TNFSF9 (NM_003811) Human Tagged ORF Clone
TNFSF9 (Myc-DDK-tagged)-Human tumor necrosis factor (ligand) superfamily, member 9 (TNFSF9)
|Type||Human Tagged ORF Clone|
|Synonyms||4-1BB-L; CD137L; TNLG5A|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC211160 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC211160 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||762 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1680 bp|
|RefSeq ORF||765 bp|
|Protein Families||Druggable Genome, Transmembrane|
|Protein Pathways||Cytokine-cytokine receptor interaction|
|Gene Summary||The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.[provided by RefSeq, Oct 2008]|
|cDNA Clone Resources|
|RC211160L1||Lenti ORF clone of Human tumor necrosis factor (ligand) superfamily, member 9 (TNFSF9), Myc-DDK-tagged||
|RC211160L2||Lenti ORF clone of Human tumor necrosis factor (ligand) superfamily, member 9 (TNFSF9), mGFP tagged||
|RC211160L3||Lenti ORF clone of Human tumor necrosis factor (ligand) superfamily, member 9 (TNFSF9), Myc-DDK-tagged||
|RC211160L4||Lenti ORF clone of Human tumor necrosis factor (ligand) superfamily, member 9 (TNFSF9), mGFP tagged||
|RG211160||TNFSF9 (GFP-tagged) - Human tumor necrosis factor (ligand) superfamily, member 9 (TNFSF9)||
|SC303383||TNFSF9 (untagged)-Human tumor necrosis factor (ligand) superfamily, member 9 (TNFSF9)||