TRMT5 (NM_020810) Human Tagged ORF Clone

CAT#: RC210671

TRMT5 (Myc-DDK-tagged)-Human TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) (TRMT5)

Product Images

Circular map for RC210671

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TRMT5 (Cat# RC210671, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRMT5 (Cat# TA809321)(1:2000). Positive lysates LY412281 (100ug) and LC412281 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY412281) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210671 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRMT5
• Synonyms: COXPD26; KIAA1393; TRM5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210671 representing NM_020810
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTTTGGATCTTATGGAGGCCATTTGGATTCTCAGGAAGATTTCTGAAACTGGAAAGCCATAGCA
  TAACTGAATCAAAATCGTTGATTCCAGTAGCTTGGACATCCCTGACACAGATGCTTTTGGAAGCACCTGG
  TATTTTCTTATTGGGTCAAAGAAAAAGATTCTCAACCATGCCAGAAACAGAAACACATGAGAGAGAGACT
  GAATTGTTTTCACCACCTTCTGATGTCCGAGGCATGACAAAACTTGATAGAACAGCTTTTAAAAAGACAG
  TCAACATTCCAGTGCTTAAAGTGAGGAAAGAAATAGTCAGTAAATTGATGCGATCCCTAAAAAGGGCAGC
  ATTGCAGCGCCCAGGCATAAGACGTGTGATTGAAGATCCGGAAGATAAAGAAAGTAGACTAATCATGTTG
  GATCCCTATAAAATATTTACTCATGATTCCTTTGAGAAAGCAGAACTCAGTGTTTTAGAGCAGCTTAATG
  TCAGTCCACAGATCTCTAAATACAATTTGGAACTAACATATGAACACTTTAAGTCAGAAGAAATCTTGAG
  AGCTGTGCTTCCTGAAGGTCAAGATGTAACTTCAGGGTTTAGCAGGATTGGACATATTGCACACCTAAAC
  CTTCGAGATCATCAGCTGCCTTTCAAACATTTAATTGGCCAGGTTATGATTGACAAAAATCCAGGAATCA
  CCTCAGCAGTAAATAAAATAAATAATATTGACAATATGTACCGAAATTTCCAAATGGAAGTGCTATCTGG
  AGAGCAGAACATGATGACAAAGGTTCGAGAAAACAACTACACCTATGAATTTGATTTTTCAAAAGTCTAT
  TGGAATCCTCGTCTGTCTACAGAACACAGCCGTATCACAGAACTTCTCAAACCTGGGGATGTCCTATTTG
  ATGTTTTTGCTGGGGTTGGGCCCTTTGCCATTCCAGTAGCAAAGAAAAACTGCACTGTATTTGCCAATGA
  TCTCAATCCTGAATCTCATAAATGGCTGTTGTACAACTGTAAATTAAATAAAGTGGACCAAAAGGTGAAA
  GTCTTCAACTTGGATGGGAAAGACTTCCTCCAAGGACCAGTCAAAGAAGAGTTAATGCAGCTGCTGGGTC
  TGTCAAAAGAAAGAAAACCCTCTGTGCACGTTGTCATGAACTTGCCAGCAAAAGCTATAGAGTTTCTTAG
  TGCTTTCAAGTGGCTTTTAGATGGGCAGCCATGCAGCAGTGAGTTCCTTCCCATAGTGCATTGTTATAGC
  TTTTCCAAAGATGCTAACCCTGCTGAGGATGTTCGGCAAAGGGCTGGAGCTGTGTTAGGCATTTCTCTGG
  AGGCATGCAGTTCAGTTCACCTGGTAAGAAATGTGGCCCCAAACAAGGAAATGCTGTGCATCACGTTTCA
  GATTCCTGCCTCTGTCCTCTACAAGAACCAGACCAGAAATCCAGAGAATCATGAAGATCCACCTCTTAAA
  AGGCAGAGGACGGCTGAAGCCTTTTCAGACGAAAAAACACAAATTGTTTCAAACACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210671 representing NM_020810
  Red=Cloning site Green=Tags(s)
  
  MVLWILWRPFGFSGRFLKLESHSITESKSLIPVAWTSLTQMLLEAPGIFLLGQRKRFSTMPETETHERET
  ELFSPPSDVRGMTKLDRTAFKKTVNIPVLKVRKEIVSKLMRSLKRAALQRPGIRRVIEDPEDKESRLIML
  DPYKIFTHDSFEKAELSVLEQLNVSPQISKYNLELTYEHFKSEEILRAVLPEGQDVTSGFSRIGHIAHLN
  LRDHQLPFKHLIGQVMIDKNPGITSAVNKINNIDNMYRNFQMEVLSGEQNMMTKVRENNYTYEFDFSKVY
  WNPRLSTEHSRITELLKPGDVLFDVFAGVGPFAIPVAKKNCTVFANDLNPESHKWLLYNCKLNKVDQKVK
  VFNLDGKDFLQGPVKEELMQLLGLSKERKPSVHVVMNLPAKAIEFLSAFKWLLDGQPCSSEFLPIVHCYS
  FSKDANPAEDVRQRAGAVLGISLEACSSVHLVRNVAPNKEMLCITFQIPASVLYKNQTRNPENHEDPPLK
  RQRTAEAFSDEKTQIVSNT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020810
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020810.3, NP_065861.3
• RefSeq Size: 5272 bp
• RefSeq ORF: 1530 bp
• Locus ID: 57570
• UniProt ID: Q32P41
• Cytogenetics: 14q23.1
• MW: 58.1 kDa
• Gene Summary: tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine (Brule et al., 2004 [PubMed 15248782]).[supplied by OMIM, Mar 2008]