory distress type 1. [provided by RefSeq, Jul 2008]