Importin4 (IPO4) (NM_024658) Human Tagged ORF Clone

CAT#: RC211049

IPO4 (Myc-DDK-tagged)-Human importin 4 (IPO4)

Product Images

Circular map for RC211049

• 

  Western blot validation of overexpression lysate (Cat# LY411174) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211049 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified IPO4 protein (Cat# TP311049). The protein was produced from HEK293T cells transfected with IPO4 cDNA clone (Cat# RC211049) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Importin4
• Synonyms: Imp4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211049 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCAGCCGGGCTAGAGCAGCTCCTACGGGAGCTGCTGCTACCGGACACCGAGCGCATCCGTCGGG
  CCACGGAACAGCTCCAGATCGTTCTTCGGGCCCCCGCCGCTTTGCCGGCTCTCTGCGACCTGCTAGCCTC
  GGCGGCCGACCCCCAGATCCGCCAGTTTGCGGCCGTGCTGACCCGCAGACGACTGAACACCCGCTGGCGA
  CGGCTGGCGGCGGAGCAACGGGAGAGCCTCAAGTCCCTGATCCTGACGGCCCTGCAGAGAGAAACAGAGC
  ACTGTGTGAGCCTCAGCCTGGCCCAGCTCTCAGCCACCATTTTTCGAAAGGAAGGCTTGGAGGCCTGGCC
  ACAGCTTTTGCAGCTGCTTCAGCACAGTACCCACAGCCCCCACAGCCCAGAGAGAGAGATGGGGCTTTTG
  CTGCTAAGTGTGGTGGTGACCTCCCGGCCCGAGGCCTTCCAACCCCACCACCGGGAGCTTCTTCGGCTTC
  TGAATGAGACTCTTGGTGAGGTGGGCTCTCCTGGGCTGCTCTTCTACTCCCTGCGCACTCTGACCACCAT
  GGCTCCCTACCTCAGCACTGAAGATGTGCCTCTCGCTCGGATGTTGGTGCCCAAGCTGATCATGGCCATG
  CAGACTCTGATCCCCATAGATGAGGCAAAGGCCTGTGAGGCCCTTGAGGCTTTGGATGAACTGTTGGAGT
  CAGAGGTGCCGGTCATCACCCCCTACCTCTCTGAAGTCCTCACATTCTGCCTGGAGGTAGCTAGAAATGT
  GGCCCTGGGCAATGCGATACGCATACGTATTCTCTGCTGCCTCACTTTCTTGGTCAAAGTCAAGAGCAAG
  GCCTTACTGAAGAATCGTCTCCTGCCACCCTTGCTGCACACCCTTTTCCCCATTGTGGCTGCTGAGCCCC
  CACCAGGCCAGTTGGATCCCGAGGACCAGGATTCAGAAGAGGAAGAGTTGGAGATTGAGCTGATGGGGGA
  GACTCCCAAGCATTTCGCTGTACAAGTTGTGGACATGCTGGCACTACACCTGCCCCCCGAGAAGCTCTGT
  CCCCAGCTGATGCCCATGTTGGAAGAGGCTTTGCGGAGCGAGAGCCCATACCAGCGCAAAGCTGGACTCC
  TGGTGCTGGCCGTGCTGTCTGACGGAGCTGGCGACCACATCAGGCAGAGACTGCTGCCCCCACTGCTGCA
  GATTGTGTGCAAGGGCCTGGAGGACCCCTCGCAAGTTGTACGCAATGCTGCGCTGTTTGCCCTGGGCCAG
  TTCTCAGAAAACCTACAGCCCCATATCAGCAGCTATTCAAGGGAGGTAATGCCACTGCTCCTCGCCTACT
  TGAAGTCGGTGCCTCTTGGACACACACACCACCTAGCCAAGGCCTGCTATGCCCTGGAGAATTTTGTGGA
  GAACCTAGGGCCCAAGGTGCAGCCCTACCTTCCGGAGCTTATGGAATGCATGCTGCAGCTTCTGAGGAAC
  CCCAGCAGTCCCCGGGCCAAGGAGCTGGCTGTGAGCGCCCTGGGAGCCATTGCTACGGCTGCCCAGGCCT
  CGCTGCTGCCCTACTTCCCTGCCATCATGGAGCACCTGCGGGAATTCCTGTTAACAGGCCGTGAGGACCT
  TCAGCCTGTGCAGATCCAGAGCCTGGAGACACTGGGGGTGCTGGCACGAGCAGTGGGGGAGCCCATGAGG
  CCGCTGGCTGAGGAATGCTGCCAGCTGGGTCTGGGCCTCTGCGACCAGGTAGACGACCCTGACTTGCGGC
  GCTGCACGTACAGCCTATTTGCAGCCTTATCGGGTCTGATGGGTGAGGGCCTGGCGCCCCACTTGGAACA
  GATCACCACGCTCATGCTGCTGTCACTGCGTTCCACCGAGGGCATTGTGCCTCAGTATGACGGGAGCAGC
  TCCTTCCTTCTGTTTGACGATGAGAGTGATGGGGAAGAAGAGGAGGAGCTCATGGATGAGGATGTGGAAG
  AAGAGGATGACTCAGAGATCTCAGGGTACAGCGTGGAGAATGCCTTCTTCGATGAGAAGGAAGACACCTG
  TGCTGCCGTGGGGGAGATCTCTGTGAACACCAGTGTGGCCTTCCTTCCATACATGGAAAGTGTCTTTGAA
  GAAGTATTTAAACTGCTGGAGTGCCCTCACCTGAATGTGCGGAAGGCAGCCCATGAGGCTCTGGGTCAGT
  TTTGCTGTGCACTGCACAAGGCCTGTCAAAGCTGCCCCTCGGAACCCAACACTGCTGCTTTGCAGGCTGC
  CCTGGCCCGAGTCGTGCCATCCTACATGCAGGCAGTGAACAGGGAGCGGGAACGCCAGGTGGTGATGGCC
  GTGCTGGAGGCCCTGACAGGGGTGCTCCGCAGCTGTGGGACCCTCACACTGAAGCCCCCTGGGCGCCTCG
  CTGAGCTCTGTGGCGTGCTCAAGGCTGTGCTGCAGAGGAAGACAGCCTGTCAGGATACTGACGAGGAGGA
  GGAAGAGGAAGATGATGATCAGGCTGAATACGACGCCATGTTGCTGGAGCACGCTGGAGAGGCCATCCCT
  GCCCTGGCAGCCGCGGCTGGGGGAGACTCCTTTGCCCCATTCTTTGCCGGTTTCCTGCCATTATTGGTGT
  GCAAGACAAAACAGGGCTGCACAGTGGCAGAGAAGTCCTTTGCAGTGGGGACCTTGGCAGAGACTATTCA
  GGGCCTGGGTGCTGCCTCAGCCCAGTTTGTGTCTCGGCTGCTCCCTGTGCTGTTGAGCACCGCCCAAGAG
  GCAGACCCCGAGGTGCGAAGCAATGCCATCTTCGGGATGGGCGTGCTGGCAGAGCATGGGGGCCACCCTG
  CCCAGGAACACTTCCCCAAGCTGCTGGGGCTCCTTTTTCCCCTCCTGGCGCGGGAGCGACATGATCGTGT
  CCGTGACAACATCTGTGGGGCACTTGCCCGCCTGTTGATGGCCAGTCCCACCAGGAAACCAGAGCCCCAG
  GTGCTGGCTGCCCTACTGCATGCCCTGCCACTGAAGGAGGACTTGGAGGAGTGGGTCACCATTGGGCGCC
  TCTTCAGCTTCCTGTACCAGAGCAGCCCTGACCAGGTTATAGATGTGGCTCCCGAGCTTCTGCGTATCTG
  CAGCCTCATTCTGGCTGACAACAAGATCCCACCAGACACCAAGGCCGCACTGTTGCTGCTCCTGACGTTC
  CTGGCCAAACAGCACACCGACAGCTTTCAAGCAGCTCTGGGCTCACTGCCTGTTGACAAGGCTCAGGAGC
  TCCAGGCTGTACTGGGCCTCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211049 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MESAGLEQLLRELLLPDTERIRRATEQLQIVLRAPAALPALCDLLASAADPQIRQFAAVLTRRRLNTRWR
  RLAAEQRESLKSLILTALQRETEHCVSLSLAQLSATIFRKEGLEAWPQLLQLLQHSTHSPHSPEREMGLL
  LLSVVVTSRPEAFQPHHRELLRLLNETLGEVGSPGLLFYSLRTLTTMAPYLSTEDVPLARMLVPKLIMAM
  QTLIPIDEAKACEALEALDELLESEVPVITPYLSEVLTFCLEVARNVALGNAIRIRILCCLTFLVKVKSK
  ALLKNRLLPPLLHTLFPIVAAEPPPGQLDPEDQDSEEEELEIELMGETPKHFAVQVVDMLALHLPPEKLC
  PQLMPMLEEALRSESPYQRKAGLLVLAVLSDGAGDHIRQRLLPPLLQIVCKGLEDPSQVVRNAALFALGQ
  FSENLQPHISSYSREVMPLLLAYLKSVPLGHTHHLAKACYALENFVENLGPKVQPYLPELMECMLQLLRN
  PSSPRAKELAVSALGAIATAAQASLLPYFPAIMEHLREFLLTGREDLQPVQIQSLETLGVLARAVGEPMR
  PLAEECCQLGLGLCDQVDDPDLRRCTYSLFAALSGLMGEGLAPHLEQITTLMLLSLRSTEGIVPQYDGSS
  SFLLFDDESDGEEEEELMDEDVEEEDDSEISGYSVENAFFDEKEDTCAAVGEISVNTSVAFLPYMESVFE
  EVFKLLECPHLNVRKAAHEALGQFCCALHKACQSCPSEPNTAALQAALARVVPSYMQAVNRERERQVVMA
  VLEALTGVLRSCGTLTLKPPGRLAELCGVLKAVLQRKTACQDTDEEEEEEDDDQAEYDAMLLEHAGEAIP
  ALAAAAGGDSFAPFFAGFLPLLVCKTKQGCTVAEKSFAVGTLAETIQGLGAASAQFVSRLLPVLLSTAQE
  ADPEVRSNAIFGMGVLAEHGGHPAQEHFPKLLGLLFPLLARERHDRVRDNICGALARLLMASPTRKPEPQ
  VLAALLHALPLKEDLEEWVTIGRLFSFLYQSSPDQVIDVAPELLRICSLILADNKIPPDTKAALLLLLTF
  LAKQHTDSFQAALGSLPVDKAQELQAVLGLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024658
• ORF Size: 3243 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024658.4
• RefSeq Size: 3606 bp
• RefSeq ORF: 3246 bp
• Locus ID: 79711
• UniProt ID: Q8TEX9
• Cytogenetics: 14q12
• Domains: IBN_NT, HEAT_PBS
• Protein Families: Druggable Genome
• MW: 118.7 kDa
• Gene Summary: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.[UniProtKB/Swiss-Prot Function]