NFATC2IP (NM_032815) Human Tagged ORF Clone

CAT#: RC211004

NFATC2IP (Myc-DDK-tagged)-Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein (NFATC2IP)

Product Images

Circular map for RC211004

• 

  Western blot validation of overexpression lysate (Cat# LY409942) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211004 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NFATC2IP
• Synonyms: ESC2; NIP45; RAD60
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211004 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCCTGTGGGGAAGCGGGGCCGCTGGTCCGGAGGTAGCGGTGCCGGCCGAGGGGGTCGGGGCG
  GCTGGGGCGGTCGGGGCCGGCGTCCTCGGGCCCAGCGGTCTCCATCCCGGGGCACGCTGGACGTAGTGTC
  TGTGGACTTGGTCACCGACAGCGATGAGGAAATTCTGGAGGTCGCCACCGCTCGCGGTGCCGCGGACGAG
  GTTGAGGTGGAGCCCCCGGAGCCCCCGGGGCCGGTCGCGTCCCGGGATAACAGCAACAGTGACAGCGAAG
  GGGAGGACAGGCGGCCCGCAGGACCCCCGCGGGAGCCGGTCAGGCGGCGGCGGCGGCTGGTGCTGGATCC
  GGGGGAGGCGCCGCTGGTTCCGGTGTACTCGGGGAAGGTTAAAAGCAGCCTTCGCCTTATCCCAGATGAT
  CTATCCCTCCTGAAACTCTACCCTCCAGGGGATGAGGAAGAGGCAGAGCTGGCAGATTCGAGTGGTCTCT
  ACCATGAGGGCTCCCCATCACCAGGCTCTCCCTGGAAGACAAAGCTGAGGACTAAGGATAAAGAAGAGAA
  GAAAAAGACAGAGTTTCTGGATCTGGACAACTCTCCTCTGTCCCCACCTTCACCAAGGACCAAAAGCAGA
  ACGCATACTCGGGCACTCAAGAAGTTAAGTGAGGTGAACAAGCGCCTCCAGGATCTCCGTTCCTGTCTGA
  GCCCCAAGCCACCTCAGGGTCAAGAGCAACAGGGCCAAGAGGATGAAGTGGTCTTGGTGGAAGGGCCCAC
  CCTCCCAGAGACCCCCCGACTCTTCCCACTCAAAATCCGTTGCCGGGCTGACCTGGTCAGATTGCCCCTC
  AGGATGTCGGAGCCCCTGCAGAGTGTGGTGGACCACATGGCCACCCACCTTGGGGTGTCCCCAAGCAGGA
  TCCTTTTGCTTTTTGGAGAGACAGAGCTATCACCTACTGCCACTCCCAGGACCCTAAAGCTCGGAGTGGC
  TGACATCATTGACTGTGTGGTACTAACAAGTTCTCCAGAGGCCACAGAGACGTCCCAACAGCTCCAGCTC
  CGGGTGCAGGGAAAGGAGAAACACCAGACACTGGAAGTCTCACTGTCTCGAGATTCCCCTCTAAAGACCC
  TCATGTCCCACTATGAGGAGGCCATGGGACTGTCGGGACGGAAGCTCTCCTTCTTCTTTGATGGGACAAA
  GCTTTCAGGCAGGGAGCTGCCAGCTGACCTGGGCATGGAATCTGGGGACCTCATTGAGGTCTGGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211004 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEPVGKRGRWSGGSGAGRGGRGGWGGRGRRPRAQRSPSRGTLDVVSVDLVTDSDEEILEVATARGAADE
  VEVEPPEPPGPVASRDNSNSDSEGEDRRPAGPPREPVRRRRRLVLDPGEAPLVPVYSGKVKSSLRLIPDD
  LSLLKLYPPGDEEEAELADSSGLYHEGSPSPGSPWKTKLRTKDKEEKKKTEFLDLDNSPLSPPSPRTKSR
  THTRALKKLSEVNKRLQDLRSCLSPKPPQGQEQQGQEDEVVLVEGPTLPETPRLFPLKIRCRADLVRLPL
  RMSEPLQSVVDHMATHLGVSPSRILLLFGETELSPTATPRTLKLGVADIIDCVVLTSSPEATETSQQLQL
  RVQGKEKHQTLEVSLSRDSPLKTLMSHYEEAMGLSGRKLSFFFDGTKLSGRELPADLGMESGDLIEVWG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032815
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032815.3, NP_116204.3
• RefSeq Size: 3877 bp
• RefSeq ORF: 1260 bp
• Locus ID: 84901
• UniProt ID: Q8NCF5
• Cytogenetics: 16p11.2
• MW: 45.8 kDa
• Gene Summary: In T-helper 2 (Th2) cells, regulates the magnitude of NFAT-driven transcription of a specific subset of cytokine genes, including IL3, IL4, IL5 and IL13, but not IL2. Recruits PRMT1 to the IL4 promoter; this leads to enhancement of histone H4 'Arg-3'-methylation and facilitates subsequent histone acetylation at the IL4 locus, thus promotes robust cytokine expression (By similarity). Down-regulates formation of poly-SUMO chains by UBE2I/UBC9 (By similarity).[UniProtKB/Swiss-Prot Function]