GIRK2 (KCNJ6) (NM_002240) Human Tagged ORF Clone

CAT#: RC210938

KCNJ6 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 6 (KCNJ6)

Product Images

Circular map for RC210938

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  Western blot validation of overexpression lysate (Cat# LY400812) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210938 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KCNJ6 protein (Cat# TP310938). The protein was produced from HEK293T cells transfected with KCNJ6 cDNA clone (Cat# RC210938) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GIRK2
• Synonyms: BIR1; GIRK-2; GIRK2; hiGIRK2; KATP-2; KATP2; KCNJ7; KIR3.2; KPLBS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210938 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGCTGACAGAATCCATGACTAACGTCCTGGAGGGCGACTCCATGGATCAGGACGTCGAAAGCC
  CAGTGGCCATTCACCAGCCAAAGTTGCCTAAGCAGGCCAGGGATGACCTGCCAAGACACATCAGCCGAGA
  TCGGACCAAAAGGAAAATCCAGAGGTACGTGAGGAAAGACGGAAAGTGCAATGTTCATCACGGCAACGTG
  AGGGAGACCTATCGCTACCTGACCGATATCTTCACCACATTAGTGGACCTGAAGTGGAGATTCAACCTAT
  TGATTTTTGTCATGGTTTACACAGTGACCTGGCTCTTTTTTGGAATGATCTGGTGGTTGATCGCATACAT
  ACGGGGAGACATGGACCACATAGAGGACCCCTCCTGGACTCCTTGTGTTACCAACCTCAACGGGTTCGTC
  TCTGCTTTTTTATTCTCAATAGAGACAGAAACCACCATTGGTTATGGCTACCGGGTCATCACAGATAAAT
  GCCCGGAGGGAATTATTCTTCTCTTAATCCAATCTGTGTTGGGGTCCATTGTCAATGCATTCATGGTGGG
  ATGCATGTTTGTAAAAATCTCTCAACCCAAGAAGAGGGCAGAGACCCTGGTCTTTTCCACCCATGCAGTG
  ATCTCCATGCGGGATGGGAAACTGTGCCTGATGTTCCGGGTAGGGGACCTTAGGAATTCCCACATTGTGG
  AGGCTTCCATCAGAGCCAAGTTGATCAAATCCAAACAGACCTCGGAGGGGGAGTTCATCCCGTTGAACCA
  GACGGATATCAACGTAGGGTATTACACGGGGGATGACCGTCTGTTTCTGGTGTCACCGCTGATCATTAGC
  CATGAAATTAACCAACAGAGTCCTTTCTGGGAGATCTCCAAAGCCCAGCTGCCCAAAGAGGAACTGGAAA
  TTGTGGTCATCCTAGAAGGAATGGTGGAAGCCACAGGGATGACATGCCAAGCTCGAAGCTCCTACATCAC
  CAGTGAGATCCTGTGGGGTTACCGGTTCACACCTGTCCTGACCCTGGAGGACGGGTTCTACGAAGTTGAC
  TACAACAGCTTCCATGAGACCTATGAGACCAGCACCCCATCCCTTAGTGCCAAAGAGCTGGCCGAGTTAG
  CCAGCAGGGCAGAGCTGCCCCTGAGTTGGTCTGTATCCAGCAAACTCAACCAACATGCAGAACTGGAGAC
  TGAAGAGGAAGAAAAGAACCTCGAAGAGCAAACAGAAAGAAATGGTGATGTGGCAAACCTGGAGAATGAA
  TCCAAAGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210938 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAKLTESMTNVLEGDSMDQDVESPVAIHQPKLPKQARDDLPRHISRDRTKRKIQRYVRKDGKCNVHHGNV
  RETYRYLTDIFTTLVDLKWRFNLLIFVMVYTVTWLFFGMIWWLIAYIRGDMDHIEDPSWTPCVTNLNGFV
  SAFLFSIETETTIGYGYRVITDKCPEGIILLLIQSVLGSIVNAFMVGCMFVKISQPKKRAETLVFSTHAV
  ISMRDGKLCLMFRVGDLRNSHIVEASIRAKLIKSKQTSEGEFIPLNQTDINVGYYTGDDRLFLVSPLIIS
  HEINQQSPFWEISKAQLPKEELEIVVILEGMVEATGMTCQARSSYITSEILWGYRFTPVLTLEDGFYEVD
  YNSFHETYETSTPSLSAKELAELASRAELPLSWSVSSKLNQHAELETEEEEKNLEEQTERNGDVANLENE
  SKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002240
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002240.5
• RefSeq Size: 2537 bp
• RefSeq ORF: 1272 bp
• Locus ID: 3763
• UniProt ID: P48051
• Cytogenetics: 21q22.13
• Domains: IRK
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 48.5 kDa
• Gene Summary: This gene encodes a member of the G protein-coupled inwardly-rectifying potassium channel family of inward rectifier potassium channels. This type of potassium channel allows a greater flow of potassium into the cell than out of it. These proteins modulate many physiological processes, including heart rate in cardiac cells and circuit activity in neuronal cells, through G-protein coupled receptor stimulation. Mutations in this gene are associated with Keppen-Lubinsky Syndrome, a rare condition characterized by severe developmental delay, facial dysmorphism, and intellectual disability. [provided by RefSeq, Apr 2015]