ZNF585B (NM_152279) Human Tagged ORF Clone

CAT#: RC210774

ZNF585B (Myc-DDK-tagged)-Human zinc finger protein 585B (ZNF585B)

Product Images

Circular map for RC210774

• 

  Western blot validation of overexpression lysate (Cat# LY407699) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210774 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF585B
• Synonyms: SZFP41; Zfp27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210774 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCTAGTTGGACCTCACCCCAGAAATCCTCAGCCCTGGCTCCAGAGGATCATGGCAGCTCCTATG
  AGGGATCAGTGTCCTTCAGGGATGTGGCTATCGATTTCAGCAGAGAGGAATGGCGGCACCTGGACCTTTC
  TCAGAGAAACCTGTACCGGGATGTGATGCTGGAGACCTACAGCCACCTGCTCTCAGTAGGGTATCAAGTT
  CCTAAACCAGAGGTGGTCATGTTGGAGCAAGGAAAGGAACCATGGGCACTGCAGGGTGAGAGGCCACGTC
  ACAGCTGCCCAGGAGAGAAATTATGGGACCATAATCAACATAGAAAAATCATCGGTTATAAACCAGCTTC
  CTCTCAAGATCAAAAAATTTATTCTGGGGAAAAATCCTATGAGTGTGCTGAATTTGGAAAGAGCTTCACC
  TGGAAGTCACAGTTCAAGGTACATCTGAAAGTTCCTACAGGAGAAAAACTCTATGTATGTATTGAATGTG
  GGAGGGCTTTTGTACAGAAGCCAGAATTCATCACACATCAGAAAACCCATATGAGAGAGAAGCCCTATAA
  GTGCAATGAATGTGGAAAATCCTTTTTTCAAGTATCGTCTCTTTTCAGGCATCACAGAATTCATACCGGA
  GAAAAACTATATGAATGTAGTGAATGTGGGAAAGGTTTCCCTTATAACTCAGATCTCAGTATACATGAGA
  AAATTCATACTGGAGAGAGACACCATGAATGCACTGACTGTGGCAAAGCGTTCACACAAAAGTCCACACT
  CAAGATTCATCAGAAAATCCATACAGGCGAGAGATCCTACATCTGTATTGAATGCGGGCAGGCCTTCATC
  CAGAAAACACAATTGATTGCACACCGAAGAATTCATAGTGGAGAAAAACCATATGAATGCAATAACTGTG
  GCAAATCCTTCATTTCCAAGTCACAACTTCAGGTACATCAACGTGTTCACACAAGAGTGAAGCCCTATAT
  ATGTACCGAATATGGGAAGGTCTTCAGCAATAATTCCAACCTCATTACACATGAGAAGATTCAAAGTAGA
  GAGAAATCTTCCATATGTACTGAGTGTGGGAAGGCCTTTACCTACAGGTCAGAGTTGATTATTCATCAGA
  GAATTCACACTGGAGAGAAACCTTATGAATGCAGTGACTGTGGGAGAGCCTTCACTCAGAAGTCAGCACT
  CACAGTGCATCAGAGAATTCATACAGGAGAAAAATCGTATATATGCATGAAATGTGGACTGGCCTTCATC
  CGGAAGGCACACTTGATTACACATCAAATAATTCATACTGGAGAGAAACCTTATAAATGTGGTCACTGTG
  GGAAATTGTTTACTTCCAAGTCACAACTCCATGTTCATAAACGAATTCACACAGGAGAAAAGCCCTATGT
  ATGCAATAAATGTGGGAAGGCATTCACCAACCGGTCAAATCTCATTACACATCAGAAAACTCATACAGGA
  GAGAAATCTTATATATGTTCCAAATGTGGAAAGGCCTTCACCCAGAGGTCAGACTTGATTACACATCAGA
  GAATCCATACTGGGGAGAAGCCTTATGAATGCAATACTTGTGGAAAAGCCTTTACTCAGAAGTCAAATCT
  TAATATACATCAGAAAATTCACACTGGAGAGAGACAGTATGAATGCCACGAATGTGGGAAAGCCTTCAAC
  CAGAAATCAATACTCATTGTTCATCAGAAAATTCATACAGGAGAGAAACCCTATGTATGCACTGAGTGTG
  GAAGAGCTTTCATCCGCAAGTCAAACTTTATTACTCATCAAAGAATTCATACTGGAGAGAAGCCTTATGA
  ATGCAGTGACTGTGGGAAGTCCTTTACCTCCAAGTCTCAGCTCCTGGTGCATCAGCCAGTTCACACAGGA
  GAGAAACCCTATGTGTGTGCCGAGTGTGGGAAAGCCTTTAGTGGCAGGTCAAATCTCAGTAAGCACCAGA
  AAACTCATACCGGAGAAAAGCCCTACATTTGTTCTGAATGTGGGAAGACCTTTCGACAGAAGTCAGAGTT
  GATTACACATCACAGAATTCATACTGGAGAGAAACCTTATGAGTGCAGTGACTGTGGGAAGTCTTTCACT
  AAAAAATCACAGCTCCAAGTGCATCAGCGAATTCACACTGGAGAGAAGCCTTACGTGTGTGCTGAGTGTG
  GGAAGGCCTTTAGCAACAGGTCCAATTTGAATAAACATCAGACAACACACACTGGAGACAAACCCTACAA
  GTGTGGCATCTGTGGGAAAGGCTTCGTTCAGAAATCAGTGTTCAGTGTTCATCAGAGCAGCCACGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210774 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPASWTSPQKSSALAPEDHGSSYEGSVSFRDVAIDFSREEWRHLDLSQRNLYRDVMLETYSHLLSVGYQV
  PKPEVVMLEQGKEPWALQGERPRHSCPGEKLWDHNQHRKIIGYKPASSQDQKIYSGEKSYECAEFGKSFT
  WKSQFKVHLKVPTGEKLYVCIECGRAFVQKPEFITHQKTHMREKPYKCNECGKSFFQVSSLFRHHRIHTG
  EKLYECSECGKGFPYNSDLSIHEKIHTGERHHECTDCGKAFTQKSTLKIHQKIHTGERSYICIECGQAFI
  QKTQLIAHRRIHSGEKPYECNNCGKSFISKSQLQVHQRVHTRVKPYICTEYGKVFSNNSNLITHEKIQSR
  EKSSICTECGKAFTYRSELIIHQRIHTGEKPYECSDCGRAFTQKSALTVHQRIHTGEKSYICMKCGLAFI
  RKAHLITHQIIHTGEKPYKCGHCGKLFTSKSQLHVHKRIHTGEKPYVCNKCGKAFTNRSNLITHQKTHTG
  EKSYICSKCGKAFTQRSDLITHQRIHTGEKPYECNTCGKAFTQKSNLNIHQKIHTGERQYECHECGKAFN
  QKSILIVHQKIHTGEKPYVCTECGRAFIRKSNFITHQRIHTGEKPYECSDCGKSFTSKSQLLVHQPVHTG
  EKPYVCAECGKAFSGRSNLSKHQKTHTGEKPYICSECGKTFRQKSELITHHRIHTGEKPYECSDCGKSFT
  KKSQLQVHQRIHTGEKPYVCAECGKAFSNRSNLNKHQTTHTGDKPYKCGICGKGFVQKSVFSVHQSSHA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152279
• ORF Size: 2307 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152279.4
• RefSeq Size: 6210 bp
• RefSeq ORF: 2310 bp
• Locus ID: 92285
• UniProt ID: Q52M93
• Cytogenetics: 19q13.12
• Domains: KRAB, zf-C2H2
• Protein Families: Transcription Factors
• MW: 88.1 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]