ZIM2 (NM_015363) Human Tagged ORF Clone

CAT#: RC211188

ZIM2 (Myc-DDK-tagged)-Human zinc finger, imprinted 2 (ZIM2), transcript variant 1

Product Images

Circular map for RC211188

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ZIM2 (Cat# RC211188, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZIM2(Cat# TA501906). Positive lysates LY402427 (100ug) and LC402427 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY431969) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228942 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZIM2
• Synonyms: ZNF656
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211188 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCAACCAGAAGACGACAACAACAGTGACGTGACCAGTGACGACGACATGACCCGGAACAGAAGAG
  AGTCCTCACCACCTCACTCAGTCCATTCTTTCAGTGGTGACCGGGACTGGGACCGGAGGGGCAGAAGCAG
  AGACATGGAGCCACGAGACCGCTGGTCCCACACCAGGAACCCAAGAAGCAGGATGCCTCCGCGGGATCTT
  TCCCTTCCTGTGGTGGCGAAAACAAGCTTTGAAATGGACAGAGAGGACGACAGGGACTCCAGGGCTTATG
  AGTCCCGATCTCAGGATGCTGAATCATACCAAAATGTGGTGGACCTCGCTGAGGACAGGAAACCTCACAA
  CACAATCCAGGACAACATGGAAAACTACAGGAAGCTGCTCTCCCTCGGTTTCCTTGCTCAGGACTCTGTC
  CCTGCAGAAAAGAGGAACACAGAGATGTTAGACAATCTGCCATCTGCTGGGTCCCAGTTCCCGGACTTCA
  AACACTTAGGAACATTTCTGGTGTTTGAGGAGTTGGTGACCTTCGAGGATGTGCTTGTGGACTTCAGCCC
  AGAGGAACTTAGCTCCCTTAGTGCTGCTCAGAGAAACCTCTACAGGGAGGTGATGCTGGAGAATTACCGG
  AACCTGGTCTCCCTGGGGCACCAGTTCTCTAAACCTGACATTATCTCACGCCTGGAAGAGGAGGAATCAT
  ATGCAATGGAGACAGACAGCAGACATACAGTGATTTGTCAAGGAGAGTCTCATGATGATCCATTGGAACC
  ACACCAGGGCAACCAAGAGAAACTTTTGACTCCTATAACAATGAATGACCCCAAGACCCTCACTCCGGAA
  AGAAGCTATGGCAGTGATGAATTTGAGAGAAGCTCTAATCTTAGTAAACAATCAAAGGATCCTCTAGGAA
  AGGATCCCCAGGAAGGCACTGCTCCTGGAATATGTACGAGTCCCCAGTCAGCATCCCAAGAGAACAAACA
  CAACAGATGTGAATTTTGCAAACGAACCTTTAGTACGCAAGTAGCCCTTAGGAGACACGAACGGATCCAT
  ACTGGGAAGAAACCCTATGAATGTAAACAGTGTGCTGAAGCCTTCTATCTCATGCCACACCTCAACAGAC
  ATCAGAAGACCCATTCTGGTAGGAAGACTTCTGGCTGCAATGAAGGTAGAAAGCCTTCCGTCCAGTGTGC
  GAATCTCTGTGAACGTGTAAGAATTCACAGTCAGGAGGACTACTTTGAATGTTTTCAGTGCGGCAAAGCT
  TTTCTCCAGAATGTGCATCTTCTTCAACATCTCAAAGCCCATGAGGCAGCAAGAGTCCTTCCTCCTGGGT
  TGTCCCACAGCAAGACATACTTAATTCGTTATCAGCGGAAACATGACTACGTTGGAGAGAGAGCCTGCCA
  GTGTTGTGACTGTGGCAGAGTCTTCAGTCGGAATTCATATCTCATTCAGCATTATAGAACTCACACTCAA
  GAGAGGCCTTACCAGTGTCAGCTATGTGGGAAATGTTTCGGCCGACCCTCATACCTCACTCAACATTATC
  AACTCCATTCTCAAGAGAAAACTGTTGAGTGCGATCACTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211188 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYQPEDDNNSDVTSDDDMTRNRRESSPPHSVHSFSGDRDWDRRGRSRDMEPRDRWSHTRNPRSRMPPRDL
  SLPVVAKTSFEMDREDDRDSRAYESRSQDAESYQNVVDLAEDRKPHNTIQDNMENYRKLLSLGFLAQDSV
  PAEKRNTEMLDNLPSAGSQFPDFKHLGTFLVFEELVTFEDVLVDFSPEELSSLSAAQRNLYREVMLENYR
  NLVSLGHQFSKPDIISRLEEEESYAMETDSRHTVICQGESHDDPLEPHQGNQEKLLTPITMNDPKTLTPE
  RSYGSDEFERSSNLSKQSKDPLGKDPQEGTAPGICTSPQSASQENKHNRCEFCKRTFSTQVALRRHERIH
  TGKKPYECKQCAEAFYLMPHLNRHQKTHSGRKTSGCNEGRKPSVQCANLCERVRIHSQEDYFECFQCGKA
  FLQNVHLLQHLKAHEAARVLPPGLSHSKTYLIRYQRKHDYVGERACQCCDCGRVFSRNSYLIQHYRTHTQ
  ERPYQCQLCGKCFGRPSYLTQHYQLHSQEKTVECDHC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015363
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015363.5
• RefSeq Size: 2113 bp
• RefSeq ORF: 1584 bp
• Locus ID: 23619
• UniProt ID: Q9NZV7
• Cytogenetics: 19q13.43
• Domains: LER, zf-C2H2
• Protein Families: Transcription Factors
• MW: 61.2 kDa
• Gene Summary: In human, ZIM2 and PEG3 (GeneID:5178) are two distinct genes that share a set of 5' exons and have a common promoter, and both genes are paternally expressed. Alternative splicing events connect the shared exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. This is in contrast to mouse and cow, where ZIM2 and PEG3 genes do not share exons in common, and the imprinting status of ZIM2 is also not conserved amongst mammals. Additional 5' alternatively spliced transcripts encoding the same protein have been found for the human ZIM2 gene. [provided by RefSeq, Oct 2010]