uronyl 2 sulfotransferase (UST) (NM_005715) Human Tagged ORF Clone

CAT#: RC210799

UST (Myc-DDK-tagged)-Human uronyl-2-sulfotransferase (UST)

Product Images

Circular map for RC210799

• 

  Western blot validation of overexpression lysate (Cat# LY401743) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210799 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: uronyl 2 sulfotransferase
• Synonyms: 2OST
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210799 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAGAAGCAGCAGCATCCCGGCGGCGGCGCGGATCCCTGGCCCCATGGGGCCCCTATGGGGGGCG
  CCCCTCCGGGCCTGGGCAGCTGGAAGCGTCGGGTGCCCCTGCTGCCTTTCCTGCGCTTCTCCCTCCGGGA
  CTACGGCTTCTGCATGGCCACCCTGCTGGTCTTCTGCCTGGGCTCCCTCCTCTATCAGCTCAGCGGGGGA
  CCCCCTCGCTTCCTGCTCGACCTGCGGCAGTACTTGGGAAATTCCACTTACTTGGATGACCATGGACCAC
  CTCCTAGTAAGGTACTACCTTTCCCAAGCCAGGTGGTGTACAACAGGGTAGGCAAGTGTGGGAGCCGTAC
  TGTGGTCTTGCTTCTGAGAATCTTGTCGGAGAAGCACGGATTTAATTTGGTCACATCAGACATTCACAAC
  AAAACCAGGCTTACTAAAAATGAACAAATGGAACTGATTAAAAATATAAGTACTGCCGAACAACCCTATT
  TATTCACTCGACATGTTCATTTCCTCAACTTCTCAAGGTTTGGAGGAGACCAGCCTGTCTACATCAACAT
  CATTAGAGACCCCGTCAACCGGTTCTTATCCAACTATTTTTTCCGTCGCTTTGGAGACTGGAGAGGGGAA
  CAAAATCACATGATCCGCACCCCCAGCATGAGGCAGGAGGAGCGCTACCTGGATATCAATGAGTGTATTC
  TTGAAAACTATCCCGAGTGCTCCAACCCCAGGTTATTTTACATCATTCCGTACTTTTGTGGACAGCATCC
  CAGATGCAGGGAGCCTGGTGAATGGGCCCTTGAGAGAGCAAAGCTGAACGTGAATGAAAACTTCCTGCTC
  GTGGGGATTCTTGAAGAGTTGGAAGATGTGCTGCTGTTACTGGAAAGATTTTTACCTCATTACTTCAAGG
  GCGTGCTCAGTATCTACAAAGACCCAGAGCACAGGAAGCTTGGAAACATGACTGTGACGGTGAAGAAGAC
  TGTCCCCTCTCCTGAGGCTGTGCAGATCCTCTACCAGCGGATGAGATACGAGTACGAGTTTTACCACTAC
  GTCAAAGAGCAGTTCCACCTGCTGAAGCGCAAGTTTGGACTTAAGTCTCACGTCAGCAAGCCCCCCCTGA
  GGCCACACTTCTTTATCCCAACTCCACTGGAAACCGAGGAGCCAATCGACGATGAAGAACAGGATGATGA
  AAAGTGGCTGGAAGATATTTATAAGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210799 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKKKQQHPGGGADPWPHGAPMGGAPPGLGSWKRRVPLLPFLRFSLRDYGFCMATLLVFCLGSLLYQLSGG
  PPRFLLDLRQYLGNSTYLDDHGPPPSKVLPFPSQVVYNRVGKCGSRTVVLLLRILSEKHGFNLVTSDIHN
  KTRLTKNEQMELIKNISTAEQPYLFTRHVHFLNFSRFGGDQPVYINIIRDPVNRFLSNYFFRRFGDWRGE
  QNHMIRTPSMRQEERYLDINECILENYPECSNPRLFYIIPYFCGQHPRCREPGEWALERAKLNVNENFLL
  VGILEELEDVLLLLERFLPHYFKGVLSIYKDPEHRKLGNMTVTVKKTVPSPEAVQILYQRMRYEYEFYHY
  VKEQFHLLKRKFGLKSHVSKPPLRPHFFIPTPLETEEPIDDEEQDDEKWLEDIYKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005715
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005715.3
• RefSeq Size: 4396 bp
• RefSeq ORF: 1221 bp
• Locus ID: 10090
• UniProt ID: Q9Y2C2
• Cytogenetics: 6q25.1
• Domains: HS2ST
• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis
• MW: 47.7 kDa
• Gene Summary: Uronyl 2-sulfotransferase transfers sulfate to the 2-position of uronyl residues, such as iduronyl residues in dermatan sulfate and glucuronyl residues in chondroitin sulfate (Kobayashi et al., 1999 [PubMed 10187838]).[supplied by OMIM, Mar 2008]