ZSCAN4 (NM_152677) Human Tagged ORF Clone

CAT#: RC211003

ZSCAN4 (Myc-DDK-tagged)-Human zinc finger and SCAN domain containing 4 (ZSCAN4)

Product Images

Circular map for RC211003

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ZSCAN4 (Cat# RC211003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZSCAN4(Cat# TA800448). Positive lysates LY407355 (100ug) and LC407355 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY407355) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211003 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ZSCAN4 protein (Cat# TP311003). The protein was produced from HEK293T cells transfected with ZSCAN4 cDNA clone (Cat# RC211003) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZSCAN4
• Synonyms: ZNF494
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211003 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTAGATCTAAGAACCATATTTCAGTGTGAACCATCCGAGAATAATCTTGGATCAGAAAATTCAG
  CGTTTCAACAAAGCCAAGGACCTGCTGTTCAGAGAGAAGAAGGGATTTCTGAGTTCTCAAGAATGGTGCT
  CAATTCATTTCAAGACAGCAATAATTCATATGCAAGGCAGGAATTGCAAAGACTTTATAGGATCTTTCAC
  TCATGGCTGCAACCAGAAAAGCACAGCAAGGATGAAATTATTTCTCTATTAGTCCTGGAGCAGTTTATGA
  TTGGTGGCCACTGCAATGACAAAGCCAGTGTGAAAGAGAAATGGAAATCAAGTGGCAAAAACTTGGAGAG
  ATTCATAGAAGACCTGACTGATGACAGCATAAATCCACCTGCCTTAGTCCACGTCCACATGCAGGGACAG
  GAAGCTCTCTTTTCTGAGGATATGCCCTTAAGAGATGTCATTGTTCATCTCACAAAACAAGTGAATGCCC
  AAACCACAAGAGAAGCAAACATGGGGACACCCTCCCAGACTTCCCAAGATACTTCCTTAGAAACAGGACA
  AGGATATGAAGATGAACAAGATGGCTGGAACAGTTCTTCGAAAACTACTCGAGTAAATGAAAATATTACT
  AATCAAGGCAATCAAATAGTTTCCCTAATCATCATCCAGGAAGAGAACGGTCCTAGGCCTGAAGAGGGAG
  GTGTTTCTTCTGACAACCCATACAACTCAAAAAGAGCAGAGCTAGTCACTGCTAGATCTCAGGAAGGGTC
  CATAAATGGAATCACTTTCCAAGGTGTCCCTATGGTGATGGGAGCAGGGTGTATCTCTCAACCAGAGCAG
  TCCTCCCCTGAGTCTGCCCTTACCCACCAGAGCAATGAGGGAAATTCCACATGTGAGGTACATCAGAAAG
  GATCCCATGGAGTCCAAAAATCATACAAATGTGAAGAATGCCCCAAGGTCTTTAAGTATCTCTGTCACTT
  ATTAGCTCACCAGAGAAGACACAGGAATGAGAGGCCATTTGTTTGTCCCGAGTGTCAAAAAGGCTTCTTC
  CAGATATCAGACCTACGGGTGCATCAGATAATTCACACAGGAAAGAAGCCTTTCACATGCAGCATGTGTA
  AAAAGTCCTTCAGCCACAAAACCAACCTGCGGTCTCATGAGAGAATCCACACAGGAGAAAAGCCTTATAC
  ATGTCCCTTTTGTAAGACAAGCTACCGCCAGTCATCCACATACCACCGCCATATGAGGACTCATGAGAAA
  ATTACCCTGCCAAGTGTTCCCTCCACACCAGAAGCTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211003 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALDLRTIFQCEPSENNLGSENSAFQQSQGPAVQREEGISEFSRMVLNSFQDSNNSYARQELQRLYRIFH
  SWLQPEKHSKDEIISLLVLEQFMIGGHCNDKASVKEKWKSSGKNLERFIEDLTDDSINPPALVHVHMQGQ
  EALFSEDMPLRDVIVHLTKQVNAQTTREANMGTPSQTSQDTSLETGQGYEDEQDGWNSSSKTTRVNENIT
  NQGNQIVSLIIIQEENGPRPEEGGVSSDNPYNSKRAELVTARSQEGSINGITFQGVPMVMGAGCISQPEQ
  SSPESALTHQSNEGNSTCEVHQKGSHGVQKSYKCEECPKVFKYLCHLLAHQRRHRNERPFVCPECQKGFF
  QISDLRVHQIIHTGKKPFTCSMCKKSFSHKTNLRSHERIHTGEKPYTCPFCKTSYRQSSTYHRHMRTHEK
  ITLPSVPSTPEAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152677
• ORF Size: 1299 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152677.2, NP_689890.1
• RefSeq Size: 2260 bp
• RefSeq ORF: 1302 bp
• Locus ID: 201516
• UniProt ID: Q8NAM6
• Cytogenetics: 19q13.43
• Protein Families: Transcription Factors
• MW: 49 kDa
• Gene Summary: The ZSCAN4 gene encodes a protein involved in telomere maintenance and with a key role in the critical feature of mouse embryonic stem (ES) cells, namely, defying cellular senescence and maintaining normal karyotype for many cell divisions in culture (Zalzman et al., 2010 [PubMed 20336070]).[supplied by OMIM, May 2010]