STYK1 (NM_018423) Human Tagged ORF Clone

CAT#: RC210846

STYK1 (Myc-DDK-tagged)-Human serine/threonine/tyrosine kinase 1 (STYK1)

Product Images

Circular map for RC210846

• 

  Western blot validation of overexpression lysate (Cat# LY402684) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210846 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified STYK1 protein (Cat# TP310846). The protein was produced from HEK293T cells transfected with STYK1 cDNA clone (Cat# RC210846) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STYK1
• Synonyms: NOK; SuRTK106
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210846 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATGACACGGATGCTCCTGGAATGCAGTCTCAGTGACAAGTTGTGTGTCATCCAGGAGAAGCAGT
  ATGAAGTGATTATCGTCCCAACTTTGTTGGTTACTATCTTCCTCATCCTTCTTGGGGTCATCCTGTGGCT
  TTTTATCAGAGAACAAAGAACTCAACAGCAGCGTTCTGGACCTCAAGGCATTGCCCCTGTTCCTCCACCT
  AGGGACCTAAGCTGGGAAGCAGGACATGGAGGAAATGTGGCTTTGCCACTTAAGGAGACATCCGTGGAAA
  ACTTTCTGGGAGCTACCACACCTGCCCTGGCTAAGCTGCAGGTGCCGCGGGAGCAACTCTCTGAAGTTCT
  GGAGCAGATTTGCAGTGGTAGCTGTGGGCCCATCTTTCGAGCCAATATGAACACTGGGGACCCTTCTAAG
  CCCAAGAGTGTTATTCTCAAGGCTTTAAAAGAACCAGCTGGGCTCCATGAGGTACAAGATTTCTTAGGGC
  GAATCCAATTCCATCAATACCTGGGGAAACACAAAAACCTGGTGCAGCTGGAAGGCTGCTGCACTGAAAA
  GCTGCCACTCTATATGGTGTTGGAGGATGTGGCCCAGGGGGACCTGCTCGGCTTTCTCTGGACCTGTCGG
  CGGGATGTGATGACTATGGATGGTCTTCTCTATGATCTCACAGAAAAACAAGTATATCACATCGGAAAGC
  AGGTCCTTTTGGCGCTGGAATTCCTGCAGGAGAAGCATTTGTTCCATGGGGATGTGGCAGCCAGGAATAT
  TCTGATGCAAAGTGATCTCACTGCTAAGCTCTGTGGATTAGGCCTGGCTTATGAAGTTTACACCCGAGGG
  GCCATCTCCTCTACTCAAACCATACCTCTCAAGTGGCTTGCCCCAGAACGGCTTCTCCTGAGACCTGCTA
  GCATCAGAGCAGATGTCTGGTCTTTTGGGATCCTGCTCTATGAGATGGTGACTCTAGGAGCACCACCGTA
  TCCTGAAGTCCCTCCTACCAGCATCCTAGAGCATCTCCAAAGAAGGAAAATCATGAAGAGACCCAGTAGC
  TGCACACATACCATGTACAGTATCATGAAGTCCTGCTGGCGCTGGCGTGAGGCTGACCGCCCCTCACCTA
  GAGAGCTGCGCTTGCGCCTAGAAGCTGCCATTAAAACTGCAGATGACGAGGCTGTGTTACAAGTACCAGA
  GTTGGTGGTACCTGAACTGTATGCAGCTGTGGCCGGCATCAGAGTGGAGAGCCTCTTCTACAACTATAGC
  ATGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210846 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGMTRMLLECSLSDKLCVIQEKQYEVIIVPTLLVTIFLILLGVILWLFIREQRTQQQRSGPQGIAPVPPP
  RDLSWEAGHGGNVALPLKETSVENFLGATTPALAKLQVPREQLSEVLEQICSGSCGPIFRANMNTGDPSK
  PKSVILKALKEPAGLHEVQDFLGRIQFHQYLGKHKNLVQLEGCCTEKLPLYMVLEDVAQGDLLGFLWTCR
  RDVMTMDGLLYDLTEKQVYHIGKQVLLALEFLQEKHLFHGDVAARNILMQSDLTAKLCGLGLAYEVYTRG
  AISSTQTIPLKWLAPERLLLRPASIRADVWSFGILLYEMVTLGAPPYPEVPPTSILEHLQRRKIMKRPSS
  CTHTMYSIMKSCWRWREADRPSPRELRLRLEAAIKTADDEAVLQVPELVVPELYAAVAGIRVESLFYNYS
  ML
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018423
• ORF Size: 1266 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018423.1, NP_060893.1
• RefSeq Size: 3022 bp
• RefSeq ORF: 1269 bp
• Locus ID: 55359
• UniProt ID: Q6J9G0
• Cytogenetics: 12p13.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• MW: 47.5 kDa
• Gene Summary: Receptor protein tyrosine kinases, like STYK1, play important roles in diverse cellular and developmental processes, such as cell proliferation, differentiation, and survival (Liu et al., 2004 [PubMed 15150103]).[supplied by OMIM, Mar 2008]